Metarhizium acridum (strain CQMa 102)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium; Metarhizium acridum

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9830 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9EBH2|E9EBH2_METAQ Acyl CoA binding family protein OS=Metarhizium acridum (strain CQMa 102) OX=655827 GN=MAC_07220 PE=4 SV=1
MM1 pKa = 7.63PHH3 pKa = 7.37RR4 pKa = 11.84IDD6 pKa = 4.22TPPLPPPPPSSSSPQAPRR24 pKa = 11.84SPIEE28 pKa = 4.54DD29 pKa = 3.99DD30 pKa = 3.51YY31 pKa = 11.98FDD33 pKa = 3.13IHH35 pKa = 7.35RR36 pKa = 11.84YY37 pKa = 9.3YY38 pKa = 11.19YY39 pKa = 10.41EE40 pKa = 4.06LDD42 pKa = 3.26RR43 pKa = 11.84YY44 pKa = 10.44YY45 pKa = 11.54VFADD49 pKa = 3.71DD50 pKa = 4.42SDD52 pKa = 4.46YY53 pKa = 11.53NYY55 pKa = 10.92SEE57 pKa = 5.05SDD59 pKa = 3.17DD60 pKa = 4.06SEE62 pKa = 4.8YY63 pKa = 11.15DD64 pKa = 3.65GPEE67 pKa = 3.85QPDD70 pKa = 3.7DD71 pKa = 4.02EE72 pKa = 5.01GADD75 pKa = 3.57DD76 pKa = 3.85EE77 pKa = 4.92TYY79 pKa = 10.25EE80 pKa = 4.14VPRR83 pKa = 11.84NFSIRR88 pKa = 11.84LWVEE92 pKa = 3.75DD93 pKa = 3.99LSQFTNVRR101 pKa = 11.84AQVDD105 pKa = 3.85SLFFPTSIQVQKK117 pKa = 10.6YY118 pKa = 9.89LNYY121 pKa = 10.42DD122 pKa = 3.43SLFSVVLTWDD132 pKa = 3.34DD133 pKa = 3.49VEE135 pKa = 5.36YY136 pKa = 10.79DD137 pKa = 4.1GQPLDD142 pKa = 3.59PFVAEE147 pKa = 4.07EE148 pKa = 4.23QILEE152 pKa = 4.18HH153 pKa = 6.24HH154 pKa = 6.73SGRR157 pKa = 11.84IRR159 pKa = 11.84FTEE162 pKa = 3.85

Molecular weight: 19.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9EBQ5|E9EBQ5_METAQ Uncharacterized protein OS=Metarhizium acridum (strain CQMa 102) OX=655827 GN=MAC_07303 PE=4 SV=1
MM1 pKa = 7.18NAPWGPIIPNLAMGLVHH18 pKa = 6.84FGIPGSFQHH27 pKa = 7.06EE28 pKa = 4.62INTLTIQHH36 pKa = 6.13QDD38 pKa = 3.12AKK40 pKa = 10.65NSTSGGIGSGYY51 pKa = 8.01TVPEE55 pKa = 4.3DD56 pKa = 3.41AVARR60 pKa = 11.84AASGLHH66 pKa = 5.81RR67 pKa = 11.84LPFRR71 pKa = 11.84WARR74 pKa = 11.84HH75 pKa = 3.78WLINATTSPTHH86 pKa = 6.22GSRR89 pKa = 11.84FAGSPEE95 pKa = 4.21LRR97 pKa = 11.84PGPQQPRR104 pKa = 11.84NHH106 pKa = 6.89WPPVVASQSNSQSTGILRR124 pKa = 11.84RR125 pKa = 11.84LGGTRR130 pKa = 11.84IFLCRR135 pKa = 11.84LDD137 pKa = 3.81STCGVGQVHH146 pKa = 6.89GKK148 pKa = 8.78PLNPSATGATFSFPFCFLVKK168 pKa = 10.04SWGFNRR174 pKa = 11.84NLALVPQVLCLFQVKK189 pKa = 8.77PQASPSVHH197 pKa = 5.6RR198 pKa = 11.84VRR200 pKa = 11.84LRR202 pKa = 11.84FLACIYY208 pKa = 10.38GKK210 pKa = 10.41RR211 pKa = 11.84PTPLSEE217 pKa = 3.6PAYY220 pKa = 9.88ILLRR224 pKa = 11.84LSNPEE229 pKa = 4.26APVLDD234 pKa = 3.74QTLVPVFDD242 pKa = 4.59PCPLTVFLVHH252 pKa = 6.91SRR254 pKa = 11.84AQSHH258 pKa = 6.28GSNPPKK264 pKa = 10.33VSNRR268 pKa = 11.84ALSEE272 pKa = 3.81NHH274 pKa = 6.65LGLNTPAASLQLGSVIFF291 pKa = 4.09

Molecular weight: 31.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9830

0

9830

4847146

44

10285

493.1

54.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.873 ± 0.022

1.268 ± 0.009

5.841 ± 0.017

5.998 ± 0.024

3.632 ± 0.015

7.016 ± 0.023

2.434 ± 0.012

4.716 ± 0.02

4.907 ± 0.026

8.829 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.009

3.66 ± 0.021

6.008 ± 0.03

4.046 ± 0.019

6.239 ± 0.022

8.133 ± 0.026

5.804 ± 0.018

6.242 ± 0.021

1.459 ± 0.009

2.66 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski