Pseudomonas resinovorans NBRC 106553

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas aeruginosa group; Pseudomonas resinovorans

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6AKA0|S6AKA0_PSERE Phosphate-binding protein OS=Pseudomonas resinovorans NBRC 106553 OX=1245471 GN=pstS PE=3 SV=1
MM1 pKa = 7.3TLFRR5 pKa = 11.84SSPLFLGAPLLGALLNAGPASADD28 pKa = 3.01SGQYY32 pKa = 10.11PNVGMDD38 pKa = 3.28YY39 pKa = 11.04DD40 pKa = 3.76ATFQYY45 pKa = 11.32DD46 pKa = 3.65FTRR49 pKa = 11.84ADD51 pKa = 3.12HH52 pKa = 5.84STPGTSRR59 pKa = 11.84TTTDD63 pKa = 3.89GYY65 pKa = 10.42PDD67 pKa = 3.39INATFYY73 pKa = 11.24LRR75 pKa = 11.84FSTDD79 pKa = 2.97SQIHH83 pKa = 5.86LTTEE87 pKa = 4.43LNPINPPNDD96 pKa = 3.56GEE98 pKa = 4.55DD99 pKa = 3.12RR100 pKa = 11.84FFEE103 pKa = 4.76DD104 pKa = 4.1VGLQVNEE111 pKa = 4.28LAYY114 pKa = 10.29DD115 pKa = 4.01YY116 pKa = 11.17QSSRR120 pKa = 11.84YY121 pKa = 9.12SFSLGLVQVPIGRR134 pKa = 11.84AQDD137 pKa = 3.69AAPGLYY143 pKa = 9.88TSDD146 pKa = 3.47FVAVYY151 pKa = 10.39DD152 pKa = 4.31LDD154 pKa = 4.56GMLGGTFAYY163 pKa = 10.26RR164 pKa = 11.84FFGDD168 pKa = 3.22QLGVIEE174 pKa = 4.93TDD176 pKa = 3.04LTLYY180 pKa = 10.62RR181 pKa = 11.84QDD183 pKa = 3.05TSVLSRR189 pKa = 11.84PYY191 pKa = 9.69FQSGQQVDD199 pKa = 3.97RR200 pKa = 11.84NDD202 pKa = 3.72GGPANNGKK210 pKa = 9.73INSYY214 pKa = 10.37AVAVNWLAIPALPFLEE230 pKa = 4.47LQAGQMRR237 pKa = 11.84NQGGDD242 pKa = 3.1FDD244 pKa = 5.32GVDD247 pKa = 3.72GSADD251 pKa = 3.61DD252 pKa = 4.28EE253 pKa = 4.89VVNLASLRR261 pKa = 11.84YY262 pKa = 8.35IYY264 pKa = 10.36AVPRR268 pKa = 11.84STDD271 pKa = 3.05LDD273 pKa = 3.89TTLSGQYY280 pKa = 10.32LDD282 pKa = 3.41VVPFIEE288 pKa = 4.67YY289 pKa = 10.82ADD291 pKa = 3.73VDD293 pKa = 3.96NEE295 pKa = 4.18GAIPGNDD302 pKa = 2.99TRR304 pKa = 11.84YY305 pKa = 8.68LTTSLTLDD313 pKa = 3.25YY314 pKa = 10.93GLWAFGLTRR323 pKa = 11.84TDD325 pKa = 2.61KK326 pKa = 10.8RR327 pKa = 11.84RR328 pKa = 11.84PNDD331 pKa = 3.31TGGGDD336 pKa = 3.1THH338 pKa = 7.55DD339 pKa = 4.23YY340 pKa = 11.22LNEE343 pKa = 3.98LSVTYY348 pKa = 10.75ALTGQLSIGVSAGTQKK364 pKa = 10.66QDD366 pKa = 3.45GEE368 pKa = 4.25NDD370 pKa = 3.67NILGLAITYY379 pKa = 10.41SGGYY383 pKa = 8.85

Molecular weight: 41.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6ABZ8|S6ABZ8_PSERE Dihydrofolate reductase OS=Pseudomonas resinovorans NBRC 106553 OX=1245471 GN=folA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5795

0

5795

1892167

43

5569

326.5

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.405 ± 0.046

0.983 ± 0.013

5.44 ± 0.031

5.952 ± 0.038

3.645 ± 0.023

8.401 ± 0.044

2.195 ± 0.018

4.456 ± 0.026

3.151 ± 0.029

11.987 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.021

2.896 ± 0.036

4.956 ± 0.027

4.327 ± 0.027

6.83 ± 0.045

5.524 ± 0.033

4.582 ± 0.048

7.083 ± 0.027

1.437 ± 0.015

2.501 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski