Gordonia phage Pipp
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6TG88|A0A5J6TG88_9CAUD Uncharacterized protein OS=Gordonia phage Pipp OX=2599846 GN=76 PE=4 SV=1
MM1 pKa = 7.76 TDD3 pKa = 3.02 NTEE6 pKa = 4.12 PTVPTCSLCRR16 pKa = 11.84 RR17 pKa = 11.84 EE18 pKa = 4.07 RR19 pKa = 11.84 TLADD23 pKa = 3.7 AYY25 pKa = 10.67 DD26 pKa = 4.01 YY27 pKa = 11.75 NPLQVITGQPVGWYY41 pKa = 9.96 SGDD44 pKa = 3.69 DD45 pKa = 3.86 GEE47 pKa = 5.54 VCPQCMANTLNGQVV61 pKa = 3.28
Molecular weight: 6.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.928
IPC2_protein 4.024
IPC_protein 3.859
Toseland 3.668
ProMoST 4.05
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.592
Solomon 3.821
Lehninger 3.783
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.745
EMBOSS 3.834
Sillero 3.986
Patrickios 0.121
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A5J6THW2|A0A5J6THW2_9CAUD Uncharacterized protein OS=Gordonia phage Pipp OX=2599846 GN=8 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 CCAHH6 pKa = 6.75 TGQHH10 pKa = 6.08 LFSFPRR16 pKa = 11.84 RR17 pKa = 11.84 GAAVRR22 pKa = 11.84 APSTPHH28 pKa = 4.56 TRR30 pKa = 11.84 CWAGINRR37 pKa = 11.84 AVTGLPRR44 pKa = 11.84 TGPNTRR50 pKa = 11.84 STRR53 pKa = 11.84 AATGPQPDD61 pKa = 4.52 EE62 pKa = 4.3 NPMARR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 PNPEE73 pKa = 3.24 QRR75 pKa = 11.84 GLGHH79 pKa = 6.69 AHH81 pKa = 6.24 KK82 pKa = 10.55 QQVKK86 pKa = 9.27 HH87 pKa = 5.94 LKK89 pKa = 7.95 TRR91 pKa = 11.84 HH92 pKa = 5.47 VDD94 pKa = 3.6 GTPCWWCGKK103 pKa = 9.42 PMYY106 pKa = 10.3 LDD108 pKa = 3.19 RR109 pKa = 11.84 TRR111 pKa = 11.84 NPDD114 pKa = 3.68 YY115 pKa = 11.24 DD116 pKa = 3.68 PTSPDD121 pKa = 3.22 RR122 pKa = 11.84 ASGSLAGDD130 pKa = 3.23 HH131 pKa = 6.01 TLARR135 pKa = 11.84 THH137 pKa = 6.66 GGTLADD143 pKa = 3.84 RR144 pKa = 11.84 LLHH147 pKa = 5.57 GRR149 pKa = 11.84 CNKK152 pKa = 9.43 EE153 pKa = 3.87 RR154 pKa = 11.84 GDD156 pKa = 3.61 GRR158 pKa = 11.84 HH159 pKa = 6.0 DD160 pKa = 3.33 HH161 pKa = 6.33 TRR163 pKa = 11.84 PAVTGKK169 pKa = 9.2 HH170 pKa = 4.68 QPTPTTHH177 pKa = 7.2 LAINCWPP184 pKa = 3.68
Molecular weight: 20.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.56
IPC_protein 10.657
Toseland 10.891
ProMoST 10.921
Dawson 10.95
Bjellqvist 10.774
Wikipedia 11.242
Rodwell 10.935
Grimsley 10.979
Solomon 11.213
Lehninger 11.155
Nozaki 10.921
DTASelect 10.76
Thurlkill 10.891
EMBOSS 11.33
Sillero 10.906
Patrickios 10.687
IPC_peptide 11.228
IPC2_peptide 10.233
IPC2.peptide.svr19 8.916
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
16686
44
1885
198.6
21.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.65 ± 0.489
0.827 ± 0.135
7.006 ± 0.308
5.502 ± 0.255
2.823 ± 0.134
8.444 ± 0.297
2.307 ± 0.21
4.255 ± 0.154
3.338 ± 0.225
7.695 ± 0.228
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.173
2.895 ± 0.145
5.975 ± 0.265
3.41 ± 0.151
7.545 ± 0.368
5.076 ± 0.206
6.982 ± 0.259
7.707 ± 0.253
1.954 ± 0.134
2.271 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here