Amino acid dipepetide frequency for Corynebacterium phage SamW

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.782AlaAla: 19.782 ± 2.474
0.505AlaCys: 0.505 ± 0.21
9.386AlaAsp: 9.386 ± 0.98
9.674AlaGlu: 9.674 ± 0.946
2.743AlaPhe: 2.743 ± 0.59
11.118AlaGly: 11.118 ± 1.195
2.094AlaHis: 2.094 ± 0.421
6.498AlaIle: 6.498 ± 0.68
3.826AlaLys: 3.826 ± 0.664
10.252AlaLeu: 10.252 ± 0.819
3.538AlaMet: 3.538 ± 0.593
2.743AlaAsn: 2.743 ± 0.61
7.22AlaPro: 7.22 ± 0.872
4.837AlaGln: 4.837 ± 0.8
8.086AlaArg: 8.086 ± 0.771
4.909AlaSer: 4.909 ± 0.696
7.003AlaThr: 7.003 ± 0.544
8.88AlaVal: 8.88 ± 0.867
2.382AlaTrp: 2.382 ± 0.477
2.888AlaTyr: 2.888 ± 0.506
0.0AlaXaa: 0.0 ± 0.0
Cys
0.505CysAla: 0.505 ± 0.23
0.072CysCys: 0.072 ± 0.098
0.217CysAsp: 0.217 ± 0.136
0.361CysGlu: 0.361 ± 0.181
0.144CysPhe: 0.144 ± 0.106
0.578CysGly: 0.578 ± 0.195
0.072CysHis: 0.072 ± 0.091
0.217CysIle: 0.217 ± 0.122
0.289CysLys: 0.289 ± 0.133
0.578CysLeu: 0.578 ± 0.219
0.0CysMet: 0.0 ± 0.0
0.361CysAsn: 0.361 ± 0.175
0.217CysPro: 0.217 ± 0.14
0.289CysGln: 0.289 ± 0.169
0.505CysArg: 0.505 ± 0.171
0.433CysSer: 0.433 ± 0.169
0.505CysThr: 0.505 ± 0.225
0.289CysVal: 0.289 ± 0.165
0.217CysTrp: 0.217 ± 0.139
0.144CysTyr: 0.144 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
10.18AspAla: 10.18 ± 0.8
0.433AspCys: 0.433 ± 0.192
5.415AspAsp: 5.415 ± 0.833
4.909AspGlu: 4.909 ± 0.495
1.588AspPhe: 1.588 ± 0.349
7.869AspGly: 7.869 ± 0.83
1.516AspHis: 1.516 ± 0.409
2.382AspIle: 2.382 ± 0.562
1.588AspLys: 1.588 ± 0.545
5.631AspLeu: 5.631 ± 0.604
1.372AspMet: 1.372 ± 0.289
1.3AspAsn: 1.3 ± 0.294
3.971AspPro: 3.971 ± 0.721
3.104AspGln: 3.104 ± 0.564
3.826AspArg: 3.826 ± 0.554
2.888AspSer: 2.888 ± 0.509
3.826AspThr: 3.826 ± 0.842
4.765AspVal: 4.765 ± 0.634
1.949AspTrp: 1.949 ± 0.338
1.444AspTyr: 1.444 ± 0.34
0.0AspXaa: 0.0 ± 0.0
Glu
6.426GluAla: 6.426 ± 0.554
0.361GluCys: 0.361 ± 0.173
2.888GluAsp: 2.888 ± 0.443
3.682GluGlu: 3.682 ± 0.591
1.805GluPhe: 1.805 ± 0.351
3.538GluGly: 3.538 ± 0.481
1.733GluHis: 1.733 ± 0.385
3.393GluIle: 3.393 ± 0.53
2.455GluLys: 2.455 ± 0.4
5.776GluLeu: 5.776 ± 0.699
1.3GluMet: 1.3 ± 0.295
1.516GluAsn: 1.516 ± 0.302
4.332GluPro: 4.332 ± 0.774
3.465GluGln: 3.465 ± 0.375
4.404GluArg: 4.404 ± 0.635
3.249GluSer: 3.249 ± 0.432
3.538GluThr: 3.538 ± 0.495
4.332GluVal: 4.332 ± 0.547
1.372GluTrp: 1.372 ± 0.341
2.238GluTyr: 2.238 ± 0.442
0.0GluXaa: 0.0 ± 0.0
Phe
2.094PheAla: 2.094 ± 0.356
0.289PheCys: 0.289 ± 0.135
2.31PheAsp: 2.31 ± 0.383
1.661PheGlu: 1.661 ± 0.452
1.011PhePhe: 1.011 ± 0.403
3.249PheGly: 3.249 ± 0.478
0.65PheHis: 0.65 ± 0.245
1.372PheIle: 1.372 ± 0.267
0.866PheLys: 0.866 ± 0.215
2.022PheLeu: 2.022 ± 0.422
0.578PheMet: 0.578 ± 0.163
0.361PheAsn: 0.361 ± 0.144
0.939PhePro: 0.939 ± 0.272
0.722PheGln: 0.722 ± 0.201
1.444PheArg: 1.444 ± 0.361
1.3PheSer: 1.3 ± 0.433
1.661PheThr: 1.661 ± 0.315
1.372PheVal: 1.372 ± 0.314
0.65PheTrp: 0.65 ± 0.209
0.433PheTyr: 0.433 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
8.736GlyAla: 8.736 ± 1.094
0.361GlyCys: 0.361 ± 0.182
5.487GlyAsp: 5.487 ± 0.567
5.343GlyGlu: 5.343 ± 0.63
2.888GlyPhe: 2.888 ± 0.404
7.942GlyGly: 7.942 ± 1.033
2.816GlyHis: 2.816 ± 0.536
3.899GlyIle: 3.899 ± 0.744
4.26GlyLys: 4.26 ± 0.642
6.137GlyLeu: 6.137 ± 0.594
3.104GlyMet: 3.104 ± 0.483
2.527GlyAsn: 2.527 ± 0.493
4.26GlyPro: 4.26 ± 0.537
2.743GlyGln: 2.743 ± 0.434
6.353GlyArg: 6.353 ± 0.662
4.765GlySer: 4.765 ± 0.849
5.487GlyThr: 5.487 ± 0.58
6.498GlyVal: 6.498 ± 0.859
2.238GlyTrp: 2.238 ± 0.342
1.733GlyTyr: 1.733 ± 0.251
0.0GlyXaa: 0.0 ± 0.0
His
2.382HisAla: 2.382 ± 0.43
0.289HisCys: 0.289 ± 0.152
1.444HisAsp: 1.444 ± 0.387
1.083HisGlu: 1.083 ± 0.267
0.578HisPhe: 0.578 ± 0.24
1.877HisGly: 1.877 ± 0.428
0.939HisHis: 0.939 ± 0.343
1.661HisIle: 1.661 ± 0.453
0.505HisLys: 0.505 ± 0.205
2.166HisLeu: 2.166 ± 0.567
0.289HisMet: 0.289 ± 0.153
0.361HisAsn: 0.361 ± 0.158
1.372HisPro: 1.372 ± 0.343
1.083HisGln: 1.083 ± 0.275
1.155HisArg: 1.155 ± 0.3
0.939HisSer: 0.939 ± 0.247
1.372HisThr: 1.372 ± 0.298
2.31HisVal: 2.31 ± 0.403
0.505HisTrp: 0.505 ± 0.286
0.65HisTyr: 0.65 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
6.931IleAla: 6.931 ± 0.675
0.217IleCys: 0.217 ± 0.166
4.693IleAsp: 4.693 ± 0.78
2.382IleGlu: 2.382 ± 0.424
1.011IlePhe: 1.011 ± 0.258
4.837IleGly: 4.837 ± 0.929
1.227IleHis: 1.227 ± 0.306
2.743IleIle: 2.743 ± 0.413
1.011IleLys: 1.011 ± 0.266
2.816IleLeu: 2.816 ± 0.479
0.65IleMet: 0.65 ± 0.185
1.227IleAsn: 1.227 ± 0.277
2.96IlePro: 2.96 ± 0.611
1.444IleGln: 1.444 ± 0.318
2.96IleArg: 2.96 ± 0.483
2.671IleSer: 2.671 ± 0.524
3.754IleThr: 3.754 ± 0.595
3.177IleVal: 3.177 ± 0.402
0.289IleTrp: 0.289 ± 0.17
1.083IleTyr: 1.083 ± 0.344
0.0IleXaa: 0.0 ± 0.0
Lys
4.332LysAla: 4.332 ± 0.924
0.0LysCys: 0.0 ± 0.0
1.805LysAsp: 1.805 ± 0.382
1.3LysGlu: 1.3 ± 0.284
0.866LysPhe: 0.866 ± 0.292
2.238LysGly: 2.238 ± 0.414
0.722LysHis: 0.722 ± 0.212
1.588LysIle: 1.588 ± 0.356
1.227LysLys: 1.227 ± 0.347
3.682LysLeu: 3.682 ± 0.505
0.794LysMet: 0.794 ± 0.316
0.505LysAsn: 0.505 ± 0.192
1.949LysPro: 1.949 ± 0.333
1.877LysGln: 1.877 ± 0.434
1.877LysArg: 1.877 ± 0.302
2.094LysSer: 2.094 ± 0.45
2.382LysThr: 2.382 ± 0.397
1.805LysVal: 1.805 ± 0.407
0.866LysTrp: 0.866 ± 0.23
0.939LysTyr: 0.939 ± 0.191
0.0LysXaa: 0.0 ± 0.0
Leu
10.035LeuAla: 10.035 ± 0.965
0.505LeuCys: 0.505 ± 0.244
6.57LeuAsp: 6.57 ± 0.87
3.971LeuGlu: 3.971 ± 0.509
1.444LeuPhe: 1.444 ± 0.341
6.931LeuGly: 6.931 ± 0.736
1.949LeuHis: 1.949 ± 0.475
3.682LeuIle: 3.682 ± 0.438
2.527LeuLys: 2.527 ± 0.483
4.982LeuLeu: 4.982 ± 0.68
1.588LeuMet: 1.588 ± 0.289
2.022LeuAsn: 2.022 ± 0.33
5.631LeuPro: 5.631 ± 0.768
2.166LeuGln: 2.166 ± 0.357
6.57LeuArg: 6.57 ± 0.776
4.404LeuSer: 4.404 ± 0.614
5.198LeuThr: 5.198 ± 0.633
4.621LeuVal: 4.621 ± 0.527
1.661LeuTrp: 1.661 ± 0.363
1.805LeuTyr: 1.805 ± 0.432
0.0LeuXaa: 0.0 ± 0.0
Met
2.816MetAla: 2.816 ± 0.518
0.072MetCys: 0.072 ± 0.091
1.661MetAsp: 1.661 ± 0.276
1.805MetGlu: 1.805 ± 0.463
0.361MetPhe: 0.361 ± 0.154
2.238MetGly: 2.238 ± 0.63
0.289MetHis: 0.289 ± 0.166
0.722MetIle: 0.722 ± 0.25
1.155MetLys: 1.155 ± 0.305
1.011MetLeu: 1.011 ± 0.276
0.361MetMet: 0.361 ± 0.154
0.722MetAsn: 0.722 ± 0.211
1.733MetPro: 1.733 ± 0.302
0.65MetGln: 0.65 ± 0.182
1.805MetArg: 1.805 ± 0.54
1.733MetSer: 1.733 ± 0.332
3.61MetThr: 3.61 ± 0.702
1.155MetVal: 1.155 ± 0.261
0.289MetTrp: 0.289 ± 0.172
0.289MetTyr: 0.289 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
3.61AsnAla: 3.61 ± 0.482
0.072AsnCys: 0.072 ± 0.082
1.083AsnAsp: 1.083 ± 0.262
1.011AsnGlu: 1.011 ± 0.249
0.505AsnPhe: 0.505 ± 0.207
2.888AsnGly: 2.888 ± 0.62
0.65AsnHis: 0.65 ± 0.237
1.083AsnIle: 1.083 ± 0.224
0.65AsnLys: 0.65 ± 0.236
2.671AsnLeu: 2.671 ± 0.399
0.361AsnMet: 0.361 ± 0.128
0.505AsnAsn: 0.505 ± 0.184
2.238AsnPro: 2.238 ± 0.418
0.433AsnGln: 0.433 ± 0.195
2.094AsnArg: 2.094 ± 0.37
0.866AsnSer: 0.866 ± 0.236
1.3AsnThr: 1.3 ± 0.271
1.516AsnVal: 1.516 ± 0.321
0.072AsnTrp: 0.072 ± 0.076
0.578AsnTyr: 0.578 ± 0.267
0.0AsnXaa: 0.0 ± 0.0
Pro
7.003ProAla: 7.003 ± 0.677
0.217ProCys: 0.217 ± 0.165
5.126ProAsp: 5.126 ± 0.644
4.476ProGlu: 4.476 ± 0.682
1.661ProPhe: 1.661 ± 0.32
5.487ProGly: 5.487 ± 0.871
0.866ProHis: 0.866 ± 0.332
2.455ProIle: 2.455 ± 0.455
2.094ProLys: 2.094 ± 0.516
3.61ProLeu: 3.61 ± 0.485
1.877ProMet: 1.877 ± 0.48
1.227ProAsn: 1.227 ± 0.31
3.393ProPro: 3.393 ± 0.794
1.444ProGln: 1.444 ± 0.371
3.321ProArg: 3.321 ± 0.57
2.671ProSer: 2.671 ± 0.521
3.826ProThr: 3.826 ± 0.535
4.548ProVal: 4.548 ± 0.494
1.444ProTrp: 1.444 ± 0.398
0.794ProTyr: 0.794 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
5.126GlnAla: 5.126 ± 0.564
0.217GlnCys: 0.217 ± 0.123
1.083GlnAsp: 1.083 ± 0.286
1.805GlnGlu: 1.805 ± 0.402
0.794GlnPhe: 0.794 ± 0.309
2.671GlnGly: 2.671 ± 0.475
0.578GlnHis: 0.578 ± 0.206
1.372GlnIle: 1.372 ± 0.287
1.444GlnLys: 1.444 ± 0.284
3.465GlnLeu: 3.465 ± 0.418
1.083GlnMet: 1.083 ± 0.289
0.722GlnAsn: 0.722 ± 0.298
1.3GlnPro: 1.3 ± 0.321
1.516GlnGln: 1.516 ± 0.303
2.527GlnArg: 2.527 ± 0.4
1.083GlnSer: 1.083 ± 0.281
1.805GlnThr: 1.805 ± 0.295
3.826GlnVal: 3.826 ± 0.453
1.3GlnTrp: 1.3 ± 0.288
0.722GlnTyr: 0.722 ± 0.254
0.0GlnXaa: 0.0 ± 0.0
Arg
7.797ArgAla: 7.797 ± 0.833
0.722ArgCys: 0.722 ± 0.288
6.065ArgAsp: 6.065 ± 0.814
4.115ArgGlu: 4.115 ± 0.448
2.094ArgPhe: 2.094 ± 0.312
4.693ArgGly: 4.693 ± 0.721
1.588ArgHis: 1.588 ± 0.447
3.971ArgIle: 3.971 ± 0.552
2.743ArgLys: 2.743 ± 0.344
5.559ArgLeu: 5.559 ± 0.895
2.599ArgMet: 2.599 ± 0.407
1.733ArgAsn: 1.733 ± 0.337
3.249ArgPro: 3.249 ± 0.594
2.166ArgGln: 2.166 ± 0.528
6.57ArgArg: 6.57 ± 0.794
3.032ArgSer: 3.032 ± 0.48
4.043ArgThr: 4.043 ± 0.461
4.404ArgVal: 4.404 ± 0.471
2.022ArgTrp: 2.022 ± 0.418
1.155ArgTyr: 1.155 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
5.631SerAla: 5.631 ± 0.746
0.433SerCys: 0.433 ± 0.26
2.599SerAsp: 2.599 ± 0.537
2.455SerGlu: 2.455 ± 0.557
1.588SerPhe: 1.588 ± 0.376
5.198SerGly: 5.198 ± 1.046
1.155SerHis: 1.155 ± 0.425
1.877SerIle: 1.877 ± 0.371
1.083SerLys: 1.083 ± 0.24
3.754SerLeu: 3.754 ± 0.722
1.155SerMet: 1.155 ± 0.294
1.083SerAsn: 1.083 ± 0.277
2.238SerPro: 2.238 ± 0.476
1.661SerGln: 1.661 ± 0.318
3.971SerArg: 3.971 ± 0.574
2.599SerSer: 2.599 ± 0.519
3.971SerThr: 3.971 ± 0.611
3.971SerVal: 3.971 ± 0.585
1.083SerTrp: 1.083 ± 0.233
1.155SerTyr: 1.155 ± 0.298
0.0SerXaa: 0.0 ± 0.0
Thr
8.375ThrAla: 8.375 ± 0.763
0.217ThrCys: 0.217 ± 0.165
4.043ThrAsp: 4.043 ± 0.687
3.61ThrGlu: 3.61 ± 0.364
1.3ThrPhe: 1.3 ± 0.278
5.776ThrGly: 5.776 ± 0.521
1.372ThrHis: 1.372 ± 0.326
4.043ThrIle: 4.043 ± 0.907
2.382ThrLys: 2.382 ± 0.341
5.631ThrLeu: 5.631 ± 0.623
1.949ThrMet: 1.949 ± 0.426
2.094ThrAsn: 2.094 ± 0.374
4.476ThrPro: 4.476 ± 0.698
1.516ThrGln: 1.516 ± 0.344
3.754ThrArg: 3.754 ± 0.671
2.382ThrSer: 2.382 ± 0.703
5.487ThrThr: 5.487 ± 0.866
5.126ThrVal: 5.126 ± 0.79
1.227ThrTrp: 1.227 ± 0.321
1.516ThrTyr: 1.516 ± 0.343
0.0ThrXaa: 0.0 ± 0.0
Val
10.83ValAla: 10.83 ± 0.916
0.433ValCys: 0.433 ± 0.185
5.054ValAsp: 5.054 ± 0.785
5.343ValGlu: 5.343 ± 0.609
1.444ValPhe: 1.444 ± 0.361
5.487ValGly: 5.487 ± 0.74
1.516ValHis: 1.516 ± 0.363
2.96ValIle: 2.96 ± 0.377
2.022ValLys: 2.022 ± 0.434
5.126ValLeu: 5.126 ± 0.684
0.866ValMet: 0.866 ± 0.328
1.949ValAsn: 1.949 ± 0.425
3.538ValPro: 3.538 ± 0.468
1.733ValGln: 1.733 ± 0.351
4.909ValArg: 4.909 ± 0.634
4.043ValSer: 4.043 ± 0.583
4.909ValThr: 4.909 ± 0.645
4.693ValVal: 4.693 ± 0.662
1.805ValTrp: 1.805 ± 0.296
1.3ValTyr: 1.3 ± 0.328
0.0ValXaa: 0.0 ± 0.0
Trp
3.393TrpAla: 3.393 ± 0.564
0.361TrpCys: 0.361 ± 0.169
1.516TrpAsp: 1.516 ± 0.319
1.3TrpGlu: 1.3 ± 0.304
0.722TrpPhe: 0.722 ± 0.233
1.3TrpGly: 1.3 ± 0.353
0.505TrpHis: 0.505 ± 0.186
1.083TrpIle: 1.083 ± 0.287
0.289TrpLys: 0.289 ± 0.144
2.022TrpLeu: 2.022 ± 0.397
0.65TrpMet: 0.65 ± 0.234
0.722TrpAsn: 0.722 ± 0.242
1.444TrpPro: 1.444 ± 0.38
0.794TrpGln: 0.794 ± 0.191
2.022TrpArg: 2.022 ± 0.258
1.155TrpSer: 1.155 ± 0.309
1.011TrpThr: 1.011 ± 0.388
1.227TrpVal: 1.227 ± 0.258
0.505TrpTrp: 0.505 ± 0.165
0.144TrpTyr: 0.144 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.382TyrAla: 2.382 ± 0.369
0.217TyrCys: 0.217 ± 0.159
1.516TyrAsp: 1.516 ± 0.286
1.516TyrGlu: 1.516 ± 0.335
0.433TyrPhe: 0.433 ± 0.197
1.661TyrGly: 1.661 ± 0.35
0.722TyrHis: 0.722 ± 0.284
1.083TyrIle: 1.083 ± 0.338
0.433TyrLys: 0.433 ± 0.239
1.372TyrLeu: 1.372 ± 0.376
0.289TyrMet: 0.289 ± 0.15
0.505TyrAsn: 0.505 ± 0.172
1.083TyrPro: 1.083 ± 0.288
0.866TyrGln: 0.866 ± 0.281
2.166TyrArg: 2.166 ± 0.349
1.444TyrSer: 1.444 ± 0.38
1.588TyrThr: 1.588 ± 0.334
1.444TyrVal: 1.444 ± 0.29
0.289TyrTrp: 0.289 ± 0.177
0.144TyrTyr: 0.144 ± 0.102
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (13852 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski