Ageratum yellow vein virus-Ishigaki

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Ageratum yellow vein virus

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8CFD9|A8CFD9_9GEMI C4 protein OS=Ageratum yellow vein virus-Ishigaki OX=446318 GN=C4 PE=3 SV=1
MM1 pKa = 7.7APPRR5 pKa = 11.84PFKK8 pKa = 10.85INAKK12 pKa = 10.09NYY14 pKa = 8.77FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.55EE26 pKa = 4.13EE27 pKa = 4.57TLSQIQALDD36 pKa = 3.36TPTNKK41 pKa = 10.1KK42 pKa = 9.7YY43 pKa = 10.84IKK45 pKa = 9.66ICRR48 pKa = 11.84EE49 pKa = 3.74LHH51 pKa = 6.25EE52 pKa = 5.87DD53 pKa = 4.22GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.99GKK67 pKa = 8.83YY68 pKa = 8.62QCKK71 pKa = 10.05NNRR74 pKa = 11.84FFDD77 pKa = 4.99LISPSRR83 pKa = 11.84SAHH86 pKa = 4.9FHH88 pKa = 6.45PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.4AYY134 pKa = 10.68AQALNSGSKK143 pKa = 10.2SEE145 pKa = 4.05ALNVIKK151 pKa = 10.37EE152 pKa = 4.29LAPKK156 pKa = 10.3DD157 pKa = 3.74YY158 pKa = 11.2VLQFHH163 pKa = 6.76NLNANLDD170 pKa = 4.25RR171 pKa = 11.84IFAPPLEE178 pKa = 4.42VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.95EE195 pKa = 4.04LEE197 pKa = 3.96EE198 pKa = 4.17WVSEE202 pKa = 4.26NVSGAAARR210 pKa = 11.84PWRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.03KK312 pKa = 10.55EE313 pKa = 3.94YY314 pKa = 10.94LDD316 pKa = 3.87EE317 pKa = 4.82EE318 pKa = 4.68KK319 pKa = 11.13NSALKK324 pKa = 10.19EE325 pKa = 3.96WAIKK329 pKa = 9.74NAEE332 pKa = 4.48FITLNRR338 pKa = 11.84PLYY341 pKa = 10.87SGTNQSAAQNSEE353 pKa = 4.29EE354 pKa = 4.32EE355 pKa = 4.42ASSQAA360 pKa = 3.47

Molecular weight: 40.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8CFD6|A8CFD6_9GEMI Replication enhancer OS=Ageratum yellow vein virus-Ishigaki OX=446318 GN=C3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.46TPVMSRR30 pKa = 11.84AAAPTVLVTNRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84TWTNRR48 pKa = 11.84PMYY51 pKa = 9.79RR52 pKa = 11.84KK53 pKa = 8.55PRR55 pKa = 11.84LYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 9.71RR61 pKa = 11.84TPDD64 pKa = 3.19VPKK67 pKa = 10.64GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 6.94EE79 pKa = 3.74QRR81 pKa = 11.84HH82 pKa = 6.38DD83 pKa = 3.16ISHH86 pKa = 5.75VGKK89 pKa = 9.75VLCVSDD95 pKa = 3.49VTRR98 pKa = 11.84GNGLTHH104 pKa = 6.89RR105 pKa = 11.84VGKK108 pKa = 9.66RR109 pKa = 11.84FCVKK113 pKa = 9.94SVYY116 pKa = 10.52VLGKK120 pKa = 9.41IWMDD124 pKa = 3.35EE125 pKa = 4.04NIKK128 pKa = 9.32TKK130 pKa = 10.61NHH132 pKa = 5.92TNTVMFYY139 pKa = 10.33LVRR142 pKa = 11.84DD143 pKa = 3.68RR144 pKa = 11.84RR145 pKa = 11.84PFGTAMDD152 pKa = 4.48FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.36PSTATIKK170 pKa = 10.89NDD172 pKa = 3.0LRR174 pKa = 11.84DD175 pKa = 3.56RR176 pKa = 11.84YY177 pKa = 9.85QVLRR181 pKa = 11.84KK182 pKa = 8.25FTSTVTGGQYY192 pKa = 10.82ASKK195 pKa = 10.24EE196 pKa = 3.73QALVKK201 pKa = 10.62KK202 pKa = 10.37FMKK205 pKa = 9.91INNYY209 pKa = 8.31VVYY212 pKa = 10.35NHH214 pKa = 6.06QEE216 pKa = 3.36AAKK219 pKa = 10.14YY220 pKa = 10.5DD221 pKa = 3.75NHH223 pKa = 6.5TEE225 pKa = 4.02NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.33DD253 pKa = 3.43SVQNN257 pKa = 3.87

Molecular weight: 29.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1098

96

360

183.0

21.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.464 ± 0.826

2.459 ± 0.574

5.191 ± 0.406

4.736 ± 0.939

4.007 ± 0.453

4.372 ± 0.492

3.825 ± 0.759

5.647 ± 0.885

5.282 ± 1.116

7.377 ± 0.642

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.649

6.193 ± 0.616

5.92 ± 0.783

5.191 ± 0.88

7.013 ± 1.301

7.559 ± 1.358

6.466 ± 1.267

5.738 ± 1.267

1.366 ± 0.332

4.007 ± 0.755
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski