Corynebacterium phage SamW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samwavirus; Corynebacterium virus SamW

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385UJW9|A0A385UJW9_9CAUD Uncharacterized protein OS=Corynebacterium phage SamW OX=2301601 GN=47 PE=4 SV=1
MM1 pKa = 7.88TDD3 pKa = 3.23PDD5 pKa = 4.4FPDD8 pKa = 3.66SYY10 pKa = 11.23KK11 pKa = 10.64LLHH14 pKa = 6.18GAPYY18 pKa = 10.72DD19 pKa = 4.53DD20 pKa = 6.43DD21 pKa = 5.09PGPWPYY27 pKa = 10.86LAVIAFAAIAAVAILVASYY46 pKa = 10.82LL47 pKa = 3.74

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385UJW4|A0A385UJW4_9CAUD Uncharacterized protein OS=Corynebacterium phage SamW OX=2301601 GN=37 PE=4 SV=1
MM1 pKa = 8.05PEE3 pKa = 3.79TTNRR7 pKa = 11.84PTPHH11 pKa = 7.17ALDD14 pKa = 3.63SMRR17 pKa = 11.84GGAGATAPTGGSKK30 pKa = 10.93DD31 pKa = 3.81EE32 pKa = 4.21ITVSVQRR39 pKa = 11.84RR40 pKa = 11.84EE41 pKa = 3.63RR42 pKa = 11.84GGRR45 pKa = 11.84VRR47 pKa = 11.84WIGRR51 pKa = 11.84YY52 pKa = 9.18RR53 pKa = 11.84GLDD56 pKa = 3.02GKK58 pKa = 10.34EE59 pKa = 3.48RR60 pKa = 11.84SRR62 pKa = 11.84SFEE65 pKa = 3.7KK66 pKa = 10.72RR67 pKa = 11.84RR68 pKa = 11.84DD69 pKa = 3.41AVTWVAEE76 pKa = 4.44KK77 pKa = 10.75KK78 pKa = 9.81RR79 pKa = 11.84DD80 pKa = 3.58LRR82 pKa = 11.84RR83 pKa = 11.84GDD85 pKa = 3.58WIDD88 pKa = 3.39PAEE91 pKa = 4.02QKK93 pKa = 8.97ITIGEE98 pKa = 4.26LARR101 pKa = 11.84EE102 pKa = 4.07RR103 pKa = 11.84TMMARR108 pKa = 11.84TTNTKK113 pKa = 7.59ATRR116 pKa = 11.84RR117 pKa = 11.84FFEE120 pKa = 4.82ANLGPLEE127 pKa = 4.3GAAIGTVRR135 pKa = 11.84ASHH138 pKa = 5.1MRR140 pKa = 11.84EE141 pKa = 3.37WHH143 pKa = 5.06TMLRR147 pKa = 11.84NGRR150 pKa = 11.84PWQSGSLSPATIDD163 pKa = 3.34GLTVQMRR170 pKa = 11.84TLMKK174 pKa = 10.35QAVADD179 pKa = 4.09GLISRR184 pKa = 11.84SPAAGLEE191 pKa = 4.11RR192 pKa = 11.84VGRR195 pKa = 11.84DD196 pKa = 3.14VVVIPRR202 pKa = 11.84DD203 pKa = 3.26IPTPAEE209 pKa = 3.78VGALIRR215 pKa = 11.84AFEE218 pKa = 4.07TGIRR222 pKa = 11.84APEE225 pKa = 4.02PLVPQYY231 pKa = 10.97RR232 pKa = 11.84RR233 pKa = 11.84DD234 pKa = 3.42GRR236 pKa = 11.84RR237 pKa = 11.84KK238 pKa = 8.85PRR240 pKa = 11.84GWLLKK245 pKa = 8.91PAPWMARR252 pKa = 11.84AVRR255 pKa = 11.84LAIATGMRR263 pKa = 11.84PSEE266 pKa = 4.07VAGMRR271 pKa = 11.84VRR273 pKa = 11.84SLDD276 pKa = 3.44QLTGSVDD283 pKa = 3.69VVEE286 pKa = 4.36QAARR290 pKa = 11.84HH291 pKa = 4.38STEE294 pKa = 3.8TVEE297 pKa = 5.32LKK299 pKa = 10.07TEE301 pKa = 4.38ASRR304 pKa = 11.84RR305 pKa = 11.84WLPVDD310 pKa = 3.92AEE312 pKa = 4.42TLHH315 pKa = 7.18VLVDD319 pKa = 3.7WAQDD323 pKa = 3.18RR324 pKa = 11.84DD325 pKa = 3.94LGPDD329 pKa = 2.8ARR331 pKa = 11.84LFHH334 pKa = 6.45VRR336 pKa = 11.84GGNPATAEE344 pKa = 4.37AIGRR348 pKa = 11.84LMRR351 pKa = 11.84SAVDD355 pKa = 3.19AGVWRR360 pKa = 11.84GGMTFHH366 pKa = 7.62SLRR369 pKa = 11.84HH370 pKa = 4.92FHH372 pKa = 7.06ASALIAAGLDD382 pKa = 3.32VAVVSRR388 pKa = 11.84RR389 pKa = 11.84LGHH392 pKa = 6.35EE393 pKa = 3.99NMSTTLEE400 pKa = 4.57VYY402 pKa = 8.49THH404 pKa = 7.03LWPGSEE410 pKa = 3.9EE411 pKa = 4.31RR412 pKa = 11.84EE413 pKa = 4.0RR414 pKa = 11.84EE415 pKa = 3.8AATALVRR422 pKa = 11.84DD423 pKa = 3.83VCGTGGRR430 pKa = 11.84GEE432 pKa = 4.48GSDD435 pKa = 3.5SGSVQVSS442 pKa = 3.17

Molecular weight: 48.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13852

45

1796

230.9

25.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.836 ± 0.672

0.599 ± 0.103

6.995 ± 0.355

6.18 ± 0.252

2.498 ± 0.172

8.757 ± 0.469

2.296 ± 0.254

4.808 ± 0.188

3.285 ± 0.203

7.826 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.098

2.418 ± 0.168

5.754 ± 0.356

3.451 ± 0.156

7.031 ± 0.426

4.866 ± 0.339

6.627 ± 0.298

6.995 ± 0.235

2.216 ± 0.139

2.115 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski