Monoraphidium neglectum

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Sphaeropleales; Selenastraceae; Monoraphidium

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2MJY5|A0A0D2MJY5_9CHLO 20S proteasome subunit beta 6 OS=Monoraphidium neglectum OX=145388 GN=MNEG_4662 PE=4 SV=1
MM1 pKa = 7.72ALYY4 pKa = 10.68DD5 pKa = 4.05LFNNSGFRR13 pKa = 11.84HH14 pKa = 5.73YY15 pKa = 10.69ATGTPCFDD23 pKa = 3.04AHH25 pKa = 6.99DD26 pKa = 4.45ALRR29 pKa = 11.84DD30 pKa = 4.06PITPCEE36 pKa = 3.85ASVASGSSCFDD47 pKa = 4.27DD48 pKa = 4.45DD49 pKa = 4.73CAMSSTDD56 pKa = 3.98LFGASSSSRR65 pKa = 11.84SSADD69 pKa = 3.34SADD72 pKa = 3.92QPSLPLPRR80 pKa = 11.84SWFARR85 pKa = 11.84LSPQVPSCGIADD97 pKa = 4.05TPGGPSGGGDD107 pKa = 3.45FSSKK111 pKa = 10.51EE112 pKa = 3.47DD113 pKa = 3.18LAYY116 pKa = 10.7FNEE119 pKa = 4.08RR120 pKa = 11.84HH121 pKa = 6.07RR122 pKa = 11.84ARR124 pKa = 11.84LLSAGFSDD132 pKa = 6.53DD133 pKa = 3.85EE134 pKa = 4.46LCYY137 pKa = 10.94DD138 pKa = 5.44DD139 pKa = 6.22GGLLLGPIPFDD150 pKa = 4.05CSGDD154 pKa = 3.87DD155 pKa = 3.88GAGGAPAPRR164 pKa = 11.84PDD166 pKa = 3.84CPVVVDD172 pKa = 4.17EE173 pKa = 4.87QDD175 pKa = 3.99SISAAPGAYY184 pKa = 9.52DD185 pKa = 4.02AAFADD190 pKa = 4.81EE191 pKa = 4.29IDD193 pKa = 4.02AFFAAAGEE201 pKa = 4.51APFNPWRR208 pKa = 11.84QAPEE212 pKa = 4.04EE213 pKa = 4.25PFSFAAAAGPSGLGSVVGGGSLSFFMGVPLDD244 pKa = 4.21LEE246 pKa = 4.37IFSAPTAGGACAAVADD262 pKa = 4.22EE263 pKa = 4.3QVAISAAPGVSAGSGAKK280 pKa = 10.32GPAFDD285 pKa = 4.0FTFAGVGAEE294 pKa = 5.34GCCCWIGGDD303 pKa = 3.69VFAAASSGNGGGGSSGSSGDD323 pKa = 3.62SGSGSVGGNGGGGIGGGGGGGGDD346 pKa = 3.26PVAARR351 pKa = 11.84TRR353 pKa = 11.84SKK355 pKa = 11.19GGGAGASAAAA365 pKa = 4.19

Molecular weight: 35.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2MA77|A0A0D2MA77_9CHLO AP-1 complex subunit beta-1 OS=Monoraphidium neglectum OX=145388 GN=MNEG_7799 PE=4 SV=1
AA1 pKa = 7.34QRR3 pKa = 11.84AAPGRR8 pKa = 11.84LAARR12 pKa = 11.84PRR14 pKa = 11.84GGHH17 pKa = 6.82LGRR20 pKa = 11.84PVRR23 pKa = 11.84GRR25 pKa = 11.84PDD27 pKa = 2.61WRR29 pKa = 11.84AGRR32 pKa = 11.84PRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AGGRR41 pKa = 11.84HH42 pKa = 4.94GLAARR47 pKa = 11.84RR48 pKa = 11.84ARR50 pKa = 11.84RR51 pKa = 11.84GAGAGVARR59 pKa = 11.84ARR61 pKa = 11.84GGARR65 pKa = 11.84GGQQRR70 pKa = 11.84RR71 pKa = 11.84AGRR74 pKa = 11.84GRR76 pKa = 11.84LL77 pKa = 3.45

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16738

0

16738

5818762

47

7172

347.6

36.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.585 ± 0.051

1.491 ± 0.01

4.715 ± 0.014

5.353 ± 0.027

2.442 ± 0.013

10.513 ± 0.031

1.94 ± 0.01

2.347 ± 0.012

2.865 ± 0.018

9.499 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.785 ± 0.009

1.88 ± 0.014

6.405 ± 0.023

5.607 ± 0.038

6.928 ± 0.022

6.338 ± 0.018

4.059 ± 0.019

6.247 ± 0.018

1.341 ± 0.008

1.663 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski