Amino acid dipepetide frequency for Microbacterium phage Camille

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.871AlaAla: 8.871 ± 1.423
0.179AlaCys: 0.179 ± 0.099
5.477AlaAsp: 5.477 ± 0.684
5.953AlaGlu: 5.953 ± 0.518
3.036AlaPhe: 3.036 ± 0.387
6.906AlaGly: 6.906 ± 1.19
1.488AlaHis: 1.488 ± 0.322
6.608AlaIle: 6.608 ± 0.705
5.12AlaLys: 5.12 ± 0.724
7.382AlaLeu: 7.382 ± 1.135
3.513AlaMet: 3.513 ± 0.907
3.334AlaAsn: 3.334 ± 0.48
2.977AlaPro: 2.977 ± 0.597
3.334AlaGln: 3.334 ± 0.51
6.43AlaArg: 6.43 ± 0.78
4.822AlaSer: 4.822 ± 0.684
4.882AlaThr: 4.882 ± 0.469
5.656AlaVal: 5.656 ± 0.767
1.31AlaTrp: 1.31 ± 0.246
2.739AlaTyr: 2.739 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
0.298CysAla: 0.298 ± 0.153
0.119CysCys: 0.119 ± 0.077
0.536CysAsp: 0.536 ± 0.203
0.298CysGlu: 0.298 ± 0.118
0.119CysPhe: 0.119 ± 0.077
0.179CysGly: 0.179 ± 0.105
0.06CysHis: 0.06 ± 0.067
0.417CysIle: 0.417 ± 0.162
0.119CysLys: 0.119 ± 0.092
0.595CysLeu: 0.595 ± 0.198
0.119CysMet: 0.119 ± 0.082
0.179CysAsn: 0.179 ± 0.101
0.238CysPro: 0.238 ± 0.132
0.357CysGln: 0.357 ± 0.153
0.238CysArg: 0.238 ± 0.104
0.357CysSer: 0.357 ± 0.152
0.06CysThr: 0.06 ± 0.062
0.119CysVal: 0.119 ± 0.078
0.119CysTrp: 0.119 ± 0.073
0.119CysTyr: 0.119 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
6.132AspAla: 6.132 ± 0.729
0.238AspCys: 0.238 ± 0.114
4.167AspAsp: 4.167 ± 0.682
6.073AspGlu: 6.073 ± 0.534
2.441AspPhe: 2.441 ± 0.369
5.715AspGly: 5.715 ± 0.72
0.953AspHis: 0.953 ± 0.218
4.108AspIle: 4.108 ± 0.621
2.739AspLys: 2.739 ± 0.408
4.822AspLeu: 4.822 ± 0.605
1.786AspMet: 1.786 ± 0.327
2.679AspAsn: 2.679 ± 0.386
3.453AspPro: 3.453 ± 0.584
2.203AspGln: 2.203 ± 0.437
2.798AspArg: 2.798 ± 0.376
2.262AspSer: 2.262 ± 0.348
3.393AspThr: 3.393 ± 0.394
3.751AspVal: 3.751 ± 0.446
0.893AspTrp: 0.893 ± 0.215
2.024AspTyr: 2.024 ± 0.38
0.0AspXaa: 0.0 ± 0.0
Glu
6.846GluAla: 6.846 ± 0.759
0.238GluCys: 0.238 ± 0.116
3.691GluAsp: 3.691 ± 0.511
6.132GluGlu: 6.132 ± 0.742
2.322GluPhe: 2.322 ± 0.389
4.406GluGly: 4.406 ± 0.552
0.714GluHis: 0.714 ± 0.24
4.286GluIle: 4.286 ± 0.454
4.525GluLys: 4.525 ± 0.665
6.668GluLeu: 6.668 ± 0.603
2.5GluMet: 2.5 ± 0.358
3.632GluAsn: 3.632 ± 0.488
2.62GluPro: 2.62 ± 0.457
2.679GluGln: 2.679 ± 0.42
3.513GluArg: 3.513 ± 0.446
3.036GluSer: 3.036 ± 0.522
4.763GluThr: 4.763 ± 0.575
5.239GluVal: 5.239 ± 0.585
1.667GluTrp: 1.667 ± 0.337
2.441GluTyr: 2.441 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.56PheAla: 2.56 ± 0.533
0.298PheCys: 0.298 ± 0.127
2.977PheAsp: 2.977 ± 0.298
3.215PheGlu: 3.215 ± 0.481
1.607PhePhe: 1.607 ± 0.304
4.346PheGly: 4.346 ± 0.606
0.536PheHis: 0.536 ± 0.236
1.607PheIle: 1.607 ± 0.376
2.084PheLys: 2.084 ± 0.405
2.381PheLeu: 2.381 ± 0.342
0.595PheMet: 0.595 ± 0.22
1.965PheAsn: 1.965 ± 0.387
1.072PhePro: 1.072 ± 0.233
1.012PheGln: 1.012 ± 0.299
2.203PheArg: 2.203 ± 0.423
1.905PheSer: 1.905 ± 0.391
1.786PheThr: 1.786 ± 0.305
2.62PheVal: 2.62 ± 0.474
0.357PheTrp: 0.357 ± 0.131
1.905PheTyr: 1.905 ± 0.373
0.0PheXaa: 0.0 ± 0.0
Gly
7.263GlyAla: 7.263 ± 1.185
0.595GlyCys: 0.595 ± 0.196
5.299GlyAsp: 5.299 ± 0.653
5.358GlyGlu: 5.358 ± 0.562
3.334GlyPhe: 3.334 ± 0.433
6.906GlyGly: 6.906 ± 1.842
1.25GlyHis: 1.25 ± 0.318
5.179GlyIle: 5.179 ± 0.728
3.751GlyLys: 3.751 ± 0.613
6.192GlyLeu: 6.192 ± 0.885
1.726GlyMet: 1.726 ± 0.43
2.084GlyAsn: 2.084 ± 0.345
2.203GlyPro: 2.203 ± 0.422
2.322GlyGln: 2.322 ± 0.529
3.274GlyArg: 3.274 ± 0.505
5.477GlySer: 5.477 ± 0.678
5.953GlyThr: 5.953 ± 0.771
5.477GlyVal: 5.477 ± 0.605
1.726GlyTrp: 1.726 ± 0.311
3.691GlyTyr: 3.691 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
0.714HisAla: 0.714 ± 0.215
0.06HisCys: 0.06 ± 0.053
1.131HisAsp: 1.131 ± 0.307
1.131HisGlu: 1.131 ± 0.242
0.655HisPhe: 0.655 ± 0.196
1.488HisGly: 1.488 ± 0.369
0.357HisHis: 0.357 ± 0.155
1.191HisIle: 1.191 ± 0.239
1.191HisLys: 1.191 ± 0.245
1.905HisLeu: 1.905 ± 0.376
0.476HisMet: 0.476 ± 0.209
0.536HisAsn: 0.536 ± 0.189
1.072HisPro: 1.072 ± 0.301
0.833HisGln: 0.833 ± 0.202
0.595HisArg: 0.595 ± 0.191
0.595HisSer: 0.595 ± 0.237
0.714HisThr: 0.714 ± 0.189
0.953HisVal: 0.953 ± 0.25
0.417HisTrp: 0.417 ± 0.186
1.072HisTyr: 1.072 ± 0.301
0.0HisXaa: 0.0 ± 0.0
Ile
5.358IleAla: 5.358 ± 0.623
0.179IleCys: 0.179 ± 0.12
4.584IleAsp: 4.584 ± 0.559
4.167IleGlu: 4.167 ± 0.505
2.381IlePhe: 2.381 ± 0.34
4.525IleGly: 4.525 ± 0.952
1.012IleHis: 1.012 ± 0.323
3.274IleIle: 3.274 ± 0.63
4.703IleLys: 4.703 ± 0.63
5.239IleLeu: 5.239 ± 0.592
1.31IleMet: 1.31 ± 0.319
1.905IleAsn: 1.905 ± 0.357
2.62IlePro: 2.62 ± 0.313
1.726IleGln: 1.726 ± 0.301
3.036IleArg: 3.036 ± 0.467
3.393IleSer: 3.393 ± 0.407
3.274IleThr: 3.274 ± 0.531
4.763IleVal: 4.763 ± 0.566
0.595IleTrp: 0.595 ± 0.19
1.131IleTyr: 1.131 ± 0.304
0.0IleXaa: 0.0 ± 0.0
Lys
5.358LysAla: 5.358 ± 0.761
0.179LysCys: 0.179 ± 0.135
2.917LysAsp: 2.917 ± 0.519
4.525LysGlu: 4.525 ± 0.541
2.203LysPhe: 2.203 ± 0.534
3.751LysGly: 3.751 ± 0.5
1.726LysHis: 1.726 ± 0.344
3.393LysIle: 3.393 ± 0.431
4.703LysLys: 4.703 ± 0.763
5.12LysLeu: 5.12 ± 0.683
2.143LysMet: 2.143 ± 0.349
3.334LysAsn: 3.334 ± 0.594
2.62LysPro: 2.62 ± 0.409
2.62LysGln: 2.62 ± 0.538
2.5LysArg: 2.5 ± 0.508
3.453LysSer: 3.453 ± 0.554
3.632LysThr: 3.632 ± 0.508
3.87LysVal: 3.87 ± 0.525
0.893LysTrp: 0.893 ± 0.224
1.965LysTyr: 1.965 ± 0.388
0.0LysXaa: 0.0 ± 0.0
Leu
7.085LeuAla: 7.085 ± 1.198
0.417LeuCys: 0.417 ± 0.147
5.239LeuAsp: 5.239 ± 0.533
5.834LeuGlu: 5.834 ± 0.59
2.739LeuPhe: 2.739 ± 0.373
5.715LeuGly: 5.715 ± 0.678
1.25LeuHis: 1.25 ± 0.259
3.989LeuIle: 3.989 ± 0.436
5.06LeuLys: 5.06 ± 0.677
5.001LeuLeu: 5.001 ± 0.771
2.858LeuMet: 2.858 ± 0.455
3.572LeuAsn: 3.572 ± 0.397
3.453LeuPro: 3.453 ± 0.519
2.798LeuGln: 2.798 ± 0.346
4.644LeuArg: 4.644 ± 0.594
4.822LeuSer: 4.822 ± 0.549
5.656LeuThr: 5.656 ± 0.675
5.834LeuVal: 5.834 ± 0.687
1.131LeuTrp: 1.131 ± 0.296
3.215LeuTyr: 3.215 ± 0.499
0.0LeuXaa: 0.0 ± 0.0
Met
2.917MetAla: 2.917 ± 0.625
0.179MetCys: 0.179 ± 0.124
1.429MetAsp: 1.429 ± 0.342
1.905MetGlu: 1.905 ± 0.272
0.714MetPhe: 0.714 ± 0.272
2.203MetGly: 2.203 ± 0.472
0.357MetHis: 0.357 ± 0.147
1.429MetIle: 1.429 ± 0.275
1.786MetLys: 1.786 ± 0.319
1.846MetLeu: 1.846 ± 0.333
0.893MetMet: 0.893 ± 0.24
1.072MetAsn: 1.072 ± 0.215
1.369MetPro: 1.369 ± 0.286
0.714MetGln: 0.714 ± 0.273
1.846MetArg: 1.846 ± 0.312
2.262MetSer: 2.262 ± 0.406
2.322MetThr: 2.322 ± 0.388
1.846MetVal: 1.846 ± 0.305
0.238MetTrp: 0.238 ± 0.112
0.655MetTyr: 0.655 ± 0.191
0.0MetXaa: 0.0 ± 0.0
Asn
3.453AsnAla: 3.453 ± 0.418
0.357AsnCys: 0.357 ± 0.169
2.62AsnAsp: 2.62 ± 0.468
3.453AsnGlu: 3.453 ± 0.623
1.667AsnPhe: 1.667 ± 0.325
3.81AsnGly: 3.81 ± 0.456
0.655AsnHis: 0.655 ± 0.169
1.905AsnIle: 1.905 ± 0.278
2.441AsnLys: 2.441 ± 0.381
3.215AsnLeu: 3.215 ± 0.455
0.893AsnMet: 0.893 ± 0.332
1.548AsnAsn: 1.548 ± 0.279
2.262AsnPro: 2.262 ± 0.419
1.31AsnGln: 1.31 ± 0.256
2.441AsnArg: 2.441 ± 0.476
2.739AsnSer: 2.739 ± 0.354
2.56AsnThr: 2.56 ± 0.33
2.739AsnVal: 2.739 ± 0.385
0.417AsnTrp: 0.417 ± 0.172
1.25AsnTyr: 1.25 ± 0.315
0.0AsnXaa: 0.0 ± 0.0
Pro
3.572ProAla: 3.572 ± 0.508
0.119ProCys: 0.119 ± 0.086
2.858ProAsp: 2.858 ± 0.386
2.798ProGlu: 2.798 ± 0.423
1.667ProPhe: 1.667 ± 0.334
3.155ProGly: 3.155 ± 0.536
0.833ProHis: 0.833 ± 0.261
1.905ProIle: 1.905 ± 0.357
3.215ProLys: 3.215 ± 0.412
2.5ProLeu: 2.5 ± 0.42
1.31ProMet: 1.31 ± 0.256
1.667ProAsn: 1.667 ± 0.344
1.667ProPro: 1.667 ± 0.266
1.31ProGln: 1.31 ± 0.304
2.739ProArg: 2.739 ± 0.423
3.215ProSer: 3.215 ± 0.541
2.381ProThr: 2.381 ± 0.378
2.917ProVal: 2.917 ± 0.37
0.476ProTrp: 0.476 ± 0.165
1.369ProTyr: 1.369 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
3.453GlnAla: 3.453 ± 0.495
0.179GlnCys: 0.179 ± 0.092
1.786GlnAsp: 1.786 ± 0.269
1.846GlnGlu: 1.846 ± 0.267
1.191GlnPhe: 1.191 ± 0.268
2.262GlnGly: 2.262 ± 0.374
0.893GlnHis: 0.893 ± 0.3
2.084GlnIle: 2.084 ± 0.356
2.62GlnLys: 2.62 ± 0.452
3.036GlnLeu: 3.036 ± 0.731
0.774GlnMet: 0.774 ± 0.189
1.667GlnAsn: 1.667 ± 0.336
1.31GlnPro: 1.31 ± 0.354
1.667GlnGln: 1.667 ± 0.39
2.203GlnArg: 2.203 ± 0.418
1.488GlnSer: 1.488 ± 0.334
1.667GlnThr: 1.667 ± 0.333
2.381GlnVal: 2.381 ± 0.339
0.536GlnTrp: 0.536 ± 0.182
1.31GlnTyr: 1.31 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
4.406ArgAla: 4.406 ± 0.518
0.06ArgCys: 0.06 ± 0.053
3.155ArgAsp: 3.155 ± 0.509
3.989ArgGlu: 3.989 ± 0.53
2.084ArgPhe: 2.084 ± 0.411
3.87ArgGly: 3.87 ± 0.543
1.072ArgHis: 1.072 ± 0.295
2.679ArgIle: 2.679 ± 0.356
3.691ArgLys: 3.691 ± 0.456
4.465ArgLeu: 4.465 ± 0.502
1.25ArgMet: 1.25 ± 0.311
2.739ArgAsn: 2.739 ± 0.452
1.905ArgPro: 1.905 ± 0.366
1.548ArgGln: 1.548 ± 0.363
3.393ArgArg: 3.393 ± 0.535
3.334ArgSer: 3.334 ± 0.46
4.048ArgThr: 4.048 ± 0.584
3.393ArgVal: 3.393 ± 0.466
0.714ArgTrp: 0.714 ± 0.228
2.322ArgTyr: 2.322 ± 0.326
0.0ArgXaa: 0.0 ± 0.0
Ser
5.001SerAla: 5.001 ± 0.675
0.298SerCys: 0.298 ± 0.126
2.739SerAsp: 2.739 ± 0.397
2.917SerGlu: 2.917 ± 0.338
2.203SerPhe: 2.203 ± 0.477
5.418SerGly: 5.418 ± 0.736
0.714SerHis: 0.714 ± 0.218
3.215SerIle: 3.215 ± 0.472
3.572SerLys: 3.572 ± 0.507
6.013SerLeu: 6.013 ± 0.672
1.846SerMet: 1.846 ± 0.315
2.262SerAsn: 2.262 ± 0.362
2.62SerPro: 2.62 ± 0.466
1.965SerGln: 1.965 ± 0.354
2.739SerArg: 2.739 ± 0.376
3.87SerSer: 3.87 ± 0.562
3.334SerThr: 3.334 ± 0.506
3.393SerVal: 3.393 ± 0.499
1.25SerTrp: 1.25 ± 0.309
2.084SerTyr: 2.084 ± 0.372
0.0SerXaa: 0.0 ± 0.0
Thr
6.073ThrAla: 6.073 ± 0.69
0.357ThrCys: 0.357 ± 0.154
4.286ThrAsp: 4.286 ± 0.499
3.453ThrGlu: 3.453 ± 0.418
2.5ThrPhe: 2.5 ± 0.464
5.358ThrGly: 5.358 ± 0.641
1.072ThrHis: 1.072 ± 0.31
4.286ThrIle: 4.286 ± 0.503
3.215ThrLys: 3.215 ± 0.339
5.418ThrLeu: 5.418 ± 0.668
1.369ThrMet: 1.369 ± 0.354
2.441ThrAsn: 2.441 ± 0.35
3.393ThrPro: 3.393 ± 0.417
1.726ThrGln: 1.726 ± 0.315
3.036ThrArg: 3.036 ± 0.492
3.036ThrSer: 3.036 ± 0.422
3.87ThrThr: 3.87 ± 0.666
3.929ThrVal: 3.929 ± 0.528
1.191ThrTrp: 1.191 ± 0.301
2.084ThrTyr: 2.084 ± 0.365
0.0ThrXaa: 0.0 ± 0.0
Val
6.192ValAla: 6.192 ± 0.519
0.238ValCys: 0.238 ± 0.12
4.227ValAsp: 4.227 ± 0.414
4.941ValGlu: 4.941 ± 0.609
2.084ValPhe: 2.084 ± 0.379
4.941ValGly: 4.941 ± 0.607
0.833ValHis: 0.833 ± 0.218
4.465ValIle: 4.465 ± 0.45
3.632ValLys: 3.632 ± 0.501
4.703ValLeu: 4.703 ± 0.576
1.429ValMet: 1.429 ± 0.304
2.679ValAsn: 2.679 ± 0.35
3.334ValPro: 3.334 ± 0.421
2.381ValGln: 2.381 ± 0.327
3.513ValArg: 3.513 ± 0.459
4.048ValSer: 4.048 ± 0.488
4.286ValThr: 4.286 ± 0.632
4.703ValVal: 4.703 ± 0.711
0.774ValTrp: 0.774 ± 0.218
2.917ValTyr: 2.917 ± 0.451
0.0ValXaa: 0.0 ± 0.0
Trp
1.25TrpAla: 1.25 ± 0.257
0.06TrpCys: 0.06 ± 0.068
1.25TrpAsp: 1.25 ± 0.369
1.072TrpGlu: 1.072 ± 0.223
0.655TrpPhe: 0.655 ± 0.19
1.191TrpGly: 1.191 ± 0.295
0.417TrpHis: 0.417 ± 0.146
0.774TrpIle: 0.774 ± 0.24
0.833TrpLys: 0.833 ± 0.293
0.953TrpLeu: 0.953 ± 0.228
0.595TrpMet: 0.595 ± 0.207
0.774TrpAsn: 0.774 ± 0.24
0.536TrpPro: 0.536 ± 0.16
0.476TrpGln: 0.476 ± 0.18
0.595TrpArg: 0.595 ± 0.18
0.953TrpSer: 0.953 ± 0.27
1.31TrpThr: 1.31 ± 0.234
0.893TrpVal: 0.893 ± 0.199
0.179TrpTrp: 0.179 ± 0.095
0.595TrpTyr: 0.595 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.215TyrAla: 3.215 ± 0.516
0.298TyrCys: 0.298 ± 0.111
2.262TyrAsp: 2.262 ± 0.455
2.858TyrGlu: 2.858 ± 0.565
1.429TyrPhe: 1.429 ± 0.316
2.977TyrGly: 2.977 ± 0.388
0.893TyrHis: 0.893 ± 0.213
2.381TyrIle: 2.381 ± 0.457
1.905TyrLys: 1.905 ± 0.476
2.858TyrLeu: 2.858 ± 0.4
0.536TyrMet: 0.536 ± 0.162
1.667TyrAsn: 1.667 ± 0.361
0.953TyrPro: 0.953 ± 0.267
1.369TyrGln: 1.369 ± 0.287
2.381TyrArg: 2.381 ± 0.409
2.381TyrSer: 2.381 ± 0.494
2.203TyrThr: 2.203 ± 0.353
1.846TyrVal: 1.846 ± 0.285
0.476TyrTrp: 0.476 ± 0.199
1.25TyrTyr: 1.25 ± 0.304
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (16798 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski