Streptococcus phage Javan105

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZXF4|A0A4D5ZXF4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan105 OX=2547977 GN=Javan105_0005 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 5.45KK3 pKa = 10.69LLEE6 pKa = 4.69IIEE9 pKa = 4.72KK10 pKa = 10.26IGFPSAYY17 pKa = 9.15HH18 pKa = 6.19HH19 pKa = 6.06FAEE22 pKa = 5.44GEE24 pKa = 4.4SPNPPFLIYY33 pKa = 10.31ILPASDD39 pKa = 3.92NFSADD44 pKa = 2.46GRR46 pKa = 11.84VYY48 pKa = 9.99FKK50 pKa = 11.14ANEE53 pKa = 3.89VHH55 pKa = 6.62IEE57 pKa = 3.99VYY59 pKa = 10.1TDD61 pKa = 3.34YY62 pKa = 11.6KK63 pKa = 11.26NLDD66 pKa = 3.22IEE68 pKa = 4.55KK69 pKa = 9.87KK70 pKa = 10.05VEE72 pKa = 4.13VVLDD76 pKa = 4.03EE77 pKa = 4.57YY78 pKa = 11.46GIFYY82 pKa = 10.57NKK84 pKa = 10.27SEE86 pKa = 4.22VYY88 pKa = 10.04IEE90 pKa = 4.13SEE92 pKa = 3.7KK93 pKa = 10.87LYY95 pKa = 9.42EE96 pKa = 3.81VLYY99 pKa = 10.08IFEE102 pKa = 4.88MEE104 pKa = 4.16VKK106 pKa = 9.97TNGKK110 pKa = 9.41

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZXF8|A0A4D5ZXF8_9CAUD Cytosine-specific methyltransferase OS=Streptococcus phage Javan105 OX=2547977 GN=Javan105_0033 PE=3 SV=1
MM1 pKa = 7.64VSLKK5 pKa = 9.96QKK7 pKa = 10.35KK8 pKa = 9.18IRR10 pKa = 11.84EE11 pKa = 3.38IWAFVNFAGSQFLSLLEE28 pKa = 3.95EE29 pKa = 4.15RR30 pKa = 11.84KK31 pKa = 9.84KK32 pKa = 10.99DD33 pKa = 3.42SAQIAVEE40 pKa = 4.3TNGGIAIWMRR50 pKa = 3.49

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

16610

38

937

255.5

29.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.201 ± 0.334

1.162 ± 0.106

6.177 ± 0.199

8.17 ± 0.318

3.992 ± 0.17

6.261 ± 0.288

1.583 ± 0.109

8.019 ± 0.246

8.94 ± 0.321

8.338 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.14

5.527 ± 0.214

2.974 ± 0.192

2.95 ± 0.157

4.238 ± 0.26

6.526 ± 0.287

5.172 ± 0.2

5.822 ± 0.18

1.168 ± 0.09

4.148 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski