Amino acid dipepetide frequency for Gordonia phage Pipp

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.86AlaAla: 17.86 ± 1.093
0.539AlaCys: 0.539 ± 0.193
8.391AlaAsp: 8.391 ± 0.82
5.993AlaGlu: 5.993 ± 0.549
3.896AlaPhe: 3.896 ± 0.631
8.93AlaGly: 8.93 ± 0.819
2.098AlaHis: 2.098 ± 0.406
5.274AlaIle: 5.274 ± 0.5
3.416AlaLys: 3.416 ± 0.711
10.488AlaLeu: 10.488 ± 0.907
2.817AlaMet: 2.817 ± 0.457
3.596AlaAsn: 3.596 ± 0.482
5.154AlaPro: 5.154 ± 0.681
4.195AlaGln: 4.195 ± 0.503
8.031AlaArg: 8.031 ± 0.856
5.514AlaSer: 5.514 ± 0.599
7.552AlaThr: 7.552 ± 0.663
8.391AlaVal: 8.391 ± 0.861
2.098AlaTrp: 2.098 ± 0.356
2.277AlaTyr: 2.277 ± 0.391
0.0AlaXaa: 0.0 ± 0.0
Cys
0.839CysAla: 0.839 ± 0.208
0.24CysCys: 0.24 ± 0.154
0.959CysAsp: 0.959 ± 0.3
0.42CysGlu: 0.42 ± 0.168
0.0CysPhe: 0.0 ± 0.0
0.839CysGly: 0.839 ± 0.202
0.539CysHis: 0.539 ± 0.192
0.06CysIle: 0.06 ± 0.056
0.3CysLys: 0.3 ± 0.128
0.3CysLeu: 0.3 ± 0.134
0.18CysMet: 0.18 ± 0.101
0.42CysAsn: 0.42 ± 0.158
0.899CysPro: 0.899 ± 0.248
0.3CysGln: 0.3 ± 0.132
0.599CysArg: 0.599 ± 0.178
0.24CysSer: 0.24 ± 0.116
0.42CysThr: 0.42 ± 0.174
0.42CysVal: 0.42 ± 0.172
0.3CysTrp: 0.3 ± 0.209
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
7.672AspAla: 7.672 ± 0.674
0.24AspCys: 0.24 ± 0.116
5.993AspAsp: 5.993 ± 0.641
4.975AspGlu: 4.975 ± 0.656
1.918AspPhe: 1.918 ± 0.331
6.353AspGly: 6.353 ± 0.728
2.218AspHis: 2.218 ± 0.344
2.277AspIle: 2.277 ± 0.349
1.858AspLys: 1.858 ± 0.337
6.533AspLeu: 6.533 ± 0.719
1.259AspMet: 1.259 ± 0.267
2.098AspAsn: 2.098 ± 0.376
4.315AspPro: 4.315 ± 0.538
2.697AspGln: 2.697 ± 0.35
4.315AspArg: 4.315 ± 0.761
3.117AspSer: 3.117 ± 0.412
3.177AspThr: 3.177 ± 0.392
6.113AspVal: 6.113 ± 0.626
1.019AspTrp: 1.019 ± 0.213
1.918AspTyr: 1.918 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
4.855GluAla: 4.855 ± 0.667
0.3GluCys: 0.3 ± 0.139
3.117GluAsp: 3.117 ± 0.479
2.218GluGlu: 2.218 ± 0.418
1.978GluPhe: 1.978 ± 0.288
3.956GluGly: 3.956 ± 0.424
1.438GluHis: 1.438 ± 0.268
2.937GluIle: 2.937 ± 0.382
2.337GluLys: 2.337 ± 0.291
5.094GluLeu: 5.094 ± 0.702
1.378GluMet: 1.378 ± 0.255
1.378GluAsn: 1.378 ± 0.252
3.836GluPro: 3.836 ± 0.631
2.637GluGln: 2.637 ± 0.412
4.735GluArg: 4.735 ± 0.489
2.697GluSer: 2.697 ± 0.357
2.997GluThr: 2.997 ± 0.328
4.495GluVal: 4.495 ± 0.444
1.259GluTrp: 1.259 ± 0.298
1.378GluTyr: 1.378 ± 0.28
0.0GluXaa: 0.0 ± 0.0
Phe
2.757PheAla: 2.757 ± 0.359
0.06PheCys: 0.06 ± 0.064
2.098PheAsp: 2.098 ± 0.311
1.558PheGlu: 1.558 ± 0.287
0.839PhePhe: 0.839 ± 0.228
2.457PheGly: 2.457 ± 0.363
0.479PheHis: 0.479 ± 0.215
0.839PheIle: 0.839 ± 0.276
0.959PheLys: 0.959 ± 0.283
2.038PheLeu: 2.038 ± 0.388
0.839PheMet: 0.839 ± 0.189
0.599PheAsn: 0.599 ± 0.201
1.678PhePro: 1.678 ± 0.263
0.659PheGln: 0.659 ± 0.173
1.978PheArg: 1.978 ± 0.302
1.978PheSer: 1.978 ± 0.388
2.397PheThr: 2.397 ± 0.371
2.997PheVal: 2.997 ± 0.4
0.12PheTrp: 0.12 ± 0.08
0.899PheTyr: 0.899 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
8.75GlyAla: 8.75 ± 0.932
0.36GlyCys: 0.36 ± 0.16
5.634GlyAsp: 5.634 ± 0.578
5.094GlyGlu: 5.094 ± 0.673
2.877GlyPhe: 2.877 ± 0.429
7.312GlyGly: 7.312 ± 0.762
1.738GlyHis: 1.738 ± 0.415
3.536GlyIle: 3.536 ± 0.547
3.416GlyLys: 3.416 ± 0.497
7.731GlyLeu: 7.731 ± 1.074
1.858GlyMet: 1.858 ± 0.326
2.637GlyAsn: 2.637 ± 0.323
4.495GlyPro: 4.495 ± 0.496
2.817GlyGln: 2.817 ± 0.39
6.653GlyArg: 6.653 ± 0.561
4.315GlySer: 4.315 ± 0.469
5.094GlyThr: 5.094 ± 0.624
5.574GlyVal: 5.574 ± 0.529
2.158GlyTrp: 2.158 ± 0.327
2.397GlyTyr: 2.397 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
2.697HisAla: 2.697 ± 0.422
0.36HisCys: 0.36 ± 0.152
1.498HisAsp: 1.498 ± 0.301
1.019HisGlu: 1.019 ± 0.226
0.599HisPhe: 0.599 ± 0.196
1.858HisGly: 1.858 ± 0.344
0.479HisHis: 0.479 ± 0.182
1.079HisIle: 1.079 ± 0.299
0.42HisLys: 0.42 ± 0.156
2.218HisLeu: 2.218 ± 0.399
0.36HisMet: 0.36 ± 0.123
0.42HisAsn: 0.42 ± 0.162
1.858HisPro: 1.858 ± 0.376
0.839HisGln: 0.839 ± 0.198
1.858HisArg: 1.858 ± 0.358
0.959HisSer: 0.959 ± 0.22
1.798HisThr: 1.798 ± 0.405
1.798HisVal: 1.798 ± 0.368
0.42HisTrp: 0.42 ± 0.134
0.539HisTyr: 0.539 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
5.694IleAla: 5.694 ± 0.631
0.24IleCys: 0.24 ± 0.127
3.296IleAsp: 3.296 ± 0.521
1.798IleGlu: 1.798 ± 0.293
0.779IlePhe: 0.779 ± 0.221
4.975IleGly: 4.975 ± 0.727
0.779IleHis: 0.779 ± 0.253
1.199IleIle: 1.199 ± 0.276
1.558IleLys: 1.558 ± 0.431
2.577IleLeu: 2.577 ± 0.383
0.24IleMet: 0.24 ± 0.103
1.259IleAsn: 1.259 ± 0.279
2.577IlePro: 2.577 ± 0.373
1.019IleGln: 1.019 ± 0.269
3.536IleArg: 3.536 ± 0.521
2.337IleSer: 2.337 ± 0.409
3.836IleThr: 3.836 ± 0.395
3.836IleVal: 3.836 ± 0.416
0.18IleTrp: 0.18 ± 0.111
0.839IleTyr: 0.839 ± 0.213
0.0IleXaa: 0.0 ± 0.0
Lys
3.956LysAla: 3.956 ± 0.558
0.06LysCys: 0.06 ± 0.052
1.678LysAsp: 1.678 ± 0.323
1.498LysGlu: 1.498 ± 0.326
1.079LysPhe: 1.079 ± 0.271
2.397LysGly: 2.397 ± 0.384
0.599LysHis: 0.599 ± 0.225
1.378LysIle: 1.378 ± 0.35
1.798LysLys: 1.798 ± 0.388
2.877LysLeu: 2.877 ± 0.402
0.779LysMet: 0.779 ± 0.307
1.079LysAsn: 1.079 ± 0.3
2.697LysPro: 2.697 ± 0.401
0.959LysGln: 0.959 ± 0.219
1.918LysArg: 1.918 ± 0.357
2.038LysSer: 2.038 ± 0.325
2.457LysThr: 2.457 ± 0.445
2.517LysVal: 2.517 ± 0.329
0.659LysTrp: 0.659 ± 0.199
0.959LysTyr: 0.959 ± 0.25
0.0LysXaa: 0.0 ± 0.0
Leu
10.429LeuAla: 10.429 ± 0.784
0.599LeuCys: 0.599 ± 0.18
5.694LeuAsp: 5.694 ± 0.705
3.416LeuGlu: 3.416 ± 0.544
2.098LeuPhe: 2.098 ± 0.346
6.773LeuGly: 6.773 ± 0.697
1.259LeuHis: 1.259 ± 0.27
2.997LeuIle: 2.997 ± 0.411
2.038LeuLys: 2.038 ± 0.348
4.555LeuLeu: 4.555 ± 0.596
1.918LeuMet: 1.918 ± 0.385
2.697LeuAsn: 2.697 ± 0.408
4.495LeuPro: 4.495 ± 0.507
1.738LeuGln: 1.738 ± 0.31
5.574LeuArg: 5.574 ± 0.64
4.795LeuSer: 4.795 ± 0.701
5.754LeuThr: 5.754 ± 0.713
6.653LeuVal: 6.653 ± 0.722
1.918LeuTrp: 1.918 ± 0.444
1.558LeuTyr: 1.558 ± 0.275
0.0LeuXaa: 0.0 ± 0.0
Met
3.416MetAla: 3.416 ± 0.53
0.18MetCys: 0.18 ± 0.093
0.839MetAsp: 0.839 ± 0.21
0.899MetGlu: 0.899 ± 0.237
0.779MetPhe: 0.779 ± 0.197
1.798MetGly: 1.798 ± 0.295
0.539MetHis: 0.539 ± 0.166
0.599MetIle: 0.599 ± 0.2
0.539MetLys: 0.539 ± 0.195
1.738MetLeu: 1.738 ± 0.327
0.539MetMet: 0.539 ± 0.182
0.539MetAsn: 0.539 ± 0.16
1.678MetPro: 1.678 ± 0.317
0.719MetGln: 0.719 ± 0.24
2.038MetArg: 2.038 ± 0.443
1.618MetSer: 1.618 ± 0.355
2.997MetThr: 2.997 ± 0.38
0.899MetVal: 0.899 ± 0.245
0.599MetTrp: 0.599 ± 0.197
0.42MetTyr: 0.42 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
2.517AsnAla: 2.517 ± 0.443
0.36AsnCys: 0.36 ± 0.137
2.038AsnAsp: 2.038 ± 0.287
1.139AsnGlu: 1.139 ± 0.267
0.539AsnPhe: 0.539 ± 0.194
3.416AsnGly: 3.416 ± 0.55
0.599AsnHis: 0.599 ± 0.167
0.899AsnIle: 0.899 ± 0.22
0.779AsnLys: 0.779 ± 0.249
2.098AsnLeu: 2.098 ± 0.29
0.659AsnMet: 0.659 ± 0.174
0.659AsnAsn: 0.659 ± 0.182
3.536AsnPro: 3.536 ± 0.463
0.779AsnGln: 0.779 ± 0.183
1.738AsnArg: 1.738 ± 0.421
1.378AsnSer: 1.378 ± 0.279
3.057AsnThr: 3.057 ± 0.485
1.678AsnVal: 1.678 ± 0.345
0.36AsnTrp: 0.36 ± 0.114
0.719AsnTyr: 0.719 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
6.413ProAla: 6.413 ± 0.877
0.899ProCys: 0.899 ± 0.274
5.514ProAsp: 5.514 ± 0.703
4.016ProGlu: 4.016 ± 0.58
1.678ProPhe: 1.678 ± 0.311
4.975ProGly: 4.975 ± 0.599
1.378ProHis: 1.378 ± 0.289
3.057ProIle: 3.057 ± 0.416
2.757ProLys: 2.757 ± 0.346
3.776ProLeu: 3.776 ± 0.473
1.378ProMet: 1.378 ± 0.405
1.678ProAsn: 1.678 ± 0.333
3.296ProPro: 3.296 ± 0.566
2.098ProGln: 2.098 ± 0.359
3.896ProArg: 3.896 ± 0.625
2.997ProSer: 2.997 ± 0.375
4.076ProThr: 4.076 ± 0.503
3.716ProVal: 3.716 ± 0.466
1.079ProTrp: 1.079 ± 0.22
1.079ProTyr: 1.079 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
3.236GlnAla: 3.236 ± 0.565
0.12GlnCys: 0.12 ± 0.087
1.019GlnAsp: 1.019 ± 0.231
1.259GlnGlu: 1.259 ± 0.278
1.319GlnPhe: 1.319 ± 0.313
2.158GlnGly: 2.158 ± 0.421
1.259GlnHis: 1.259 ± 0.309
1.798GlnIle: 1.798 ± 0.295
0.959GlnLys: 0.959 ± 0.17
3.236GlnLeu: 3.236 ± 0.409
1.079GlnMet: 1.079 ± 0.307
0.779GlnAsn: 0.779 ± 0.215
2.817GlnPro: 2.817 ± 0.439
1.918GlnGln: 1.918 ± 0.368
2.877GlnArg: 2.877 ± 0.388
1.738GlnSer: 1.738 ± 0.344
1.918GlnThr: 1.918 ± 0.328
2.757GlnVal: 2.757 ± 0.431
0.779GlnTrp: 0.779 ± 0.254
1.079GlnTyr: 1.079 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
8.271ArgAla: 8.271 ± 0.74
1.079ArgCys: 1.079 ± 0.306
5.814ArgAsp: 5.814 ± 0.675
4.495ArgGlu: 4.495 ± 0.548
1.738ArgPhe: 1.738 ± 0.29
6.473ArgGly: 6.473 ± 0.62
2.577ArgHis: 2.577 ± 0.438
3.476ArgIle: 3.476 ± 0.434
2.158ArgLys: 2.158 ± 0.293
5.214ArgLeu: 5.214 ± 0.478
2.457ArgMet: 2.457 ± 0.351
2.158ArgAsn: 2.158 ± 0.343
3.476ArgPro: 3.476 ± 0.422
2.877ArgGln: 2.877 ± 0.466
6.713ArgArg: 6.713 ± 0.827
3.656ArgSer: 3.656 ± 0.401
4.435ArgThr: 4.435 ± 0.5
5.034ArgVal: 5.034 ± 0.552
1.798ArgTrp: 1.798 ± 0.336
1.558ArgTyr: 1.558 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
5.874SerAla: 5.874 ± 0.745
0.3SerCys: 0.3 ± 0.172
3.117SerAsp: 3.117 ± 0.509
3.177SerGlu: 3.177 ± 0.358
1.378SerPhe: 1.378 ± 0.32
5.694SerGly: 5.694 ± 0.699
0.779SerHis: 0.779 ± 0.204
2.577SerIle: 2.577 ± 0.394
1.678SerLys: 1.678 ± 0.349
3.177SerLeu: 3.177 ± 0.433
1.259SerMet: 1.259 ± 0.233
1.558SerAsn: 1.558 ± 0.299
2.697SerPro: 2.697 ± 0.401
1.678SerGln: 1.678 ± 0.416
3.356SerArg: 3.356 ± 0.493
2.337SerSer: 2.337 ± 0.457
4.315SerThr: 4.315 ± 0.507
3.836SerVal: 3.836 ± 0.436
1.199SerTrp: 1.199 ± 0.245
0.779SerTyr: 0.779 ± 0.224
0.0SerXaa: 0.0 ± 0.0
Thr
8.69ThrAla: 8.69 ± 0.848
0.539ThrCys: 0.539 ± 0.187
4.855ThrAsp: 4.855 ± 0.622
4.435ThrGlu: 4.435 ± 0.553
2.397ThrPhe: 2.397 ± 0.491
5.874ThrGly: 5.874 ± 0.616
1.438ThrHis: 1.438 ± 0.352
3.416ThrIle: 3.416 ± 0.421
2.577ThrLys: 2.577 ± 0.447
5.214ThrLeu: 5.214 ± 0.666
1.139ThrMet: 1.139 ± 0.239
1.918ThrAsn: 1.918 ± 0.356
3.896ThrPro: 3.896 ± 0.598
2.038ThrGln: 2.038 ± 0.308
5.394ThrArg: 5.394 ± 0.57
2.997ThrSer: 2.997 ± 0.42
5.094ThrThr: 5.094 ± 0.606
5.094ThrVal: 5.094 ± 0.515
1.199ThrTrp: 1.199 ± 0.258
1.618ThrTyr: 1.618 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
8.451ValAla: 8.451 ± 0.708
1.139ValCys: 1.139 ± 0.307
6.233ValAsp: 6.233 ± 0.822
5.094ValGlu: 5.094 ± 0.665
1.378ValPhe: 1.378 ± 0.284
5.454ValGly: 5.454 ± 0.623
1.438ValHis: 1.438 ± 0.383
3.836ValIle: 3.836 ± 0.448
2.577ValLys: 2.577 ± 0.309
4.315ValLeu: 4.315 ± 0.383
2.218ValMet: 2.218 ± 0.358
1.798ValAsn: 1.798 ± 0.339
3.716ValPro: 3.716 ± 0.417
2.697ValGln: 2.697 ± 0.391
6.113ValArg: 6.113 ± 0.6
3.536ValSer: 3.536 ± 0.545
5.874ValThr: 5.874 ± 0.703
7.851ValVal: 7.851 ± 0.639
1.558ValTrp: 1.558 ± 0.32
1.798ValTyr: 1.798 ± 0.313
0.0ValXaa: 0.0 ± 0.0
Trp
1.618TrpAla: 1.618 ± 0.323
0.42TrpCys: 0.42 ± 0.163
0.959TrpAsp: 0.959 ± 0.283
0.779TrpGlu: 0.779 ± 0.233
0.479TrpPhe: 0.479 ± 0.208
1.019TrpGly: 1.019 ± 0.291
0.659TrpHis: 0.659 ± 0.189
0.539TrpIle: 0.539 ± 0.158
0.599TrpLys: 0.599 ± 0.19
2.277TrpLeu: 2.277 ± 0.42
0.599TrpMet: 0.599 ± 0.198
0.839TrpAsn: 0.839 ± 0.367
1.319TrpPro: 1.319 ± 0.248
0.779TrpGln: 0.779 ± 0.195
1.738TrpArg: 1.738 ± 0.268
1.319TrpSer: 1.319 ± 0.311
1.139TrpThr: 1.139 ± 0.242
1.558TrpVal: 1.558 ± 0.296
0.479TrpTrp: 0.479 ± 0.15
0.36TrpTyr: 0.36 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.517TyrAla: 2.517 ± 0.39
0.24TyrCys: 0.24 ± 0.116
1.319TyrAsp: 1.319 ± 0.228
1.738TyrGlu: 1.738 ± 0.32
0.479TyrPhe: 0.479 ± 0.187
1.738TyrGly: 1.738 ± 0.287
0.779TyrHis: 0.779 ± 0.23
0.779TyrIle: 0.779 ± 0.227
0.659TyrLys: 0.659 ± 0.193
1.498TyrLeu: 1.498 ± 0.264
0.36TyrMet: 0.36 ± 0.151
0.839TyrAsn: 0.839 ± 0.215
1.319TyrPro: 1.319 ± 0.261
0.659TyrGln: 0.659 ± 0.207
2.397TyrArg: 2.397 ± 0.391
1.199TyrSer: 1.199 ± 0.3
1.438TyrThr: 1.438 ± 0.241
1.858TyrVal: 1.858 ± 0.324
0.36TyrTrp: 0.36 ± 0.15
0.539TyrTyr: 0.539 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (16686 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski