Desmodium leaf distortion virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q06HP8|Q06HP8_9GEMI Transcriptional activator protein OS=Desmodium leaf distortion virus OX=361731 GN=AC2 PE=3 SV=1
MM1 pKa = 7.61PLKK4 pKa = 10.58PKK6 pKa = 9.04TFRR9 pKa = 11.84LAARR13 pKa = 11.84NIFLTYY19 pKa = 8.33PQCDD23 pKa = 3.41IPKK26 pKa = 10.5DD27 pKa = 3.43EE28 pKa = 4.99ALQMLQNLSWSVVKK42 pKa = 9.76PVYY45 pKa = 10.13IRR47 pKa = 11.84VARR50 pKa = 11.84EE51 pKa = 3.47EE52 pKa = 4.18HH53 pKa = 6.79SDD55 pKa = 3.74GFPHH59 pKa = 6.91LHH61 pKa = 6.99CLLQLSGKK69 pKa = 10.19SNIKK73 pKa = 9.85DD74 pKa = 3.18ARR76 pKa = 11.84FFDD79 pKa = 3.84LTHH82 pKa = 6.84PRR84 pKa = 11.84RR85 pKa = 11.84SANFHH90 pKa = 6.88PNVQAAKK97 pKa = 10.4DD98 pKa = 3.79SNAVRR103 pKa = 11.84NYY105 pKa = 7.41ITKK108 pKa = 10.47EE109 pKa = 3.36GDD111 pKa = 3.42YY112 pKa = 10.99CEE114 pKa = 4.39SGQYY118 pKa = 9.66KK119 pKa = 9.81VPGGTKK125 pKa = 10.13ANKK128 pKa = 10.13DD129 pKa = 3.82DD130 pKa = 3.94VFHH133 pKa = 6.74NAVNAPGVAEE143 pKa = 4.23ALDD146 pKa = 4.3IIRR149 pKa = 11.84AGDD152 pKa = 3.56PRR154 pKa = 11.84TFIVSYY160 pKa = 10.93HH161 pKa = 5.96NIKK164 pKa = 10.95ANIEE168 pKa = 3.97RR169 pKa = 11.84LFAKK173 pKa = 9.99PPEE176 pKa = 4.58PWTPPYY182 pKa = 10.26RR183 pKa = 11.84LSSFTNVPDD192 pKa = 5.1EE193 pKa = 4.14MQEE196 pKa = 3.79WADD199 pKa = 3.84DD200 pKa = 3.8YY201 pKa = 11.36FGRR204 pKa = 11.84SSAARR209 pKa = 11.84PEE211 pKa = 4.06RR212 pKa = 11.84PISIIVEE219 pKa = 4.19GEE221 pKa = 3.93SRR223 pKa = 11.84TGKK226 pKa = 8.58TMWARR231 pKa = 11.84SLGPHH236 pKa = 6.57NYY238 pKa = 10.17LSGHH242 pKa = 6.8LDD244 pKa = 3.41FNNRR248 pKa = 11.84VYY250 pKa = 11.26SNEE253 pKa = 3.59VEE255 pKa = 4.32YY256 pKa = 11.37NVIDD260 pKa = 4.42DD261 pKa = 4.55VAPQYY266 pKa = 11.38LKK268 pKa = 10.74LKK270 pKa = 9.13HH271 pKa = 5.51WKK273 pKa = 9.67EE274 pKa = 3.97LIGAQKK280 pKa = 10.4NWQSNCKK287 pKa = 9.26YY288 pKa = 10.21GKK290 pKa = 9.18PVQIKK295 pKa = 10.19GGIPSIVLCNPGEE308 pKa = 4.16GASYY312 pKa = 11.36KK313 pKa = 10.89DD314 pKa = 3.85FLDD317 pKa = 3.69KK318 pKa = 11.49AEE320 pKa = 4.19NASLRR325 pKa = 11.84NWTLKK330 pKa = 10.4NAIFITLTSALYY342 pKa = 10.51QEE344 pKa = 5.71GPQASQEE351 pKa = 4.22EE352 pKa = 5.07GGQTTPDD359 pKa = 3.09

Molecular weight: 40.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q06HQ0|Q06HQ0_9GEMI Capsid protein OS=Desmodium leaf distortion virus OX=361731 GN=AV1 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.61RR4 pKa = 11.84DD5 pKa = 3.78APWRR9 pKa = 11.84SMAGTSKK16 pKa = 10.54VSRR19 pKa = 11.84AANYY23 pKa = 10.19SPRR26 pKa = 11.84AGNGPKK32 pKa = 10.3LNRR35 pKa = 11.84ASEE38 pKa = 4.24WVNRR42 pKa = 11.84PMYY45 pKa = 9.79RR46 pKa = 11.84KK47 pKa = 8.95PRR49 pKa = 11.84IYY51 pKa = 9.54RR52 pKa = 11.84TLRR55 pKa = 11.84SPDD58 pKa = 3.26VPRR61 pKa = 11.84GCEE64 pKa = 4.34GPCKK68 pKa = 9.32VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.13VGKK83 pKa = 10.55VMCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.24ILGKK114 pKa = 9.73VWMDD118 pKa = 3.29EE119 pKa = 4.01NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.81NDD166 pKa = 2.71LRR168 pKa = 11.84DD169 pKa = 3.53RR170 pKa = 11.84YY171 pKa = 10.4RR172 pKa = 11.84VMHH175 pKa = 6.65KK176 pKa = 9.86FYY178 pKa = 11.28AKK180 pKa = 9.12VTGGQYY186 pKa = 11.07ASNEE190 pKa = 3.69QAIVKK195 pKa = 8.86RR196 pKa = 11.84FWRR199 pKa = 11.84INTHH203 pKa = 4.31VVYY206 pKa = 10.68NHH208 pKa = 5.77QEE210 pKa = 3.45AAKK213 pKa = 10.4YY214 pKa = 9.25EE215 pKa = 4.13NHH217 pKa = 6.46TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.09ASNLVYY236 pKa = 9.81ATLKK240 pKa = 9.88IRR242 pKa = 11.84IYY244 pKa = 10.6FYY246 pKa = 11.26DD247 pKa = 4.02SIMNN251 pKa = 4.24

Molecular weight: 29.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1421

129

359

236.8

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.982 ± 0.786

1.689 ± 0.246

5.278 ± 0.474

4.785 ± 0.411

3.941 ± 0.227

5.348 ± 0.385

3.871 ± 0.522

5.63 ± 0.432

5.911 ± 0.438

6.967 ± 0.606

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.535

5.489 ± 0.734

5.559 ± 0.578

3.73 ± 0.599

7.671 ± 0.759

8.234 ± 1.315

5.067 ± 0.456

6.474 ± 0.545

1.619 ± 0.23

4.504 ± 0.589
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski