Corynebacterium atypicum
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1533 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075TTJ3|A0A075TTJ3_9CORY Aspartate-semialdehyde dehydrogenase OS=Corynebacterium atypicum OX=191610 GN=asd PE=3 SV=1
MM1 pKa = 7.78 KK2 pKa = 8.83 MTDD5 pKa = 4.34 DD6 pKa = 4.92 DD7 pKa = 4.78 GEE9 pKa = 4.6 LPVFLDD15 pKa = 3.54 DD16 pKa = 4.41 QSYY19 pKa = 7.81 RR20 pKa = 11.84 TCSEE24 pKa = 4.16 CTSDD28 pKa = 4.76 CVPDD32 pKa = 3.88 PFIAGEE38 pKa = 4.18 GQGIRR43 pKa = 11.84 IAFICPSCGVQSVIDD58 pKa = 4.01 PFEE61 pKa = 4.15 DD62 pKa = 3.4 TRR64 pKa = 4.57
Molecular weight: 7.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.745
IPC_protein 3.681
Toseland 3.465
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.617
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.808
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A075TTS0|A0A075TTS0_9CORY Delta-aminolevulinic acid dehydratase OS=Corynebacterium atypicum OX=191610 GN=CATYP_01650 PE=3 SV=1
MM1 pKa = 7.47 SKK3 pKa = 10.58 GKK5 pKa = 8.48 RR6 pKa = 11.84 TYY8 pKa = 10.09 QPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.01 HH19 pKa = 4.51 GFRR22 pKa = 11.84 IRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIIAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.72 GRR42 pKa = 11.84 AKK44 pKa = 9.63 LTAA47 pKa = 4.21
Molecular weight: 5.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.213
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.427
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.113
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1533
0
1533
491884
40
1636
320.9
34.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.883 ± 0.097
0.759 ± 0.016
5.972 ± 0.054
6.494 ± 0.066
3.229 ± 0.038
8.753 ± 0.056
2.131 ± 0.026
4.525 ± 0.047
3.064 ± 0.047
9.406 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.014 ± 0.029
2.448 ± 0.03
5.18 ± 0.05
3.404 ± 0.034
6.873 ± 0.062
5.357 ± 0.035
5.665 ± 0.042
8.408 ± 0.061
1.323 ± 0.025
2.112 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here