Amino acid dipepetide frequency for Nitrosomonas sp. (strain Is79A3)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.32AlaAla: 9.32 ± 0.14	1.0AlaCys: 1.0 ± 0.033	4.683AlaAsp: 4.683 ± 0.064	5.641AlaGlu: 5.641 ± 0.083	3.27AlaPhe: 3.27 ± 0.056	6.561AlaGly: 6.561 ± 0.106	1.986AlaHis: 1.986 ± 0.049	6.456AlaIle: 6.456 ± 0.088	4.453AlaLys: 4.453 ± 0.09	9.673AlaLeu: 9.673 ± 0.131	2.378AlaMet: 2.378 ± 0.05	3.427AlaAsn: 3.427 ± 0.068	3.026AlaPro: 3.026 ± 0.069	3.862AlaGln: 3.862 ± 0.075	4.766AlaArg: 4.766 ± 0.086	5.241AlaSer: 5.241 ± 0.085	4.546AlaThr: 4.546 ± 0.083	5.892AlaVal: 5.892 ± 0.09	1.136AlaTrp: 1.136 ± 0.043	2.383AlaTyr: 2.383 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.905CysAla: 0.905 ± 0.034	0.167CysCys: 0.167 ± 0.013	0.586CysAsp: 0.586 ± 0.025	0.568CysGlu: 0.568 ± 0.025	0.431CysPhe: 0.431 ± 0.025	0.878CysGly: 0.878 ± 0.034	0.372CysHis: 0.372 ± 0.024	0.634CysIle: 0.634 ± 0.026	0.446CysLys: 0.446 ± 0.025	0.958CysLeu: 0.958 ± 0.031	0.244CysMet: 0.244 ± 0.016	0.418CysAsn: 0.418 ± 0.024	0.451CysPro: 0.451 ± 0.022	0.376CysGln: 0.376 ± 0.018	0.547CysArg: 0.547 ± 0.022	0.54CysSer: 0.54 ± 0.027	0.501CysThr: 0.501 ± 0.027	0.658CysVal: 0.658 ± 0.025	0.125CysTrp: 0.125 ± 0.011	0.321CysTyr: 0.321 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.524AspAla: 4.524 ± 0.068	0.541AspCys: 0.541 ± 0.024	2.75AspAsp: 2.75 ± 0.061	3.532AspGlu: 3.532 ± 0.071	2.35AspPhe: 2.35 ± 0.047	3.67AspGly: 3.67 ± 0.068	1.292AspHis: 1.292 ± 0.042	3.662AspIle: 3.662 ± 0.066	2.853AspLys: 2.853 ± 0.058	5.214AspLeu: 5.214 ± 0.077	1.257AspMet: 1.257 ± 0.036	2.117AspAsn: 2.117 ± 0.046	2.461AspPro: 2.461 ± 0.05	2.141AspGln: 2.141 ± 0.05	2.657AspArg: 2.657 ± 0.055	3.239AspSer: 3.239 ± 0.058	2.884AspThr: 2.884 ± 0.06	3.222AspVal: 3.222 ± 0.061	0.897AspTrp: 0.897 ± 0.031	1.884AspTyr: 1.884 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	5.191GluAla: 5.191 ± 0.086	0.506GluCys: 0.506 ± 0.025	2.653GluAsp: 2.653 ± 0.059	3.41GluGlu: 3.41 ± 0.074	2.381GluPhe: 2.381 ± 0.054	3.164GluGly: 3.164 ± 0.052	1.411GluHis: 1.411 ± 0.036	5.045GluIle: 5.045 ± 0.077	3.973GluLys: 3.973 ± 0.081	6.358GluLeu: 6.358 ± 0.087	1.605GluMet: 1.605 ± 0.043	2.786GluAsn: 2.786 ± 0.047	2.043GluPro: 2.043 ± 0.051	3.082GluGln: 3.082 ± 0.063	3.586GluArg: 3.586 ± 0.073	3.725GluSer: 3.725 ± 0.068	3.311GluThr: 3.311 ± 0.059	3.575GluVal: 3.575 ± 0.061	0.785GluTrp: 0.785 ± 0.029	1.604GluTyr: 1.604 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	3.42PheAla: 3.42 ± 0.059	0.484PheCys: 0.484 ± 0.024	2.623PheAsp: 2.623 ± 0.05	2.327PheGlu: 2.327 ± 0.044	1.823PhePhe: 1.823 ± 0.047	2.955PheGly: 2.955 ± 0.062	0.851PheHis: 0.851 ± 0.031	2.69PheIle: 2.69 ± 0.06	1.723PheLys: 1.723 ± 0.037	3.844PheLeu: 3.844 ± 0.067	0.97PheMet: 0.97 ± 0.03	1.863PheAsn: 1.863 ± 0.045	1.708PhePro: 1.708 ± 0.046	1.334PheGln: 1.334 ± 0.041	1.911PheArg: 1.911 ± 0.047	3.14PheSer: 3.14 ± 0.061	2.287PheThr: 2.287 ± 0.056	2.539PheVal: 2.539 ± 0.055	0.58PheTrp: 0.58 ± 0.027	1.289PheTyr: 1.289 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	5.346GlyAla: 5.346 ± 0.087	0.872GlyCys: 0.872 ± 0.032	3.462GlyAsp: 3.462 ± 0.062	3.849GlyGlu: 3.849 ± 0.059	3.185GlyPhe: 3.185 ± 0.056	5.09GlyGly: 5.09 ± 0.116	1.733GlyHis: 1.733 ± 0.04	5.284GlyIle: 5.284 ± 0.079	4.219GlyLys: 4.219 ± 0.07	6.824GlyLeu: 6.824 ± 0.087	1.935GlyMet: 1.935 ± 0.05	2.962GlyAsn: 2.962 ± 0.065	1.746GlyPro: 1.746 ± 0.046	2.485GlyGln: 2.485 ± 0.056	3.411GlyArg: 3.411 ± 0.067	4.223GlySer: 4.223 ± 0.077	3.716GlyThr: 3.716 ± 0.077	4.579GlyVal: 4.579 ± 0.077	1.049GlyTrp: 1.049 ± 0.037	2.385GlyTyr: 2.385 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.198HisAla: 2.198 ± 0.054	0.336HisCys: 0.336 ± 0.019	1.275HisAsp: 1.275 ± 0.039	1.33HisGlu: 1.33 ± 0.035	1.133HisPhe: 1.133 ± 0.036	1.775HisGly: 1.775 ± 0.049	0.779HisHis: 0.779 ± 0.028	1.522HisIle: 1.522 ± 0.039	1.01HisLys: 1.01 ± 0.033	2.447HisLeu: 2.447 ± 0.05	0.507HisMet: 0.507 ± 0.022	0.902HisAsn: 0.902 ± 0.029	1.318HisPro: 1.318 ± 0.041	1.122HisGln: 1.122 ± 0.035	1.208HisArg: 1.208 ± 0.034	1.364HisSer: 1.364 ± 0.038	1.235HisThr: 1.235 ± 0.04	1.441HisVal: 1.441 ± 0.038	0.364HisTrp: 0.364 ± 0.018	0.882HisTyr: 0.882 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.952IleAla: 6.952 ± 0.094	0.712IleCys: 0.712 ± 0.026	4.274IleAsp: 4.274 ± 0.078	4.751IleGlu: 4.751 ± 0.076	2.486IlePhe: 2.486 ± 0.057	4.965IleGly: 4.965 ± 0.078	1.62IleHis: 1.62 ± 0.038	4.298IleIle: 4.298 ± 0.075	3.729IleLys: 3.729 ± 0.076	6.502IleLeu: 6.502 ± 0.097	1.372IleMet: 1.372 ± 0.038	3.211IleAsn: 3.211 ± 0.06	3.309IlePro: 3.309 ± 0.055	2.584IleGln: 2.584 ± 0.053	3.543IleArg: 3.543 ± 0.061	4.63IleSer: 4.63 ± 0.086	4.271IleThr: 4.271 ± 0.079	4.207IleVal: 4.207 ± 0.068	0.701IleTrp: 0.701 ± 0.03	1.897IleTyr: 1.897 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	4.095LysAla: 4.095 ± 0.081	0.356LysCys: 0.356 ± 0.019	2.526LysAsp: 2.526 ± 0.055	3.254LysGlu: 3.254 ± 0.061	1.664LysPhe: 1.664 ± 0.042	2.767LysGly: 2.767 ± 0.053	1.218LysHis: 1.218 ± 0.037	3.876LysIle: 3.876 ± 0.064	3.149LysLys: 3.149 ± 0.07	5.489LysLeu: 5.489 ± 0.081	1.314LysMet: 1.314 ± 0.041	2.598LysAsn: 2.598 ± 0.059	2.404LysPro: 2.404 ± 0.048	2.813LysGln: 2.813 ± 0.064	2.982LysArg: 2.982 ± 0.067	3.148LysSer: 3.148 ± 0.062	2.945LysThr: 2.945 ± 0.05	2.918LysVal: 2.918 ± 0.062	0.57LysTrp: 0.57 ± 0.027	1.277LysTyr: 1.277 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	9.88LeuAla: 9.88 ± 0.124	1.05LeuCys: 1.05 ± 0.035	5.567LeuAsp: 5.567 ± 0.073	5.969LeuGlu: 5.969 ± 0.091	4.104LeuPhe: 4.104 ± 0.068	6.668LeuGly: 6.668 ± 0.083	2.42LeuHis: 2.42 ± 0.05	7.047LeuIle: 7.047 ± 0.107	5.281LeuLys: 5.281 ± 0.08	11.4LeuLeu: 11.4 ± 0.15	2.421LeuMet: 2.421 ± 0.049	4.431LeuAsn: 4.431 ± 0.072	5.112LeuPro: 5.112 ± 0.085	4.38LeuGln: 4.38 ± 0.08	5.858LeuArg: 5.858 ± 0.079	7.161LeuSer: 7.161 ± 0.097	5.931LeuThr: 5.931 ± 0.101	6.256LeuVal: 6.256 ± 0.083	1.169LeuTrp: 1.169 ± 0.039	2.554LeuTyr: 2.554 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.161MetAla: 2.161 ± 0.058	0.169MetCys: 0.169 ± 0.012	1.169MetAsp: 1.169 ± 0.035	1.301MetGlu: 1.301 ± 0.035	0.744MetPhe: 0.744 ± 0.029	1.555MetGly: 1.555 ± 0.043	0.661MetHis: 0.661 ± 0.027	1.563MetIle: 1.563 ± 0.039	1.388MetLys: 1.388 ± 0.036	2.698MetLeu: 2.698 ± 0.056	0.618MetMet: 0.618 ± 0.027	1.131MetAsn: 1.131 ± 0.032	1.236MetPro: 1.236 ± 0.039	1.238MetGln: 1.238 ± 0.034	1.444MetArg: 1.444 ± 0.037	1.525MetSer: 1.525 ± 0.041	1.421MetThr: 1.421 ± 0.041	1.532MetVal: 1.532 ± 0.045	0.177MetTrp: 0.177 ± 0.014	0.419MetTyr: 0.419 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.535AsnAla: 3.535 ± 0.067	0.403AsnCys: 0.403 ± 0.021	2.28AsnAsp: 2.28 ± 0.059	2.411AsnGlu: 2.411 ± 0.053	1.72AsnPhe: 1.72 ± 0.042	2.933AsnGly: 2.933 ± 0.067	0.989AsnHis: 0.989 ± 0.033	2.914AsnIle: 2.914 ± 0.061	2.255AsnLys: 2.255 ± 0.053	4.54AsnLeu: 4.54 ± 0.075	0.88AsnMet: 0.88 ± 0.028	1.976AsnAsn: 1.976 ± 0.05	2.548AsnPro: 2.548 ± 0.055	2.058AsnGln: 2.058 ± 0.051	2.287AsnArg: 2.287 ± 0.047	2.439AsnSer: 2.439 ± 0.054	2.295AsnThr: 2.295 ± 0.052	2.422AsnVal: 2.422 ± 0.055	0.628AsnTrp: 0.628 ± 0.026	1.279AsnTyr: 1.279 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	3.923ProAla: 3.923 ± 0.064	0.372ProCys: 0.372 ± 0.02	2.846ProAsp: 2.846 ± 0.054	3.486ProGlu: 3.486 ± 0.058	1.783ProPhe: 1.783 ± 0.04	3.192ProGly: 3.192 ± 0.062	0.972ProHis: 0.972 ± 0.034	2.767ProIle: 2.767 ± 0.055	1.818ProLys: 1.818 ± 0.042	4.081ProLeu: 4.081 ± 0.06	0.992ProMet: 0.992 ± 0.032	1.688ProAsn: 1.688 ± 0.038	1.848ProPro: 1.848 ± 0.055	1.721ProGln: 1.721 ± 0.041	1.717ProArg: 1.717 ± 0.049	2.416ProSer: 2.416 ± 0.046	1.898ProThr: 1.898 ± 0.045	3.376ProVal: 3.376 ± 0.066	0.598ProTrp: 0.598 ± 0.025	1.266ProTyr: 1.266 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	4.252GlnAla: 4.252 ± 0.068	0.372GlnCys: 0.372 ± 0.019	1.819GlnAsp: 1.819 ± 0.044	2.414GlnGlu: 2.414 ± 0.06	1.668GlnPhe: 1.668 ± 0.035	2.607GlnGly: 2.607 ± 0.054	1.233GlnHis: 1.233 ± 0.037	3.094GlnIle: 3.094 ± 0.049	2.264GlnLys: 2.264 ± 0.054	4.791GlnLeu: 4.791 ± 0.068	1.004GlnMet: 1.004 ± 0.032	1.741GlnAsn: 1.741 ± 0.045	1.805GlnPro: 1.805 ± 0.046	2.487GlnGln: 2.487 ± 0.059	2.556GlnArg: 2.556 ± 0.055	2.625GlnSer: 2.625 ± 0.06	2.292GlnThr: 2.292 ± 0.049	2.689GlnVal: 2.689 ± 0.055	0.614GlnTrp: 0.614 ± 0.023	1.176GlnTyr: 1.176 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	4.166ArgAla: 4.166 ± 0.074	0.484ArgCys: 0.484 ± 0.024	2.707ArgAsp: 2.707 ± 0.056	3.389ArgGlu: 3.389 ± 0.059	2.407ArgPhe: 2.407 ± 0.047	3.089ArgGly: 3.089 ± 0.058	1.332ArgHis: 1.332 ± 0.044	4.206ArgIle: 4.206 ± 0.067	2.722ArgLys: 2.722 ± 0.059	5.904ArgLeu: 5.904 ± 0.09	1.409ArgMet: 1.409 ± 0.036	2.501ArgAsn: 2.501 ± 0.051	1.892ArgPro: 1.892 ± 0.048	2.333ArgGln: 2.333 ± 0.056	3.119ArgArg: 3.119 ± 0.059	2.935ArgSer: 2.935 ± 0.052	2.533ArgThr: 2.533 ± 0.057	3.241ArgVal: 3.241 ± 0.066	0.828ArgTrp: 0.828 ± 0.032	1.952ArgTyr: 1.952 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	5.551SerAla: 5.551 ± 0.083	0.66SerCys: 0.66 ± 0.028	3.41SerAsp: 3.41 ± 0.066	3.513SerGlu: 3.513 ± 0.074	2.613SerPhe: 2.613 ± 0.053	5.36SerGly: 5.36 ± 0.089	1.48SerHis: 1.48 ± 0.042	4.256SerIle: 4.256 ± 0.07	2.888SerLys: 2.888 ± 0.065	6.235SerLeu: 6.235 ± 0.081	1.539SerMet: 1.539 ± 0.041	2.737SerAsn: 2.737 ± 0.06	2.58SerPro: 2.58 ± 0.051	2.539SerGln: 2.539 ± 0.052	3.029SerArg: 3.029 ± 0.053	4.074SerSer: 4.074 ± 0.085	3.236SerThr: 3.236 ± 0.061	4.028SerVal: 4.028 ± 0.068	0.757SerTrp: 0.757 ± 0.023	1.719SerTyr: 1.719 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.959ThrAla: 4.959 ± 0.077	0.501ThrCys: 0.501 ± 0.022	2.904ThrAsp: 2.904 ± 0.06	3.174ThrGlu: 3.174 ± 0.06	2.046ThrPhe: 2.046 ± 0.047	4.538ThrGly: 4.538 ± 0.092	1.342ThrHis: 1.342 ± 0.038	3.546ThrIle: 3.546 ± 0.065	2.196ThrLys: 2.196 ± 0.049	6.111ThrLeu: 6.111 ± 0.1	1.08ThrMet: 1.08 ± 0.034	2.135ThrAsn: 2.135 ± 0.052	2.82ThrPro: 2.82 ± 0.056	2.375ThrGln: 2.375 ± 0.05	2.635ThrArg: 2.635 ± 0.05	3.149ThrSer: 3.149 ± 0.068	2.992ThrThr: 2.992 ± 0.072	3.71ThrVal: 3.71 ± 0.065	0.65ThrTrp: 0.65 ± 0.029	1.508ThrTyr: 1.508 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	5.82ValAla: 5.82 ± 0.086	0.647ValCys: 0.647 ± 0.027	3.456ValAsp: 3.456 ± 0.063	3.654ValGlu: 3.654 ± 0.065	2.601ValPhe: 2.601 ± 0.055	3.806ValGly: 3.806 ± 0.071	1.327ValHis: 1.327 ± 0.038	4.746ValIle: 4.746 ± 0.066	3.223ValLys: 3.223 ± 0.06	6.656ValLeu: 6.656 ± 0.112	1.785ValMet: 1.785 ± 0.041	2.632ValAsn: 2.632 ± 0.05	2.593ValPro: 2.593 ± 0.055	2.258ValGln: 2.258 ± 0.053	3.169ValArg: 3.169 ± 0.061	4.104ValSer: 4.104 ± 0.071	3.976ValThr: 3.976 ± 0.067	4.404ValVal: 4.404 ± 0.081	0.753ValTrp: 0.753 ± 0.027	1.633ValTyr: 1.633 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.877TrpAla: 0.877 ± 0.033	0.155TrpCys: 0.155 ± 0.013	0.643TrpAsp: 0.643 ± 0.027	0.644TrpGlu: 0.644 ± 0.025	0.564TrpPhe: 0.564 ± 0.022	0.778TrpGly: 0.778 ± 0.031	0.357TrpHis: 0.357 ± 0.018	0.913TrpIle: 0.913 ± 0.032	0.636TrpLys: 0.636 ± 0.023	1.738TrpLeu: 1.738 ± 0.049	0.312TrpMet: 0.312 ± 0.018	0.566TrpAsn: 0.566 ± 0.026	0.48TrpPro: 0.48 ± 0.023	0.796TrpGln: 0.796 ± 0.032	0.835TrpArg: 0.835 ± 0.028	0.739TrpSer: 0.739 ± 0.033	0.562TrpThr: 0.562 ± 0.028	0.868TrpVal: 0.868 ± 0.027	0.206TrpTrp: 0.206 ± 0.016	0.345TrpTyr: 0.345 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.628TyrAla: 2.628 ± 0.055	0.34TyrCys: 0.34 ± 0.019	1.518TyrAsp: 1.518 ± 0.046	1.577TyrGlu: 1.577 ± 0.042	1.384TyrPhe: 1.384 ± 0.034	2.004TyrGly: 2.004 ± 0.049	0.772TyrHis: 0.772 ± 0.032	1.496TyrIle: 1.496 ± 0.047	1.134TyrLys: 1.134 ± 0.036	3.2TyrLeu: 3.2 ± 0.066	0.524TyrMet: 0.524 ± 0.023	0.974TyrAsn: 0.974 ± 0.035	1.378TyrPro: 1.378 ± 0.041	1.52TyrGln: 1.52 ± 0.043	1.89TyrArg: 1.89 ± 0.047	1.787TyrSer: 1.787 ± 0.039	1.539TyrThr: 1.539 ± 0.041	1.686TyrVal: 1.686 ± 0.033	0.424TyrTrp: 0.424 ± 0.02	0.972TyrTyr: 0.972 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3273 proteins (1023771 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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