Amino acid dipepetide frequency for Streptomyces pluripotens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.296AlaAla: 20.296 ± 0.149
1.152AlaCys: 1.152 ± 0.024
8.017AlaAsp: 8.017 ± 0.073
8.606AlaGlu: 8.606 ± 0.097
3.513AlaPhe: 3.513 ± 0.045
12.823AlaGly: 12.823 ± 0.089
3.016AlaHis: 3.016 ± 0.038
3.426AlaIle: 3.426 ± 0.041
2.881AlaLys: 2.881 ± 0.05
14.191AlaLeu: 14.191 ± 0.103
2.431AlaMet: 2.431 ± 0.031
1.894AlaAsn: 1.894 ± 0.036
7.037AlaPro: 7.037 ± 0.081
3.93AlaGln: 3.93 ± 0.051
10.518AlaArg: 10.518 ± 0.094
5.997AlaSer: 5.997 ± 0.065
6.926AlaThr: 6.926 ± 0.064
12.34AlaVal: 12.34 ± 0.095
1.792AlaTrp: 1.792 ± 0.036
2.761AlaTyr: 2.761 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.15CysAla: 1.15 ± 0.025
0.11CysCys: 0.11 ± 0.009
0.493CysAsp: 0.493 ± 0.016
0.396CysGlu: 0.396 ± 0.014
0.241CysPhe: 0.241 ± 0.012
1.009CysGly: 1.009 ± 0.024
0.212CysHis: 0.212 ± 0.011
0.178CysIle: 0.178 ± 0.009
0.116CysLys: 0.116 ± 0.008
0.801CysLeu: 0.801 ± 0.02
0.123CysMet: 0.123 ± 0.007
0.143CysAsn: 0.143 ± 0.008
0.548CysPro: 0.548 ± 0.017
0.188CysGln: 0.188 ± 0.01
0.672CysArg: 0.672 ± 0.022
0.493CysSer: 0.493 ± 0.015
0.607CysThr: 0.607 ± 0.017
0.718CysVal: 0.718 ± 0.017
0.128CysTrp: 0.128 ± 0.008
0.156CysTyr: 0.156 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.288AspAla: 7.288 ± 0.065
0.435AspCys: 0.435 ± 0.014
3.384AspAsp: 3.384 ± 0.049
3.658AspGlu: 3.658 ± 0.05
1.593AspPhe: 1.593 ± 0.031
6.057AspGly: 6.057 ± 0.058
1.477AspHis: 1.477 ± 0.027
1.86AspIle: 1.86 ± 0.031
1.131AspLys: 1.131 ± 0.029
6.211AspLeu: 6.211 ± 0.068
0.815AspMet: 0.815 ± 0.02
0.967AspAsn: 0.967 ± 0.026
4.335AspPro: 4.335 ± 0.047
1.541AspGln: 1.541 ± 0.029
4.939AspArg: 4.939 ± 0.055
2.552AspSer: 2.552 ± 0.036
3.273AspThr: 3.273 ± 0.039
4.709AspVal: 4.709 ± 0.051
0.987AspTrp: 0.987 ± 0.025
1.128AspTyr: 1.128 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
7.359GluAla: 7.359 ± 0.089
0.384GluCys: 0.384 ± 0.014
2.712GluAsp: 2.712 ± 0.036
3.628GluGlu: 3.628 ± 0.059
1.421GluPhe: 1.421 ± 0.027
4.173GluGly: 4.173 ± 0.05
1.605GluHis: 1.605 ± 0.028
2.179GluIle: 2.179 ± 0.037
1.378GluLys: 1.378 ± 0.032
6.787GluLeu: 6.787 ± 0.07
0.87GluMet: 0.87 ± 0.019
1.016GluAsn: 1.016 ± 0.023
3.345GluPro: 3.345 ± 0.048
2.318GluGln: 2.318 ± 0.039
5.405GluArg: 5.405 ± 0.069
2.516GluSer: 2.516 ± 0.037
2.824GluThr: 2.824 ± 0.041
4.527GluVal: 4.527 ± 0.052
0.701GluTrp: 0.701 ± 0.017
1.082GluTyr: 1.082 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.644PheAla: 3.644 ± 0.048
0.298PheCys: 0.298 ± 0.012
1.877PheAsp: 1.877 ± 0.03
1.421PheGlu: 1.421 ± 0.028
0.886PhePhe: 0.886 ± 0.027
2.992PheGly: 2.992 ± 0.042
0.646PheHis: 0.646 ± 0.019
0.719PheIle: 0.719 ± 0.02
0.505PheLys: 0.505 ± 0.015
2.585PheLeu: 2.585 ± 0.048
0.386PheMet: 0.386 ± 0.015
0.534PheAsn: 0.534 ± 0.017
1.395PhePro: 1.395 ± 0.029
0.67PheGln: 0.67 ± 0.018
1.844PheArg: 1.844 ± 0.028
1.459PheSer: 1.459 ± 0.027
2.013PheThr: 2.013 ± 0.033
2.195PheVal: 2.195 ± 0.034
0.398PheTrp: 0.398 ± 0.013
0.547PheTyr: 0.547 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
10.757GlyAla: 10.757 ± 0.092
0.894GlyCys: 0.894 ± 0.025
4.963GlyAsp: 4.963 ± 0.056
5.003GlyGlu: 5.003 ± 0.056
2.867GlyPhe: 2.867 ± 0.044
8.478GlyGly: 8.478 ± 0.091
2.508GlyHis: 2.508 ± 0.035
3.419GlyIle: 3.419 ± 0.042
2.452GlyLys: 2.452 ± 0.041
9.467GlyLeu: 9.467 ± 0.071
1.976GlyMet: 1.976 ± 0.034
1.785GlyAsn: 1.785 ± 0.036
5.148GlyPro: 5.148 ± 0.062
2.763GlyGln: 2.763 ± 0.04
7.836GlyArg: 7.836 ± 0.065
5.505GlySer: 5.505 ± 0.067
6.58GlyThr: 6.58 ± 0.074
7.515GlyVal: 7.515 ± 0.071
1.7GlyTrp: 1.7 ± 0.032
2.309GlyTyr: 2.309 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.862HisAla: 2.862 ± 0.035
0.235HisCys: 0.235 ± 0.011
1.414HisAsp: 1.414 ± 0.026
1.272HisGlu: 1.272 ± 0.025
0.662HisPhe: 0.662 ± 0.019
2.586HisGly: 2.586 ± 0.04
0.767HisHis: 0.767 ± 0.022
0.719HisIle: 0.719 ± 0.019
0.41HisLys: 0.41 ± 0.014
2.525HisLeu: 2.525 ± 0.038
0.337HisMet: 0.337 ± 0.013
0.395HisAsn: 0.395 ± 0.013
1.947HisPro: 1.947 ± 0.04
0.702HisGln: 0.702 ± 0.02
2.256HisArg: 2.256 ± 0.035
1.166HisSer: 1.166 ± 0.022
1.508HisThr: 1.508 ± 0.03
1.787HisVal: 1.787 ± 0.033
0.39HisTrp: 0.39 ± 0.013
0.495HisTyr: 0.495 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
4.695IleAla: 4.695 ± 0.058
0.306IleCys: 0.306 ± 0.011
2.189IleAsp: 2.189 ± 0.038
1.995IleGlu: 1.995 ± 0.031
0.66IlePhe: 0.66 ± 0.019
3.525IleGly: 3.525 ± 0.044
0.599IleHis: 0.599 ± 0.017
0.917IleIle: 0.917 ± 0.026
0.745IleLys: 0.745 ± 0.021
2.413IleLeu: 2.413 ± 0.031
0.448IleMet: 0.448 ± 0.015
0.697IleAsn: 0.697 ± 0.021
1.764IlePro: 1.764 ± 0.03
0.746IleGln: 0.746 ± 0.02
2.382IleArg: 2.382 ± 0.036
1.688IleSer: 1.688 ± 0.031
2.246IleThr: 2.246 ± 0.036
2.753IleVal: 2.753 ± 0.043
0.356IleTrp: 0.356 ± 0.013
0.526IleTyr: 0.526 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
2.974LysAla: 2.974 ± 0.05
0.105LysCys: 0.105 ± 0.007
1.307LysAsp: 1.307 ± 0.036
1.159LysGlu: 1.159 ± 0.032
0.467LysPhe: 0.467 ± 0.016
1.865LysGly: 1.865 ± 0.038
0.46LysHis: 0.46 ± 0.015
0.884LysIle: 0.884 ± 0.023
0.899LysLys: 0.899 ± 0.034
2.033LysLeu: 2.033 ± 0.033
0.389LysMet: 0.389 ± 0.013
0.534LysAsn: 0.534 ± 0.021
1.36LysPro: 1.36 ± 0.03
0.708LysGln: 0.708 ± 0.022
1.508LysArg: 1.508 ± 0.033
1.153LysSer: 1.153 ± 0.028
1.359LysThr: 1.359 ± 0.03
1.927LysVal: 1.927 ± 0.035
0.262LysTrp: 0.262 ± 0.01
0.443LysTyr: 0.443 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.068LeuAla: 15.068 ± 0.116
0.889LeuCys: 0.889 ± 0.023
6.56LeuAsp: 6.56 ± 0.063
4.632LeuGlu: 4.632 ± 0.055
2.584LeuPhe: 2.584 ± 0.042
9.189LeuGly: 9.189 ± 0.081
2.517LeuHis: 2.517 ± 0.036
3.329LeuIle: 3.329 ± 0.047
2.089LeuLys: 2.089 ± 0.039
11.376LeuLeu: 11.376 ± 0.122
1.627LeuMet: 1.627 ± 0.031
1.692LeuAsn: 1.692 ± 0.031
6.556LeuPro: 6.556 ± 0.069
2.389LeuGln: 2.389 ± 0.036
8.864LeuArg: 8.864 ± 0.089
5.334LeuSer: 5.334 ± 0.056
6.87LeuThr: 6.87 ± 0.066
8.794LeuVal: 8.794 ± 0.081
1.315LeuTrp: 1.315 ± 0.027
1.872LeuTyr: 1.872 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.23MetAla: 2.23 ± 0.034
0.138MetCys: 0.138 ± 0.009
0.853MetAsp: 0.853 ± 0.019
0.74MetGlu: 0.74 ± 0.021
0.445MetPhe: 0.445 ± 0.015
1.325MetGly: 1.325 ± 0.03
0.381MetHis: 0.381 ± 0.012
0.627MetIle: 0.627 ± 0.02
0.426MetLys: 0.426 ± 0.015
1.647MetLeu: 1.647 ± 0.03
0.276MetMet: 0.276 ± 0.012
0.415MetAsn: 0.415 ± 0.014
1.099MetPro: 1.099 ± 0.024
0.466MetGln: 0.466 ± 0.014
1.448MetArg: 1.448 ± 0.025
1.276MetSer: 1.276 ± 0.024
1.538MetThr: 1.538 ± 0.025
1.291MetVal: 1.291 ± 0.026
0.211MetTrp: 0.211 ± 0.009
0.33MetTyr: 0.33 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.168AsnAla: 2.168 ± 0.034
0.166AsnCys: 0.166 ± 0.008
0.97AsnAsp: 0.97 ± 0.03
0.797AsnGlu: 0.797 ± 0.019
0.469AsnPhe: 0.469 ± 0.016
1.883AsnGly: 1.883 ± 0.033
0.408AsnHis: 0.408 ± 0.013
0.628AsnIle: 0.628 ± 0.02
0.419AsnLys: 0.419 ± 0.015
1.605AsnLeu: 1.605 ± 0.027
0.292AsnMet: 0.292 ± 0.013
0.421AsnAsn: 0.421 ± 0.015
1.373AsnPro: 1.373 ± 0.031
0.53AsnGln: 0.53 ± 0.018
1.351AsnArg: 1.351 ± 0.026
0.921AsnSer: 0.921 ± 0.023
1.124AsnThr: 1.124 ± 0.023
1.389AsnVal: 1.389 ± 0.027
0.294AsnTrp: 0.294 ± 0.013
0.395AsnTyr: 0.395 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.468ProAla: 8.468 ± 0.081
0.387ProCys: 0.387 ± 0.015
4.361ProAsp: 4.361 ± 0.048
4.22ProGlu: 4.22 ± 0.051
1.49ProPhe: 1.49 ± 0.028
6.793ProGly: 6.793 ± 0.079
1.496ProHis: 1.496 ± 0.029
1.274ProIle: 1.274 ± 0.029
1.223ProLys: 1.223 ± 0.03
5.475ProLeu: 5.475 ± 0.06
0.976ProMet: 0.976 ± 0.021
0.915ProAsn: 0.915 ± 0.023
3.538ProPro: 3.538 ± 0.062
1.798ProGln: 1.798 ± 0.035
3.946ProArg: 3.946 ± 0.048
3.306ProSer: 3.306 ± 0.045
3.262ProThr: 3.262 ± 0.045
5.721ProVal: 5.721 ± 0.052
0.871ProTrp: 0.871 ± 0.019
1.428ProTyr: 1.428 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.865GlnAla: 3.865 ± 0.053
0.201GlnCys: 0.201 ± 0.01
1.451GlnAsp: 1.451 ± 0.029
1.581GlnGlu: 1.581 ± 0.031
0.678GlnPhe: 0.678 ± 0.02
2.339GlnGly: 2.339 ± 0.04
0.739GlnHis: 0.739 ± 0.02
1.098GlnIle: 1.098 ± 0.027
0.634GlnLys: 0.634 ± 0.023
3.135GlnLeu: 3.135 ± 0.038
0.489GlnMet: 0.489 ± 0.016
0.498GlnAsn: 0.498 ± 0.018
1.825GlnPro: 1.825 ± 0.038
1.318GlnGln: 1.318 ± 0.032
2.559GlnArg: 2.559 ± 0.042
1.369GlnSer: 1.369 ± 0.028
1.413GlnThr: 1.413 ± 0.029
2.531GlnVal: 2.531 ± 0.039
0.454GlnTrp: 0.454 ± 0.015
0.646GlnTyr: 0.646 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
9.904ArgAla: 9.904 ± 0.09
0.669ArgCys: 0.669 ± 0.018
4.164ArgAsp: 4.164 ± 0.045
4.789ArgGlu: 4.789 ± 0.059
2.354ArgPhe: 2.354 ± 0.033
5.924ArgGly: 5.924 ± 0.055
2.276ArgHis: 2.276 ± 0.039
3.275ArgIle: 3.275 ± 0.042
1.718ArgLys: 1.718 ± 0.032
9.185ArgLeu: 9.185 ± 0.086
1.765ArgMet: 1.765 ± 0.029
1.41ArgAsn: 1.41 ± 0.024
5.045ArgPro: 5.045 ± 0.06
2.466ArgGln: 2.466 ± 0.037
7.856ArgArg: 7.856 ± 0.099
4.241ArgSer: 4.241 ± 0.051
5.566ArgThr: 5.566 ± 0.066
5.927ArgVal: 5.927 ± 0.063
1.393ArgTrp: 1.393 ± 0.031
1.876ArgTyr: 1.876 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
6.882SerAla: 6.882 ± 0.068
0.455SerCys: 0.455 ± 0.016
2.658SerAsp: 2.658 ± 0.042
2.4SerGlu: 2.4 ± 0.038
1.511SerPhe: 1.511 ± 0.028
6.169SerGly: 6.169 ± 0.077
1.075SerHis: 1.075 ± 0.024
1.433SerIle: 1.433 ± 0.027
1.073SerLys: 1.073 ± 0.023
4.911SerLeu: 4.911 ± 0.051
1.056SerMet: 1.056 ± 0.023
0.862SerAsn: 0.862 ± 0.023
3.209SerPro: 3.209 ± 0.042
1.314SerGln: 1.314 ± 0.024
3.769SerArg: 3.769 ± 0.048
2.97SerSer: 2.97 ± 0.053
3.234SerThr: 3.234 ± 0.044
4.294SerVal: 4.294 ± 0.042
0.879SerTrp: 0.879 ± 0.02
1.262SerTyr: 1.262 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
9.014ThrAla: 9.014 ± 0.079
0.484ThrCys: 0.484 ± 0.015
3.677ThrAsp: 3.677 ± 0.048
3.339ThrGlu: 3.339 ± 0.039
1.601ThrPhe: 1.601 ± 0.027
6.936ThrGly: 6.936 ± 0.069
1.276ThrHis: 1.276 ± 0.025
1.695ThrIle: 1.695 ± 0.035
1.176ThrLys: 1.176 ± 0.031
5.69ThrLeu: 5.69 ± 0.068
0.952ThrMet: 0.952 ± 0.023
0.988ThrAsn: 0.988 ± 0.021
4.163ThrPro: 4.163 ± 0.047
1.425ThrGln: 1.425 ± 0.03
4.091ThrArg: 4.091 ± 0.046
3.227ThrSer: 3.227 ± 0.045
3.961ThrThr: 3.961 ± 0.07
6.18ThrVal: 6.18 ± 0.055
0.921ThrTrp: 0.921 ± 0.023
1.379ThrTyr: 1.379 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
10.52ValAla: 10.52 ± 0.086
0.825ValCys: 0.825 ± 0.022
4.887ValAsp: 4.887 ± 0.057
4.623ValGlu: 4.623 ± 0.052
2.426ValPhe: 2.426 ± 0.036
6.449ValGly: 6.449 ± 0.061
2.169ValHis: 2.169 ± 0.033
2.965ValIle: 2.965 ± 0.044
1.762ValLys: 1.762 ± 0.038
9.623ValLeu: 9.623 ± 0.084
1.412ValMet: 1.412 ± 0.028
1.639ValAsn: 1.639 ± 0.031
5.406ValPro: 5.406 ± 0.063
2.258ValGln: 2.258 ± 0.035
7.359ValArg: 7.359 ± 0.074
4.407ValSer: 4.407 ± 0.052
5.64ValThr: 5.64 ± 0.056
7.916ValVal: 7.916 ± 0.086
1.143ValTrp: 1.143 ± 0.025
1.665ValTyr: 1.665 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.628TrpAla: 1.628 ± 0.031
0.168TrpCys: 0.168 ± 0.009
0.787TrpAsp: 0.787 ± 0.022
0.721TrpGlu: 0.721 ± 0.02
0.502TrpPhe: 0.502 ± 0.018
1.034TrpGly: 1.034 ± 0.021
0.394TrpHis: 0.394 ± 0.012
0.546TrpIle: 0.546 ± 0.016
0.366TrpLys: 0.366 ± 0.016
1.779TrpLeu: 1.779 ± 0.03
0.279TrpMet: 0.279 ± 0.011
0.394TrpAsn: 0.394 ± 0.016
0.766TrpPro: 0.766 ± 0.02
0.631TrpGln: 0.631 ± 0.016
1.348TrpArg: 1.348 ± 0.029
0.889TrpSer: 0.889 ± 0.021
1.008TrpThr: 1.008 ± 0.023
0.954TrpVal: 0.954 ± 0.025
0.351TrpTrp: 0.351 ± 0.015
0.358TrpTyr: 0.358 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.774TyrAla: 2.774 ± 0.032
0.18TyrCys: 0.18 ± 0.009
1.617TyrAsp: 1.617 ± 0.036
1.25TyrGlu: 1.25 ± 0.027
0.645TyrPhe: 0.645 ± 0.019
2.375TyrGly: 2.375 ± 0.041
0.412TyrHis: 0.412 ± 0.015
0.477TyrIle: 0.477 ± 0.017
0.395TyrLys: 0.395 ± 0.014
2.109TyrLeu: 2.109 ± 0.033
0.262TyrMet: 0.262 ± 0.012
0.405TyrAsn: 0.405 ± 0.015
1.087TyrPro: 1.087 ± 0.023
0.662TyrGln: 0.662 ± 0.019
1.854TyrArg: 1.854 ± 0.034
0.949TyrSer: 0.949 ± 0.023
1.224TyrThr: 1.224 ± 0.024
1.626TyrVal: 1.626 ± 0.03
0.356TyrTrp: 0.356 ± 0.014
0.446TyrTyr: 0.446 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6158 proteins (2045619 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski