Arthrobacter phage Jasmine
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U3TK18|A0A0U3TK18_9CAUD Uncharacterized protein OS=Arthrobacter phage Jasmine OX=1772302 GN=51 PE=4 SV=1
MM1 pKa = 7.76 EE2 pKa = 4.71 EE3 pKa = 6.02 FEE5 pKa = 4.48 TAWNIVGIVCSWIIAGILLALLVGAIVAIWNEE37 pKa = 4.14 YY38 pKa = 8.46 FF39 pKa = 3.74
Molecular weight: 4.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.238
IPC2_protein 3.999
IPC_protein 3.287
Toseland 3.35
ProMoST 3.63
Dawson 3.363
Bjellqvist 3.579
Wikipedia 3.363
Rodwell 3.287
Grimsley 3.338
Solomon 3.007
Lehninger 2.956
Nozaki 3.643
DTASelect 3.376
Thurlkill 3.49
EMBOSS 3.376
Sillero 3.478
Patrickios 0.006
IPC_peptide 3.02
IPC2_peptide 3.389
IPC2.peptide.svr19 3.61
Protein with the highest isoelectric point:
>tr|A0A0U4KNQ5|A0A0U4KNQ5_9CAUD Major capsid protein OS=Arthrobacter phage Jasmine OX=1772302 GN=17 PE=4 SV=1
MM1 pKa = 6.44 EE2 pKa = 4.55 QEE4 pKa = 4.22 MFGAPIVRR12 pKa = 11.84 WDD14 pKa = 3.43 SRR16 pKa = 11.84 NPSAYY21 pKa = 10.06 GRR23 pKa = 11.84 MIVAGRR29 pKa = 11.84 RR30 pKa = 11.84 GGKK33 pKa = 6.39 TQRR36 pKa = 11.84 QRR38 pKa = 11.84 EE39 pKa = 4.09 WSSLLFDD46 pKa = 6.16 EE47 pKa = 5.65 ILEE50 pKa = 4.48 TQNLMNKK57 pKa = 9.96 AKK59 pKa = 10.11 VRR61 pKa = 11.84 DD62 pKa = 3.98 YY63 pKa = 11.28 IRR65 pKa = 11.84 TQSPLAEE72 pKa = 4.07 SVRR75 pKa = 11.84 PNYY78 pKa = 9.35 IQSRR82 pKa = 11.84 WVRR85 pKa = 11.84 FANGHH90 pKa = 4.77 GRR92 pKa = 11.84 NNAVVVFSLSLDD104 pKa = 3.63 AQGDD108 pKa = 3.78 RR109 pKa = 11.84 QLIFTMDD116 pKa = 3.47 QINRR120 pKa = 11.84 LSTTRR125 pKa = 11.84 EE126 pKa = 3.62 TIYY129 pKa = 10.69 RR130 pKa = 11.84 LSALSVTIAGTPLMTSFILSLDD152 pKa = 3.2 QLMEE156 pKa = 3.75 KK157 pKa = 10.03 RR158 pKa = 11.84 GYY160 pKa = 8.05 QFRR163 pKa = 11.84 QKK165 pKa = 10.67 VNPYY169 pKa = 9.68 PLWQEE174 pKa = 3.97 FF175 pKa = 3.37
Molecular weight: 20.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 9.575
IPC_protein 10.409
Toseland 10.233
ProMoST 10.058
Dawson 10.452
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.555
Grimsley 10.54
Solomon 10.526
Lehninger 10.467
Nozaki 10.189
DTASelect 10.189
Thurlkill 10.292
EMBOSS 10.643
Sillero 10.365
Patrickios 10.145
IPC_peptide 10.511
IPC2_peptide 9.033
IPC2.peptide.svr19 8.657
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
14347
34
1803
256.2
28.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.713 ± 0.434
0.599 ± 0.099
6.064 ± 0.183
6.838 ± 0.439
3.987 ± 0.235
7.444 ± 0.346
1.84 ± 0.122
5.562 ± 0.186
6.322 ± 0.408
7.256 ± 0.256
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.774 ± 0.18
5.395 ± 0.252
4.363 ± 0.243
4.301 ± 0.369
4.712 ± 0.224
6.259 ± 0.277
6.357 ± 0.357
6.357 ± 0.273
1.554 ± 0.146
3.304 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here