Amino acid dipepetide frequency for Sphingomonas carotinifaciens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.534AlaAla: 21.534 ± 0.22	1.073AlaCys: 1.073 ± 0.031	8.206AlaAsp: 8.206 ± 0.103	7.534AlaGlu: 7.534 ± 0.104	4.34AlaPhe: 4.34 ± 0.06	12.713AlaGly: 12.713 ± 0.162	2.469AlaHis: 2.469 ± 0.052	6.834AlaIle: 6.834 ± 0.083	3.482AlaLys: 3.482 ± 0.067	14.348AlaLeu: 14.348 ± 0.159	4.021AlaMet: 4.021 ± 0.07	3.123AlaAsn: 3.123 ± 0.058	7.167AlaPro: 7.167 ± 0.11	4.758AlaGln: 4.758 ± 0.064	10.947AlaArg: 10.947 ± 0.114	6.362AlaSer: 6.362 ± 0.07	7.798AlaThr: 7.798 ± 0.104	9.711AlaVal: 9.711 ± 0.106	1.766AlaTrp: 1.766 ± 0.044	2.623AlaTyr: 2.623 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.952CysAla: 0.952 ± 0.028	0.088CysCys: 0.088 ± 0.009	0.48CysAsp: 0.48 ± 0.018	0.321CysGlu: 0.321 ± 0.017	0.265CysPhe: 0.265 ± 0.016	0.774CysGly: 0.774 ± 0.028	0.192CysHis: 0.192 ± 0.014	0.309CysIle: 0.309 ± 0.015	0.109CysLys: 0.109 ± 0.01	0.641CysLeu: 0.641 ± 0.022	0.13CysMet: 0.13 ± 0.01	0.177CysAsn: 0.177 ± 0.013	0.408CysPro: 0.408 ± 0.02	0.175CysGln: 0.175 ± 0.014	0.549CysArg: 0.549 ± 0.024	0.356CysSer: 0.356 ± 0.017	0.403CysThr: 0.403 ± 0.02	0.481CysVal: 0.481 ± 0.02	0.099CysTrp: 0.099 ± 0.009	0.143CysTyr: 0.143 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.816AspAla: 8.816 ± 0.111	0.391AspCys: 0.391 ± 0.02	3.538AspAsp: 3.538 ± 0.072	3.099AspGlu: 3.099 ± 0.057	1.981AspPhe: 1.981 ± 0.041	5.96AspGly: 5.96 ± 0.103	1.405AspHis: 1.405 ± 0.04	2.587AspIle: 2.587 ± 0.056	1.37AspLys: 1.37 ± 0.036	5.864AspLeu: 5.864 ± 0.068	1.323AspMet: 1.323 ± 0.031	1.238AspAsn: 1.238 ± 0.047	3.918AspPro: 3.918 ± 0.056	1.744AspGln: 1.744 ± 0.038	5.554AspArg: 5.554 ± 0.08	2.064AspSer: 2.064 ± 0.056	3.105AspThr: 3.105 ± 0.055	4.214AspVal: 4.214 ± 0.061	1.152AspTrp: 1.152 ± 0.032	1.599AspTyr: 1.599 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	7.492GluAla: 7.492 ± 0.103	0.267GluCys: 0.267 ± 0.013	2.607GluAsp: 2.607 ± 0.053	2.477GluGlu: 2.477 ± 0.061	1.183GluPhe: 1.183 ± 0.033	4.356GluGly: 4.356 ± 0.069	1.087GluHis: 1.087 ± 0.029	2.37GluIle: 2.37 ± 0.047	1.385GluLys: 1.385 ± 0.035	4.292GluLeu: 4.292 ± 0.073	1.231GluMet: 1.231 ± 0.031	1.113GluAsn: 1.113 ± 0.033	2.5GluPro: 2.5 ± 0.053	1.991GluGln: 1.991 ± 0.042	5.053GluArg: 5.053 ± 0.07	1.864GluSer: 1.864 ± 0.039	2.938GluThr: 2.938 ± 0.053	3.356GluVal: 3.356 ± 0.06	0.726GluTrp: 0.726 ± 0.027	0.859GluTyr: 0.859 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.912PheAla: 4.912 ± 0.067	0.296PheCys: 0.296 ± 0.015	2.561PheAsp: 2.561 ± 0.05	1.769PheGlu: 1.769 ± 0.037	1.165PhePhe: 1.165 ± 0.038	3.558PheGly: 3.558 ± 0.065	0.709PheHis: 0.709 ± 0.024	1.18PheIle: 1.18 ± 0.033	0.668PheLys: 0.668 ± 0.028	2.902PheLeu: 2.902 ± 0.056	0.684PheMet: 0.684 ± 0.024	0.975PheAsn: 0.975 ± 0.037	1.41PhePro: 1.41 ± 0.036	0.892PheGln: 0.892 ± 0.028	2.381PheArg: 2.381 ± 0.045	1.792PheSer: 1.792 ± 0.044	2.036PheThr: 2.036 ± 0.039	2.661PheVal: 2.661 ± 0.049	0.498PheTrp: 0.498 ± 0.022	0.878PheTyr: 0.878 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	10.85GlyAla: 10.85 ± 0.129	0.778GlyCys: 0.778 ± 0.026	5.204GlyAsp: 5.204 ± 0.092	4.779GlyGlu: 4.779 ± 0.067	3.639GlyPhe: 3.639 ± 0.057	8.749GlyGly: 8.749 ± 0.142	1.855GlyHis: 1.855 ± 0.047	4.4GlyIle: 4.4 ± 0.06	2.537GlyLys: 2.537 ± 0.052	8.627GlyLeu: 8.627 ± 0.092	2.287GlyMet: 2.287 ± 0.047	2.257GlyAsn: 2.257 ± 0.067	3.827GlyPro: 3.827 ± 0.066	3.128GlyGln: 3.128 ± 0.05	7.463GlyArg: 7.463 ± 0.095	4.656GlySer: 4.656 ± 0.074	5.562GlyThr: 5.562 ± 0.115	6.826GlyVal: 6.826 ± 0.086	1.716GlyTrp: 1.716 ± 0.038	2.431GlyTyr: 2.431 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.678HisAla: 2.678 ± 0.059	0.194HisCys: 0.194 ± 0.014	1.36HisAsp: 1.36 ± 0.035	0.922HisGlu: 0.922 ± 0.031	0.704HisPhe: 0.704 ± 0.022	2.071HisGly: 2.071 ± 0.048	0.622HisHis: 0.622 ± 0.024	0.791HisIle: 0.791 ± 0.029	0.372HisLys: 0.372 ± 0.019	1.955HisLeu: 1.955 ± 0.044	0.412HisMet: 0.412 ± 0.019	0.422HisAsn: 0.422 ± 0.02	1.315HisPro: 1.315 ± 0.035	0.556HisGln: 0.556 ± 0.022	1.652HisArg: 1.652 ± 0.04	0.767HisSer: 0.767 ± 0.025	0.707HisThr: 0.707 ± 0.022	1.511HisVal: 1.511 ± 0.037	0.388HisTrp: 0.388 ± 0.018	0.5HisTyr: 0.5 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.771IleAla: 7.771 ± 0.09	0.359IleCys: 0.359 ± 0.019	3.794IleAsp: 3.794 ± 0.062	2.974IleGlu: 2.974 ± 0.056	1.277IlePhe: 1.277 ± 0.033	5.085IleGly: 5.085 ± 0.065	0.844IleHis: 0.844 ± 0.026	1.553IleIle: 1.553 ± 0.036	0.946IleLys: 0.946 ± 0.03	3.731IleLeu: 3.731 ± 0.059	0.759IleMet: 0.759 ± 0.026	1.165IleAsn: 1.165 ± 0.033	2.017IlePro: 2.017 ± 0.048	1.099IleGln: 1.099 ± 0.033	3.232IleArg: 3.232 ± 0.05	1.997IleSer: 1.997 ± 0.039	2.496IleThr: 2.496 ± 0.051	4.057IleVal: 4.057 ± 0.069	0.507IleTrp: 0.507 ± 0.023	0.878IleTyr: 0.878 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.398LysAla: 3.398 ± 0.064	0.093LysCys: 0.093 ± 0.009	1.235LysAsp: 1.235 ± 0.037	0.837LysGlu: 0.837 ± 0.025	0.649LysPhe: 0.649 ± 0.024	2.209LysGly: 2.209 ± 0.048	0.398LysHis: 0.398 ± 0.018	1.045LysIle: 1.045 ± 0.029	0.79LysLys: 0.79 ± 0.032	2.473LysLeu: 2.473 ± 0.056	0.602LysMet: 0.602 ± 0.025	0.56LysAsn: 0.56 ± 0.025	1.747LysPro: 1.747 ± 0.036	0.764LysGln: 0.764 ± 0.031	2.051LysArg: 2.051 ± 0.042	1.17LysSer: 1.17 ± 0.033	1.463LysThr: 1.463 ± 0.036	1.802LysVal: 1.802 ± 0.037	0.308LysTrp: 0.308 ± 0.018	0.458LysTyr: 0.458 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.699LeuAla: 14.699 ± 0.163	0.725LeuCys: 0.725 ± 0.023	6.25LeuAsp: 6.25 ± 0.091	4.111LeuGlu: 4.111 ± 0.072	3.562LeuPhe: 3.562 ± 0.061	8.404LeuGly: 8.404 ± 0.101	1.853LeuHis: 1.853 ± 0.042	4.419LeuIle: 4.419 ± 0.067	2.512LeuLys: 2.512 ± 0.048	9.069LeuLeu: 9.069 ± 0.14	2.022LeuMet: 2.022 ± 0.044	2.394LeuAsn: 2.394 ± 0.058	5.767LeuPro: 5.767 ± 0.073	2.338LeuGln: 2.338 ± 0.043	7.071LeuArg: 7.071 ± 0.092	5.653LeuSer: 5.653 ± 0.072	5.605LeuThr: 5.605 ± 0.065	7.47LeuVal: 7.47 ± 0.087	1.249LeuTrp: 1.249 ± 0.04	1.999LeuTyr: 1.999 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	3.224MetAla: 3.224 ± 0.057	0.12MetCys: 0.12 ± 0.01	1.019MetAsp: 1.019 ± 0.03	0.902MetGlu: 0.902 ± 0.029	0.595MetPhe: 0.595 ± 0.023	1.83MetGly: 1.83 ± 0.041	0.407MetHis: 0.407 ± 0.017	1.265MetIle: 1.265 ± 0.033	0.806MetLys: 0.806 ± 0.024	2.65MetLeu: 2.65 ± 0.057	0.65MetMet: 0.65 ± 0.026	0.633MetAsn: 0.633 ± 0.024	1.489MetPro: 1.489 ± 0.038	0.679MetGln: 0.679 ± 0.026	1.93MetArg: 1.93 ± 0.042	1.236MetSer: 1.236 ± 0.031	1.817MetThr: 1.817 ± 0.038	1.616MetVal: 1.616 ± 0.037	0.21MetTrp: 0.21 ± 0.013	0.254MetTyr: 0.254 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.281AsnAla: 3.281 ± 0.062	0.203AsnCys: 0.203 ± 0.015	1.388AsnAsp: 1.388 ± 0.042	0.973AsnGlu: 0.973 ± 0.032	0.889AsnPhe: 0.889 ± 0.037	2.414AsnGly: 2.414 ± 0.069	0.452AsnHis: 0.452 ± 0.021	1.115AsnIle: 1.115 ± 0.037	0.555AsnLys: 0.555 ± 0.022	2.341AsnLeu: 2.341 ± 0.055	0.455AsnMet: 0.455 ± 0.02	0.68AsnAsn: 0.68 ± 0.033	1.648AsnPro: 1.648 ± 0.043	0.71AsnGln: 0.71 ± 0.027	1.888AsnArg: 1.888 ± 0.038	1.09AsnSer: 1.09 ± 0.054	1.248AsnThr: 1.248 ± 0.046	1.808AsnVal: 1.808 ± 0.04	0.398AsnTrp: 0.398 ± 0.019	0.652AsnTyr: 0.652 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	8.111ProAla: 8.111 ± 0.112	0.318ProCys: 0.318 ± 0.017	3.941ProAsp: 3.941 ± 0.056	3.181ProGlu: 3.181 ± 0.058	1.902ProPhe: 1.902 ± 0.042	5.079ProGly: 5.079 ± 0.07	1.074ProHis: 1.074 ± 0.032	2.449ProIle: 2.449 ± 0.045	1.256ProLys: 1.256 ± 0.037	4.992ProLeu: 4.992 ± 0.073	1.224ProMet: 1.224 ± 0.033	1.274ProAsn: 1.274 ± 0.038	3.094ProPro: 3.094 ± 0.073	1.651ProGln: 1.651 ± 0.04	3.571ProArg: 3.571 ± 0.058	2.681ProSer: 2.681 ± 0.053	2.822ProThr: 2.822 ± 0.056	4.756ProVal: 4.756 ± 0.069	0.722ProTrp: 0.722 ± 0.026	1.102ProTyr: 1.102 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.483GlnAla: 4.483 ± 0.067	0.164GlnCys: 0.164 ± 0.011	1.529GlnAsp: 1.529 ± 0.036	1.128GlnGlu: 1.128 ± 0.033	1.015GlnPhe: 1.015 ± 0.031	2.732GlnGly: 2.732 ± 0.049	0.589GlnHis: 0.589 ± 0.022	1.502GlnIle: 1.502 ± 0.042	0.691GlnLys: 0.691 ± 0.024	2.841GlnLeu: 2.841 ± 0.044	0.755GlnMet: 0.755 ± 0.025	0.72GlnAsn: 0.72 ± 0.027	1.917GlnPro: 1.917 ± 0.038	1.21GlnGln: 1.21 ± 0.039	2.616GlnArg: 2.616 ± 0.048	1.546GlnSer: 1.546 ± 0.039	1.736GlnThr: 1.736 ± 0.048	2.496GlnVal: 2.496 ± 0.048	0.435GlnTrp: 0.435 ± 0.019	0.618GlnTyr: 0.618 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	9.924ArgAla: 9.924 ± 0.098	0.505ArgCys: 0.505 ± 0.02	4.82ArgAsp: 4.82 ± 0.056	3.924ArgGlu: 3.924 ± 0.074	3.396ArgPhe: 3.396 ± 0.056	5.735ArgGly: 5.735 ± 0.081	1.91ArgHis: 1.91 ± 0.045	4.324ArgIle: 4.324 ± 0.06	1.602ArgLys: 1.602 ± 0.044	8.739ArgLeu: 8.739 ± 0.111	2.141ArgMet: 2.141 ± 0.047	1.835ArgAsn: 1.835 ± 0.04	4.24ArgPro: 4.24 ± 0.076	2.577ArgGln: 2.577 ± 0.045	6.675ArgArg: 6.675 ± 0.108	3.798ArgSer: 3.798 ± 0.063	4.132ArgThr: 4.132 ± 0.064	5.601ArgVal: 5.601 ± 0.07	1.492ArgTrp: 1.492 ± 0.038	2.121ArgTyr: 2.121 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.09SerAla: 6.09 ± 0.083	0.341SerCys: 0.341 ± 0.017	2.862SerAsp: 2.862 ± 0.055	2.087SerGlu: 2.087 ± 0.044	2.009SerPhe: 2.009 ± 0.04	5.135SerGly: 5.135 ± 0.075	0.855SerHis: 0.855 ± 0.026	2.433SerIle: 2.433 ± 0.043	1.108SerLys: 1.108 ± 0.031	4.655SerLeu: 4.655 ± 0.069	1.056SerMet: 1.056 ± 0.032	1.235SerAsn: 1.235 ± 0.036	2.768SerPro: 2.768 ± 0.047	1.301SerGln: 1.301 ± 0.036	3.354SerArg: 3.354 ± 0.055	2.378SerSer: 2.378 ± 0.052	2.577SerThr: 2.577 ± 0.063	3.654SerVal: 3.654 ± 0.06	0.717SerTrp: 0.717 ± 0.026	1.337SerTyr: 1.337 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	7.167ThrAla: 7.167 ± 0.093	0.356ThrCys: 0.356 ± 0.016	3.104ThrAsp: 3.104 ± 0.052	2.209ThrGlu: 2.209 ± 0.045	1.823ThrPhe: 1.823 ± 0.044	5.855ThrGly: 5.855 ± 0.089	0.989ThrHis: 0.989 ± 0.026	3.128ThrIle: 3.128 ± 0.062	1.182ThrLys: 1.182 ± 0.036	6.448ThrLeu: 6.448 ± 0.082	1.258ThrMet: 1.258 ± 0.032	1.351ThrAsn: 1.351 ± 0.044	3.884ThrPro: 3.884 ± 0.064	1.671ThrGln: 1.671 ± 0.04	4.163ThrArg: 4.163 ± 0.062	2.617ThrSer: 2.617 ± 0.06	3.127ThrThr: 3.127 ± 0.075	4.563ThrVal: 4.563 ± 0.099	0.66ThrTrp: 0.66 ± 0.025	1.167ThrTyr: 1.167 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	11.099ValAla: 11.099 ± 0.137	0.464ValCys: 0.464 ± 0.019	4.609ValAsp: 4.609 ± 0.055	4.385ValGlu: 4.385 ± 0.067	2.163ValPhe: 2.163 ± 0.043	5.73ValGly: 5.73 ± 0.087	1.375ValHis: 1.375 ± 0.035	3.463ValIle: 3.463 ± 0.055	1.8ValLys: 1.8 ± 0.045	6.84ValLeu: 6.84 ± 0.084	1.61ValMet: 1.61 ± 0.04	1.967ValAsn: 1.967 ± 0.054	4.284ValPro: 4.284 ± 0.056	2.161ValGln: 2.161 ± 0.039	5.79ValArg: 5.79 ± 0.069	3.954ValSer: 3.954 ± 0.062	5.088ValThr: 5.088 ± 0.094	5.909ValVal: 5.909 ± 0.088	0.836ValTrp: 0.836 ± 0.031	1.349ValTyr: 1.349 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.422TrpAla: 1.422 ± 0.039	0.13TrpCys: 0.13 ± 0.01	0.782TrpAsp: 0.782 ± 0.027	0.52TrpGlu: 0.52 ± 0.022	0.571TrpPhe: 0.571 ± 0.022	0.984TrpGly: 0.984 ± 0.029	0.362TrpHis: 0.362 ± 0.016	0.651TrpIle: 0.651 ± 0.025	0.389TrpLys: 0.389 ± 0.018	1.689TrpLeu: 1.689 ± 0.047	0.344TrpMet: 0.344 ± 0.018	0.447TrpAsn: 0.447 ± 0.021	0.759TrpPro: 0.759 ± 0.026	0.618TrpGln: 0.618 ± 0.024	1.503TrpArg: 1.503 ± 0.039	0.915TrpSer: 0.915 ± 0.03	0.977TrpThr: 0.977 ± 0.032	0.841TrpVal: 0.841 ± 0.026	0.304TrpTrp: 0.304 ± 0.018	0.323TrpTyr: 0.323 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.904TyrAla: 2.904 ± 0.049	0.188TyrCys: 0.188 ± 0.013	1.634TyrAsp: 1.634 ± 0.046	1.014TyrGlu: 1.014 ± 0.035	0.801TyrPhe: 0.801 ± 0.03	2.198TyrGly: 2.198 ± 0.047	0.453TyrHis: 0.453 ± 0.019	0.723TyrIle: 0.723 ± 0.029	0.49TyrLys: 0.49 ± 0.023	2.076TyrLeu: 2.076 ± 0.047	0.367TyrMet: 0.367 ± 0.017	0.623TyrAsn: 0.623 ± 0.03	1.038TyrPro: 1.038 ± 0.027	0.704TyrGln: 0.704 ± 0.026	2.046TyrArg: 2.046 ± 0.049	1.056TyrSer: 1.056 ± 0.035	1.086TyrThr: 1.086 ± 0.036	1.542TyrVal: 1.542 ± 0.038	0.349TyrTrp: 0.349 ± 0.017	0.6TyrTyr: 0.6 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3738 proteins (1219373 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---