Ralstonia phage RsoP1EGY

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Gyeongsanvirus; Ralstonia virus RsoP1EGY

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R2ZGB7|A0A2R2ZGB7_9CAUD Uncharacterized protein OS=Ralstonia phage RsoP1EGY OX=2070026 GN=RSEGYP2_4 PE=4 SV=1
MM1 pKa = 6.07QTKK4 pKa = 7.39EE5 pKa = 3.76QRR7 pKa = 11.84IEE9 pKa = 4.35LIAAMFGEE17 pKa = 4.54QEE19 pKa = 3.85TGLIGKK25 pKa = 7.44QLRR28 pKa = 11.84VLDD31 pKa = 4.28NSQSGAFYY39 pKa = 10.92NVGDD43 pKa = 3.8VGTVVLVDD51 pKa = 4.18DD52 pKa = 5.75DD53 pKa = 4.67GEE55 pKa = 4.08IWVDD59 pKa = 4.22FGPDD63 pKa = 3.23GFKK66 pKa = 11.09GDD68 pKa = 3.57GTAYY72 pKa = 9.31PVWAAGSLGADD83 pKa = 2.87DD84 pKa = 6.35HH85 pKa = 7.01EE86 pKa = 4.59FLEE89 pKa = 4.54NN90 pKa = 3.41

Molecular weight: 9.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R2ZGF7|A0A2R2ZGF7_9CAUD Uncharacterized protein OS=Ralstonia phage RsoP1EGY OX=2070026 GN=RSEGYP2_22 PE=4 SV=1
MM1 pKa = 7.19WWSFTTSKK9 pKa = 10.01EE10 pKa = 4.36RR11 pKa = 11.84NWNTSEE17 pKa = 3.6RR18 pKa = 11.84RR19 pKa = 11.84SRR21 pKa = 11.84EE22 pKa = 3.79RR23 pKa = 11.84YY24 pKa = 9.26RR25 pKa = 11.84DD26 pKa = 3.56RR27 pKa = 11.84QDD29 pKa = 2.98EE30 pKa = 4.2MGAGFGKK37 pKa = 10.51ALRR40 pKa = 11.84RR41 pKa = 11.84TGDD44 pKa = 2.87MGTCGTQKK52 pKa = 10.1PRR54 pKa = 11.84SLSQHH59 pKa = 6.12IDD61 pKa = 3.33CPTNSTRR68 pKa = 11.84DD69 pKa = 3.19KK70 pKa = 10.94SRR72 pKa = 11.84RR73 pKa = 11.84GG74 pKa = 3.28

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12064

52

1573

241.3

26.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.823 ± 0.412

0.986 ± 0.147

5.769 ± 0.205

6.192 ± 0.462

3.796 ± 0.186

8.04 ± 0.443

1.865 ± 0.202

4.418 ± 0.203

5.985 ± 0.37

8.256 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.204

4.087 ± 0.317

4.882 ± 0.233

3.904 ± 0.23

6.109 ± 0.327

5.943 ± 0.351

6.101 ± 0.377

7.004 ± 0.417

1.592 ± 0.144

2.818 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski