Streptomyces pluripotens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6158 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221P2G2|A0A221P2G2_9ACTN RNA polymerase sigma factor OS=Streptomyces pluripotens OX=1355015 GN=LK07_20375 PE=3 SV=1
MM1 pKa = 7.57SYY3 pKa = 11.33DD4 pKa = 3.38LAVWEE9 pKa = 4.81GSKK12 pKa = 10.53PADD15 pKa = 3.36DD16 pKa = 4.64VIAGEE21 pKa = 4.7VLTQLYY27 pKa = 10.44DD28 pKa = 3.69RR29 pKa = 11.84YY30 pKa = 10.45IDD32 pKa = 3.68TEE34 pKa = 4.41EE35 pKa = 4.47EE36 pKa = 4.37FPPSPRR42 pKa = 11.84IAEE45 pKa = 4.19YY46 pKa = 10.9VAGLLARR53 pKa = 11.84WVDD56 pKa = 3.75LTEE59 pKa = 5.21DD60 pKa = 4.21EE61 pKa = 5.09EE62 pKa = 4.72DD63 pKa = 3.43TSPWSTGPLIGEE75 pKa = 3.94ASGPFIYY82 pKa = 10.3FPMRR86 pKa = 11.84YY87 pKa = 10.0SMAEE91 pKa = 3.74EE92 pKa = 3.98ASAYY96 pKa = 10.34AADD99 pKa = 4.57LAASMGLVCYY109 pKa = 10.26DD110 pKa = 3.29PQARR114 pKa = 11.84LLRR117 pKa = 11.84PP118 pKa = 3.46

Molecular weight: 13.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221NZU1|A0A221NZU1_9ACTN Formate hydrogenlyase OS=Streptomyces pluripotens OX=1355015 GN=LK07_16125 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6158

0

6158

2045619

23

7246

332.2

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.355 ± 0.046

0.848 ± 0.01

5.834 ± 0.03

5.623 ± 0.033

2.702 ± 0.019

9.393 ± 0.032

2.443 ± 0.016

3.165 ± 0.023

2.18 ± 0.028

10.355 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.716 ± 0.016

1.76 ± 0.015

6.095 ± 0.029

2.882 ± 0.018

8.18 ± 0.042

5.142 ± 0.029

6.218 ± 0.031

8.509 ± 0.03

1.49 ± 0.012

2.111 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski