Eubacterium infirmum F0142

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; [Eubacterium] infirmum

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1PLJ3|H1PLJ3_9FIRM GTPase Obg OS=Eubacterium infirmum F0142 OX=883109 GN=obg PE=3 SV=1
MM1 pKa = 6.12MTKK4 pKa = 10.36EE5 pKa = 4.06EE6 pKa = 4.39LFEE9 pKa = 4.34LVDD12 pKa = 3.48IEE14 pKa = 4.31TGEE17 pKa = 4.11DD18 pKa = 3.06FTYY21 pKa = 10.97FEE23 pKa = 4.35NFANLMEE30 pKa = 5.54ADD32 pKa = 3.92EE33 pKa = 5.21YY34 pKa = 10.43ITEE37 pKa = 4.21EE38 pKa = 4.9DD39 pKa = 2.71IGMLIKK45 pKa = 10.6EE46 pKa = 4.69LDD48 pKa = 3.66CVTFSEE54 pKa = 4.42LAEE57 pKa = 4.31SYY59 pKa = 10.33FYY61 pKa = 11.34DD62 pKa = 4.26VMEE65 pKa = 5.14HH66 pKa = 6.6LPDD69 pKa = 3.7NAIDD73 pKa = 3.66IYY75 pKa = 11.21NTMEE79 pKa = 3.84AVKK82 pKa = 10.34RR83 pKa = 11.84NIVSISTAIAKK94 pKa = 10.4GEE96 pKa = 4.09EE97 pKa = 4.26QSHH100 pKa = 5.48KK101 pKa = 10.56LCRR104 pKa = 11.84EE105 pKa = 4.34LYY107 pKa = 10.44NFRR110 pKa = 11.84NWYY113 pKa = 9.4IDD115 pKa = 3.54PQSCFATDD123 pKa = 3.64LTSGNEE129 pKa = 4.09DD130 pKa = 3.22VMSIRR135 pKa = 11.84DD136 pKa = 3.84AIYY139 pKa = 9.19EE140 pKa = 4.1NKK142 pKa = 10.26LAGITKK148 pKa = 9.5TDD150 pKa = 2.96WNFDD154 pKa = 3.45FSEE157 pKa = 4.51CNQLEE162 pKa = 3.85ISEE165 pKa = 4.39YY166 pKa = 9.94IINIGEE172 pKa = 4.07LSS174 pKa = 3.28

Molecular weight: 20.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1PJE2|H1PJE2_9FIRM R3H domain-containing protein OS=Eubacterium infirmum F0142 OX=883109 GN=HMPREF0380_00300 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 9.53QTFQPKK8 pKa = 8.61NRR10 pKa = 11.84QRR12 pKa = 11.84MKK14 pKa = 9.6EE15 pKa = 3.53HH16 pKa = 6.78GFRR19 pKa = 11.84KK20 pKa = 10.05RR21 pKa = 11.84MATKK25 pKa = 10.39NGRR28 pKa = 11.84NVLKK32 pKa = 10.54RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1765

0

1765

572512

29

2947

324.4

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.092 ± 0.057

1.319 ± 0.031

6.053 ± 0.049

7.405 ± 0.073

4.278 ± 0.046

6.942 ± 0.053

1.552 ± 0.023

8.432 ± 0.06

8.159 ± 0.061

8.793 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.889 ± 0.031

4.86 ± 0.04

3.042 ± 0.028

2.395 ± 0.029

4.212 ± 0.05

6.452 ± 0.044

4.933 ± 0.038

6.622 ± 0.047

0.725 ± 0.014

3.844 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski