Pseudoalteromonas phage Maelstrom
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5BG46|A0A2H5BG46_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage Maelstrom OX=2065202 GN=MAELSTROM_51 PE=4 SV=1
MM1 pKa = 7.54 EE2 pKa = 6.15 SISIDD7 pKa = 4.19 AIPSQQFNISLGGNNYY23 pKa = 9.56 KK24 pKa = 10.24 IKK26 pKa = 10.16 IYY28 pKa = 10.64 SIDD31 pKa = 2.93 GHH33 pKa = 5.65 MSYY36 pKa = 11.0 DD37 pKa = 3.6 LSINSVGVISGFKK50 pKa = 10.16 LVNDD54 pKa = 4.05 VPLLPYY60 pKa = 10.08 KK61 pKa = 10.22 YY62 pKa = 10.32 QEE64 pKa = 3.86 INGNIILSLPEE75 pKa = 4.96 DD76 pKa = 4.75 EE77 pKa = 4.82 IPDD80 pKa = 3.69 YY81 pKa = 11.4 EE82 pKa = 4.76 RR83 pKa = 11.84 FGLSQFLFYY92 pKa = 10.81 LDD94 pKa = 3.63 EE95 pKa = 6.25 DD96 pKa = 3.69 EE97 pKa = 4.66 TIEE100 pKa = 4.08 YY101 pKa = 10.49 RR102 pKa = 11.84 EE103 pKa = 4.06 AANLL107 pKa = 3.5
Molecular weight: 12.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.837
IPC2_protein 4.088
IPC_protein 3.999
Toseland 3.808
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.719
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.253
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.113
Patrickios 0.896
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.002
Protein with the highest isoelectric point:
>tr|A0A2H5BG62|A0A2H5BG62_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage Maelstrom OX=2065202 GN=MAELSTROM_38 PE=4 SV=1
MM1 pKa = 7.5 GKK3 pKa = 9.6 IKK5 pKa = 10.66 LYY7 pKa = 10.59 RR8 pKa = 11.84 NAEE11 pKa = 3.55 ARR13 pKa = 11.84 YY14 pKa = 9.27 CVVNSSGKK22 pKa = 10.43 SIGIKK27 pKa = 10.39 VKK29 pKa = 10.08 IKK31 pKa = 8.72 TKK33 pKa = 10.02 RR34 pKa = 11.84 GVRR37 pKa = 11.84 RR38 pKa = 11.84 IRR40 pKa = 11.84 NQRR43 pKa = 11.84 VISRR47 pKa = 11.84 RR48 pKa = 11.84 FVATNYY54 pKa = 9.89 NGCSYY59 pKa = 9.2 ITAGKK64 pKa = 9.53 HH65 pKa = 5.1 YY66 pKa = 10.11 PFTLYY71 pKa = 10.88 GEE73 pKa = 4.42 RR74 pKa = 11.84 KK75 pKa = 8.25 EE76 pKa = 3.94 GRR78 pKa = 11.84 PVFFHH83 pKa = 6.44 TICNRR88 pKa = 11.84 GVRR91 pKa = 11.84 LVAKK95 pKa = 8.55 TKK97 pKa = 10.43 NCPHH101 pKa = 7.0 LSGNNWQWGVII112 pKa = 3.81
Molecular weight: 12.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 10.16
IPC_protein 10.994
Toseland 11.067
ProMoST 10.891
Dawson 11.14
Bjellqvist 10.906
Wikipedia 11.389
Rodwell 11.374
Grimsley 11.184
Solomon 11.316
Lehninger 11.272
Nozaki 11.052
DTASelect 10.891
Thurlkill 11.052
EMBOSS 11.477
Sillero 11.082
Patrickios 11.096
IPC_peptide 11.33
IPC2_peptide 10.087
IPC2.peptide.svr19 8.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
14037
35
909
209.5
23.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.691 ± 0.426
1.097 ± 0.172
6.568 ± 0.221
6.889 ± 0.369
4.082 ± 0.251
7.217 ± 0.336
1.418 ± 0.171
6.711 ± 0.273
7.074 ± 0.499
7.138 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.465 ± 0.154
5.329 ± 0.29
3.227 ± 0.181
3.534 ± 0.154
4.146 ± 0.197
7.373 ± 0.319
5.984 ± 0.335
6.583 ± 0.273
1.104 ± 0.099
3.37 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here