Amino acid dipepetide frequency for Novosphingobium sp. TH158

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.828AlaAla: 18.828 ± 0.208
1.239AlaCys: 1.239 ± 0.039
7.217AlaAsp: 7.217 ± 0.102
8.332AlaGlu: 8.332 ± 0.125
4.213AlaPhe: 4.213 ± 0.075
12.121AlaGly: 12.121 ± 0.151
2.233AlaHis: 2.233 ± 0.058
6.533AlaIle: 6.533 ± 0.093
4.32AlaLys: 4.32 ± 0.095
14.112AlaLeu: 14.112 ± 0.192
4.24AlaMet: 4.24 ± 0.074
3.271AlaAsn: 3.271 ± 0.068
6.234AlaPro: 6.234 ± 0.121
4.646AlaGln: 4.646 ± 0.096
9.98AlaArg: 9.98 ± 0.131
6.731AlaSer: 6.731 ± 0.097
5.874AlaThr: 5.874 ± 0.102
8.591AlaVal: 8.591 ± 0.101
1.734AlaTrp: 1.734 ± 0.047
2.45AlaTyr: 2.45 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
1.14CysAla: 1.14 ± 0.037
0.105CysCys: 0.105 ± 0.011
0.627CysAsp: 0.627 ± 0.025
0.509CysGlu: 0.509 ± 0.024
0.318CysPhe: 0.318 ± 0.02
0.989CysGly: 0.989 ± 0.038
0.246CysHis: 0.246 ± 0.018
0.36CysIle: 0.36 ± 0.021
0.249CysLys: 0.249 ± 0.016
0.756CysLeu: 0.756 ± 0.031
0.185CysMet: 0.185 ± 0.012
0.265CysAsn: 0.265 ± 0.017
0.526CysPro: 0.526 ± 0.027
0.234CysGln: 0.234 ± 0.016
0.626CysArg: 0.626 ± 0.026
0.502CysSer: 0.502 ± 0.024
0.481CysThr: 0.481 ± 0.025
0.586CysVal: 0.586 ± 0.026
0.12CysTrp: 0.12 ± 0.013
0.208CysTyr: 0.208 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
6.984AspAla: 6.984 ± 0.089
0.583AspCys: 0.583 ± 0.024
2.763AspAsp: 2.763 ± 0.063
3.415AspGlu: 3.415 ± 0.067
2.131AspPhe: 2.131 ± 0.047
5.274AspGly: 5.274 ± 0.09
1.235AspHis: 1.235 ± 0.038
2.54AspIle: 2.54 ± 0.057
1.916AspLys: 1.916 ± 0.051
5.483AspLeu: 5.483 ± 0.082
1.458AspMet: 1.458 ± 0.038
1.334AspAsn: 1.334 ± 0.04
3.807AspPro: 3.807 ± 0.08
1.581AspGln: 1.581 ± 0.041
4.274AspArg: 4.274 ± 0.071
2.281AspSer: 2.281 ± 0.053
2.281AspThr: 2.281 ± 0.051
3.672AspVal: 3.672 ± 0.076
1.133AspTrp: 1.133 ± 0.036
1.542AspTyr: 1.542 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
8.453GluAla: 8.453 ± 0.108
0.428GluCys: 0.428 ± 0.02
2.815GluAsp: 2.815 ± 0.058
3.374GluGlu: 3.374 ± 0.071
1.752GluPhe: 1.752 ± 0.047
4.912GluGly: 4.912 ± 0.087
1.19GluHis: 1.19 ± 0.039
2.816GluIle: 2.816 ± 0.058
2.293GluLys: 2.293 ± 0.057
5.511GluLeu: 5.511 ± 0.089
1.499GluMet: 1.499 ± 0.044
1.382GluAsn: 1.382 ± 0.036
2.748GluPro: 2.748 ± 0.062
2.07GluGln: 2.07 ± 0.052
4.742GluArg: 4.742 ± 0.093
2.396GluSer: 2.396 ± 0.057
2.868GluThr: 2.868 ± 0.051
4.03GluVal: 4.03 ± 0.07
0.946GluTrp: 0.946 ± 0.03
1.049GluTyr: 1.049 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
4.91PheAla: 4.91 ± 0.089
0.349PheCys: 0.349 ± 0.018
2.588PheAsp: 2.588 ± 0.053
1.91PheGlu: 1.91 ± 0.047
1.206PhePhe: 1.206 ± 0.046
3.614PheGly: 3.614 ± 0.078
0.77PheHis: 0.77 ± 0.028
1.508PheIle: 1.508 ± 0.046
0.889PheLys: 0.889 ± 0.033
2.998PheLeu: 2.998 ± 0.068
0.796PheMet: 0.796 ± 0.031
0.998PheAsn: 0.998 ± 0.036
1.547PhePro: 1.547 ± 0.042
0.899PheGln: 0.899 ± 0.035
2.132PheArg: 2.132 ± 0.049
2.056PheSer: 2.056 ± 0.047
1.946PheThr: 1.946 ± 0.051
2.468PheVal: 2.468 ± 0.061
0.546PheTrp: 0.546 ± 0.028
0.92PheTyr: 0.92 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
9.932GlyAla: 9.932 ± 0.125
0.881GlyCys: 0.881 ± 0.036
4.702GlyAsp: 4.702 ± 0.084
5.461GlyGlu: 5.461 ± 0.091
3.552GlyPhe: 3.552 ± 0.069
8.434GlyGly: 8.434 ± 0.15
1.871GlyHis: 1.871 ± 0.051
4.561GlyIle: 4.561 ± 0.142
4.036GlyLys: 4.036 ± 0.087
9.167GlyLeu: 9.167 ± 0.105
2.685GlyMet: 2.685 ± 0.067
2.633GlyAsn: 2.633 ± 0.13
3.888GlyPro: 3.888 ± 0.069
3.133GlyGln: 3.133 ± 0.059
6.22GlyArg: 6.22 ± 0.094
5.148GlySer: 5.148 ± 0.119
5.158GlyThr: 5.158 ± 0.149
6.205GlyVal: 6.205 ± 0.092
1.718GlyTrp: 1.718 ± 0.044
2.286GlyTyr: 2.286 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.432HisAla: 2.432 ± 0.061
0.263HisCys: 0.263 ± 0.017
1.112HisAsp: 1.112 ± 0.036
1.038HisGlu: 1.038 ± 0.037
0.899HisPhe: 0.899 ± 0.031
2.026HisGly: 2.026 ± 0.059
0.561HisHis: 0.561 ± 0.033
0.849HisIle: 0.849 ± 0.032
0.535HisLys: 0.535 ± 0.026
1.921HisLeu: 1.921 ± 0.046
0.465HisMet: 0.465 ± 0.022
0.47HisAsn: 0.47 ± 0.023
1.314HisPro: 1.314 ± 0.039
0.525HisGln: 0.525 ± 0.023
1.462HisArg: 1.462 ± 0.043
0.984HisSer: 0.984 ± 0.031
0.748HisThr: 0.748 ± 0.029
1.474HisVal: 1.474 ± 0.044
0.333HisTrp: 0.333 ± 0.018
0.582HisTyr: 0.582 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
7.64IleAla: 7.64 ± 0.104
0.478IleCys: 0.478 ± 0.024
3.662IleAsp: 3.662 ± 0.065
3.433IleGlu: 3.433 ± 0.061
1.341IlePhe: 1.341 ± 0.038
4.948IleGly: 4.948 ± 0.071
0.891IleHis: 0.891 ± 0.03
1.796IleIle: 1.796 ± 0.044
1.261IleLys: 1.261 ± 0.037
3.44IleLeu: 3.44 ± 0.071
0.892IleMet: 0.892 ± 0.032
1.372IleAsn: 1.372 ± 0.049
2.173IlePro: 2.173 ± 0.058
0.987IleGln: 0.987 ± 0.031
2.898IleArg: 2.898 ± 0.048
2.577IleSer: 2.577 ± 0.072
2.631IleThr: 2.631 ± 0.074
3.725IleVal: 3.725 ± 0.067
0.568IleTrp: 0.568 ± 0.028
0.967IleTyr: 0.967 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.731LysAla: 4.731 ± 0.089
0.189LysCys: 0.189 ± 0.016
1.724LysAsp: 1.724 ± 0.044
1.515LysGlu: 1.515 ± 0.048
0.896LysPhe: 0.896 ± 0.038
3.107LysGly: 3.107 ± 0.064
0.579LysHis: 0.579 ± 0.025
1.484LysIle: 1.484 ± 0.044
1.156LysLys: 1.156 ± 0.048
3.382LysLeu: 3.382 ± 0.066
0.806LysMet: 0.806 ± 0.029
0.735LysAsn: 0.735 ± 0.03
2.17LysPro: 2.17 ± 0.063
0.949LysGln: 0.949 ± 0.036
2.247LysArg: 2.247 ± 0.054
1.635LysSer: 1.635 ± 0.045
1.564LysThr: 1.564 ± 0.044
2.727LysVal: 2.727 ± 0.058
0.489LysTrp: 0.489 ± 0.022
0.647LysTyr: 0.647 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
15.289LeuAla: 15.289 ± 0.161
0.875LeuCys: 0.875 ± 0.033
5.757LeuAsp: 5.757 ± 0.09
5.125LeuGlu: 5.125 ± 0.084
3.496LeuPhe: 3.496 ± 0.075
8.712LeuGly: 8.712 ± 0.106
1.938LeuHis: 1.938 ± 0.053
4.229LeuIle: 4.229 ± 0.085
3.198LeuLys: 3.198 ± 0.069
9.509LeuLeu: 9.509 ± 0.149
2.11LeuMet: 2.11 ± 0.051
2.375LeuAsn: 2.375 ± 0.052
5.816LeuPro: 5.816 ± 0.092
2.433LeuGln: 2.433 ± 0.061
6.738LeuArg: 6.738 ± 0.098
6.058LeuSer: 6.058 ± 0.09
5.02LeuThr: 5.02 ± 0.073
7.593LeuVal: 7.593 ± 0.098
1.286LeuTrp: 1.286 ± 0.044
1.859LeuTyr: 1.859 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
3.65MetAla: 3.65 ± 0.073
0.186MetCys: 0.186 ± 0.015
1.214MetAsp: 1.214 ± 0.039
1.206MetGlu: 1.206 ± 0.034
0.661MetPhe: 0.661 ± 0.026
2.154MetGly: 2.154 ± 0.052
0.448MetHis: 0.448 ± 0.025
1.279MetIle: 1.279 ± 0.04
1.038MetLys: 1.038 ± 0.033
2.611MetLeu: 2.611 ± 0.057
0.664MetMet: 0.664 ± 0.031
0.7MetAsn: 0.7 ± 0.028
1.53MetPro: 1.53 ± 0.049
0.86MetGln: 0.86 ± 0.029
1.806MetArg: 1.806 ± 0.042
1.493MetSer: 1.493 ± 0.043
1.642MetThr: 1.642 ± 0.041
1.853MetVal: 1.853 ± 0.046
0.276MetTrp: 0.276 ± 0.019
0.25MetTyr: 0.25 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.298AsnAla: 3.298 ± 0.073
0.28AsnCys: 0.28 ± 0.02
1.314AsnAsp: 1.314 ± 0.037
1.14AsnGlu: 1.14 ± 0.034
0.985AsnPhe: 0.985 ± 0.039
2.538AsnGly: 2.538 ± 0.116
0.467AsnHis: 0.467 ± 0.02
1.256AsnIle: 1.256 ± 0.051
0.675AsnLys: 0.675 ± 0.028
2.56AsnLeu: 2.56 ± 0.06
0.584AsnMet: 0.584 ± 0.027
0.705AsnAsn: 0.705 ± 0.035
1.94AsnPro: 1.94 ± 0.057
0.803AsnGln: 0.803 ± 0.029
1.883AsnArg: 1.883 ± 0.046
1.282AsnSer: 1.282 ± 0.053
1.257AsnThr: 1.257 ± 0.061
1.948AsnVal: 1.948 ± 0.08
0.438AsnTrp: 0.438 ± 0.02
0.732AsnTyr: 0.732 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
7.545ProAla: 7.545 ± 0.117
0.388ProCys: 0.388 ± 0.023
3.494ProAsp: 3.494 ± 0.061
3.839ProGlu: 3.839 ± 0.074
1.853ProPhe: 1.853 ± 0.05
5.243ProGly: 5.243 ± 0.081
1.061ProHis: 1.061 ± 0.039
2.18ProIle: 2.18 ± 0.049
1.672ProLys: 1.672 ± 0.043
5.049ProLeu: 5.049 ± 0.082
1.251ProMet: 1.251 ± 0.037
1.225ProAsn: 1.225 ± 0.038
2.751ProPro: 2.751 ± 0.089
1.998ProGln: 1.998 ± 0.044
3.16ProArg: 3.16 ± 0.068
2.708ProSer: 2.708 ± 0.058
2.229ProThr: 2.229 ± 0.053
4.497ProVal: 4.497 ± 0.087
0.737ProTrp: 0.737 ± 0.03
1.021ProTyr: 1.021 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
4.436GlnAla: 4.436 ± 0.079
0.266GlnCys: 0.266 ± 0.018
1.353GlnAsp: 1.353 ± 0.037
1.353GlnGlu: 1.353 ± 0.043
1.173GlnPhe: 1.173 ± 0.041
2.702GlnGly: 2.702 ± 0.062
0.622GlnHis: 0.622 ± 0.029
1.571GlnIle: 1.571 ± 0.043
0.943GlnLys: 0.943 ± 0.032
3.094GlnLeu: 3.094 ± 0.057
0.861GlnMet: 0.861 ± 0.03
0.698GlnAsn: 0.698 ± 0.025
1.917GlnPro: 1.917 ± 0.052
1.137GlnGln: 1.137 ± 0.041
2.342GlnArg: 2.342 ± 0.056
1.66GlnSer: 1.66 ± 0.049
1.486GlnThr: 1.486 ± 0.049
2.549GlnVal: 2.549 ± 0.051
0.521GlnTrp: 0.521 ± 0.022
0.606GlnTyr: 0.606 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
8.129ArgAla: 8.129 ± 0.113
0.544ArgCys: 0.544 ± 0.027
3.875ArgAsp: 3.875 ± 0.077
4.28ArgGlu: 4.28 ± 0.072
3.022ArgPhe: 3.022 ± 0.059
5.101ArgGly: 5.101 ± 0.072
1.69ArgHis: 1.69 ± 0.05
4.009ArgIle: 4.009 ± 0.072
2.376ArgLys: 2.376 ± 0.058
8.107ArgLeu: 8.107 ± 0.124
2.021ArgMet: 2.021 ± 0.043
1.862ArgAsn: 1.862 ± 0.047
3.578ArgPro: 3.578 ± 0.07
2.63ArgGln: 2.63 ± 0.066
5.356ArgArg: 5.356 ± 0.099
3.444ArgSer: 3.444 ± 0.066
3.233ArgThr: 3.233 ± 0.061
4.735ArgVal: 4.735 ± 0.078
1.151ArgTrp: 1.151 ± 0.041
1.612ArgTyr: 1.612 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.68SerAla: 6.68 ± 0.089
0.489SerCys: 0.489 ± 0.027
2.747SerAsp: 2.747 ± 0.054
2.695SerGlu: 2.695 ± 0.058
1.943SerPhe: 1.943 ± 0.052
5.822SerGly: 5.822 ± 0.166
0.96SerHis: 0.96 ± 0.035
2.458SerIle: 2.458 ± 0.059
1.477SerLys: 1.477 ± 0.038
5.477SerLeu: 5.477 ± 0.09
1.266SerMet: 1.266 ± 0.044
1.399SerAsn: 1.399 ± 0.058
2.972SerPro: 2.972 ± 0.055
1.668SerGln: 1.668 ± 0.043
3.68SerArg: 3.68 ± 0.065
2.831SerSer: 2.831 ± 0.081
2.52SerThr: 2.52 ± 0.086
3.632SerVal: 3.632 ± 0.071
0.77SerTrp: 0.77 ± 0.034
1.263SerTyr: 1.263 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
5.92ThrAla: 5.92 ± 0.111
0.502ThrCys: 0.502 ± 0.027
2.478ThrAsp: 2.478 ± 0.06
2.313ThrGlu: 2.313 ± 0.049
1.811ThrPhe: 1.811 ± 0.045
5.561ThrGly: 5.561 ± 0.2
0.907ThrHis: 0.907 ± 0.03
2.759ThrIle: 2.759 ± 0.069
1.254ThrLys: 1.254 ± 0.038
5.095ThrLeu: 5.095 ± 0.073
1.227ThrMet: 1.227 ± 0.036
1.351ThrAsn: 1.351 ± 0.066
2.962ThrPro: 2.962 ± 0.058
1.333ThrGln: 1.333 ± 0.043
3.238ThrArg: 3.238 ± 0.063
2.637ThrSer: 2.637 ± 0.08
2.55ThrThr: 2.55 ± 0.092
4.042ThrVal: 4.042 ± 0.108
0.653ThrTrp: 0.653 ± 0.025
1.165ThrTyr: 1.165 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
9.033ValAla: 9.033 ± 0.118
0.61ValCys: 0.61 ± 0.025
4.108ValAsp: 4.108 ± 0.069
4.494ValGlu: 4.494 ± 0.06
2.318ValPhe: 2.318 ± 0.056
5.303ValGly: 5.303 ± 0.088
1.419ValHis: 1.419 ± 0.043
3.956ValIle: 3.956 ± 0.063
2.253ValLys: 2.253 ± 0.049
7.274ValLeu: 7.274 ± 0.101
1.718ValMet: 1.718 ± 0.055
2.176ValAsn: 2.176 ± 0.062
4.153ValPro: 4.153 ± 0.075
2.046ValGln: 2.046 ± 0.044
4.91ValArg: 4.91 ± 0.074
4.209ValSer: 4.209 ± 0.085
4.507ValThr: 4.507 ± 0.135
5.314ValVal: 5.314 ± 0.078
0.957ValTrp: 0.957 ± 0.031
1.335ValTyr: 1.335 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.397TrpAla: 1.397 ± 0.037
0.142TrpCys: 0.142 ± 0.013
0.736TrpAsp: 0.736 ± 0.026
0.649TrpGlu: 0.649 ± 0.025
0.621TrpPhe: 0.621 ± 0.027
1.05TrpGly: 1.05 ± 0.033
0.389TrpHis: 0.389 ± 0.02
0.739TrpIle: 0.739 ± 0.029
0.549TrpLys: 0.549 ± 0.026
1.871TrpLeu: 1.871 ± 0.052
0.385TrpMet: 0.385 ± 0.022
0.489TrpAsn: 0.489 ± 0.024
0.791TrpPro: 0.791 ± 0.026
0.718TrpGln: 0.718 ± 0.027
1.318TrpArg: 1.318 ± 0.039
0.905TrpSer: 0.905 ± 0.036
0.767TrpThr: 0.767 ± 0.03
0.887TrpVal: 0.887 ± 0.031
0.296TrpTrp: 0.296 ± 0.021
0.309TrpTyr: 0.309 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.472TyrAla: 2.472 ± 0.05
0.235TyrCys: 0.235 ± 0.017
1.401TyrAsp: 1.401 ± 0.049
1.19TyrGlu: 1.19 ± 0.035
0.857TyrPhe: 0.857 ± 0.029
2.063TyrGly: 2.063 ± 0.052
0.516TyrHis: 0.516 ± 0.023
0.798TyrIle: 0.798 ± 0.03
0.642TyrLys: 0.642 ± 0.029
1.999TyrLeu: 1.999 ± 0.047
0.409TyrMet: 0.409 ± 0.019
0.646TyrAsn: 0.646 ± 0.03
1.084TyrPro: 1.084 ± 0.04
0.64TyrGln: 0.64 ± 0.029
1.744TyrArg: 1.744 ± 0.049
1.21TyrSer: 1.21 ± 0.037
0.993TyrThr: 0.993 ± 0.034
1.567TyrVal: 1.567 ± 0.042
0.337TyrTrp: 0.337 ± 0.019
0.603TyrTyr: 0.603 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2854 proteins (910234 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski