Photobacterium jeanii
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A178KHI3|A0A178KHI3_9GAMM Flagellar protein FliL OS=Photobacterium jeanii OX=858640 GN=A3K86_09800 PE=3 SV=1
MM1 pKa = 7.55 LSKK4 pKa = 10.79 FEE6 pKa = 4.43 QVQQQWGGTSDD17 pKa = 5.67 VIDD20 pKa = 3.36 HH21 pKa = 6.86 WLLSRR26 pKa = 11.84 QQLLIDD32 pKa = 3.65 YY33 pKa = 8.05 CKK35 pKa = 10.56 LAGLPPFDD43 pKa = 4.47 NSNNQLPEE51 pKa = 4.0 SSQLQLFSQQLVDD64 pKa = 4.39 YY65 pKa = 10.48 ISAGHH70 pKa = 5.71 FKK72 pKa = 10.39 IYY74 pKa = 10.87 DD75 pKa = 3.38 MVMARR80 pKa = 11.84 WNDD83 pKa = 3.24 TGYY86 pKa = 11.1 SPTEE90 pKa = 5.04 DD91 pKa = 3.21 ISQLYY96 pKa = 10.21 LKK98 pKa = 8.96 ITQTTDD104 pKa = 3.04 PLLNFADD111 pKa = 5.3 RR112 pKa = 11.84 YY113 pKa = 10.58 CDD115 pKa = 3.82 DD116 pKa = 4.69 NEE118 pKa = 4.72 ALDD121 pKa = 5.04 SLDD124 pKa = 5.21 DD125 pKa = 4.39 DD126 pKa = 5.18 LSVIGQLMEE135 pKa = 3.76 QRR137 pKa = 11.84 FALEE141 pKa = 4.04 DD142 pKa = 3.4 QLIEE146 pKa = 5.01 LISEE150 pKa = 4.38 SLACPPGAA158 pKa = 4.46
Molecular weight: 18.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.63
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A178K7J9|A0A178K7J9_9GAMM Arylsulfatase OS=Photobacterium jeanii OX=858640 GN=A3K86_16745 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 ATINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4290
0
4290
1410523
35
3634
328.8
36.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.216 ± 0.041
1.059 ± 0.013
5.542 ± 0.037
6.136 ± 0.032
4.037 ± 0.026
6.851 ± 0.041
2.307 ± 0.022
6.055 ± 0.027
5.064 ± 0.032
10.29 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.75 ± 0.022
4.181 ± 0.026
3.982 ± 0.022
4.952 ± 0.038
4.491 ± 0.031
6.306 ± 0.032
5.463 ± 0.026
7.041 ± 0.034
1.258 ± 0.015
3.019 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here