Amino acid dipepetide frequency for Erythrobacter sp. SD-21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.05AlaAla: 15.05 ± 0.178	1.074AlaCys: 1.074 ± 0.038	6.954AlaAsp: 6.954 ± 0.09	8.511AlaGlu: 8.511 ± 0.106	4.067AlaPhe: 4.067 ± 0.076	10.131AlaGly: 10.131 ± 0.108	2.187AlaHis: 2.187 ± 0.051	6.579AlaIle: 6.579 ± 0.098	4.587AlaLys: 4.587 ± 0.089	12.723AlaLeu: 12.723 ± 0.163	3.711AlaMet: 3.711 ± 0.073	3.188AlaAsn: 3.188 ± 0.063	5.311AlaPro: 5.311 ± 0.096	4.294AlaGln: 4.294 ± 0.083	8.506AlaArg: 8.506 ± 0.126	6.547AlaSer: 6.547 ± 0.105	5.794AlaThr: 5.794 ± 0.099	7.505AlaVal: 7.505 ± 0.098	1.449AlaTrp: 1.449 ± 0.05	2.458AlaTyr: 2.458 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.99CysAla: 0.99 ± 0.035	0.093CysCys: 0.093 ± 0.01	0.583CysAsp: 0.583 ± 0.03	0.581CysGlu: 0.581 ± 0.027	0.293CysPhe: 0.293 ± 0.018	0.843CysGly: 0.843 ± 0.035	0.263CysHis: 0.263 ± 0.047	0.364CysIle: 0.364 ± 0.02	0.211CysLys: 0.211 ± 0.015	0.697CysLeu: 0.697 ± 0.028	0.145CysMet: 0.145 ± 0.014	0.234CysAsn: 0.234 ± 0.016	0.401CysPro: 0.401 ± 0.023	0.229CysGln: 0.229 ± 0.018	0.543CysArg: 0.543 ± 0.025	0.475CysSer: 0.475 ± 0.024	0.405CysThr: 0.405 ± 0.02	0.525CysVal: 0.525 ± 0.023	0.113CysTrp: 0.113 ± 0.01	0.175CysTyr: 0.175 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.222AspAla: 7.222 ± 0.095	0.558AspCys: 0.558 ± 0.033	3.748AspAsp: 3.748 ± 0.12	4.646AspGlu: 4.646 ± 0.074	2.461AspPhe: 2.461 ± 0.058	5.852AspGly: 5.852 ± 0.12	1.353AspHis: 1.353 ± 0.04	3.184AspIle: 3.184 ± 0.069	2.084AspLys: 2.084 ± 0.051	6.032AspLeu: 6.032 ± 0.089	1.637AspMet: 1.637 ± 0.042	1.647AspAsn: 1.647 ± 0.055	3.603AspPro: 3.603 ± 0.065	1.896AspGln: 1.896 ± 0.049	4.671AspArg: 4.671 ± 0.068	2.603AspSer: 2.603 ± 0.053	3.21AspThr: 3.21 ± 0.089	3.945AspVal: 3.945 ± 0.074	1.208AspTrp: 1.208 ± 0.034	1.669AspTyr: 1.669 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	8.974GluAla: 8.974 ± 0.128	0.436GluCys: 0.436 ± 0.021	4.238GluAsp: 4.238 ± 0.08	5.057GluGlu: 5.057 ± 0.105	2.12GluPhe: 2.12 ± 0.052	5.992GluGly: 5.992 ± 0.089	1.349GluHis: 1.349 ± 0.039	3.563GluIle: 3.563 ± 0.07	2.776GluLys: 2.776 ± 0.066	6.15GluLeu: 6.15 ± 0.096	1.825GluMet: 1.825 ± 0.048	1.966GluAsn: 1.966 ± 0.042	2.997GluPro: 2.997 ± 0.071	2.51GluGln: 2.51 ± 0.057	5.543GluArg: 5.543 ± 0.103	2.676GluSer: 2.676 ± 0.052	3.709GluThr: 3.709 ± 0.071	4.494GluVal: 4.494 ± 0.083	1.029GluTrp: 1.029 ± 0.036	1.34GluTyr: 1.34 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	4.899PheAla: 4.899 ± 0.086	0.346PheCys: 0.346 ± 0.019	3.009PheAsp: 3.009 ± 0.065	2.507PheGlu: 2.507 ± 0.056	1.401PhePhe: 1.401 ± 0.043	3.74PheGly: 3.74 ± 0.08	0.688PheHis: 0.688 ± 0.03	1.481PheIle: 1.481 ± 0.04	0.94PheLys: 0.94 ± 0.037	3.193PheLeu: 3.193 ± 0.078	0.786PheMet: 0.786 ± 0.031	1.024PheAsn: 1.024 ± 0.037	1.456PhePro: 1.456 ± 0.046	0.904PheGln: 0.904 ± 0.028	2.091PheArg: 2.091 ± 0.055	2.086PheSer: 2.086 ± 0.05	1.999PheThr: 1.999 ± 0.051	2.682PheVal: 2.682 ± 0.059	0.55PheTrp: 0.55 ± 0.027	0.949PheTyr: 0.949 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	9.174GlyAla: 9.174 ± 0.121	0.771GlyCys: 0.771 ± 0.031	5.495GlyAsp: 5.495 ± 0.141	6.411GlyGlu: 6.411 ± 0.087	3.677GlyPhe: 3.677 ± 0.071	8.076GlyGly: 8.076 ± 0.208	1.799GlyHis: 1.799 ± 0.044	4.401GlyIle: 4.401 ± 0.072	3.662GlyLys: 3.662 ± 0.087	8.346GlyLeu: 8.346 ± 0.108	2.435GlyMet: 2.435 ± 0.058	2.724GlyAsn: 2.724 ± 0.116	3.381GlyPro: 3.381 ± 0.061	2.936GlyGln: 2.936 ± 0.056	5.617GlyArg: 5.617 ± 0.081	5.081GlySer: 5.081 ± 0.084	4.717GlyThr: 4.717 ± 0.093	5.817GlyVal: 5.817 ± 0.096	1.547GlyTrp: 1.547 ± 0.036	2.269GlyTyr: 2.269 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	2.193HisAla: 2.193 ± 0.057	0.244HisCys: 0.244 ± 0.018	1.155HisAsp: 1.155 ± 0.039	1.265HisGlu: 1.265 ± 0.044	0.881HisPhe: 0.881 ± 0.032	1.896HisGly: 1.896 ± 0.051	0.531HisHis: 0.531 ± 0.027	0.934HisIle: 0.934 ± 0.037	0.564HisLys: 0.564 ± 0.026	1.753HisLeu: 1.753 ± 0.049	0.435HisMet: 0.435 ± 0.02	0.466HisAsn: 0.466 ± 0.029	1.179HisPro: 1.179 ± 0.039	0.535HisGln: 0.535 ± 0.026	1.445HisArg: 1.445 ± 0.042	0.997HisSer: 0.997 ± 0.034	0.816HisThr: 0.816 ± 0.028	1.386HisVal: 1.386 ± 0.047	0.348HisTrp: 0.348 ± 0.02	0.526HisTyr: 0.526 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.224IleAla: 7.224 ± 0.095	0.466IleCys: 0.466 ± 0.021	3.867IleAsp: 3.867 ± 0.068	4.137IleGlu: 4.137 ± 0.068	1.694IlePhe: 1.694 ± 0.051	4.897IleGly: 4.897 ± 0.082	0.91IleHis: 0.91 ± 0.03	2.075IleIle: 2.075 ± 0.066	1.308IleLys: 1.308 ± 0.044	4.108IleLeu: 4.108 ± 0.071	0.956IleMet: 0.956 ± 0.033	1.266IleAsn: 1.266 ± 0.042	2.21IlePro: 2.21 ± 0.049	1.137IleGln: 1.137 ± 0.032	2.987IleArg: 2.987 ± 0.056	2.714IleSer: 2.714 ± 0.055	2.58IleThr: 2.58 ± 0.067	3.827IleVal: 3.827 ± 0.071	0.632IleTrp: 0.632 ± 0.028	1.11IleTyr: 1.11 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.346LysAla: 4.346 ± 0.089	0.183LysCys: 0.183 ± 0.014	1.958LysAsp: 1.958 ± 0.053	1.843LysGlu: 1.843 ± 0.058	1.036LysPhe: 1.036 ± 0.036	2.936LysGly: 2.936 ± 0.066	0.701LysHis: 0.701 ± 0.033	1.606LysIle: 1.606 ± 0.046	1.612LysLys: 1.612 ± 0.068	3.508LysLeu: 3.508 ± 0.065	0.84LysMet: 0.84 ± 0.028	0.872LysAsn: 0.872 ± 0.031	2.086LysPro: 2.086 ± 0.05	1.121LysGln: 1.121 ± 0.036	2.633LysArg: 2.633 ± 0.06	1.774LysSer: 1.774 ± 0.051	1.798LysThr: 1.798 ± 0.051	2.44LysVal: 2.44 ± 0.061	0.436LysTrp: 0.436 ± 0.022	0.675LysTyr: 0.675 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	13.476LeuAla: 13.476 ± 0.183	0.755LeuCys: 0.755 ± 0.031	6.347LeuAsp: 6.347 ± 0.088	6.1LeuGlu: 6.1 ± 0.087	3.653LeuPhe: 3.653 ± 0.076	8.265LeuGly: 8.265 ± 0.104	1.679LeuHis: 1.679 ± 0.043	4.385LeuIle: 4.385 ± 0.084	3.107LeuLys: 3.107 ± 0.059	9.129LeuLeu: 9.129 ± 0.142	2.144LeuMet: 2.144 ± 0.057	2.254LeuAsn: 2.254 ± 0.053	5.153LeuPro: 5.153 ± 0.085	2.491LeuGln: 2.491 ± 0.063	6.433LeuArg: 6.433 ± 0.091	5.808LeuSer: 5.808 ± 0.08	5.253LeuThr: 5.253 ± 0.078	7.547LeuVal: 7.547 ± 0.106	1.165LeuTrp: 1.165 ± 0.045	1.982LeuTyr: 1.982 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	3.188MetAla: 3.188 ± 0.068	0.148MetCys: 0.148 ± 0.014	1.294MetAsp: 1.294 ± 0.04	1.414MetGlu: 1.414 ± 0.038	0.657MetPhe: 0.657 ± 0.031	2.108MetGly: 2.108 ± 0.05	0.457MetHis: 0.457 ± 0.027	1.302MetIle: 1.302 ± 0.04	1.093MetLys: 1.093 ± 0.035	2.586MetLeu: 2.586 ± 0.05	0.686MetMet: 0.686 ± 0.028	0.766MetAsn: 0.766 ± 0.032	1.442MetPro: 1.442 ± 0.041	0.814MetGln: 0.814 ± 0.033	1.848MetArg: 1.848 ± 0.048	1.551MetSer: 1.551 ± 0.048	1.673MetThr: 1.673 ± 0.049	1.759MetVal: 1.759 ± 0.05	0.254MetTrp: 0.254 ± 0.018	0.304MetTyr: 0.304 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.213AsnAla: 3.213 ± 0.065	0.256AsnCys: 0.256 ± 0.018	1.648AsnAsp: 1.648 ± 0.081	1.536AsnGlu: 1.536 ± 0.044	1.036AsnPhe: 1.036 ± 0.039	2.474AsnGly: 2.474 ± 0.075	0.512AsnHis: 0.512 ± 0.025	1.369AsnIle: 1.369 ± 0.039	0.709AsnLys: 0.709 ± 0.033	2.568AsnLeu: 2.568 ± 0.055	0.604AsnMet: 0.604 ± 0.027	0.653AsnAsn: 0.653 ± 0.033	1.84AsnPro: 1.84 ± 0.052	0.798AsnGln: 0.798 ± 0.038	1.922AsnArg: 1.922 ± 0.048	1.406AsnSer: 1.406 ± 0.043	1.269AsnThr: 1.269 ± 0.044	1.769AsnVal: 1.769 ± 0.053	0.435AsnTrp: 0.435 ± 0.021	0.645AsnTyr: 0.645 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	5.443ProAla: 5.443 ± 0.097	0.311ProCys: 0.311 ± 0.019	3.646ProAsp: 3.646 ± 0.08	4.206ProGlu: 4.206 ± 0.075	1.882ProPhe: 1.882 ± 0.042	4.239ProGly: 4.239 ± 0.066	1.059ProHis: 1.059 ± 0.039	2.256ProIle: 2.256 ± 0.054	1.675ProLys: 1.675 ± 0.049	4.475ProLeu: 4.475 ± 0.077	1.175ProMet: 1.175 ± 0.038	1.169ProAsn: 1.169 ± 0.035	2.361ProPro: 2.361 ± 0.085	1.721ProGln: 1.721 ± 0.045	2.739ProArg: 2.739 ± 0.058	2.615ProSer: 2.615 ± 0.059	2.366ProThr: 2.366 ± 0.057	3.876ProVal: 3.876 ± 0.074	0.619ProTrp: 0.619 ± 0.026	1.058ProTyr: 1.058 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	3.884GlnAla: 3.884 ± 0.068	0.236GlnCys: 0.236 ± 0.017	1.663GlnAsp: 1.663 ± 0.046	1.688GlnGlu: 1.688 ± 0.044	1.23GlnPhe: 1.23 ± 0.039	2.527GlnGly: 2.527 ± 0.051	0.578GlnHis: 0.578 ± 0.024	1.63GlnIle: 1.63 ± 0.042	1.001GlnLys: 1.001 ± 0.039	3.167GlnLeu: 3.167 ± 0.063	0.879GlnMet: 0.879 ± 0.035	0.821GlnAsn: 0.821 ± 0.036	1.618GlnPro: 1.618 ± 0.043	1.227GlnGln: 1.227 ± 0.045	2.402GlnArg: 2.402 ± 0.051	1.759GlnSer: 1.759 ± 0.048	1.736GlnThr: 1.736 ± 0.047	2.38GlnVal: 2.38 ± 0.059	0.401GlnTrp: 0.401 ± 0.024	0.69GlnTyr: 0.69 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	7.451ArgAla: 7.451 ± 0.106	0.467ArgCys: 0.467 ± 0.023	4.158ArgAsp: 4.158 ± 0.071	5.159ArgGlu: 5.159 ± 0.085	2.844ArgPhe: 2.844 ± 0.057	4.942ArgGly: 4.942 ± 0.079	1.414ArgHis: 1.414 ± 0.035	4.01ArgIle: 4.01 ± 0.063	2.569ArgLys: 2.569 ± 0.06	7.333ArgLeu: 7.333 ± 0.1	1.969ArgMet: 1.969 ± 0.046	1.871ArgAsn: 1.871 ± 0.047	3.068ArgPro: 3.068 ± 0.063	2.384ArgGln: 2.384 ± 0.056	5.069ArgArg: 5.069 ± 0.096	3.711ArgSer: 3.711 ± 0.074	3.173ArgThr: 3.173 ± 0.062	4.529ArgVal: 4.529 ± 0.088	1.079ArgTrp: 1.079 ± 0.039	1.763ArgTyr: 1.763 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.026SerAla: 6.026 ± 0.09	0.448SerCys: 0.448 ± 0.038	3.635SerAsp: 3.635 ± 0.07	3.538SerGlu: 3.538 ± 0.066	2.147SerPhe: 2.147 ± 0.046	5.532SerGly: 5.532 ± 0.091	0.972SerHis: 0.972 ± 0.033	2.665SerIle: 2.665 ± 0.055	1.711SerLys: 1.711 ± 0.047	5.257SerLeu: 5.257 ± 0.084	1.321SerMet: 1.321 ± 0.039	1.483SerAsn: 1.483 ± 0.045	2.573SerPro: 2.573 ± 0.061	1.801SerGln: 1.801 ± 0.044	3.505SerArg: 3.505 ± 0.07	2.893SerSer: 2.893 ± 0.07	2.512SerThr: 2.512 ± 0.053	3.6SerVal: 3.6 ± 0.071	0.784SerTrp: 0.784 ± 0.03	1.343SerTyr: 1.343 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.699ThrAla: 5.699 ± 0.085	0.426ThrCys: 0.426 ± 0.022	2.996ThrAsp: 2.996 ± 0.069	2.826ThrGlu: 2.826 ± 0.047	1.861ThrPhe: 1.861 ± 0.046	5.273ThrGly: 5.273 ± 0.096	0.972ThrHis: 0.972 ± 0.03	2.976ThrIle: 2.976 ± 0.065	1.517ThrLys: 1.517 ± 0.044	5.552ThrLeu: 5.552 ± 0.099	1.256ThrMet: 1.256 ± 0.038	1.367ThrAsn: 1.367 ± 0.047	2.981ThrPro: 2.981 ± 0.058	1.55ThrGln: 1.55 ± 0.045	3.485ThrArg: 3.485 ± 0.062	2.803ThrSer: 2.803 ± 0.054	2.582ThrThr: 2.582 ± 0.069	3.89ThrVal: 3.89 ± 0.074	0.568ThrTrp: 0.568 ± 0.032	1.258ThrTyr: 1.258 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	8.379ValAla: 8.379 ± 0.103	0.578ValCys: 0.578 ± 0.024	4.405ValAsp: 4.405 ± 0.074	5.124ValGlu: 5.124 ± 0.088	2.316ValPhe: 2.316 ± 0.051	5.338ValGly: 5.338 ± 0.077	1.31ValHis: 1.31 ± 0.04	3.683ValIle: 3.683 ± 0.07	2.153ValLys: 2.153 ± 0.055	6.652ValLeu: 6.652 ± 0.101	1.75ValMet: 1.75 ± 0.048	1.868ValAsn: 1.868 ± 0.055	3.561ValPro: 3.561 ± 0.062	1.922ValGln: 1.922 ± 0.041	4.555ValArg: 4.555 ± 0.064	4.066ValSer: 4.066 ± 0.076	4.347ValThr: 4.347 ± 0.073	5.122ValVal: 5.122 ± 0.085	0.909ValTrp: 0.909 ± 0.026	1.335ValTyr: 1.335 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.282TrpAla: 1.282 ± 0.041	0.128TrpCys: 0.128 ± 0.012	0.829TrpAsp: 0.829 ± 0.034	0.726TrpGlu: 0.726 ± 0.032	0.594TrpPhe: 0.594 ± 0.024	1.064TrpGly: 1.064 ± 0.039	0.352TrpHis: 0.352 ± 0.019	0.701TrpIle: 0.701 ± 0.028	0.519TrpLys: 0.519 ± 0.025	1.747TrpLeu: 1.747 ± 0.053	0.372TrpMet: 0.372 ± 0.022	0.443TrpAsn: 0.443 ± 0.021	0.632TrpPro: 0.632 ± 0.026	0.601TrpGln: 0.601 ± 0.03	1.152TrpArg: 1.152 ± 0.034	0.881TrpSer: 0.881 ± 0.036	0.763TrpThr: 0.763 ± 0.028	0.824TrpVal: 0.824 ± 0.031	0.274TrpTrp: 0.274 ± 0.016	0.282TrpTyr: 0.282 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.511TyrAla: 2.511 ± 0.054	0.239TyrCys: 0.239 ± 0.016	1.599TyrAsp: 1.599 ± 0.043	1.471TyrGlu: 1.471 ± 0.04	0.881TyrPhe: 0.881 ± 0.036	2.21TyrGly: 2.21 ± 0.07	0.463TyrHis: 0.463 ± 0.023	0.94TyrIle: 0.94 ± 0.032	0.607TyrLys: 0.607 ± 0.029	2.152TyrLeu: 2.152 ± 0.052	0.415TyrMet: 0.415 ± 0.024	0.582TyrAsn: 0.582 ± 0.024	1.038TyrPro: 1.038 ± 0.033	0.645TyrGln: 0.645 ± 0.029	1.817TyrArg: 1.817 ± 0.051	1.301TyrSer: 1.301 ± 0.044	1.135TyrThr: 1.135 ± 0.04	1.457TyrVal: 1.457 ± 0.044	0.368TyrTrp: 0.368 ± 0.02	0.647TyrTyr: 0.647 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2938 proteins (896752 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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