Adana virus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5B2U6|A0A0C5B2U6_9VIRU Nonstructural protein OS=Adana virus OX=1611877 PE=4 SV=1
MM1 pKa = 7.13 SLKK4 pKa = 10.18 YY5 pKa = 10.51 LCDD8 pKa = 5.33 RR9 pKa = 11.84 ITPTYY14 pKa = 9.28 DD15 pKa = 3.14 YY16 pKa = 10.35 PVIGEE21 pKa = 4.2 VVVSFEE27 pKa = 4.29 AVNSQVDD34 pKa = 4.35 LPVCVYY40 pKa = 11.34 DD41 pKa = 4.71 DD42 pKa = 5.83 LEE44 pKa = 5.32 FEE46 pKa = 4.36 LAGFRR51 pKa = 11.84 LSLAYY56 pKa = 9.85 KK57 pKa = 7.77 NTLYY61 pKa = 10.93 EE62 pKa = 4.9 FIDD65 pKa = 3.64 NDD67 pKa = 3.96 EE68 pKa = 4.55 IPWRR72 pKa = 11.84 WGPHH76 pKa = 3.62 MWEE79 pKa = 4.26 SRR81 pKa = 11.84 VTMDD85 pKa = 5.58 EE86 pKa = 4.03 IPLLDD91 pKa = 3.74 SLMKK95 pKa = 9.94 TFQGLPVTDD104 pKa = 4.51 LMDD107 pKa = 4.55 PRR109 pKa = 11.84 LHH111 pKa = 5.96 FTRR114 pKa = 11.84 NALSWPIGRR123 pKa = 11.84 PSLKK127 pKa = 10.13 AFRR130 pKa = 11.84 ILYY133 pKa = 7.11 PCRR136 pKa = 11.84 FVGFSCSKK144 pKa = 10.72 RR145 pKa = 11.84 EE146 pKa = 3.6 LAEE149 pKa = 4.21 YY150 pKa = 10.15 IFEE153 pKa = 4.2 VTKK156 pKa = 10.76 AVNLEE161 pKa = 4.06 CGIVQMAKK169 pKa = 9.93 MIRR172 pKa = 11.84 STAQSMGVQEE182 pKa = 4.62 SLVPCKK188 pKa = 11.02 DD189 pKa = 3.26 MVKK192 pKa = 10.06 CICILQFIKK201 pKa = 10.06 MIKK204 pKa = 10.07 GFQLDD209 pKa = 3.32 KK210 pKa = 10.61 TRR212 pKa = 11.84 NVLFGGALIPFLHH225 pKa = 6.95 ACTEE229 pKa = 3.85 ALTRR233 pKa = 11.84 DD234 pKa = 4.05 FPGLWRR240 pKa = 11.84 AWNPQADD247 pKa = 3.82 IMDD250 pKa = 6.47 LITADD255 pKa = 4.65 PYY257 pKa = 11.76 DD258 pKa = 4.11 SGISTDD264 pKa = 3.92 SEE266 pKa = 3.96 FDD268 pKa = 3.15 SDD270 pKa = 4.69 IEE272 pKa = 4.3 VV273 pKa = 3.14
Molecular weight: 31.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.683
IPC2_protein 4.762
IPC_protein 4.685
Toseland 4.533
ProMoST 4.813
Dawson 4.647
Bjellqvist 4.8
Wikipedia 4.533
Rodwell 4.533
Grimsley 4.444
Solomon 4.647
Lehninger 4.609
Nozaki 4.762
DTASelect 4.952
Thurlkill 4.546
EMBOSS 4.558
Sillero 4.813
Patrickios 3.694
IPC_peptide 4.647
IPC2_peptide 4.8
IPC2.peptide.svr19 4.783
Protein with the highest isoelectric point:
>tr|A0A0C5ARY4|A0A0C5ARY4_9VIRU Glycoprotein complex OS=Adana virus OX=1611877 PE=4 SV=1
MM1 pKa = 7.9 SSQEE5 pKa = 3.82 DD6 pKa = 3.8 FARR9 pKa = 11.84 IAVEE13 pKa = 4.14 FANEE17 pKa = 3.84 AVDD20 pKa = 3.66 TNQILEE26 pKa = 4.31 LVRR29 pKa = 11.84 EE30 pKa = 4.56 FAYY33 pKa = 10.2 QGYY36 pKa = 9.31 DD37 pKa = 2.91 AARR40 pKa = 11.84 VIEE43 pKa = 4.02 LVRR46 pKa = 11.84 SKK48 pKa = 11.18 GGEE51 pKa = 3.52 NWKK54 pKa = 10.51 NDD56 pKa = 3.3 VKK58 pKa = 10.21 TLIVIALTRR67 pKa = 11.84 GNKK70 pKa = 8.56 PSKK73 pKa = 9.73 ILSKK77 pKa = 8.12 MTPDD81 pKa = 3.18 AGRR84 pKa = 11.84 KK85 pKa = 8.35 FRR87 pKa = 11.84 EE88 pKa = 3.59 LVMRR92 pKa = 11.84 YY93 pKa = 8.63 GLKK96 pKa = 10.28 SGNPGRR102 pKa = 11.84 DD103 pKa = 3.46 DD104 pKa = 3.39 LTLARR109 pKa = 11.84 IASAFAAWTVQAIKK123 pKa = 10.63 VVEE126 pKa = 4.26 QYY128 pKa = 11.52 LPVTGTAMDD137 pKa = 4.91 DD138 pKa = 3.85 LSPGYY143 pKa = 8.61 PRR145 pKa = 11.84 PMMHH149 pKa = 7.08 PCFAGLIDD157 pKa = 3.65 NTLPEE162 pKa = 4.03 EE163 pKa = 4.31 TYY165 pKa = 10.49 RR166 pKa = 11.84 AILRR170 pKa = 11.84 AHH172 pKa = 6.52 SLFLDD177 pKa = 3.48 QFSRR181 pKa = 11.84 TINPQMRR188 pKa = 11.84 GKK190 pKa = 9.67 PKK192 pKa = 10.72 SEE194 pKa = 3.83 VAKK197 pKa = 10.87 SFEE200 pKa = 4.22 QPLQAAVNSRR210 pKa = 11.84 FLSSEE215 pKa = 4.07 SKK217 pKa = 10.59 RR218 pKa = 11.84 KK219 pKa = 9.35 ILRR222 pKa = 11.84 STGIIDD228 pKa = 4.6 SNLKK232 pKa = 8.9 PAPAVEE238 pKa = 4.12 HH239 pKa = 6.1 AAKK242 pKa = 10.41 KK243 pKa = 9.26 FLEE246 pKa = 4.42 MAA248 pKa = 4.6
Molecular weight: 27.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.994
IPC2_protein 8.799
IPC_protein 8.756
Toseland 9.604
ProMoST 9.268
Dawson 9.809
Bjellqvist 9.472
Wikipedia 9.955
Rodwell 10.189
Grimsley 9.867
Solomon 9.853
Lehninger 9.823
Nozaki 9.604
DTASelect 9.458
Thurlkill 9.663
EMBOSS 9.999
Sillero 9.736
Patrickios 7.658
IPC_peptide 9.853
IPC2_peptide 7.922
IPC2.peptide.svr19 7.956
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3952
248
2096
988.0
111.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.452 ± 0.485
2.657 ± 0.772
5.541 ± 0.454
6.705 ± 0.349
4.352 ± 0.357
5.719 ± 0.201
2.429 ± 0.312
6.402 ± 0.148
5.896 ± 0.344
8.856 ± 0.375
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.214 ± 0.4
3.669 ± 0.363
4.352 ± 0.329
3.087 ± 0.092
5.693 ± 0.212
8.401 ± 0.433
5.845 ± 0.36
6.68 ± 0.359
1.265 ± 0.173
2.783 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here