Novosphingobium sp. TH158

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2854 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N4X5H4|A0A2N4X5H4_9SPHN Uncharacterized protein OS=Novosphingobium sp. TH158 OX=2067455 GN=C0V78_04375 PE=4 SV=1
MM1 pKa = 7.59VDD3 pKa = 3.78LSQYY7 pKa = 11.02LPILIFLVIALGLSAAFVFLPMGVSFLTGTHH38 pKa = 6.04NPNAEE43 pKa = 4.04KK44 pKa = 10.67NSEE47 pKa = 4.24YY48 pKa = 10.89EE49 pKa = 4.01CGFPAFEE56 pKa = 4.86DD57 pKa = 3.7PRR59 pKa = 11.84SQFDD63 pKa = 3.17VRR65 pKa = 11.84FYY67 pKa = 11.3LVAILFIVFDD77 pKa = 4.56LEE79 pKa = 4.43AAFLFPWAISLDD91 pKa = 3.8FTGWAGWITMMIFLGEE107 pKa = 4.0LAIGLAYY114 pKa = 9.76AWKK117 pKa = 10.44VGALDD122 pKa = 3.63WEE124 pKa = 4.55

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N4WV92|A0A2N4WV92_9SPHN Dihydroorotase OS=Novosphingobium sp. TH158 OX=2067455 GN=C0V78_13180 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2854

0

2854

910234

36

4165

318.9

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.29 ± 0.074

0.903 ± 0.013

5.569 ± 0.032

5.727 ± 0.053

3.505 ± 0.028

9.167 ± 0.068

1.999 ± 0.024

4.768 ± 0.037

3.273 ± 0.042

9.942 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.021

2.609 ± 0.04

5.39 ± 0.036

3.129 ± 0.022

7.076 ± 0.057

5.275 ± 0.043

4.976 ± 0.057

7.209 ± 0.038

1.501 ± 0.018

2.141 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski