Halapricum sp. CBA1109

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Halapricum; unclassified Halapricum

Average proteome isoelectric point is 4.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6A9SZF5|A0A6A9SZF5_9EURY Uncharacterized protein OS=Halapricum sp. CBA1109 OX=2668068 GN=GJ629_09200 PE=4 SV=1
MM1 pKa = 7.58VDD3 pKa = 4.92SRR5 pKa = 11.84WDD7 pKa = 3.53DD8 pKa = 3.14QATYY12 pKa = 10.98YY13 pKa = 9.52NQTMPRR19 pKa = 11.84NCPPVTEE26 pKa = 5.61DD27 pKa = 3.88DD28 pKa = 3.81GTMDD32 pKa = 4.19GYY34 pKa = 10.83RR35 pKa = 11.84VEE37 pKa = 4.3GAIAGALYY45 pKa = 10.56DD46 pKa = 4.74VVDD49 pKa = 4.29DD50 pKa = 4.83TPNEE54 pKa = 4.02DD55 pKa = 3.53PMNEE59 pKa = 3.97SFATVYY65 pKa = 10.75SAFDD69 pKa = 3.32EE70 pKa = 4.46EE71 pKa = 4.88MPQSAFEE78 pKa = 4.47VYY80 pKa = 10.09DD81 pKa = 3.8ALAEE85 pKa = 4.11RR86 pKa = 11.84VDD88 pKa = 4.33NEE90 pKa = 4.08TALQQVFTTHH100 pKa = 7.19GIDD103 pKa = 3.38VAEE106 pKa = 4.66PALTVDD112 pKa = 4.36GGPNHH117 pKa = 6.68SSYY120 pKa = 11.77GNEE123 pKa = 3.81TTITGTAVDD132 pKa = 3.73EE133 pKa = 4.59HH134 pKa = 8.47SNITRR139 pKa = 11.84VQASVDD145 pKa = 3.43GGPWSTVAGDD155 pKa = 5.41DD156 pKa = 3.5ATEE159 pKa = 3.88WSVTVEE165 pKa = 4.29GPTDD169 pKa = 3.49TVTIRR174 pKa = 11.84ATDD177 pKa = 2.95HH178 pKa = 6.34WGNNATRR185 pKa = 11.84NVTVEE190 pKa = 3.88QSAVPSVVGGRR201 pKa = 11.84PITDD205 pKa = 3.54PDD207 pKa = 3.82GDD209 pKa = 4.02GVYY212 pKa = 10.74EE213 pKa = 4.54DD214 pKa = 4.02VDD216 pKa = 4.0GDD218 pKa = 4.16GEE220 pKa = 4.88ADD222 pKa = 3.71LFDD225 pKa = 4.62ALVYY229 pKa = 10.48YY230 pKa = 10.43NNRR233 pKa = 11.84NSGVIEE239 pKa = 3.83AHH241 pKa = 6.76PGLFDD246 pKa = 3.99YY247 pKa = 11.0DD248 pKa = 4.14SEE250 pKa = 5.82DD251 pKa = 4.14DD252 pKa = 3.84VGTLFDD258 pKa = 4.31ALALFEE264 pKa = 5.14MIGAPNRR271 pKa = 3.66

Molecular weight:
29.24 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6A9T8L7|A0A6A9T8L7_9EURY Uncharacterized protein OS=Halapricum sp. CBA1109 OX=2668068 GN=GJ629_05530 PE=4 SV=1
MM1 pKa = 7.28VVLLVVASAVPLARR15 pKa = 11.84PSPRR19 pKa = 11.84RR20 pKa = 11.84TAAVVTRR27 pKa = 11.84RR28 pKa = 11.84HH29 pKa = 4.96QPRR32 pKa = 11.84TTGRR36 pKa = 11.84RR37 pKa = 11.84HH38 pKa = 5.75PTTGPEE44 pKa = 3.46RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84PDD49 pKa = 2.86RR50 pKa = 11.84RR51 pKa = 11.84TRR53 pKa = 11.84RR54 pKa = 3.44

Molecular weight:
6.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2712

0

2712

727707

31

1997

268.3

29.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.874 ± 0.063

0.688 ± 0.013

8.704 ± 0.065

8.032 ± 0.071

3.216 ± 0.035

8.696 ± 0.05

1.882 ± 0.027

3.871 ± 0.037

1.635 ± 0.03

8.653 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.712 ± 0.022

2.243 ± 0.03

4.771 ± 0.038

2.528 ± 0.031

6.686 ± 0.049

5.79 ± 0.04

6.878 ± 0.052

9.276 ± 0.058

1.111 ± 0.019

2.755 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski