Odocoileus adenovirus 1
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q98XR5|Q98XR5_9ADEN Fiber OS=Odocoileus adenovirus 1 OX=78522 PE=3 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.04 RR3 pKa = 11.84 ARR5 pKa = 11.84 WDD7 pKa = 3.19 PVYY10 pKa = 10.46 PFSEE14 pKa = 4.7 DD15 pKa = 3.13 KK16 pKa = 10.99 LIPLPPFIQAGTGLEE31 pKa = 4.17 SEE33 pKa = 4.66 GMILSLNFTDD43 pKa = 6.32 PITINPTGSLTIKK56 pKa = 9.76 IGPGIEE62 pKa = 3.95 INEE65 pKa = 4.21 NGQLTATAQALIAEE79 pKa = 4.64 SPLKK83 pKa = 10.42 KK84 pKa = 10.07 EE85 pKa = 3.95 NYY87 pKa = 8.67 ILKK90 pKa = 9.66 IQTNNTLVTEE100 pKa = 4.39 DD101 pKa = 4.73 DD102 pKa = 3.75 KK103 pKa = 11.63 MGIPDD108 pKa = 4.43 PLEE111 pKa = 3.99 PLNIEE116 pKa = 4.21 SSGQLSLNFDD126 pKa = 3.27 KK127 pKa = 11.2 GFQLFEE133 pKa = 4.65 GKK135 pKa = 10.53 LSLYY139 pKa = 10.34 LDD141 pKa = 3.4 NSLFIKK147 pKa = 10.61 NEE149 pKa = 3.8 KK150 pKa = 10.37 LSLKK154 pKa = 10.41 EE155 pKa = 4.12 PIEE158 pKa = 3.8 PLIINSQGQLNLNYY172 pKa = 10.47 DD173 pKa = 3.71 NSLVLQDD180 pKa = 4.6 HH181 pKa = 6.62 KK182 pKa = 11.44 LSVPEE187 pKa = 4.05 PLEE190 pKa = 4.11 PLDD193 pKa = 5.31 LEE195 pKa = 4.66 STGQLALSINKK206 pKa = 8.81 GFQISDD212 pKa = 3.03 GRR214 pKa = 11.84 LTLDD218 pKa = 3.43 IANCFQYY225 pKa = 11.13 NSNKK229 pKa = 8.7 LTLTNPTTPIVIKK242 pKa = 8.73 PTGEE246 pKa = 3.29 ISLNIGEE253 pKa = 4.82 GLTVDD258 pKa = 4.54 EE259 pKa = 6.02 GNLTLNTNDD268 pKa = 3.51 TLQIEE273 pKa = 4.79 DD274 pKa = 3.92 GQITLTDD281 pKa = 3.42 PMEE284 pKa = 4.47 PLNLEE289 pKa = 3.9 MGQLVLNVDD298 pKa = 3.55 NVFKK302 pKa = 11.37 VEE304 pKa = 3.84 NGKK307 pKa = 10.37 LGLNVNSPMSINNNNLNINLGQGLEE332 pKa = 4.09 IKK334 pKa = 10.47 QGALTPKK341 pKa = 9.84 ISSPLIFNSSGEE353 pKa = 4.26 ITTSTTQGSQIIPFDD368 pKa = 3.77 AYY370 pKa = 10.4 EE371 pKa = 4.73 LVLMWQKK378 pKa = 10.92 FLTTRR383 pKa = 11.84 HH384 pKa = 6.1 AIYY387 pKa = 10.58 VLHH390 pKa = 6.36 CTSFIPDD397 pKa = 3.63 TSVDD401 pKa = 4.02 TITFTPNDD409 pKa = 3.71 GLKK412 pKa = 10.76 AFLSTDD418 pKa = 3.28 SPLFTAAFQQVGNEE432 pKa = 3.92 VKK434 pKa = 10.07 TIGVKK439 pKa = 10.65 FSKK442 pKa = 10.57 ISDD445 pKa = 3.63 TEE447 pKa = 4.06 VQIKK451 pKa = 8.94 YY452 pKa = 10.38 SQMLTTQVILSSWTATTALFVV473 pKa = 3.62
Molecular weight: 51.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.564
IPC2_protein 4.457
IPC_protein 4.406
Toseland 4.24
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.215
Rodwell 4.24
Grimsley 4.139
Solomon 4.342
Lehninger 4.304
Nozaki 4.457
DTASelect 4.622
Thurlkill 4.24
EMBOSS 4.24
Sillero 4.507
Patrickios 3.999
IPC_peptide 4.355
IPC2_peptide 4.495
IPC2.peptide.svr19 4.472
Protein with the highest isoelectric point:
>tr|A0A223PYU3|A0A223PYU3_9ADEN PVI OS=Odocoileus adenovirus 1 OX=78522 PE=4 SV=1
MM1 pKa = 7.6 EE2 pKa = 5.57 SMPHH6 pKa = 7.36 LYY8 pKa = 9.93 TLMGGATRR16 pKa = 11.84 KK17 pKa = 8.93 KK18 pKa = 9.79 RR19 pKa = 11.84 KK20 pKa = 9.4 RR21 pKa = 11.84 RR22 pKa = 11.84 TITRR26 pKa = 11.84 KK27 pKa = 9.69 RR28 pKa = 11.84 NPIKK32 pKa = 10.63 SSNRR36 pKa = 11.84 KK37 pKa = 5.99 ITRR40 pKa = 11.84 GTRR43 pKa = 11.84 RR44 pKa = 11.84 LKK46 pKa = 10.29 HH47 pKa = 4.58 VRR49 pKa = 11.84 IPIVSQYY56 pKa = 11.54 LNIHH60 pKa = 6.85 PPQALNKK67 pKa = 8.33 QHH69 pKa = 6.37 QPLFYY74 pKa = 10.59 HH75 pKa = 7.05 HH76 pKa = 7.16 FNQHH80 pKa = 5.29 LHH82 pKa = 5.89 HH83 pKa = 6.59 QPNQPLVGTSYY94 pKa = 11.0 PMPSTSIFIHH104 pKa = 6.88 PPTINNSFTKK114 pKa = 10.41 NVSLKK119 pKa = 10.07 RR120 pKa = 11.84 DD121 pKa = 3.66 HH122 pKa = 6.94 QPDD125 pKa = 4.46 EE126 pKa = 4.01 IQVFHH131 pKa = 7.61 DD132 pKa = 4.02 AQDD135 pKa = 3.14 HH136 pKa = 5.61 FEE138 pKa = 4.14 IKK140 pKa = 9.77 RR141 pKa = 11.84 TDD143 pKa = 3.38 GEE145 pKa = 4.07 NDD147 pKa = 2.92 SDD149 pKa = 4.37 INAKK153 pKa = 10.15 KK154 pKa = 9.98 KK155 pKa = 9.58 WSLEE159 pKa = 3.96 DD160 pKa = 3.38 VLSFLQKK167 pKa = 10.3 VPHH170 pKa = 5.56 QLRR173 pKa = 11.84 KK174 pKa = 9.87 IILTSLFGATIGLLFDD190 pKa = 5.2 LLLGGPWGLTTRR202 pKa = 11.84 LLRR205 pKa = 11.84 LIISLVPGGNIFLTALDD222 pKa = 3.8 GLGYY226 pKa = 11.03 LLGKK230 pKa = 9.3 SANPFNIAYY239 pKa = 10.14 DD240 pKa = 3.59 PDD242 pKa = 3.72 FQNFGNIIQSKK253 pKa = 8.87 MNDD256 pKa = 3.11 RR257 pKa = 11.84 LAEE260 pKa = 4.3 DD261 pKa = 3.53 VAKK264 pKa = 10.51 AAEE267 pKa = 4.03 EE268 pKa = 4.0 QIGNGFMRR276 pKa = 11.84 TLASLLSAAASAGTHH291 pKa = 6.24 LKK293 pKa = 10.32 LALPAIPIAAIRR305 pKa = 11.84 PFQRR309 pKa = 4.05
Molecular weight: 34.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.56
IPC_protein 9.736
Toseland 10.54
ProMoST 10.145
Dawson 10.628
Bjellqvist 10.248
Wikipedia 10.774
Rodwell 11.111
Grimsley 10.672
Solomon 10.672
Lehninger 10.657
Nozaki 10.496
DTASelect 10.248
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.54
Patrickios 10.818
IPC_peptide 10.687
IPC2_peptide 8.712
IPC2.peptide.svr19 8.752
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27
0
27
9142
54
1075
338.6
38.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.857 ± 0.321
2.253 ± 0.395
4.857 ± 0.252
5.819 ± 0.326
5.568 ± 0.237
5.075 ± 0.337
2.385 ± 0.303
6.858 ± 0.416
6.629 ± 0.624
9.473 ± 0.516
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.024 ± 0.161
6.596 ± 0.338
5.021 ± 0.315
4.507 ± 0.263
4.419 ± 0.55
7.208 ± 0.301
6.257 ± 0.309
4.791 ± 0.367
1.488 ± 0.157
3.894 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here