Dragonfly larvae associated circular virus-3

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|W5U2P7|W5U2P7_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-3 OX=1454024 PE=4 SV=1
MM1 pKa = 7.69ANRR4 pKa = 11.84RR5 pKa = 11.84SRR7 pKa = 11.84GWCFTLNNYY16 pKa = 7.82TPEE19 pKa = 5.19HH20 pKa = 6.17EE21 pKa = 4.65ALLAAVPCAYY31 pKa = 9.85MIFGRR36 pKa = 11.84EE37 pKa = 3.71VGANGTPHH45 pKa = 6.6LQGFVYY51 pKa = 9.95FPNAKK56 pKa = 8.63TFNGAKK62 pKa = 9.88AVLPAGCHH70 pKa = 6.2LEE72 pKa = 3.81AAMGSVAQNVEE83 pKa = 4.3YY84 pKa = 10.41CSKK87 pKa = 11.09DD88 pKa = 2.85GDD90 pKa = 3.77VEE92 pKa = 4.31EE93 pKa = 5.04RR94 pKa = 11.84GEE96 pKa = 4.28RR97 pKa = 11.84PLSAAEE103 pKa = 4.24KK104 pKa = 10.0GASEE108 pKa = 3.97AQRR111 pKa = 11.84WKK113 pKa = 10.55DD114 pKa = 3.22ARR116 pKa = 11.84LAAVSGDD123 pKa = 3.35IADD126 pKa = 4.08VPDD129 pKa = 5.37DD130 pKa = 3.51IYY132 pKa = 10.94IRR134 pKa = 11.84YY135 pKa = 9.04YY136 pKa = 9.69RR137 pKa = 11.84TLKK140 pKa = 10.48EE141 pKa = 3.53ISKK144 pKa = 10.57DD145 pKa = 3.11HH146 pKa = 6.28MARR149 pKa = 11.84PDD151 pKa = 3.74GLDD154 pKa = 3.59GVCGLWLYY162 pKa = 10.93RR163 pKa = 11.84SAGTGKK169 pKa = 9.94SRR171 pKa = 11.84YY172 pKa = 8.88AQEE175 pKa = 4.2QFPEE179 pKa = 4.74HH180 pKa = 5.99YY181 pKa = 10.17MKK183 pKa = 8.5MTNKK187 pKa = 9.21WCDD190 pKa = 3.2GYY192 pKa = 11.06QGQDD196 pKa = 3.36VVVMDD201 pKa = 6.11DD202 pKa = 3.94MDD204 pKa = 5.44PDD206 pKa = 4.17HH207 pKa = 7.17ACLRR211 pKa = 11.84HH212 pKa = 5.75HH213 pKa = 7.46LKK215 pKa = 10.36RR216 pKa = 11.84WADD219 pKa = 3.79RR220 pKa = 11.84YY221 pKa = 10.38PFIGEE226 pKa = 4.28TKK228 pKa = 10.22GGAISIRR235 pKa = 11.84PKK237 pKa = 10.55KK238 pKa = 10.31FVVTSQYY245 pKa = 11.01SIEE248 pKa = 4.84DD249 pKa = 3.33MFKK252 pKa = 10.94NRR254 pKa = 11.84DD255 pKa = 3.08GTLDD259 pKa = 3.59VEE261 pKa = 4.72TVAAIRR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84FEE271 pKa = 3.87VKK273 pKa = 10.28RR274 pKa = 11.84FGVNAFNPYY283 pKa = 10.39NII285 pKa = 4.5

Molecular weight:
32.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|W5U2P7|W5U2P7_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-3 OX=1454024 PE=4 SV=1
MM1 pKa = 7.2VLGKK5 pKa = 10.08RR6 pKa = 11.84GRR8 pKa = 11.84PGSMKK13 pKa = 10.27YY14 pKa = 9.62VPRR17 pKa = 11.84YY18 pKa = 9.15AKK20 pKa = 10.36RR21 pKa = 11.84ITRR24 pKa = 11.84GPYY27 pKa = 8.77VPRR30 pKa = 11.84GLTLRR35 pKa = 11.84PNNASVARR43 pKa = 11.84QVEE46 pKa = 4.56MKK48 pKa = 10.39FVDD51 pKa = 3.79ISPTFALDD59 pKa = 3.69FATPYY64 pKa = 10.43HH65 pKa = 6.63INLLNGIATGSDD77 pKa = 3.48LYY79 pKa = 11.14QRR81 pKa = 11.84VGRR84 pKa = 11.84SVYY87 pKa = 9.64MKK89 pKa = 10.2NLHH92 pKa = 7.22IDD94 pKa = 4.17FQCQNINTGSSTPDD108 pKa = 2.91LEE110 pKa = 4.18MRR112 pKa = 11.84VLVVLDD118 pKa = 3.78KK119 pKa = 11.27APNGAALPVLADD131 pKa = 3.9FLRR134 pKa = 11.84EE135 pKa = 3.69INAAGAATTNLNSHH149 pKa = 6.02QNTNNRR155 pKa = 11.84QRR157 pKa = 11.84FKK159 pKa = 11.29FLFDD163 pKa = 3.62KK164 pKa = 10.97KK165 pKa = 10.61IGIPPWNVGATTTGAPALNMVDD187 pKa = 3.78VYY189 pKa = 9.49WRR191 pKa = 11.84KK192 pKa = 9.81HH193 pKa = 4.72IKK195 pKa = 9.01CTTRR199 pKa = 11.84CSSLEE204 pKa = 3.93KK205 pKa = 10.46GHH207 pKa = 6.85RR208 pKa = 11.84CQKK211 pKa = 9.97SRR213 pKa = 11.84TMVYY217 pKa = 8.44TT218 pKa = 3.93

Molecular weight:
24.4 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

503

218

285

251.5

28.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.344 ± 1.036

2.187 ± 0.236

5.765 ± 1.097

4.175 ± 1.568

3.976 ± 0.205

7.753 ± 0.585

2.584 ± 0.195

4.175 ± 0.276

5.765 ± 0.133

6.561 ± 1.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.181 ± 0.02

5.567 ± 1.188

5.169 ± 0.532

2.982 ± 0.153

7.952 ± 0.204

4.573 ± 0.317

5.567 ± 1.495

6.958 ± 0.255

1.392 ± 0.318

4.374 ± 0.472

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski