Ailuropoda melanoleuca papillomavirus 1
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGE4|A0A220IGE4_9PAPI Minor capsid protein L2 OS=Ailuropoda melanoleuca papillomavirus 1 OX=2016454 GN=L2 PE=3 SV=1
MM1 pKa = 8.05 EE2 pKa = 4.36 YY3 pKa = 10.16 PGTNGADD10 pKa = 4.37 FILQEE15 pKa = 4.67 ADD17 pKa = 3.78 CSDD20 pKa = 3.89 LSDD23 pKa = 3.63 EE24 pKa = 4.47 NFTEE28 pKa = 4.77 GEE30 pKa = 4.02 EE31 pKa = 4.13 DD32 pKa = 4.36 DD33 pKa = 5.11 EE34 pKa = 5.89 DD35 pKa = 5.92 FIDD38 pKa = 4.1 HH39 pKa = 6.86 ACVPTCSEE47 pKa = 4.58 EE48 pKa = 4.01 YY49 pKa = 10.88 LPMLTYY55 pKa = 9.32 QTEE58 pKa = 4.13 QADD61 pKa = 3.82 NEE63 pKa = 4.61 HH64 pKa = 6.11 IQLLKK69 pKa = 10.35 RR70 pKa = 11.84 KK71 pKa = 8.79 YY72 pKa = 9.98 VSPSPKK78 pKa = 9.33 MKK80 pKa = 10.73 LNVDD84 pKa = 3.95 LSPRR88 pKa = 11.84 LAAMQLTPEE97 pKa = 4.39 RR98 pKa = 11.84 KK99 pKa = 8.6 AKK101 pKa = 10.17 RR102 pKa = 11.84 RR103 pKa = 11.84 LFEE106 pKa = 5.13 HH107 pKa = 6.92 SDD109 pKa = 3.11 SGYY112 pKa = 11.17 GNTLEE117 pKa = 4.39 AQSEE121 pKa = 4.31 EE122 pKa = 4.27 KK123 pKa = 9.99 MGEE126 pKa = 3.97 EE127 pKa = 4.27 TQVEE131 pKa = 4.34 TGEE134 pKa = 4.22 EE135 pKa = 4.17 EE136 pKa = 4.08 EE137 pKa = 4.69 EE138 pKa = 4.12 EE139 pKa = 4.25 AGEE142 pKa = 5.04 DD143 pKa = 4.08 GGSQCIEE150 pKa = 4.11 NGVGRR155 pKa = 11.84 ATEE158 pKa = 3.9 NALPIQLIKK167 pKa = 11.17 SKK169 pKa = 10.61 DD170 pKa = 3.38 SRR172 pKa = 11.84 VAKK175 pKa = 10.22 LGKK178 pKa = 10.17 FKK180 pKa = 9.98 EE181 pKa = 4.67 TYY183 pKa = 9.92 DD184 pKa = 3.44 VSFGDD189 pKa = 3.6 LCRR192 pKa = 11.84 PFKK195 pKa = 10.66 SDD197 pKa = 2.9 KK198 pKa = 9.82 TVNAEE203 pKa = 3.95 WVCAAFGVHH212 pKa = 7.4 DD213 pKa = 4.76 SVCDD217 pKa = 4.1 SIQEE221 pKa = 4.14 VLKK224 pKa = 10.62 SYY226 pKa = 9.61 CTFTHH231 pKa = 6.08 ITCEE235 pKa = 3.79 QSSWGFILLMLLCFTTGKK253 pKa = 10.29 CRR255 pKa = 11.84 TTVEE259 pKa = 4.44 KK260 pKa = 10.81 LLSTLLAIKK269 pKa = 9.7 TEE271 pKa = 4.38 ALLSDD276 pKa = 3.91 PPKK279 pKa = 10.33 VRR281 pKa = 11.84 SGAAAMYY288 pKa = 8.6 WYY290 pKa = 10.61 KK291 pKa = 11.42 GMLTNTSAVWGEE303 pKa = 3.86 KK304 pKa = 9.92 PKK306 pKa = 10.38 WITTQVSLAHH316 pKa = 5.99 ITSEE320 pKa = 4.01 NVPFDD325 pKa = 4.31 LSAMVQWAYY334 pKa = 11.05 DD335 pKa = 3.44 NEE337 pKa = 4.36 YY338 pKa = 10.44 TEE340 pKa = 4.71 EE341 pKa = 4.02 CQIAYY346 pKa = 9.52 EE347 pKa = 4.21 YY348 pKa = 11.45 ASLADD353 pKa = 3.8 VEE355 pKa = 4.53 PNAAAFLKK363 pKa = 10.91 SNSQAKK369 pKa = 8.88 HH370 pKa = 5.7 VKK372 pKa = 9.92 DD373 pKa = 3.5 CAIMCRR379 pKa = 11.84 HH380 pKa = 5.81 YY381 pKa = 10.91 KK382 pKa = 8.96 RR383 pKa = 11.84 AEE385 pKa = 3.88 MQRR388 pKa = 11.84 MSMRR392 pKa = 11.84 QWIEE396 pKa = 3.6 RR397 pKa = 11.84 QRR399 pKa = 11.84 DD400 pKa = 3.82 RR401 pKa = 11.84 ISGEE405 pKa = 4.14 GDD407 pKa = 2.66 WKK409 pKa = 9.78 TIVYY413 pKa = 8.79 FLKK416 pKa = 10.04 YY417 pKa = 9.73 QDD419 pKa = 3.82 VEE421 pKa = 4.42 FVPFMVKK428 pKa = 10.49 FKK430 pKa = 10.96 LFLKK434 pKa = 9.93 GIPKK438 pKa = 10.02 KK439 pKa = 10.51 NCLVFYY445 pKa = 9.99 GPPNTGKK452 pKa = 10.3 SAFCMSLLKK461 pKa = 10.8 FFGGKK466 pKa = 8.76 VVSFMNSRR474 pKa = 11.84 SHH476 pKa = 7.31 FWLQPLLDD484 pKa = 4.56 GKK486 pKa = 11.01 LGLLDD491 pKa = 5.31 DD492 pKa = 4.61 ATDD495 pKa = 3.72 VCWDD499 pKa = 3.66 FFDD502 pKa = 5.26 AYY504 pKa = 10.65 LRR506 pKa = 11.84 NAIDD510 pKa = 4.06 GNPVSVDD517 pKa = 3.32 SKK519 pKa = 11.03 HH520 pKa = 6.79 RR521 pKa = 11.84 APQQVKK527 pKa = 10.24 LPPLLVTTNIDD538 pKa = 2.81 IRR540 pKa = 11.84 KK541 pKa = 7.42 HH542 pKa = 5.62 VKK544 pKa = 8.02 WQYY547 pKa = 9.15 LHH549 pKa = 7.15 SRR551 pKa = 11.84 VQLVNFANEE560 pKa = 3.96 FPITDD565 pKa = 4.27 DD566 pKa = 3.92 GNCVFDD572 pKa = 3.45 FHH574 pKa = 6.36 EE575 pKa = 4.83 RR576 pKa = 11.84 NWKK579 pKa = 10.28 AFFGKK584 pKa = 9.64 VWARR588 pKa = 11.84 LDD590 pKa = 3.72 LSDD593 pKa = 6.34 DD594 pKa = 3.53 EE595 pKa = 5.94 DD596 pKa = 3.47 EE597 pKa = 5.69 GYY599 pKa = 10.87 DD600 pKa = 3.58 GGSEE604 pKa = 3.77 QTFRR608 pKa = 11.84 CTARR612 pKa = 11.84 KK613 pKa = 9.27 NDD615 pKa = 3.52 GVII618 pKa = 3.41
Molecular weight: 70.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.976
IPC2_protein 5.029
IPC_protein 4.991
Toseland 4.914
ProMoST 5.13
Dawson 4.978
Bjellqvist 5.105
Wikipedia 4.838
Rodwell 4.889
Grimsley 4.838
Solomon 4.978
Lehninger 4.927
Nozaki 5.092
DTASelect 5.245
Thurlkill 4.914
EMBOSS 4.876
Sillero 5.169
Patrickios 3.91
IPC_peptide 4.991
IPC2_peptide 5.156
IPC2.peptide.svr19 5.154
Protein with the highest isoelectric point:
>tr|A0A220IGF5|A0A220IGF5_9PAPI Major capsid protein L1 OS=Ailuropoda melanoleuca papillomavirus 1 OX=2016454 GN=L1 PE=3 SV=1
MM1 pKa = 7.84 EE2 pKa = 6.04 DD3 pKa = 2.95 LSKK6 pKa = 11.09 RR7 pKa = 11.84 LGALQEE13 pKa = 4.36 KK14 pKa = 9.89 MMEE17 pKa = 4.15 LYY19 pKa = 7.97 EE20 pKa = 4.37 TGSASLRR27 pKa = 11.84 TQVIYY32 pKa = 10.18 WLLMRR37 pKa = 11.84 KK38 pKa = 7.92 EE39 pKa = 3.94 QALLYY44 pKa = 10.18 HH45 pKa = 7.03 ARR47 pKa = 11.84 QCGANRR53 pKa = 11.84 LGMCAVPPLQVSKK66 pKa = 11.14 ARR68 pKa = 11.84 ASKK71 pKa = 9.94 AIKK74 pKa = 8.48 MHH76 pKa = 6.59 LVLTSLEE83 pKa = 3.98 GSKK86 pKa = 10.96 YY87 pKa = 10.8 GLLPWTMRR95 pKa = 11.84 EE96 pKa = 3.89 TSYY99 pKa = 11.29 EE100 pKa = 3.99 RR101 pKa = 11.84 YY102 pKa = 9.28 KK103 pKa = 11.29 ANPKK107 pKa = 8.48 NTLKK111 pKa = 10.95 KK112 pKa = 10.05 EE113 pKa = 4.13 GVPVQVLYY121 pKa = 10.98 DD122 pKa = 3.55 GEE124 pKa = 4.25 KK125 pKa = 10.36 AKK127 pKa = 10.79 GVEE130 pKa = 4.23 YY131 pKa = 10.68 VLWTSVFAQGSCGKK145 pKa = 7.06 WTCFQGGVDD154 pKa = 3.81 SSGLYY159 pKa = 9.42 VDD161 pKa = 3.83 KK162 pKa = 10.8 EE163 pKa = 4.17 GRR165 pKa = 11.84 KK166 pKa = 9.26 QYY168 pKa = 11.3 YY169 pKa = 10.2 EE170 pKa = 5.0 DD171 pKa = 3.81 FQKK174 pKa = 11.27 DD175 pKa = 3.48 SLKK178 pKa = 11.12 YY179 pKa = 9.11 GVKK182 pKa = 10.39 GVWTVVFGNEE192 pKa = 3.94 TLYY195 pKa = 11.24 SSASPQPCEE204 pKa = 3.82 QPFPAVVDD212 pKa = 3.96 TTDD215 pKa = 3.66 EE216 pKa = 4.28 VPPFCSPSPEE226 pKa = 4.16 KK227 pKa = 10.94 AEE229 pKa = 4.04 EE230 pKa = 3.76 QALPSKK236 pKa = 10.55 PKK238 pKa = 10.19 GSSGSSPRR246 pKa = 11.84 FGKK249 pKa = 9.55 QGKK252 pKa = 9.42 RR253 pKa = 11.84 GSSKK257 pKa = 10.52 RR258 pKa = 11.84 KK259 pKa = 9.27 LSEE262 pKa = 3.83 EE263 pKa = 4.59 GEE265 pKa = 4.3 QQGQQQQQQLKK276 pKa = 10.07 KK277 pKa = 9.7 PYY279 pKa = 9.15 CEE281 pKa = 4.01 QPASEE286 pKa = 4.87 HH287 pKa = 6.2 CNNWTKK293 pKa = 10.66 GRR295 pKa = 11.84 QQEE298 pKa = 4.16 QQEE301 pKa = 4.27 QQQQQQQQQQQRR313 pKa = 11.84 QHH315 pKa = 5.65 NANSDD320 pKa = 3.57 RR321 pKa = 11.84 HH322 pKa = 4.9 VQWSGVNSPINVDD335 pKa = 3.78 DD336 pKa = 4.23 EE337 pKa = 4.5 QEE339 pKa = 4.06 EE340 pKa = 4.65 GQEE343 pKa = 4.1 EE344 pKa = 4.25 QQQQQQQQQQQVQQQPSTRR363 pKa = 11.84 NCDD366 pKa = 2.92 ASGPRR371 pKa = 11.84 VSQEE375 pKa = 3.83 SSSPRR380 pKa = 11.84 PPAPLPVVVLSGNSNQVKK398 pKa = 9.37 CLRR401 pKa = 11.84 YY402 pKa = 9.37 RR403 pKa = 11.84 LYY405 pKa = 11.06 KK406 pKa = 10.05 KK407 pKa = 10.46 FKK409 pKa = 10.47 HH410 pKa = 5.86 SVLDD414 pKa = 5.35 ISSTWCWTSPGCPTAAKK431 pKa = 8.44 ITVIFASSSQRR442 pKa = 11.84 EE443 pKa = 4.16 DD444 pKa = 3.39 FLSKK448 pKa = 9.44 VTLPKK453 pKa = 10.72 GVTFALGEE461 pKa = 4.03 LPYY464 pKa = 11.18
Molecular weight: 52.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.636
IPC2_protein 7.936
IPC_protein 7.746
Toseland 7.41
ProMoST 8.404
Dawson 8.521
Bjellqvist 8.814
Wikipedia 8.463
Rodwell 8.58
Grimsley 7.38
Solomon 8.595
Lehninger 8.609
Nozaki 9.019
DTASelect 8.565
Thurlkill 8.639
EMBOSS 8.726
Sillero 8.946
Patrickios 4.368
IPC_peptide 8.595
IPC2_peptide 7.644
IPC2.peptide.svr19 7.938
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2209
140
618
441.8
49.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.794 ± 0.322
2.49 ± 0.737
5.613 ± 0.766
5.976 ± 0.877
4.346 ± 0.479
6.655 ± 0.474
1.901 ± 0.237
3.893 ± 0.769
5.93 ± 0.843
7.967 ± 0.693
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.856 ± 0.398
3.531 ± 0.629
6.383 ± 1.112
5.976 ± 1.536
5.115 ± 0.415
8.873 ± 1.239
6.066 ± 0.701
6.474 ± 0.201
1.584 ± 0.294
3.576 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here