Treponema caldarium (strain ATCC 51460 / DSM 7334 / H1) (Spirochaeta caldaria)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema caldarium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8F3S8|F8F3S8_TRECH Alpha-2-macroglobulin domain protein OS=Treponema caldarium (strain ATCC 51460 / DSM 7334 / H1) OX=744872 GN=Spica_2336 PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.23KK3 pKa = 10.15YY4 pKa = 10.59VCDD7 pKa = 3.52VCGYY11 pKa = 10.08IYY13 pKa = 10.73DD14 pKa = 4.13PAEE17 pKa = 3.94GDD19 pKa = 3.28KK20 pKa = 11.39DD21 pKa = 4.26GGIQPGTPFEE31 pKa = 4.39SLPADD36 pKa = 4.29WVCPMCGASKK46 pKa = 11.06DD47 pKa = 3.82DD48 pKa = 4.45FSPVDD53 pKa = 3.24

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8EWM0|F8EWM0_TRECH Uncharacterized protein OS=Treponema caldarium (strain ATCC 51460 / DSM 7334 / H1) OX=744872 GN=Spica_0011 PE=4 SV=1
MM1 pKa = 7.26LQNGLPVNRR10 pKa = 11.84IAFTFARR17 pKa = 11.84KK18 pKa = 9.56YY19 pKa = 10.79GNAVQRR25 pKa = 11.84NRR27 pKa = 11.84SRR29 pKa = 11.84RR30 pKa = 11.84LSRR33 pKa = 11.84EE34 pKa = 3.73VYY36 pKa = 9.86RR37 pKa = 11.84LLKK40 pKa = 10.65ARR42 pKa = 11.84LCSGFDD48 pKa = 3.6LVLLVYY54 pKa = 10.18PGKK57 pKa = 9.54DD58 pKa = 2.88TFNEE62 pKa = 3.71RR63 pKa = 11.84MEE65 pKa = 4.08QLRR68 pKa = 11.84TLFQKK73 pKa = 11.11ADD75 pKa = 3.72LFVDD79 pKa = 3.81KK80 pKa = 10.58TT81 pKa = 3.65

Molecular weight: 9.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2754

0

2754

973006

31

3676

353.3

39.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.283 ± 0.055

0.882 ± 0.014

5.15 ± 0.033

6.535 ± 0.052

4.365 ± 0.034

7.073 ± 0.046

1.875 ± 0.021

7.452 ± 0.043

5.503 ± 0.043

10.543 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.019

3.736 ± 0.039

4.346 ± 0.035

3.765 ± 0.03

5.37 ± 0.044

6.297 ± 0.035

5.46 ± 0.053

6.485 ± 0.038

1.155 ± 0.019

3.469 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski