Pedobacter duraquae
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4663 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V3C343|A0A4V3C343_9SPHI tRNA-specific 2-thiouridylase MnmA OS=Pedobacter duraquae OX=425511 GN=mnmA PE=3 SV=1
MM1 pKa = 8.01 EE2 pKa = 6.27 DD3 pKa = 3.13 NNITVSVQNPDD14 pKa = 3.0 GTLTALEE21 pKa = 4.75 APVDD25 pKa = 3.68 MGLSLMEE32 pKa = 3.89 FLKK35 pKa = 10.92 ACEE38 pKa = 3.8 YY39 pKa = 10.98 DD40 pKa = 3.51 ILATCGGMALCATCCVDD57 pKa = 3.54 VLEE60 pKa = 5.31 GEE62 pKa = 4.35 EE63 pKa = 4.53 KK64 pKa = 10.73 LNEE67 pKa = 4.07 MSDD70 pKa = 3.95 DD71 pKa = 3.99 EE72 pKa = 4.73 YY73 pKa = 11.97 AMLDD77 pKa = 3.8 TLPDD81 pKa = 4.06 LLPNSRR87 pKa = 11.84 LACQLRR93 pKa = 11.84 LSPEE97 pKa = 3.59 MDD99 pKa = 3.16 GLVVKK104 pKa = 9.93 LHH106 pKa = 6.6 GEE108 pKa = 3.91
Molecular weight: 11.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.541
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.935
Wikipedia 3.643
Rodwell 3.567
Grimsley 3.452
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.012
Thurlkill 3.592
EMBOSS 3.643
Sillero 3.846
Patrickios 0.54
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.831
Protein with the highest isoelectric point:
>tr|A0A4R6II27|A0A4R6II27_9SPHI ATP synthase subunit delta OS=Pedobacter duraquae OX=425511 GN=atpH PE=3 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.39 KK6 pKa = 9.62 RR7 pKa = 11.84 KK8 pKa = 6.72 RR9 pKa = 11.84 HH10 pKa = 5.17 KK11 pKa = 10.05 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4663
0
4663
1620024
29
3096
347.4
38.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.722 ± 0.042
0.732 ± 0.01
5.328 ± 0.024
5.453 ± 0.034
4.983 ± 0.031
6.802 ± 0.033
1.78 ± 0.016
7.342 ± 0.034
6.927 ± 0.034
9.741 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.305 ± 0.017
5.518 ± 0.031
3.713 ± 0.019
3.762 ± 0.023
3.908 ± 0.021
6.489 ± 0.028
5.978 ± 0.044
6.363 ± 0.025
1.092 ± 0.013
4.063 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here