Salipaludibacillus neizhouensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Salipaludibacillus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4767 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9KBN9|A0A3A9KBN9_9BACI LMWPc domain-containing protein OS=Salipaludibacillus neizhouensis OX=885475 GN=CR203_24580 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.29KK3 pKa = 9.41ILLIIGSSLLVALVSACGSNNEE25 pKa = 4.16VSNAGNNTNQSSSDD39 pKa = 3.31QTLTVLVEE47 pKa = 4.52GGSPAQTVADD57 pKa = 3.77LTKK60 pKa = 11.06EE61 pKa = 3.94EE62 pKa = 4.49FEE64 pKa = 4.59EE65 pKa = 4.11NTGYY69 pKa = 10.72EE70 pKa = 4.36VIIEE74 pKa = 4.26SVPYY78 pKa = 10.06SGVYY82 pKa = 10.55DD83 pKa = 3.98RR84 pKa = 11.84MRR86 pKa = 11.84TEE88 pKa = 4.07LTSGAGAFDD97 pKa = 3.72VATIDD102 pKa = 4.5TIWLPALYY110 pKa = 9.93QGLEE114 pKa = 4.31PIQEE118 pKa = 4.45EE119 pKa = 4.51ITDD122 pKa = 3.83EE123 pKa = 4.17MQDD126 pKa = 3.61DD127 pKa = 5.18LFPGLIDD134 pKa = 3.97GASMDD139 pKa = 3.76GNVYY143 pKa = 10.61GLPTWTNSKK152 pKa = 9.89ILLYY156 pKa = 10.65RR157 pKa = 11.84EE158 pKa = 4.69DD159 pKa = 5.07LFNDD163 pKa = 3.76SNNQDD168 pKa = 3.05EE169 pKa = 4.71FLDD172 pKa = 3.69EE173 pKa = 4.71FGYY176 pKa = 10.64EE177 pKa = 4.09LSPPEE182 pKa = 3.87TWDD185 pKa = 3.09QYY187 pKa = 11.82KK188 pKa = 10.69DD189 pKa = 3.38VAHH192 pKa = 6.69FFGNNEE198 pKa = 4.44NYY200 pKa = 9.77PEE202 pKa = 4.52LYY204 pKa = 8.15GTSVFGATSGDD215 pKa = 4.25SVASWLDD222 pKa = 3.3HH223 pKa = 5.53VAQAGAEE230 pKa = 3.81ALVINDD236 pKa = 3.56AGEE239 pKa = 4.32VIVNDD244 pKa = 4.41DD245 pKa = 3.62DD246 pKa = 4.66HH247 pKa = 7.67VAALEE252 pKa = 3.9FLNDD256 pKa = 3.67LVNEE260 pKa = 4.22EE261 pKa = 4.4GVVPEE266 pKa = 4.77GVLEE270 pKa = 4.13TASAEE275 pKa = 4.21TSEE278 pKa = 5.0LFYY281 pKa = 11.31NGNLAMMLAWGHH293 pKa = 5.91FFVPSNDD300 pKa = 3.53PDD302 pKa = 3.8TSDD305 pKa = 3.17VAGDD309 pKa = 3.37VGAAPMIAGDD319 pKa = 3.8GGIGVVPGPWYY330 pKa = 10.32QVIPSSSEE338 pKa = 3.63KK339 pKa = 10.38KK340 pKa = 10.63DD341 pKa = 3.12IAKK344 pKa = 10.24EE345 pKa = 3.84YY346 pKa = 11.26LLFLYY351 pKa = 9.96EE352 pKa = 4.13NNEE355 pKa = 4.13LYY357 pKa = 11.04ADD359 pKa = 4.08ALGVAARR366 pKa = 11.84QSVFEE371 pKa = 4.53EE372 pKa = 4.23YY373 pKa = 10.71GQQEE377 pKa = 4.84EE378 pKa = 4.69YY379 pKa = 11.56AHH381 pKa = 6.6LNEE384 pKa = 5.65LATTLNGKK392 pKa = 7.08QTQNRR397 pKa = 11.84PSTEE401 pKa = 3.22KK402 pKa = 9.79WEE404 pKa = 4.56EE405 pKa = 4.0IEE407 pKa = 5.65SEE409 pKa = 4.19ALLPAVQNVFSGSQTPQEE427 pKa = 4.51ALDD430 pKa = 3.75EE431 pKa = 4.43AKK433 pKa = 10.51EE434 pKa = 4.01IIEE437 pKa = 4.83GIVNN441 pKa = 3.98

Molecular weight: 47.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9JYW1|A0A3A9JYW1_9BACI Iron-siderophore ABC transporter permease OS=Salipaludibacillus neizhouensis OX=885475 GN=CR203_20735 PE=3 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.34KK15 pKa = 9.1NHH17 pKa = 4.73GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.24NGRR29 pKa = 11.84HH30 pKa = 5.46ILANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.72GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4767

0

4767

1414254

23

2034

296.7

33.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.402 ± 0.036

0.668 ± 0.011

5.376 ± 0.03

7.856 ± 0.044

4.664 ± 0.028

6.805 ± 0.034

2.121 ± 0.017

7.914 ± 0.039

6.62 ± 0.036

9.656 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.019

4.672 ± 0.027

3.498 ± 0.02

3.562 ± 0.021

3.919 ± 0.024

6.442 ± 0.026

5.483 ± 0.021

6.914 ± 0.027

1.099 ± 0.013

3.505 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski