Cauliflower mosaic virus (strain Strasbourg) (CaMV)
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P03545|MVP_CAMVS Movement protein OS=Cauliflower mosaic virus (strain Strasbourg) OX=10648 GN=ORF I PE=1 SV=1
MM1 pKa = 7.73 AEE3 pKa = 4.5 SILDD7 pKa = 3.48 RR8 pKa = 11.84 TINRR12 pKa = 11.84 FWYY15 pKa = 10.5 NLGEE19 pKa = 4.56 DD20 pKa = 4.62 CLSEE24 pKa = 4.25 SQFDD28 pKa = 3.63 LMIRR32 pKa = 11.84 LMEE35 pKa = 4.36 EE36 pKa = 4.14 SLDD39 pKa = 3.54 GDD41 pKa = 3.95 QIIDD45 pKa = 3.92 LTSLPSDD52 pKa = 3.49 NLQVEE57 pKa = 4.67 QVMTTTEE64 pKa = 3.94 DD65 pKa = 3.63 SISEE69 pKa = 4.18 EE70 pKa = 3.76 EE71 pKa = 4.65 SEE73 pKa = 4.13 FLLAIGEE80 pKa = 4.38 TSEE83 pKa = 4.38 EE84 pKa = 4.14 EE85 pKa = 4.08 SDD87 pKa = 3.74 SGEE90 pKa = 4.0 EE91 pKa = 4.06 PEE93 pKa = 4.39 FEE95 pKa = 4.26 QVRR98 pKa = 11.84 MDD100 pKa = 3.4 RR101 pKa = 11.84 TGGTEE106 pKa = 3.59 IPKK109 pKa = 10.47 EE110 pKa = 3.95 EE111 pKa = 4.82 DD112 pKa = 3.31 GEE114 pKa = 4.73 GPSRR118 pKa = 11.84 YY119 pKa = 9.29 NEE121 pKa = 4.24 RR122 pKa = 11.84 KK123 pKa = 9.81 RR124 pKa = 11.84 KK125 pKa = 7.47 TPEE128 pKa = 3.29 DD129 pKa = 4.14 RR130 pKa = 11.84 YY131 pKa = 10.74 FPTQPKK137 pKa = 8.96 TIPGQKK143 pKa = 6.7 QTSMGMLNIDD153 pKa = 3.55 CQTNRR158 pKa = 11.84 RR159 pKa = 11.84 TLIDD163 pKa = 3.42 DD164 pKa = 4.03 WAAEE168 pKa = 3.64 IGLIVKK174 pKa = 7.4 TNRR177 pKa = 11.84 EE178 pKa = 4.21 DD179 pKa = 3.71 YY180 pKa = 10.97 LDD182 pKa = 4.59 PEE184 pKa = 4.86 TILLLMEE191 pKa = 5.35 HH192 pKa = 6.19 KK193 pKa = 9.65 TSGIAKK199 pKa = 8.99 EE200 pKa = 4.39 LIRR203 pKa = 11.84 NTRR206 pKa = 11.84 WNRR209 pKa = 11.84 TTGDD213 pKa = 3.34 IIEE216 pKa = 4.17 QVIDD220 pKa = 3.41 AMYY223 pKa = 11.1 TMFLGLNYY231 pKa = 10.01 SDD233 pKa = 4.16 NKK235 pKa = 9.76 VAEE238 pKa = 4.95 KK239 pKa = 9.96 IDD241 pKa = 3.71 EE242 pKa = 4.11 QEE244 pKa = 3.87 KK245 pKa = 10.81 AKK247 pKa = 10.57 IRR249 pKa = 11.84 MTKK252 pKa = 10.23 LQLCDD257 pKa = 2.78 ICYY260 pKa = 10.45 LEE262 pKa = 5.71 EE263 pKa = 4.14 FTCDD267 pKa = 3.39 YY268 pKa = 9.76 EE269 pKa = 5.13 KK270 pKa = 11.56 NMYY273 pKa = 8.34 KK274 pKa = 9.48 TEE276 pKa = 4.02 LADD279 pKa = 3.74 FPGYY283 pKa = 10.07 INQYY287 pKa = 9.44 LSKK290 pKa = 10.49 IPIIGEE296 pKa = 3.82 KK297 pKa = 10.07 ALTRR301 pKa = 11.84 FRR303 pKa = 11.84 HH304 pKa = 5.1 EE305 pKa = 4.41 ANGTSIYY312 pKa = 10.98 SLGFAAKK319 pKa = 9.37 IVKK322 pKa = 10.16 EE323 pKa = 4.25 EE324 pKa = 3.98 LSKK327 pKa = 10.93 ICDD330 pKa = 3.44 LSKK333 pKa = 10.64 KK334 pKa = 8.32 QKK336 pKa = 10.13 KK337 pKa = 9.23 LKK339 pKa = 10.43 KK340 pKa = 9.92 FNKK343 pKa = 8.29 KK344 pKa = 9.46 CCSIGEE350 pKa = 4.0 ASTEE354 pKa = 4.1 YY355 pKa = 10.83 GCKK358 pKa = 8.94 KK359 pKa = 9.29 TSTKK363 pKa = 10.25 KK364 pKa = 8.99 YY365 pKa = 8.73 HH366 pKa = 6.26 KK367 pKa = 9.99 KK368 pKa = 10.2 RR369 pKa = 11.84 YY370 pKa = 7.13 KK371 pKa = 10.25 KK372 pKa = 10.09 KK373 pKa = 8.1 YY374 pKa = 9.34 KK375 pKa = 10.21 AYY377 pKa = 9.9 KK378 pKa = 9.42 PYY380 pKa = 10.45 KK381 pKa = 8.96 KK382 pKa = 9.89 KK383 pKa = 10.79 KK384 pKa = 8.5 KK385 pKa = 9.57 FRR387 pKa = 11.84 SGKK390 pKa = 8.01 YY391 pKa = 8.61 FKK393 pKa = 10.48 PKK395 pKa = 9.32 EE396 pKa = 4.19 KK397 pKa = 10.3 KK398 pKa = 9.83 GSKK401 pKa = 9.16 QKK403 pKa = 10.65 YY404 pKa = 7.8 CPKK407 pKa = 10.37 GKK409 pKa = 9.81 KK410 pKa = 9.42 DD411 pKa = 3.64 CRR413 pKa = 11.84 CWICNIEE420 pKa = 3.72 GHH422 pKa = 5.91 YY423 pKa = 11.26 ANEE426 pKa = 4.29 CPNRR430 pKa = 11.84 QSSEE434 pKa = 3.98 KK435 pKa = 10.95 AHH437 pKa = 6.75 ILQQAEE443 pKa = 4.36 KK444 pKa = 10.6 LGLQPIEE451 pKa = 4.03 EE452 pKa = 4.81 PYY454 pKa = 10.96 EE455 pKa = 4.14 GVQEE459 pKa = 4.31 VFILEE464 pKa = 4.23 YY465 pKa = 10.61 KK466 pKa = 10.49 EE467 pKa = 4.56 EE468 pKa = 4.1 EE469 pKa = 4.44 EE470 pKa = 4.21 EE471 pKa = 4.39 TSTEE475 pKa = 4.02 EE476 pKa = 4.06 SDD478 pKa = 4.03 GSSTSEE484 pKa = 5.31 DD485 pKa = 3.43 SDD487 pKa = 3.84 SDD489 pKa = 3.58
Molecular weight: 56.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.181
IPC2_protein 5.296
IPC_protein 5.232
Toseland 5.143
ProMoST 5.283
Dawson 5.181
Bjellqvist 5.321
Wikipedia 5.016
Rodwell 5.105
Grimsley 5.054
Solomon 5.181
Lehninger 5.143
Nozaki 5.296
DTASelect 5.397
Thurlkill 5.13
EMBOSS 5.054
Sillero 5.385
Patrickios 4.139
IPC_peptide 5.194
IPC2_peptide 5.372
IPC2.peptide.svr19 5.422
Protein with the highest isoelectric point:
>sp|P03551|VAP_CAMVS Virion-associated protein OS=Cauliflower mosaic virus (strain Strasbourg) OX=10648 GN=ORF III PE=1 SV=1
MM1 pKa = 7.71 SITGQPHH8 pKa = 6.08 VYY10 pKa = 10.34 KK11 pKa = 10.15 KK12 pKa = 10.26 DD13 pKa = 3.28 TIIRR17 pKa = 11.84 LKK19 pKa = 10.51 PLSLNSNNRR28 pKa = 11.84 SYY30 pKa = 11.62 VFSSSKK36 pKa = 11.2 GNIQNIINHH45 pKa = 6.59 LNNLNEE51 pKa = 3.99 IVGRR55 pKa = 11.84 SLLGIWKK62 pKa = 9.14 INSYY66 pKa = 10.68 FGLSKK71 pKa = 10.89 DD72 pKa = 3.5 PSEE75 pKa = 4.67 SKK77 pKa = 10.77 SKK79 pKa = 10.86 NPSVFNTAKK88 pKa = 10.17 TIFKK92 pKa = 10.49 SGGVDD97 pKa = 3.16 YY98 pKa = 11.05 SSQLKK103 pKa = 9.59 EE104 pKa = 3.87 IKK106 pKa = 10.32 SLLEE110 pKa = 3.97 AQNTRR115 pKa = 11.84 IKK117 pKa = 10.65 SLEE120 pKa = 3.85 KK121 pKa = 10.38 AIQSLEE127 pKa = 3.98 NKK129 pKa = 9.81 IEE131 pKa = 4.09 PEE133 pKa = 4.02 PLTKK137 pKa = 10.77 EE138 pKa = 3.86 EE139 pKa = 4.05 VKK141 pKa = 10.52 EE142 pKa = 3.89 LKK144 pKa = 10.55 EE145 pKa = 4.32 SINSIKK151 pKa = 10.61 EE152 pKa = 3.7 GLKK155 pKa = 10.77 NIIGG159 pKa = 3.69
Molecular weight: 17.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.823
IPC2_protein 8.96
IPC_protein 8.843
Toseland 9.897
ProMoST 9.428
Dawson 10.043
Bjellqvist 9.619
Wikipedia 10.145
Rodwell 10.76
Grimsley 10.072
Solomon 10.058
Lehninger 10.043
Nozaki 9.853
DTASelect 9.619
Thurlkill 9.897
EMBOSS 10.277
Sillero 9.94
Patrickios 10.482
IPC_peptide 10.072
IPC2_peptide 7.936
IPC2.peptide.svr19 7.909
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2303
129
679
383.8
43.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.037 ± 0.604
1.65 ± 0.28
5.124 ± 0.439
8.337 ± 0.941
3.734 ± 0.525
4.993 ± 0.335
2.041 ± 0.405
7.859 ± 0.416
10.812 ± 0.404
8.511 ± 0.574
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.215 ± 0.211
5.862 ± 0.999
4.69 ± 0.746
4.559 ± 0.267
3.517 ± 0.383
7.251 ± 0.764
5.558 ± 0.425
4.342 ± 0.591
0.868 ± 0.165
3.04 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here