Dragonfly larvae associated circular virus-3
Average proteome isoelectric point is 8.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U2P7|W5U2P7_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-3 OX=1454024 PE=4 SV=1
MM1 pKa = 7.69 ANRR4 pKa = 11.84 RR5 pKa = 11.84 SRR7 pKa = 11.84 GWCFTLNNYY16 pKa = 7.82 TPEE19 pKa = 5.19 HH20 pKa = 6.17 EE21 pKa = 4.65 ALLAAVPCAYY31 pKa = 9.85 MIFGRR36 pKa = 11.84 EE37 pKa = 3.71 VGANGTPHH45 pKa = 6.6 LQGFVYY51 pKa = 9.95 FPNAKK56 pKa = 8.63 TFNGAKK62 pKa = 9.88 AVLPAGCHH70 pKa = 6.2 LEE72 pKa = 3.81 AAMGSVAQNVEE83 pKa = 4.3 YY84 pKa = 10.41 CSKK87 pKa = 11.09 DD88 pKa = 2.85 GDD90 pKa = 3.77 VEE92 pKa = 4.31 EE93 pKa = 5.04 RR94 pKa = 11.84 GEE96 pKa = 4.28 RR97 pKa = 11.84 PLSAAEE103 pKa = 4.24 KK104 pKa = 10.0 GASEE108 pKa = 3.97 AQRR111 pKa = 11.84 WKK113 pKa = 10.55 DD114 pKa = 3.22 ARR116 pKa = 11.84 LAAVSGDD123 pKa = 3.35 IADD126 pKa = 4.08 VPDD129 pKa = 5.37 DD130 pKa = 3.51 IYY132 pKa = 10.94 IRR134 pKa = 11.84 YY135 pKa = 9.04 YY136 pKa = 9.69 RR137 pKa = 11.84 TLKK140 pKa = 10.48 EE141 pKa = 3.53 ISKK144 pKa = 10.57 DD145 pKa = 3.11 HH146 pKa = 6.28 MARR149 pKa = 11.84 PDD151 pKa = 3.74 GLDD154 pKa = 3.59 GVCGLWLYY162 pKa = 10.93 RR163 pKa = 11.84 SAGTGKK169 pKa = 9.94 SRR171 pKa = 11.84 YY172 pKa = 8.88 AQEE175 pKa = 4.2 QFPEE179 pKa = 4.74 HH180 pKa = 5.99 YY181 pKa = 10.17 MKK183 pKa = 8.5 MTNKK187 pKa = 9.21 WCDD190 pKa = 3.2 GYY192 pKa = 11.06 QGQDD196 pKa = 3.36 VVVMDD201 pKa = 6.11 DD202 pKa = 3.94 MDD204 pKa = 5.44 PDD206 pKa = 4.17 HH207 pKa = 7.17 ACLRR211 pKa = 11.84 HH212 pKa = 5.75 HH213 pKa = 7.46 LKK215 pKa = 10.36 RR216 pKa = 11.84 WADD219 pKa = 3.79 RR220 pKa = 11.84 YY221 pKa = 10.38 PFIGEE226 pKa = 4.28 TKK228 pKa = 10.22 GGAISIRR235 pKa = 11.84 PKK237 pKa = 10.55 KK238 pKa = 10.31 FVVTSQYY245 pKa = 11.01 SIEE248 pKa = 4.84 DD249 pKa = 3.33 MFKK252 pKa = 10.94 NRR254 pKa = 11.84 DD255 pKa = 3.08 GTLDD259 pKa = 3.59 VEE261 pKa = 4.72 TVAAIRR267 pKa = 11.84 RR268 pKa = 11.84 RR269 pKa = 11.84 FEE271 pKa = 3.87 VKK273 pKa = 10.28 RR274 pKa = 11.84 FGVNAFNPYY283 pKa = 10.39 NII285 pKa = 4.5
Molecular weight: 32.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.71
IPC2_protein 6.839
IPC_protein 6.927
Toseland 6.781
ProMoST 7.41
Dawson 7.527
Bjellqvist 7.614
Wikipedia 7.468
Rodwell 7.527
Grimsley 6.854
Solomon 7.585
Lehninger 7.614
Nozaki 7.892
DTASelect 7.717
Thurlkill 7.746
EMBOSS 7.805
Sillero 7.995
Patrickios 4.151
IPC_peptide 7.585
IPC2_peptide 7.249
IPC2.peptide.svr19 7.18
Protein with the highest isoelectric point:
>tr|W5U2P7|W5U2P7_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-3 OX=1454024 PE=4 SV=1
MM1 pKa = 7.2 VLGKK5 pKa = 10.08 RR6 pKa = 11.84 GRR8 pKa = 11.84 PGSMKK13 pKa = 10.27 YY14 pKa = 9.62 VPRR17 pKa = 11.84 YY18 pKa = 9.15 AKK20 pKa = 10.36 RR21 pKa = 11.84 ITRR24 pKa = 11.84 GPYY27 pKa = 8.77 VPRR30 pKa = 11.84 GLTLRR35 pKa = 11.84 PNNASVARR43 pKa = 11.84 QVEE46 pKa = 4.56 MKK48 pKa = 10.39 FVDD51 pKa = 3.79 ISPTFALDD59 pKa = 3.69 FATPYY64 pKa = 10.43 HH65 pKa = 6.63 INLLNGIATGSDD77 pKa = 3.48 LYY79 pKa = 11.14 QRR81 pKa = 11.84 VGRR84 pKa = 11.84 SVYY87 pKa = 9.64 MKK89 pKa = 10.2 NLHH92 pKa = 7.22 IDD94 pKa = 4.17 FQCQNINTGSSTPDD108 pKa = 2.91 LEE110 pKa = 4.18 MRR112 pKa = 11.84 VLVVLDD118 pKa = 3.78 KK119 pKa = 11.27 APNGAALPVLADD131 pKa = 3.9 FLRR134 pKa = 11.84 EE135 pKa = 3.69 INAAGAATTNLNSHH149 pKa = 6.02 QNTNNRR155 pKa = 11.84 QRR157 pKa = 11.84 FKK159 pKa = 11.29 FLFDD163 pKa = 3.62 KK164 pKa = 10.97 KK165 pKa = 10.61 IGIPPWNVGATTTGAPALNMVDD187 pKa = 3.78 VYY189 pKa = 9.49 WRR191 pKa = 11.84 KK192 pKa = 9.81 HH193 pKa = 4.72 IKK195 pKa = 9.01 CTTRR199 pKa = 11.84 CSSLEE204 pKa = 3.93 KK205 pKa = 10.46 GHH207 pKa = 6.85 RR208 pKa = 11.84 CQKK211 pKa = 9.97 SRR213 pKa = 11.84 TMVYY217 pKa = 8.44 TT218 pKa = 3.93
Molecular weight: 24.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.633
IPC_protein 10.028
Toseland 10.365
ProMoST 10.028
Dawson 10.526
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.891
Grimsley 10.584
Solomon 10.57
Lehninger 10.526
Nozaki 10.365
DTASelect 10.189
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.438
Patrickios 10.57
IPC_peptide 10.57
IPC2_peptide 9.136
IPC2.peptide.svr19 8.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
503
218
285
251.5
28.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.344 ± 1.036
2.187 ± 0.236
5.765 ± 1.097
4.175 ± 1.568
3.976 ± 0.205
7.753 ± 0.585
2.584 ± 0.195
4.175 ± 0.276
5.765 ± 0.133
6.561 ± 1.136
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.181 ± 0.02
5.567 ± 1.188
5.169 ± 0.532
2.982 ± 0.153
7.952 ± 0.204
4.573 ± 0.317
5.567 ± 1.495
6.958 ± 0.255
1.392 ± 0.318
4.374 ± 0.472
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here