Thrips-associated genomovirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykolovirus; Gemykolovirus echia1

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8YT69|A0A1P8YT69_9VIRU Replication associated protein OS=Thrips-associated genomovirus 4 OX=1941239 PE=4 SV=1
MM1 pKa = 7.1VRR3 pKa = 11.84RR4 pKa = 11.84FKK6 pKa = 10.58IDD8 pKa = 3.41DD9 pKa = 3.66EE10 pKa = 4.63QFFMLTYY17 pKa = 7.77PTTPDD22 pKa = 4.27DD23 pKa = 4.78FPIDD27 pKa = 3.99DD28 pKa = 4.82LVGILDD34 pKa = 4.16GLGCNYY40 pKa = 10.49RR41 pKa = 11.84IGRR44 pKa = 11.84EE45 pKa = 3.77LHH47 pKa = 6.79EE48 pKa = 5.17DD49 pKa = 3.94GKK51 pKa = 9.81PHH53 pKa = 5.72FHH55 pKa = 7.36AMVVFDD61 pKa = 4.8NPYY64 pKa = 9.56SDD66 pKa = 3.78RR67 pKa = 11.84DD68 pKa = 3.31GRR70 pKa = 11.84TTFRR74 pKa = 11.84VGGRR78 pKa = 11.84APNIRR83 pKa = 11.84MRR85 pKa = 11.84RR86 pKa = 11.84TKK88 pKa = 10.29PEE90 pKa = 4.07RR91 pKa = 11.84GWDD94 pKa = 3.64YY95 pKa = 11.11VGKK98 pKa = 9.63HH99 pKa = 5.96AGSKK103 pKa = 9.51DD104 pKa = 2.89GHH106 pKa = 6.41YY107 pKa = 10.36IVAEE111 pKa = 4.21KK112 pKa = 10.59GDD114 pKa = 3.86RR115 pKa = 11.84PGGDD119 pKa = 3.21GDD121 pKa = 4.68SNDD124 pKa = 3.77RR125 pKa = 11.84STNDD129 pKa = 2.62VWHH132 pKa = 6.8EE133 pKa = 4.01IILARR138 pKa = 11.84TRR140 pKa = 11.84EE141 pKa = 4.06EE142 pKa = 4.21FFDD145 pKa = 3.96LASRR149 pKa = 11.84LAPRR153 pKa = 11.84QLACSFTQLTSYY165 pKa = 11.32ADD167 pKa = 2.93WKK169 pKa = 9.97YY170 pKa = 10.64RR171 pKa = 11.84VVEE174 pKa = 4.19TPYY177 pKa = 11.01ASPEE181 pKa = 4.21GEE183 pKa = 3.73FEE185 pKa = 4.72VPIEE189 pKa = 3.8LQQWVNANLRR199 pKa = 11.84RR200 pKa = 11.84EE201 pKa = 3.98HH202 pKa = 6.65HH203 pKa = 6.12GRR205 pKa = 11.84PNGLVLFGATRR216 pKa = 11.84LGKK219 pKa = 7.65TVWARR224 pKa = 11.84SLGQHH229 pKa = 6.23YY230 pKa = 10.39YY231 pKa = 10.66CAGLWDD237 pKa = 4.47LSRR240 pKa = 11.84FDD242 pKa = 4.29EE243 pKa = 4.4SVEE246 pKa = 3.8YY247 pKa = 10.63AIFDD251 pKa = 4.02DD252 pKa = 4.08MVGGLRR258 pKa = 11.84AGYY261 pKa = 10.01FNYY264 pKa = 10.23KK265 pKa = 10.05DD266 pKa = 3.2WLGGQFEE273 pKa = 4.66FTVQDD278 pKa = 3.37KK279 pKa = 10.87YY280 pKa = 11.3KK281 pKa = 10.46HH282 pKa = 5.91KK283 pKa = 10.9KK284 pKa = 7.53QIKK287 pKa = 7.04WGKK290 pKa = 7.32PAIYY294 pKa = 9.74ICNQDD299 pKa = 3.51PRR301 pKa = 11.84TDD303 pKa = 3.21ITPMGKK309 pKa = 9.02NAIEE313 pKa = 3.99WDD315 pKa = 3.47WMEE318 pKa = 4.25EE319 pKa = 3.61NCVFYY324 pKa = 10.78EE325 pKa = 4.12CTEE328 pKa = 4.32TIFRR332 pKa = 11.84ASTT335 pKa = 3.11

Molecular weight: 38.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8YT69|A0A1P8YT69_9VIRU Replication associated protein OS=Thrips-associated genomovirus 4 OX=1941239 PE=4 SV=1
MM1 pKa = 7.54AYY3 pKa = 9.97SRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 9.66GSSRR12 pKa = 11.84AKK14 pKa = 9.64KK15 pKa = 4.61TTKK18 pKa = 9.96RR19 pKa = 11.84SGRR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84YY26 pKa = 4.92TAKK29 pKa = 9.55TRR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 9.34KK34 pKa = 10.73KK35 pKa = 8.69KK36 pKa = 8.14TYY38 pKa = 10.14RR39 pKa = 11.84KK40 pKa = 10.08ASMKK44 pKa = 10.18TILNKK49 pKa = 9.31TSRR52 pKa = 11.84KK53 pKa = 8.91KK54 pKa = 10.58RR55 pKa = 11.84NGMLTFANSTATGALATVSQAPLTIAGSPSGDD87 pKa = 3.24LRR89 pKa = 11.84GLIHH93 pKa = 7.06FRR95 pKa = 11.84PTSMDD100 pKa = 3.62LDD102 pKa = 3.71DD103 pKa = 4.91TVTNPNSIISTPSRR117 pKa = 11.84TASVCYY123 pKa = 9.54MKK125 pKa = 10.99GFAEE129 pKa = 4.13NVRR132 pKa = 11.84VEE134 pKa = 4.33TSTGNPWFHH143 pKa = 6.7RR144 pKa = 11.84RR145 pKa = 11.84ICITVKK151 pKa = 10.64HH152 pKa = 5.94PLFYY156 pKa = 11.08ALDD159 pKa = 3.77SRR161 pKa = 11.84DD162 pKa = 3.64TNGTDD167 pKa = 2.57RR168 pKa = 11.84LYY170 pKa = 10.88AARR173 pKa = 11.84GAIEE177 pKa = 4.47TSNGWQRR184 pKa = 11.84LASNVMTDD192 pKa = 3.63TLSNTYY198 pKa = 10.31GGWLSVMFKK207 pKa = 10.61GVQGVDD213 pKa = 2.49WDD215 pKa = 4.43DD216 pKa = 5.63FITAPIDD223 pKa = 3.49TNRR226 pKa = 11.84VDD228 pKa = 5.11LKK230 pKa = 11.0FDD232 pKa = 3.4KK233 pKa = 10.32TWVYY237 pKa = 10.84RR238 pKa = 11.84SGNEE242 pKa = 3.52RR243 pKa = 11.84GVLKK247 pKa = 9.03EE248 pKa = 4.08TKK250 pKa = 9.3LWHH253 pKa = 6.43PMNKK257 pKa = 9.35NLYY260 pKa = 9.28YY261 pKa = 10.79DD262 pKa = 4.61DD263 pKa = 5.72DD264 pKa = 4.52EE265 pKa = 6.6SGATEE270 pKa = 4.03EE271 pKa = 4.18TRR273 pKa = 11.84TWSVLDD279 pKa = 3.42KK280 pKa = 11.07RR281 pKa = 11.84GMGDD285 pKa = 2.98YY286 pKa = 10.63HH287 pKa = 8.66IFDD290 pKa = 5.17LFSQGSSGATTDD302 pKa = 3.61LLKK305 pKa = 10.47IRR307 pKa = 11.84FTSTMYY313 pKa = 9.45WHH315 pKa = 7.08EE316 pKa = 4.17KK317 pKa = 9.5

Molecular weight: 36.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

871

219

335

290.3

33.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.855 ± 0.194

1.148 ± 0.3

8.037 ± 0.598

5.166 ± 0.962

4.707 ± 0.532

8.496 ± 0.539

2.755 ± 0.396

4.248 ± 0.249

5.281 ± 0.911

6.315 ± 0.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.411 ± 0.315

3.904 ± 0.357

4.478 ± 0.792

2.296 ± 0.431

9.3 ± 0.641

5.741 ± 1.412

7.692 ± 1.656

5.166 ± 0.342

2.526 ± 0.283

4.478 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski