Aidingimonas halophila
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3626 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3AYB2|A0A1H3AYB2_9GAMM UPF0250 protein SAMN05443545_10568 OS=Aidingimonas halophila OX=574349 GN=SAMN05443545_10568 PE=3 SV=1
MM1 pKa = 7.22 IANVAIYY8 pKa = 8.94 KK9 pKa = 9.03 WCLITFSILWLAGCSDD25 pKa = 3.76 QSWRR29 pKa = 11.84 TTSISDD35 pKa = 3.76 IMPDD39 pKa = 3.52 LDD41 pKa = 4.71 FEE43 pKa = 4.94 LVDD46 pKa = 3.96 EE47 pKa = 4.77 NGEE50 pKa = 4.04 AVTDD54 pKa = 4.29 DD55 pKa = 4.53 EE56 pKa = 4.9 YY57 pKa = 11.63 LGNPTLLYY65 pKa = 10.68 FGFTYY70 pKa = 10.76 CPDD73 pKa = 3.26 VCPNTLSRR81 pKa = 11.84 LATAIGQLDD90 pKa = 3.77 AEE92 pKa = 4.5 VRR94 pKa = 11.84 DD95 pKa = 4.63 DD96 pKa = 3.47 IRR98 pKa = 11.84 VLFVSVDD105 pKa = 3.54 PGRR108 pKa = 11.84 DD109 pKa = 3.36 TPEE112 pKa = 3.62 VLSRR116 pKa = 11.84 YY117 pKa = 7.68 TDD119 pKa = 2.92 AFGPQFIGLSGDD131 pKa = 3.31 NSALDD136 pKa = 3.8 AVTKK140 pKa = 10.1 RR141 pKa = 11.84 YY142 pKa = 9.64 RR143 pKa = 11.84 VTYY146 pKa = 10.14 SYY148 pKa = 11.67 GEE150 pKa = 4.0 EE151 pKa = 4.38 DD152 pKa = 3.07 SDD154 pKa = 3.9 GNYY157 pKa = 10.92 DD158 pKa = 3.65 VTHH161 pKa = 6.69 SSAVFAFDD169 pKa = 4.26 QNGEE173 pKa = 4.07 AQFMIRR179 pKa = 11.84 DD180 pKa = 3.94 TDD182 pKa = 3.75 SMQDD186 pKa = 2.97 IVSDD190 pKa = 4.29 LDD192 pKa = 3.33 QLVAEE197 pKa = 4.72 EE198 pKa = 4.2
Molecular weight: 22.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.389
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.834
Patrickios 1.1
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A1H2XSR1|A0A1H2XSR1_9GAMM L-threonine dehydratase OS=Aidingimonas halophila OX=574349 GN=ilvA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.2 RR12 pKa = 11.84 KK13 pKa = 9.69 RR14 pKa = 11.84 MHH16 pKa = 6.75 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3626
0
3626
1177209
29
8694
324.7
35.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.289 ± 0.042
0.983 ± 0.016
6.389 ± 0.055
6.48 ± 0.044
3.447 ± 0.029
8.04 ± 0.055
2.592 ± 0.03
5.082 ± 0.033
2.545 ± 0.035
11.102 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.641 ± 0.02
2.676 ± 0.021
4.788 ± 0.034
3.819 ± 0.027
7.074 ± 0.046
5.804 ± 0.036
5.185 ± 0.029
7.096 ± 0.038
1.485 ± 0.021
2.482 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here