Basidiobolus meristosporus CBS 931.73
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15513 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y1Y8E7|A0A1Y1Y8E7_9FUNG Alkaline phosphatase OS=Basidiobolus meristosporus CBS 931.73 OX=1314790 GN=K493DRAFT_315600 PE=3 SV=1
MM1 pKa = 8.09 DD2 pKa = 4.51 EE3 pKa = 5.25 NEE5 pKa = 4.75 GEE7 pKa = 4.21 DD8 pKa = 3.55 EE9 pKa = 5.05 GEE11 pKa = 4.94 GEE13 pKa = 5.38 DD14 pKa = 5.48 SMDD17 pKa = 3.85 DD18 pKa = 3.22 TSTQPEE24 pKa = 4.22 SSYY27 pKa = 11.86 NMPDD31 pKa = 3.35 FNIADD36 pKa = 3.81 MQPRR40 pKa = 11.84 KK41 pKa = 10.21 ALLDD45 pKa = 3.44 RR46 pKa = 11.84 VNAHH50 pKa = 6.43 SSSDD54 pKa = 3.34 ADD56 pKa = 3.52 ALSNSNQFDD65 pKa = 3.48 QAQVIDD71 pKa = 3.63 QTLYY75 pKa = 10.62 QPKK78 pKa = 9.11 QQQPQIIITQPATRR92 pKa = 11.84 SYY94 pKa = 11.7 GGGQKK99 pKa = 10.04 KK100 pKa = 9.31 YY101 pKa = 10.77 KK102 pKa = 10.06 RR103 pKa = 11.84 YY104 pKa = 9.73 SCSDD108 pKa = 3.37 QASDD112 pKa = 5.18 CYY114 pKa = 10.77 QQVQVQPTVEE124 pKa = 4.2 TPNAQVPLLRR134 pKa = 11.84 QMFTTVTRR142 pKa = 11.84 KK143 pKa = 9.69 HH144 pKa = 6.89 DD145 pKa = 3.96 NLNTANANSDD155 pKa = 3.05 KK156 pKa = 11.3 SLDD159 pKa = 3.62 VQFQAGPSLEE169 pKa = 4.37 VEE171 pKa = 4.99 GSASASEE178 pKa = 4.07 YY179 pKa = 10.89 AKK181 pKa = 10.7 QKK183 pKa = 10.29 GAEE186 pKa = 4.1 HH187 pKa = 6.93 SDD189 pKa = 3.7 DD190 pKa = 3.94 GSDD193 pKa = 3.53 YY194 pKa = 8.85 PTVMVQPAQDD204 pKa = 3.52 EE205 pKa = 5.18 TYY207 pKa = 8.91 STPVVDD213 pKa = 4.05 TQCIEE218 pKa = 4.09 GCSDD222 pKa = 3.12 QDD224 pKa = 3.6 TQYY227 pKa = 10.94 SEE229 pKa = 4.12 PMEE232 pKa = 5.12 DD233 pKa = 3.61 VQCVDD238 pKa = 3.78 DD239 pKa = 5.24 CANQDD244 pKa = 3.33 SQYY247 pKa = 9.58 TEE249 pKa = 4.1 PMEE252 pKa = 4.48 DD253 pKa = 3.32 TQQ255 pKa = 5.09
Molecular weight: 28.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.834
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.973
Patrickios 1.252
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A1Y1Y430|A0A1Y1Y430_9FUNG Uncharacterized protein OS=Basidiobolus meristosporus CBS 931.73 OX=1314790 GN=K493DRAFT_354074 PE=4 SV=1
KKK2 pKa = 10.14 HHH4 pKa = 4.91 CTLCSRR10 pKa = 11.84 SFQRR14 pKa = 11.84 PSALRR19 pKa = 11.84 IHHH22 pKa = 6.88 YYY24 pKa = 10.13 HHH26 pKa = 6.73 GEEE29 pKa = 4.38 KK30 pKa = 10.26 FEEE33 pKa = 4.05 SYYY36 pKa = 10.78 GCQKKK41 pKa = 10.49 FNVEEE46 pKa = 3.81 NLRR49 pKa = 11.84 RR50 pKa = 11.84 HHH52 pKa = 6.07 RR53 pKa = 11.84 TH
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.648
IPC_protein 10.555
Toseland 10.628
ProMoST 10.57
Dawson 10.745
Bjellqvist 10.511
Wikipedia 10.979
Rodwell 10.877
Grimsley 10.804
Solomon 10.891
Lehninger 10.847
Nozaki 10.687
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.052
Sillero 10.701
Patrickios 10.672
IPC_peptide 10.891
IPC2_peptide 9.97
IPC2.peptide.svr19 8.503
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15513
0
15513
5895669
49
8462
380.0
42.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.859 ± 0.017
1.506 ± 0.008
5.257 ± 0.016
6.487 ± 0.021
4.204 ± 0.015
6.202 ± 0.032
2.403 ± 0.009
5.886 ± 0.016
5.858 ± 0.021
9.409 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.009
4.558 ± 0.013
5.075 ± 0.019
4.013 ± 0.015
5.07 ± 0.015
8.181 ± 0.024
5.824 ± 0.017
6.378 ± 0.013
1.201 ± 0.007
3.407 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here