Halapricum sp. CBA1109
Average proteome isoelectric point is 4.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2712 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A9SZF5|A0A6A9SZF5_9EURY Uncharacterized protein OS=Halapricum sp. CBA1109 OX=2668068 GN=GJ629_09200 PE=4 SV=1
MM1 pKa = 7.58 VDD3 pKa = 4.92 SRR5 pKa = 11.84 WDD7 pKa = 3.53 DD8 pKa = 3.14 QATYY12 pKa = 10.98 YY13 pKa = 9.52 NQTMPRR19 pKa = 11.84 NCPPVTEE26 pKa = 5.61 DD27 pKa = 3.88 DD28 pKa = 3.81 GTMDD32 pKa = 4.19 GYY34 pKa = 10.83 RR35 pKa = 11.84 VEE37 pKa = 4.3 GAIAGALYY45 pKa = 10.56 DD46 pKa = 4.74 VVDD49 pKa = 4.29 DD50 pKa = 4.83 TPNEE54 pKa = 4.02 DD55 pKa = 3.53 PMNEE59 pKa = 3.97 SFATVYY65 pKa = 10.75 SAFDD69 pKa = 3.32 EE70 pKa = 4.46 EE71 pKa = 4.88 MPQSAFEE78 pKa = 4.47 VYY80 pKa = 10.09 DD81 pKa = 3.8 ALAEE85 pKa = 4.11 RR86 pKa = 11.84 VDD88 pKa = 4.33 NEE90 pKa = 4.08 TALQQVFTTHH100 pKa = 7.19 GIDD103 pKa = 3.38 VAEE106 pKa = 4.66 PALTVDD112 pKa = 4.36 GGPNHH117 pKa = 6.68 SSYY120 pKa = 11.77 GNEE123 pKa = 3.81 TTITGTAVDD132 pKa = 3.73 EE133 pKa = 4.59 HH134 pKa = 8.47 SNITRR139 pKa = 11.84 VQASVDD145 pKa = 3.43 GGPWSTVAGDD155 pKa = 5.41 DD156 pKa = 3.5 ATEE159 pKa = 3.88 WSVTVEE165 pKa = 4.29 GPTDD169 pKa = 3.49 TVTIRR174 pKa = 11.84 ATDD177 pKa = 2.95 HH178 pKa = 6.34 WGNNATRR185 pKa = 11.84 NVTVEE190 pKa = 3.88 QSAVPSVVGGRR201 pKa = 11.84 PITDD205 pKa = 3.54 PDD207 pKa = 3.82 GDD209 pKa = 4.02 GVYY212 pKa = 10.74 EE213 pKa = 4.54 DD214 pKa = 4.02 VDD216 pKa = 4.0 GDD218 pKa = 4.16 GEE220 pKa = 4.88 ADD222 pKa = 3.71 LFDD225 pKa = 4.62 ALVYY229 pKa = 10.48 YY230 pKa = 10.43 NNRR233 pKa = 11.84 NSGVIEE239 pKa = 3.83 AHH241 pKa = 6.76 PGLFDD246 pKa = 3.99 YY247 pKa = 11.0 DD248 pKa = 4.14 SEE250 pKa = 5.82 DD251 pKa = 4.14 DD252 pKa = 3.84 VGTLFDD258 pKa = 4.31 ALALFEE264 pKa = 5.14 MIGAPNRR271 pKa = 3.66
Molecular weight: 29.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.592
IPC_protein 3.63
Toseland 3.401
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.567
Rodwell 3.452
Grimsley 3.3
Solomon 3.617
Lehninger 3.567
Nozaki 3.732
DTASelect 3.986
Thurlkill 3.452
EMBOSS 3.579
Sillero 3.745
Patrickios 1.176
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.693
Protein with the highest isoelectric point:
>tr|A0A6A9T8L7|A0A6A9T8L7_9EURY Uncharacterized protein OS=Halapricum sp. CBA1109 OX=2668068 GN=GJ629_05530 PE=4 SV=1
MM1 pKa = 7.28 VVLLVVASAVPLARR15 pKa = 11.84 PSPRR19 pKa = 11.84 RR20 pKa = 11.84 TAAVVTRR27 pKa = 11.84 RR28 pKa = 11.84 HH29 pKa = 4.96 QPRR32 pKa = 11.84 TTGRR36 pKa = 11.84 RR37 pKa = 11.84 HH38 pKa = 5.75 PTTGPEE44 pKa = 3.46 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 PDD49 pKa = 2.86 RR50 pKa = 11.84 RR51 pKa = 11.84 TRR53 pKa = 11.84 RR54 pKa = 3.44
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.383
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.842
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2712
0
2712
727707
31
1997
268.3
29.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.874 ± 0.063
0.688 ± 0.013
8.704 ± 0.065
8.032 ± 0.071
3.216 ± 0.035
8.696 ± 0.05
1.882 ± 0.027
3.871 ± 0.037
1.635 ± 0.03
8.653 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.712 ± 0.022
2.243 ± 0.03
4.771 ± 0.038
2.528 ± 0.031
6.686 ± 0.049
5.79 ± 0.04
6.878 ± 0.052
9.276 ± 0.058
1.111 ± 0.019
2.755 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here