Erythrobacter sp. SD-21

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; unclassified Erythrobacter

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2938 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5P742|A5P742_9SPHN Transcription elongation factor GreB OS=Erythrobacter sp. SD-21 OX=161528 GN=greB PE=3 SV=1
MM1 pKa = 7.28AQDD4 pKa = 3.83SDD6 pKa = 3.88NFDD9 pKa = 3.54GFRR12 pKa = 11.84IEE14 pKa = 5.27ALAGYY19 pKa = 9.22DD20 pKa = 3.47VSKK23 pKa = 11.15AGSTSDD29 pKa = 3.73NDD31 pKa = 3.63TNANDD36 pKa = 3.88DD37 pKa = 3.52QSIDD41 pKa = 3.27GLAYY45 pKa = 10.21GVGAGYY51 pKa = 10.72DD52 pKa = 3.66FDD54 pKa = 6.0AGGVVLGLEE63 pKa = 4.11AEE65 pKa = 4.72YY66 pKa = 11.05VGSTAEE72 pKa = 4.05TEE74 pKa = 3.9YY75 pKa = 11.66DD76 pKa = 3.13MGDD79 pKa = 3.48FEE81 pKa = 7.0DD82 pKa = 3.74IGVGNVEE89 pKa = 4.36TGRR92 pKa = 11.84DD93 pKa = 3.62LYY95 pKa = 11.26LGARR99 pKa = 11.84VGVKK103 pKa = 10.59ANDD106 pKa = 3.85DD107 pKa = 3.87LLVYY111 pKa = 10.91AKK113 pKa = 10.47GGYY116 pKa = 8.23TNATYY121 pKa = 11.09NFRR124 pKa = 11.84NNDD127 pKa = 3.02GTNEE131 pKa = 4.05YY132 pKa = 10.39EE133 pKa = 4.35VDD135 pKa = 3.8LDD137 pKa = 3.7TDD139 pKa = 3.96GFRR142 pKa = 11.84VGGGLEE148 pKa = 4.04YY149 pKa = 10.84ALSGNTFAKK158 pKa = 10.36VEE160 pKa = 4.03YY161 pKa = 9.72RR162 pKa = 11.84YY163 pKa = 11.06SNYY166 pKa = 10.23SDD168 pKa = 4.95AEE170 pKa = 4.09LDD172 pKa = 4.04FEE174 pKa = 6.04GDD176 pKa = 3.7APDD179 pKa = 3.79VAVPDD184 pKa = 3.7IDD186 pKa = 4.79LDD188 pKa = 3.71RR189 pKa = 11.84HH190 pKa = 4.31QVMAGFGMRR199 pKa = 11.84FF200 pKa = 3.14

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5PCQ3|A5PCQ3_9SPHN Ribonuclease P protein component OS=Erythrobacter sp. SD-21 OX=161528 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 8.0VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.44NLCAA44 pKa = 4.54

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2938

0

2938

896752

14

2532

305.2

33.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.063 ± 0.056

0.816 ± 0.017

6.323 ± 0.048

6.874 ± 0.052

3.673 ± 0.03

8.834 ± 0.065

1.955 ± 0.024

5.011 ± 0.031

3.44 ± 0.042

9.713 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.022

2.666 ± 0.031

4.889 ± 0.037

3.152 ± 0.026

6.896 ± 0.047

5.375 ± 0.036

5.184 ± 0.032

6.937 ± 0.032

1.417 ± 0.018

2.248 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski