Klebsiella phage ST512-KPC3phi13.3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MGU4|A0A482MGU4_9CAUD Uncharacterized protein OS=Klebsiella phage ST512-KPC3phi13.3 OX=2510471 PE=4 SV=1
MM1 pKa = 7.34NKK3 pKa = 9.26VEE5 pKa = 4.37LLEE8 pKa = 4.98KK9 pKa = 10.4ISALATEE16 pKa = 4.81CHH18 pKa = 5.91TLACEE23 pKa = 4.54LDD25 pKa = 3.42IGDD28 pKa = 4.53EE29 pKa = 4.01RR30 pKa = 11.84TEE32 pKa = 3.78MFEE35 pKa = 4.27IYY37 pKa = 10.23SVLHH41 pKa = 4.75NLCRR45 pKa = 11.84RR46 pKa = 11.84GYY48 pKa = 9.06ASQVGRR54 pKa = 11.84RR55 pKa = 11.84MNPLLASCDD64 pKa = 3.78DD65 pKa = 4.2EE66 pKa = 4.88EE67 pKa = 6.51DD68 pKa = 4.33EE69 pKa = 6.27EE70 pKa = 6.66GDD72 pKa = 5.29DD73 pKa = 4.65WDD75 pKa = 4.93EE76 pKa = 5.93DD77 pKa = 4.05DD78 pKa = 5.75DD79 pKa = 4.45

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MF53|A0A482MF53_9CAUD Uncharacterized protein OS=Klebsiella phage ST512-KPC3phi13.3 OX=2510471 PE=4 SV=1
MM1 pKa = 7.51LPSQSPAIFTVSRR14 pKa = 11.84LNQTVRR20 pKa = 11.84LLLEE24 pKa = 4.18RR25 pKa = 11.84EE26 pKa = 4.17MGQVWISGEE35 pKa = 3.75ISNFSQPSSGHH46 pKa = 5.4WYY48 pKa = 7.89FTLKK52 pKa = 10.65DD53 pKa = 3.48DD54 pKa = 4.28NAQVRR59 pKa = 11.84CAMFRR64 pKa = 11.84NSNRR68 pKa = 11.84RR69 pKa = 11.84VTFRR73 pKa = 11.84PQHH76 pKa = 4.89GQQVLVRR83 pKa = 11.84ANITLYY89 pKa = 10.41EE90 pKa = 3.95PRR92 pKa = 11.84GDD94 pKa = 3.52YY95 pKa = 10.62QIIVEE100 pKa = 4.24SMQPAGEE107 pKa = 4.28GLLQQKK113 pKa = 9.56YY114 pKa = 7.98EE115 pKa = 3.96QLKK118 pKa = 10.07AQLTAEE124 pKa = 4.22GLFEE128 pKa = 4.22QKK130 pKa = 10.06HH131 pKa = 4.87KK132 pKa = 10.76QALPSPAHH140 pKa = 5.81CVGVITSKK148 pKa = 9.77TGAALHH154 pKa = 7.17DD155 pKa = 3.88ILHH158 pKa = 5.44VLRR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84DD164 pKa = 3.27PGLPVIIYY172 pKa = 6.6PTAVQGDD179 pKa = 4.28DD180 pKa = 3.52APGQIVRR187 pKa = 11.84AIALANARR195 pKa = 11.84QEE197 pKa = 4.1CDD199 pKa = 3.23VLIVGRR205 pKa = 11.84GGGSLEE211 pKa = 4.7DD212 pKa = 2.99LWSFNDD218 pKa = 3.26EE219 pKa = 3.92RR220 pKa = 11.84VARR223 pKa = 11.84AIFASQIPIVSAVGHH238 pKa = 5.46EE239 pKa = 4.25TDD241 pKa = 3.12VTIADD246 pKa = 4.4FVADD250 pKa = 4.65LRR252 pKa = 11.84APTPSAAAEE261 pKa = 3.88IVSRR265 pKa = 11.84NQQEE269 pKa = 4.85LLRR272 pKa = 11.84QLQSGQQRR280 pKa = 11.84LEE282 pKa = 3.56MAMDD286 pKa = 3.74YY287 pKa = 10.8FLASRR292 pKa = 11.84QRR294 pKa = 11.84RR295 pKa = 11.84FTQLFHH301 pKa = 7.77RR302 pKa = 11.84LQQQHH307 pKa = 5.81PQLRR311 pKa = 11.84LARR314 pKa = 11.84QQTALEE320 pKa = 4.14RR321 pKa = 11.84LRR323 pKa = 11.84QRR325 pKa = 11.84MRR327 pKa = 11.84IAVEE331 pKa = 4.13SQLKK335 pKa = 10.03RR336 pKa = 11.84AEE338 pKa = 3.99QRR340 pKa = 11.84QKK342 pKa = 10.32RR343 pKa = 11.84TVLRR347 pKa = 11.84LNHH350 pKa = 6.2YY351 pKa = 9.3NPQPRR356 pKa = 11.84IHH358 pKa = 7.04RR359 pKa = 11.84AQSRR363 pKa = 11.84IQQLEE368 pKa = 3.89YY369 pKa = 10.68RR370 pKa = 11.84LAEE373 pKa = 3.93IMRR376 pKa = 11.84GRR378 pKa = 11.84LSEE381 pKa = 3.84RR382 pKa = 11.84RR383 pKa = 11.84EE384 pKa = 3.89RR385 pKa = 11.84FGNAVTHH392 pKa = 6.54LEE394 pKa = 4.14AVSPLATLARR404 pKa = 11.84GYY406 pKa = 10.75SVTSVSDD413 pKa = 3.45GTVLKK418 pKa = 8.82QTKK421 pKa = 7.31QVKK424 pKa = 9.16TGDD427 pKa = 3.72LLTTRR432 pKa = 11.84LKK434 pKa = 11.04DD435 pKa = 3.13GWVEE439 pKa = 4.28SEE441 pKa = 4.33VKK443 pKa = 10.6QIATVKK449 pKa = 8.96KK450 pKa = 7.55TRR452 pKa = 11.84ARR454 pKa = 11.84KK455 pKa = 8.91PSPTKK460 pKa = 9.9PAEE463 pKa = 3.85

Molecular weight: 52.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

18932

44

1129

252.4

27.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.983 ± 0.431

1.093 ± 0.117

5.895 ± 0.2

6.117 ± 0.324

3.365 ± 0.163

7.548 ± 0.239

1.891 ± 0.152

5.161 ± 0.217

4.675 ± 0.215

8.758 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.889 ± 0.151

4.083 ± 0.267

4.474 ± 0.252

4.881 ± 0.325

6.407 ± 0.303

6.064 ± 0.265

5.441 ± 0.247

6.75 ± 0.267

1.632 ± 0.146

2.895 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski