Amino acid dipepetide frequency for Ralstonia phage RsoP1EGY

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.854AlaAla: 11.854 ± 1.27
0.332AlaCys: 0.332 ± 0.142
5.554AlaAsp: 5.554 ± 0.689
6.466AlaGlu: 6.466 ± 0.885
3.15AlaPhe: 3.15 ± 0.361
7.958AlaGly: 7.958 ± 0.627
1.243AlaHis: 1.243 ± 0.288
4.062AlaIle: 4.062 ± 0.596
5.886AlaLys: 5.886 ± 0.776
8.787AlaLeu: 8.787 ± 0.767
2.321AlaMet: 2.321 ± 0.482
4.891AlaAsn: 4.891 ± 0.71
3.233AlaPro: 3.233 ± 0.683
4.145AlaGln: 4.145 ± 0.634
5.554AlaArg: 5.554 ± 0.645
6.217AlaSer: 6.217 ± 0.863
5.388AlaThr: 5.388 ± 0.756
6.632AlaVal: 6.632 ± 0.867
1.658AlaTrp: 1.658 ± 0.388
2.901AlaTyr: 2.901 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
0.995CysAla: 0.995 ± 0.296
0.166CysCys: 0.166 ± 0.127
0.332CysAsp: 0.332 ± 0.141
0.58CysGlu: 0.58 ± 0.249
0.58CysPhe: 0.58 ± 0.236
0.746CysGly: 0.746 ± 0.275
0.414CysHis: 0.414 ± 0.247
0.58CysIle: 0.58 ± 0.212
1.078CysLys: 1.078 ± 0.474
0.497CysLeu: 0.497 ± 0.212
0.083CysMet: 0.083 ± 0.097
0.414CysAsn: 0.414 ± 0.233
0.829CysPro: 0.829 ± 0.297
0.414CysGln: 0.414 ± 0.207
0.58CysArg: 0.58 ± 0.239
0.58CysSer: 0.58 ± 0.21
0.166CysThr: 0.166 ± 0.098
0.58CysVal: 0.58 ± 0.232
0.249CysTrp: 0.249 ± 0.154
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.974AspAla: 4.974 ± 0.687
0.414AspCys: 0.414 ± 0.175
3.896AspAsp: 3.896 ± 0.742
3.399AspGlu: 3.399 ± 0.566
2.901AspPhe: 2.901 ± 0.538
5.14AspGly: 5.14 ± 0.804
1.243AspHis: 1.243 ± 0.339
3.565AspIle: 3.565 ± 0.558
3.399AspLys: 3.399 ± 0.591
5.14AspLeu: 5.14 ± 0.627
1.575AspMet: 1.575 ± 0.319
3.15AspAsn: 3.15 ± 0.415
2.404AspPro: 2.404 ± 0.43
1.078AspGln: 1.078 ± 0.369
3.316AspArg: 3.316 ± 0.345
2.155AspSer: 2.155 ± 0.391
3.565AspThr: 3.565 ± 0.578
3.316AspVal: 3.316 ± 0.557
0.746AspTrp: 0.746 ± 0.187
2.321AspTyr: 2.321 ± 0.414
0.0AspXaa: 0.0 ± 0.0
Glu
6.134GluAla: 6.134 ± 0.705
0.497GluCys: 0.497 ± 0.192
2.819GluAsp: 2.819 ± 0.706
5.305GluGlu: 5.305 ± 0.911
3.15GluPhe: 3.15 ± 0.542
4.559GluGly: 4.559 ± 0.976
1.078GluHis: 1.078 ± 0.259
1.824GluIle: 1.824 ± 0.371
4.476GluLys: 4.476 ± 0.714
5.72GluLeu: 5.72 ± 0.608
1.658GluMet: 1.658 ± 0.427
3.067GluAsn: 3.067 ± 0.353
2.653GluPro: 2.653 ± 0.494
2.072GluGln: 2.072 ± 0.411
3.896GluArg: 3.896 ± 0.624
3.15GluSer: 3.15 ± 0.524
2.984GluThr: 2.984 ± 0.699
3.565GluVal: 3.565 ± 0.55
1.492GluTrp: 1.492 ± 0.431
1.824GluTyr: 1.824 ± 0.358
0.0GluXaa: 0.0 ± 0.0
Phe
2.901PheAla: 2.901 ± 0.476
0.166PheCys: 0.166 ± 0.114
2.984PheAsp: 2.984 ± 0.481
2.072PheGlu: 2.072 ± 0.472
0.829PhePhe: 0.829 ± 0.262
3.316PheGly: 3.316 ± 0.643
0.414PheHis: 0.414 ± 0.159
1.658PheIle: 1.658 ± 0.356
2.072PheLys: 2.072 ± 0.361
2.404PheLeu: 2.404 ± 0.43
1.078PheMet: 1.078 ± 0.323
1.492PheAsn: 1.492 ± 0.302
1.741PhePro: 1.741 ± 0.348
1.492PheGln: 1.492 ± 0.367
3.813PheArg: 3.813 ± 0.453
2.984PheSer: 2.984 ± 0.528
2.487PheThr: 2.487 ± 0.457
2.653PheVal: 2.653 ± 0.34
0.414PheTrp: 0.414 ± 0.163
0.995PheTyr: 0.995 ± 0.308
0.0PheXaa: 0.0 ± 0.0
Gly
6.632GlyAla: 6.632 ± 0.693
0.663GlyCys: 0.663 ± 0.241
5.305GlyAsp: 5.305 ± 0.689
4.311GlyGlu: 4.311 ± 0.713
4.062GlyPhe: 4.062 ± 0.494
5.886GlyGly: 5.886 ± 0.981
1.741GlyHis: 1.741 ± 0.465
4.145GlyIle: 4.145 ± 0.586
5.14GlyLys: 5.14 ± 0.67
7.295GlyLeu: 7.295 ± 0.709
1.741GlyMet: 1.741 ± 0.358
3.648GlyAsn: 3.648 ± 0.571
1.658GlyPro: 1.658 ± 0.403
2.653GlyGln: 2.653 ± 0.469
4.311GlyArg: 4.311 ± 0.659
5.471GlySer: 5.471 ± 0.732
5.803GlyThr: 5.803 ± 0.875
5.72GlyVal: 5.72 ± 0.676
1.409GlyTrp: 1.409 ± 0.401
2.819GlyTyr: 2.819 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.243HisAla: 1.243 ± 0.309
0.414HisCys: 0.414 ± 0.208
0.912HisAsp: 0.912 ± 0.244
0.829HisGlu: 0.829 ± 0.242
0.995HisPhe: 0.995 ± 0.242
1.824HisGly: 1.824 ± 0.405
0.332HisHis: 0.332 ± 0.244
0.912HisIle: 0.912 ± 0.259
0.58HisLys: 0.58 ± 0.224
2.487HisLeu: 2.487 ± 0.534
0.414HisMet: 0.414 ± 0.155
0.746HisAsn: 0.746 ± 0.246
0.746HisPro: 0.746 ± 0.217
0.58HisGln: 0.58 ± 0.201
1.326HisArg: 1.326 ± 0.356
1.243HisSer: 1.243 ± 0.318
1.078HisThr: 1.078 ± 0.288
1.326HisVal: 1.326 ± 0.284
0.414HisTrp: 0.414 ± 0.147
0.249HisTyr: 0.249 ± 0.122
0.0HisXaa: 0.0 ± 0.0
Ile
5.057IleAla: 5.057 ± 0.547
0.663IleCys: 0.663 ± 0.299
2.984IleAsp: 2.984 ± 0.516
2.901IleGlu: 2.901 ± 0.429
0.332IlePhe: 0.332 ± 0.163
3.813IleGly: 3.813 ± 0.499
0.829IleHis: 0.829 ± 0.282
2.487IleIle: 2.487 ± 0.523
2.487IleLys: 2.487 ± 0.459
3.648IleLeu: 3.648 ± 0.576
0.332IleMet: 0.332 ± 0.174
1.99IleAsn: 1.99 ± 0.382
2.653IlePro: 2.653 ± 0.587
2.072IleGln: 2.072 ± 0.424
2.072IleArg: 2.072 ± 0.423
3.15IleSer: 3.15 ± 0.463
2.653IleThr: 2.653 ± 0.621
2.404IleVal: 2.404 ± 0.442
0.829IleTrp: 0.829 ± 0.203
0.829IleTyr: 0.829 ± 0.209
0.0IleXaa: 0.0 ± 0.0
Lys
7.129LysAla: 7.129 ± 0.863
0.497LysCys: 0.497 ± 0.156
3.896LysAsp: 3.896 ± 0.488
2.653LysGlu: 2.653 ± 0.426
1.99LysPhe: 1.99 ± 0.342
4.974LysGly: 4.974 ± 0.853
1.492LysHis: 1.492 ± 0.341
2.487LysIle: 2.487 ± 0.485
3.648LysLys: 3.648 ± 0.701
5.223LysLeu: 5.223 ± 0.777
0.829LysMet: 0.829 ± 0.301
1.741LysAsn: 1.741 ± 0.353
2.736LysPro: 2.736 ± 0.523
2.321LysGln: 2.321 ± 0.459
3.979LysArg: 3.979 ± 0.455
3.316LysSer: 3.316 ± 0.573
3.233LysThr: 3.233 ± 0.473
4.725LysVal: 4.725 ± 0.581
1.409LysTrp: 1.409 ± 0.343
1.575LysTyr: 1.575 ± 0.386
0.0LysXaa: 0.0 ± 0.0
Leu
7.544LeuAla: 7.544 ± 1.03
0.497LeuCys: 0.497 ± 0.227
4.062LeuAsp: 4.062 ± 0.48
5.637LeuGlu: 5.637 ± 0.827
2.404LeuPhe: 2.404 ± 0.436
7.046LeuGly: 7.046 ± 0.742
1.824LeuHis: 1.824 ± 0.444
3.067LeuIle: 3.067 ± 0.485
5.886LeuLys: 5.886 ± 0.718
5.886LeuLeu: 5.886 ± 0.653
2.487LeuMet: 2.487 ± 0.416
3.316LeuAsn: 3.316 ± 0.522
4.891LeuPro: 4.891 ± 0.805
3.067LeuGln: 3.067 ± 0.461
5.886LeuArg: 5.886 ± 0.908
5.388LeuSer: 5.388 ± 0.528
4.311LeuThr: 4.311 ± 0.514
5.554LeuVal: 5.554 ± 0.612
0.829LeuTrp: 0.829 ± 0.3
2.984LeuTyr: 2.984 ± 0.613
0.0LeuXaa: 0.0 ± 0.0
Met
2.404MetAla: 2.404 ± 0.355
0.166MetCys: 0.166 ± 0.114
1.078MetAsp: 1.078 ± 0.365
1.078MetGlu: 1.078 ± 0.254
1.161MetPhe: 1.161 ± 0.32
1.741MetGly: 1.741 ± 0.589
0.414MetHis: 0.414 ± 0.175
0.746MetIle: 0.746 ± 0.321
1.658MetLys: 1.658 ± 0.44
1.658MetLeu: 1.658 ± 0.326
0.663MetMet: 0.663 ± 0.283
0.58MetAsn: 0.58 ± 0.213
1.078MetPro: 1.078 ± 0.288
1.243MetGln: 1.243 ± 0.344
1.575MetArg: 1.575 ± 0.409
2.238MetSer: 2.238 ± 0.481
1.741MetThr: 1.741 ± 0.307
1.741MetVal: 1.741 ± 0.49
0.497MetTrp: 0.497 ± 0.203
0.829MetTyr: 0.829 ± 0.259
0.0MetXaa: 0.0 ± 0.0
Asn
3.316AsnAla: 3.316 ± 0.588
0.497AsnCys: 0.497 ± 0.231
1.741AsnAsp: 1.741 ± 0.364
2.653AsnGlu: 2.653 ± 0.362
2.155AsnPhe: 2.155 ± 0.495
3.813AsnGly: 3.813 ± 0.63
0.497AsnHis: 0.497 ± 0.223
1.326AsnIle: 1.326 ± 0.317
2.072AsnLys: 2.072 ± 0.344
3.979AsnLeu: 3.979 ± 0.584
0.746AsnMet: 0.746 ± 0.294
1.658AsnAsn: 1.658 ± 0.42
2.487AsnPro: 2.487 ± 0.389
1.99AsnGln: 1.99 ± 0.507
2.072AsnArg: 2.072 ± 0.47
3.067AsnSer: 3.067 ± 0.359
2.404AsnThr: 2.404 ± 0.673
2.984AsnVal: 2.984 ± 0.484
0.663AsnTrp: 0.663 ± 0.22
0.746AsnTyr: 0.746 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
5.305ProAla: 5.305 ± 0.686
0.414ProCys: 0.414 ± 0.2
2.901ProAsp: 2.901 ± 0.555
3.316ProGlu: 3.316 ± 0.618
1.824ProPhe: 1.824 ± 0.399
3.316ProGly: 3.316 ± 0.551
0.746ProHis: 0.746 ± 0.211
0.995ProIle: 0.995 ± 0.279
2.819ProLys: 2.819 ± 0.396
3.233ProLeu: 3.233 ± 0.549
1.409ProMet: 1.409 ± 0.31
1.326ProAsn: 1.326 ± 0.213
3.813ProPro: 3.813 ± 1.277
1.824ProGln: 1.824 ± 0.445
2.404ProArg: 2.404 ± 0.433
2.736ProSer: 2.736 ± 0.521
3.482ProThr: 3.482 ± 0.664
4.476ProVal: 4.476 ± 0.574
0.995ProTrp: 0.995 ± 0.326
1.492ProTyr: 1.492 ± 0.297
0.0ProXaa: 0.0 ± 0.0
Gln
4.311GlnAla: 4.311 ± 0.83
0.249GlnCys: 0.249 ± 0.153
1.824GlnAsp: 1.824 ± 0.357
2.901GlnGlu: 2.901 ± 0.493
1.99GlnPhe: 1.99 ± 0.476
2.57GlnGly: 2.57 ± 0.44
1.161GlnHis: 1.161 ± 0.363
1.741GlnIle: 1.741 ± 0.348
2.736GlnLys: 2.736 ± 0.517
2.901GlnLeu: 2.901 ± 0.465
1.078GlnMet: 1.078 ± 0.346
1.326GlnAsn: 1.326 ± 0.283
1.326GlnPro: 1.326 ± 0.416
2.238GlnGln: 2.238 ± 0.445
2.238GlnArg: 2.238 ± 0.461
1.907GlnSer: 1.907 ± 0.474
1.824GlnThr: 1.824 ± 0.488
2.736GlnVal: 2.736 ± 0.408
0.746GlnTrp: 0.746 ± 0.2
1.161GlnTyr: 1.161 ± 0.275
0.0GlnXaa: 0.0 ± 0.0
Arg
5.969ArgAla: 5.969 ± 0.776
0.829ArgCys: 0.829 ± 0.361
4.311ArgAsp: 4.311 ± 0.598
3.648ArgGlu: 3.648 ± 0.527
2.238ArgPhe: 2.238 ± 0.408
5.057ArgGly: 5.057 ± 0.6
0.995ArgHis: 0.995 ± 0.219
3.482ArgIle: 3.482 ± 0.455
2.984ArgLys: 2.984 ± 0.559
5.305ArgLeu: 5.305 ± 0.584
1.741ArgMet: 1.741 ± 0.383
2.57ArgAsn: 2.57 ± 0.429
2.736ArgPro: 2.736 ± 0.514
1.824ArgGln: 1.824 ± 0.394
4.642ArgArg: 4.642 ± 0.675
2.238ArgSer: 2.238 ± 0.586
3.399ArgThr: 3.399 ± 0.543
3.896ArgVal: 3.896 ± 0.576
1.078ArgTrp: 1.078 ± 0.31
2.155ArgTyr: 2.155 ± 0.297
0.0ArgXaa: 0.0 ± 0.0
Ser
6.052SerAla: 6.052 ± 0.79
0.829SerCys: 0.829 ± 0.268
3.316SerAsp: 3.316 ± 0.543
3.813SerGlu: 3.813 ± 0.6
2.238SerPhe: 2.238 ± 0.336
5.305SerGly: 5.305 ± 0.91
0.746SerHis: 0.746 ± 0.247
2.155SerIle: 2.155 ± 0.499
3.648SerLys: 3.648 ± 0.591
5.223SerLeu: 5.223 ± 0.494
1.741SerMet: 1.741 ± 0.429
1.741SerAsn: 1.741 ± 0.328
2.901SerPro: 2.901 ± 0.369
2.984SerGln: 2.984 ± 0.445
3.15SerArg: 3.15 ± 0.666
3.482SerSer: 3.482 ± 0.783
3.73SerThr: 3.73 ± 0.655
4.145SerVal: 4.145 ± 0.592
0.746SerTrp: 0.746 ± 0.225
1.492SerTyr: 1.492 ± 0.373
0.0SerXaa: 0.0 ± 0.0
Thr
6.134ThrAla: 6.134 ± 0.833
0.663ThrCys: 0.663 ± 0.247
3.813ThrAsp: 3.813 ± 0.407
3.316ThrGlu: 3.316 ± 0.54
1.824ThrPhe: 1.824 ± 0.425
5.14ThrGly: 5.14 ± 0.676
1.078ThrHis: 1.078 ± 0.309
3.316ThrIle: 3.316 ± 0.57
3.565ThrLys: 3.565 ± 0.578
5.223ThrLeu: 5.223 ± 0.612
1.326ThrMet: 1.326 ± 0.329
2.238ThrAsn: 2.238 ± 0.558
4.394ThrPro: 4.394 ± 0.588
1.741ThrGln: 1.741 ± 0.345
2.984ThrArg: 2.984 ± 0.563
3.067ThrSer: 3.067 ± 0.544
4.062ThrThr: 4.062 ± 0.613
4.808ThrVal: 4.808 ± 0.869
0.414ThrTrp: 0.414 ± 0.175
1.907ThrTyr: 1.907 ± 0.561
0.0ThrXaa: 0.0 ± 0.0
Val
6.466ValAla: 6.466 ± 0.76
1.243ValCys: 1.243 ± 0.321
3.233ValAsp: 3.233 ± 0.552
4.891ValGlu: 4.891 ± 0.577
2.736ValPhe: 2.736 ± 0.629
4.725ValGly: 4.725 ± 0.698
1.741ValHis: 1.741 ± 0.403
3.316ValIle: 3.316 ± 0.542
3.399ValLys: 3.399 ± 0.541
4.476ValLeu: 4.476 ± 0.645
1.907ValMet: 1.907 ± 0.458
2.984ValAsn: 2.984 ± 0.49
3.979ValPro: 3.979 ± 0.568
2.901ValGln: 2.901 ± 0.459
4.394ValArg: 4.394 ± 0.612
4.642ValSer: 4.642 ± 0.741
5.057ValThr: 5.057 ± 0.757
5.969ValVal: 5.969 ± 0.877
0.58ValTrp: 0.58 ± 0.233
1.409ValTyr: 1.409 ± 0.388
0.0ValXaa: 0.0 ± 0.0
Trp
1.658TrpAla: 1.658 ± 0.354
0.249TrpCys: 0.249 ± 0.151
0.912TrpAsp: 0.912 ± 0.309
0.58TrpGlu: 0.58 ± 0.194
0.414TrpPhe: 0.414 ± 0.235
0.995TrpGly: 0.995 ± 0.313
0.414TrpHis: 0.414 ± 0.171
0.663TrpIle: 0.663 ± 0.225
0.663TrpLys: 0.663 ± 0.261
1.078TrpLeu: 1.078 ± 0.35
0.829TrpMet: 0.829 ± 0.257
0.829TrpAsn: 0.829 ± 0.238
0.746TrpPro: 0.746 ± 0.288
0.829TrpGln: 0.829 ± 0.231
0.995TrpArg: 0.995 ± 0.284
0.746TrpSer: 0.746 ± 0.236
1.492TrpThr: 1.492 ± 0.331
1.161TrpVal: 1.161 ± 0.205
0.332TrpTrp: 0.332 ± 0.148
0.332TrpTyr: 0.332 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.155TyrAla: 2.155 ± 0.475
0.414TyrCys: 0.414 ± 0.175
1.824TyrAsp: 1.824 ± 0.398
1.575TyrGlu: 1.575 ± 0.408
0.995TyrPhe: 0.995 ± 0.285
2.487TyrGly: 2.487 ± 0.479
0.249TyrHis: 0.249 ± 0.161
1.658TyrIle: 1.658 ± 0.33
1.658TyrLys: 1.658 ± 0.301
2.404TyrLeu: 2.404 ± 0.39
0.249TyrMet: 0.249 ± 0.148
1.161TyrAsn: 1.161 ± 0.29
1.824TyrPro: 1.824 ± 0.33
1.575TyrGln: 1.575 ± 0.354
1.907TyrArg: 1.907 ± 0.415
1.658TyrSer: 1.658 ± 0.297
2.155TyrThr: 2.155 ± 0.529
1.658TyrVal: 1.658 ± 0.372
0.414TyrTrp: 0.414 ± 0.187
0.166TyrTyr: 0.166 ± 0.092
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12064 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski