Sphingomonas carotinifaciens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacter

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7R2A9|A0A1G7R2A9_9SPHN Lytic murein transglycosylase OS=Sphingomonas carotinifaciens OX=1166323 GN=SAMN05216557_11017 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.3ILVLASASVLAFATPALAQTVSNQSYY28 pKa = 8.56VQQIGNEE35 pKa = 3.8NAATVLQNDD44 pKa = 4.31DD45 pKa = 4.2TNVSQIFQASSNDD58 pKa = 3.45TALVEE63 pKa = 4.09MSGGNNQSLVLQQKK77 pKa = 8.45TDD79 pKa = 3.9FVSGRR84 pKa = 11.84NDD86 pKa = 3.02SQTRR90 pKa = 11.84MSGYY94 pKa = 9.89NGTSITSQSGEE105 pKa = 3.78GNYY108 pKa = 11.04ANVTFSPASGDD119 pKa = 3.61SSSLIMQDD127 pKa = 2.92GKK129 pKa = 11.5GNEE132 pKa = 4.51AIVASGDD139 pKa = 3.0WGNASVITQTFDD151 pKa = 2.78EE152 pKa = 5.17NYY154 pKa = 10.73ANVYY158 pKa = 8.93QDD160 pKa = 3.22GYY162 pKa = 10.04NNVSVVVQDD171 pKa = 4.12GLANTANVTQHH182 pKa = 5.92

Molecular weight: 19.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7EVF1|A0A1G7EVF1_9SPHN Murein hydrolase A OS=Sphingomonas carotinifaciens OX=1166323 GN=GGR49_001348 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3738

0

3738

1219373

29

3299

326.2

35.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.081 ± 0.067

0.705 ± 0.011

6.092 ± 0.033

4.915 ± 0.036

3.393 ± 0.028

9.157 ± 0.047

1.99 ± 0.018

4.654 ± 0.024

2.405 ± 0.03

9.717 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.021

2.419 ± 0.03

5.52 ± 0.033

3.073 ± 0.024

7.949 ± 0.041

4.795 ± 0.033

5.672 ± 0.043

7.488 ± 0.037

1.453 ± 0.02

2.189 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski