Streptococcus satellite phage Javan361
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZR17|A0A4D5ZR17_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan361 OX=2558661 GN=JavanS361_0014 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.09 KK3 pKa = 10.55 DD4 pKa = 3.62 EE5 pKa = 4.23 IKK7 pKa = 10.61 KK8 pKa = 10.17 YY9 pKa = 11.05 LEE11 pKa = 3.9 TDD13 pKa = 3.3 LEE15 pKa = 4.63 FNVNGRR21 pKa = 11.84 GACFLSSVCVVGYY34 pKa = 10.22 DD35 pKa = 4.18 YY36 pKa = 11.31 EE37 pKa = 4.51 GQQFNTIDD45 pKa = 3.33 EE46 pKa = 4.29 AMEE49 pKa = 4.12 AKK51 pKa = 10.69 VFDD54 pKa = 4.41 GKK56 pKa = 11.11 SLVDD60 pKa = 2.93 IWDD63 pKa = 3.46 EE64 pKa = 4.12 VFPQVSS70 pKa = 3.07
Molecular weight: 7.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.318
IPC2_protein 4.279
IPC_protein 4.151
Toseland 3.961
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.024
Rodwell 3.986
Grimsley 3.884
Solomon 4.101
Lehninger 4.062
Nozaki 4.24
DTASelect 4.406
Thurlkill 3.999
EMBOSS 4.037
Sillero 4.266
Patrickios 3.592
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.191
Protein with the highest isoelectric point:
>tr|A0A4D5ZLJ8|A0A4D5ZLJ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan361 OX=2558661 GN=JavanS361_0005 PE=4 SV=1
MM1 pKa = 7.86 LITSTQAKK9 pKa = 9.74 AIRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.66 QADD17 pKa = 3.4 KK18 pKa = 11.27 KK19 pKa = 10.16 LTAKK23 pKa = 10.18 QAGEE27 pKa = 4.41 EE28 pKa = 3.96 IGVTQVTYY36 pKa = 10.88 RR37 pKa = 11.84 KK38 pKa = 9.3 IRR40 pKa = 11.84 DD41 pKa = 3.51 GGEE44 pKa = 4.09 VKK46 pKa = 10.52 PSIYY50 pKa = 9.91 QKK52 pKa = 11.14 AMQWLAEE59 pKa = 4.48 DD60 pKa = 4.07 YY61 pKa = 11.23
Molecular weight: 6.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.984
IPC2_protein 9.18
IPC_protein 9.136
Toseland 9.955
ProMoST 9.619
Dawson 10.131
Bjellqvist 9.75
Wikipedia 10.277
Rodwell 10.687
Grimsley 10.189
Solomon 10.175
Lehninger 10.145
Nozaki 9.911
DTASelect 9.75
Thurlkill 9.984
EMBOSS 10.35
Sillero 10.028
Patrickios 10.452
IPC_peptide 10.175
IPC2_peptide 8.185
IPC2.peptide.svr19 8.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2547
40
484
141.5
16.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.34 ± 0.411
0.51 ± 0.104
6.125 ± 0.504
9.541 ± 0.896
4.515 ± 0.598
4.319 ± 0.268
1.61 ± 0.32
6.203 ± 0.399
11.15 ± 0.495
9.737 ± 0.609
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.316 ± 0.282
4.829 ± 0.47
2.316 ± 0.254
3.887 ± 0.481
5.065 ± 0.397
5.536 ± 0.385
6.203 ± 0.529
4.947 ± 0.401
0.942 ± 0.134
4.908 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here