Ectothiorhodospira magna

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Ectothiorhodospira

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9ANG4|A0A1H9ANG4_9GAMM DUF5610 domain-containing protein OS=Ectothiorhodospira magna OX=867345 GN=SAMN05421693_1062 PE=4 SV=1
MM1 pKa = 7.56HH2 pKa = 6.9STFKK6 pKa = 10.73KK7 pKa = 10.34AAITSAVTAALLAGGLVTSTASAEE31 pKa = 3.63ISANIGLTSNYY42 pKa = 9.49LFRR45 pKa = 11.84GVSEE49 pKa = 4.3SDD51 pKa = 3.12DD52 pKa = 3.35RR53 pKa = 11.84AAIQGGIDD61 pKa = 3.66FEE63 pKa = 4.98HH64 pKa = 7.01EE65 pKa = 3.89GGFYY69 pKa = 10.58LGTWTSSLDD78 pKa = 3.35GSADD82 pKa = 3.47RR83 pKa = 11.84GGYY86 pKa = 9.38EE87 pKa = 3.46VDD89 pKa = 4.26LYY91 pKa = 11.29FGVAGEE97 pKa = 4.31MGGSGVGFDD106 pKa = 3.57VGYY109 pKa = 10.27IYY111 pKa = 10.58YY112 pKa = 9.49AYY114 pKa = 9.5PGAEE118 pKa = 4.2SADD121 pKa = 3.33DD122 pKa = 4.01ANFGEE127 pKa = 5.38LYY129 pKa = 10.61GALDD133 pKa = 3.51FMGLYY138 pKa = 11.06GMINYY143 pKa = 6.98VTNADD148 pKa = 4.0DD149 pKa = 4.61SDD151 pKa = 4.71LEE153 pKa = 4.14SSLAYY158 pKa = 10.05EE159 pKa = 4.1IGYY162 pKa = 10.04SFGATPDD169 pKa = 3.2IALGATIGHH178 pKa = 5.95VNWDD182 pKa = 3.2HH183 pKa = 7.47RR184 pKa = 11.84DD185 pKa = 3.29WEE187 pKa = 4.79DD188 pKa = 3.52YY189 pKa = 9.56TWWSLYY195 pKa = 8.04LTKK198 pKa = 10.0ATAMGDD204 pKa = 3.22FTFAYY209 pKa = 7.04TQNDD213 pKa = 3.53TDD215 pKa = 4.15EE216 pKa = 6.5DD217 pKa = 4.4DD218 pKa = 4.62DD219 pKa = 4.15PRR221 pKa = 11.84FNVSFVMSFF230 pKa = 2.88

Molecular weight: 24.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9BUR9|A0A1H9BUR9_9GAMM Putative transcriptional regulator OS=Ectothiorhodospira magna OX=867345 GN=SAMN05421693_1115 PE=4 SV=1
MM1 pKa = 7.1SRR3 pKa = 11.84SVRR6 pKa = 11.84IRR8 pKa = 11.84RR9 pKa = 11.84LSEE12 pKa = 3.55MMRR15 pKa = 11.84TRR17 pKa = 11.84LPIFSLRR24 pKa = 11.84IHH26 pKa = 6.41SKK28 pKa = 9.99ILSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLNPSRR74 pKa = 11.84HH75 pKa = 4.86LHH77 pKa = 6.18LSHH80 pKa = 6.91LHH82 pKa = 6.26LSHH85 pKa = 6.91LHH87 pKa = 6.11LSHH90 pKa = 6.94LHH92 pKa = 5.98FSHH95 pKa = 7.75PLRR98 pKa = 11.84SRR100 pKa = 11.84LQMSSLIQQQVRR112 pKa = 11.84RR113 pKa = 11.84WLVGTRR119 pKa = 11.84RR120 pKa = 11.84PLPTTPLL127 pKa = 3.45

Molecular weight: 14.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2463

0

2463

827883

25

2129

336.1

37.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.459 ± 0.055

0.969 ± 0.016

5.865 ± 0.035

6.008 ± 0.046

3.385 ± 0.032

8.057 ± 0.042

2.703 ± 0.026

4.984 ± 0.036

2.705 ± 0.042

11.321 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.022

2.598 ± 0.03

5.239 ± 0.035

4.333 ± 0.036

7.764 ± 0.052

4.861 ± 0.03

5.211 ± 0.033

7.316 ± 0.042

1.317 ± 0.021

2.274 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski