Microbacterium phage Camille

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UIL1|A0A2U8UIL1_9CAUD Uncharacterized protein OS=Microbacterium phage Camille OX=2182351 GN=49 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.5GEE4 pKa = 4.11PTMSYY9 pKa = 7.22TTHH12 pKa = 6.52KK13 pKa = 9.53QRR15 pKa = 11.84KK16 pKa = 6.89IGKK19 pKa = 8.08MVLTTLVIFALVVIAIMVAGCSTPAAPSADD49 pKa = 3.4PKK51 pKa = 10.81ASQAAAPAPVEE62 pKa = 4.13EE63 pKa = 4.56TEE65 pKa = 4.1EE66 pKa = 4.52PEE68 pKa = 4.36VVVSGNYY75 pKa = 9.99SFGDD79 pKa = 3.39VVTFEE84 pKa = 5.6DD85 pKa = 4.07NLSLSVSAPAPYY97 pKa = 9.61TPSEE101 pKa = 4.14YY102 pKa = 10.51AAGVVEE108 pKa = 4.52GQQVLLFEE116 pKa = 4.93FVLTNNTGEE125 pKa = 4.32NFDD128 pKa = 3.84PTLVYY133 pKa = 9.68ATASSGGVEE142 pKa = 4.11AGSVFDD148 pKa = 3.85TGEE151 pKa = 4.47GIDD154 pKa = 4.42FNPTTAILPGQTVKK168 pKa = 10.03WKK170 pKa = 9.96QAWSVADD177 pKa = 3.73PAAITMEE184 pKa = 3.66ISVGFEE190 pKa = 3.5YY191 pKa = 10.96DD192 pKa = 5.04DD193 pKa = 5.36IIFTNIQQ200 pKa = 2.88

Molecular weight: 21.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W0Y5|A0A4P8W0Y5_9CAUD Uncharacterized protein OS=Microbacterium phage Camille OX=2182351 GN=81 PE=4 SV=1
MM1 pKa = 7.39SHH3 pKa = 6.9RR4 pKa = 11.84KK5 pKa = 9.48YY6 pKa = 9.59KK7 pKa = 9.24TNLMVARR14 pKa = 11.84YY15 pKa = 9.09GFGIGMLMICLAIVMSKK32 pKa = 10.67GG33 pKa = 3.15

Molecular weight: 3.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

16798

33

1402

207.4

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.001 ± 0.604

0.464 ± 0.092

6.263 ± 0.342

7.191 ± 0.371

3.703 ± 0.201

7.912 ± 0.488

1.709 ± 0.211

5.917 ± 0.253

6.09 ± 0.385

8.031 ± 0.384

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.302

4.173 ± 0.157

4.167 ± 0.276

3.435 ± 0.165

5.411 ± 0.362

5.745 ± 0.241

6.209 ± 0.223

6.632 ± 0.243

1.536 ± 0.131

3.614 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski