Maioricimonas rarisocia
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5804 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A517Z9A8|A0A517Z9A8_9PLAN L-rhamnonate dehydratase OS=Maioricimonas rarisocia OX=2528026 GN=rhmD_1 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 GDD4 pKa = 3.95 CGKK7 pKa = 10.32 AWSHH11 pKa = 6.19 IGAMVLCASLSAGCGGGNDD30 pKa = 4.14 GPEE33 pKa = 4.65 LIDD36 pKa = 3.49 VYY38 pKa = 10.85 GTVTMDD44 pKa = 3.54 GQPLEE49 pKa = 4.39 GATVQFAPEE58 pKa = 4.1 GGAGEE63 pKa = 4.3 GRR65 pKa = 11.84 RR66 pKa = 11.84 LVAGRR71 pKa = 11.84 TDD73 pKa = 3.08 SSGEE77 pKa = 3.72 YY78 pKa = 9.87 SLQYY82 pKa = 10.62 SPSRR86 pKa = 11.84 DD87 pKa = 3.06 GAAPGSYY94 pKa = 8.93 RR95 pKa = 11.84 VSITTYY101 pKa = 10.52 RR102 pKa = 11.84 EE103 pKa = 3.79 MEE105 pKa = 3.94 VDD107 pKa = 3.74 EE108 pKa = 5.43 EE109 pKa = 4.75 GMDD112 pKa = 3.71 VPGSPEE118 pKa = 4.02 TVPDD122 pKa = 3.79 VYY124 pKa = 11.43 NVNTTLAADD133 pKa = 3.83 VSSDD137 pKa = 3.37 NAEE140 pKa = 4.36 HH141 pKa = 6.73 NFEE144 pKa = 4.84 LDD146 pKa = 3.31 SSEE149 pKa = 4.44 GEE151 pKa = 4.36 VIGGQGGEE159 pKa = 4.61 DD160 pKa = 3.63 FGDD163 pKa = 4.0 DD164 pKa = 4.72 DD165 pKa = 4.19 EE166 pKa = 7.18 AEE168 pKa = 4.06
Molecular weight: 17.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.554
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.656
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.884
Patrickios 1.85
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A517Z2H3|A0A517Z2H3_9PLAN Fumarate hydratase class II OS=Maioricimonas rarisocia OX=2528026 GN=fumC PE=3 SV=1
MM1 pKa = 7.83 AKK3 pKa = 8.26 TQRR6 pKa = 11.84 KK7 pKa = 8.43 LKK9 pKa = 9.88 KK10 pKa = 9.26 ANHH13 pKa = 6.11 GKK15 pKa = 10.09 RR16 pKa = 11.84 PASAQARR23 pKa = 11.84 RR24 pKa = 11.84 SKK26 pKa = 10.37 RR27 pKa = 11.84 RR28 pKa = 11.84 HH29 pKa = 5.3 IKK31 pKa = 8.37 TT32 pKa = 3.26
Molecular weight: 3.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 10.994
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.574
Grimsley 12.764
Solomon 13.203
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.31
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.029
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5804
0
5804
2193700
29
8308
378.0
41.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.072 ± 0.034
1.152 ± 0.013
6.302 ± 0.026
6.757 ± 0.034
3.553 ± 0.018
7.974 ± 0.043
2.335 ± 0.018
4.538 ± 0.019
2.666 ± 0.027
9.955 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.081 ± 0.014
2.697 ± 0.022
5.666 ± 0.031
3.838 ± 0.022
7.681 ± 0.038
5.861 ± 0.022
5.579 ± 0.046
7.403 ± 0.031
1.557 ± 0.015
2.333 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here