Amino acid dipepetide frequency for Pseudoalteromonas phage Maelstrom

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.834AlaAla: 8.834 ± 1.037
0.926AlaCys: 0.926 ± 0.25
5.058AlaAsp: 5.058 ± 0.674
5.7AlaGlu: 5.7 ± 1.011
3.705AlaPhe: 3.705 ± 0.411
5.913AlaGly: 5.913 ± 0.802
1.639AlaHis: 1.639 ± 0.482
5.913AlaIle: 5.913 ± 0.795
5.628AlaLys: 5.628 ± 0.795
6.768AlaLeu: 6.768 ± 0.846
2.992AlaMet: 2.992 ± 0.484
4.132AlaAsn: 4.132 ± 0.543
2.066AlaPro: 2.066 ± 0.423
2.992AlaGln: 2.992 ± 0.563
4.061AlaArg: 4.061 ± 0.533
6.127AlaSer: 6.127 ± 0.745
5.913AlaThr: 5.913 ± 0.829
5.343AlaVal: 5.343 ± 0.671
0.784AlaTrp: 0.784 ± 0.244
2.422AlaTyr: 2.422 ± 0.394
0.0AlaXaa: 0.0 ± 0.0
Cys
0.641CysAla: 0.641 ± 0.225
0.142CysCys: 0.142 ± 0.104
0.855CysAsp: 0.855 ± 0.26
0.926CysGlu: 0.926 ± 0.246
0.499CysPhe: 0.499 ± 0.236
0.641CysGly: 0.641 ± 0.242
0.142CysHis: 0.142 ± 0.091
0.712CysIle: 0.712 ± 0.24
0.57CysLys: 0.57 ± 0.195
0.784CysLeu: 0.784 ± 0.255
0.142CysMet: 0.142 ± 0.097
0.641CysAsn: 0.641 ± 0.183
0.641CysPro: 0.641 ± 0.233
0.214CysGln: 0.214 ± 0.12
0.499CysArg: 0.499 ± 0.223
1.069CysSer: 1.069 ± 0.279
0.214CysThr: 0.214 ± 0.107
0.926CysVal: 0.926 ± 0.219
0.214CysTrp: 0.214 ± 0.124
0.499CysTyr: 0.499 ± 0.22
0.0CysXaa: 0.0 ± 0.0
Asp
5.7AspAla: 5.7 ± 0.748
0.855AspCys: 0.855 ± 0.308
4.488AspAsp: 4.488 ± 0.586
4.203AspGlu: 4.203 ± 0.727
2.422AspPhe: 2.422 ± 0.372
5.486AspGly: 5.486 ± 0.642
1.069AspHis: 1.069 ± 0.343
5.913AspIle: 5.913 ± 0.659
5.058AspLys: 5.058 ± 0.622
4.203AspLeu: 4.203 ± 0.599
1.781AspMet: 1.781 ± 0.394
3.562AspAsn: 3.562 ± 0.619
2.85AspPro: 2.85 ± 0.594
1.639AspGln: 1.639 ± 0.356
1.781AspArg: 1.781 ± 0.335
4.346AspSer: 4.346 ± 0.682
3.064AspThr: 3.064 ± 0.443
3.99AspVal: 3.99 ± 0.401
0.641AspTrp: 0.641 ± 0.258
2.636AspTyr: 2.636 ± 0.462
0.0AspXaa: 0.0 ± 0.0
Glu
5.628GluAla: 5.628 ± 1.046
1.496GluCys: 1.496 ± 0.409
3.491GluAsp: 3.491 ± 0.709
4.417GluGlu: 4.417 ± 0.755
2.992GluPhe: 2.992 ± 0.415
3.349GluGly: 3.349 ± 0.428
0.855GluHis: 0.855 ± 0.241
5.058GluIle: 5.058 ± 0.683
5.272GluLys: 5.272 ± 0.752
7.766GluLeu: 7.766 ± 0.868
1.781GluMet: 1.781 ± 0.334
3.064GluAsn: 3.064 ± 0.438
1.995GluPro: 1.995 ± 0.309
2.066GluGln: 2.066 ± 0.466
3.349GluArg: 3.349 ± 0.415
5.415GluSer: 5.415 ± 0.691
3.42GluThr: 3.42 ± 0.462
4.488GluVal: 4.488 ± 0.581
1.282GluTrp: 1.282 ± 0.298
1.71GluTyr: 1.71 ± 0.278
0.0GluXaa: 0.0 ± 0.0
Phe
2.85PheAla: 2.85 ± 0.463
0.214PheCys: 0.214 ± 0.148
3.206PheAsp: 3.206 ± 0.452
2.351PheGlu: 2.351 ± 0.471
1.425PhePhe: 1.425 ± 0.353
4.061PheGly: 4.061 ± 0.563
0.57PheHis: 0.57 ± 0.208
2.209PheIle: 2.209 ± 0.413
3.064PheLys: 3.064 ± 0.542
2.351PheLeu: 2.351 ± 0.44
0.855PheMet: 0.855 ± 0.264
2.992PheAsn: 2.992 ± 0.458
1.354PhePro: 1.354 ± 0.28
1.211PheGln: 1.211 ± 0.257
1.567PheArg: 1.567 ± 0.306
3.42PheSer: 3.42 ± 0.515
2.494PheThr: 2.494 ± 0.419
2.422PheVal: 2.422 ± 0.493
0.356PheTrp: 0.356 ± 0.176
1.852PheTyr: 1.852 ± 0.398
0.0PheXaa: 0.0 ± 0.0
Gly
5.272GlyAla: 5.272 ± 0.713
0.784GlyCys: 0.784 ± 0.234
4.56GlyAsp: 4.56 ± 0.568
6.198GlyGlu: 6.198 ± 0.677
3.776GlyPhe: 3.776 ± 0.533
5.628GlyGly: 5.628 ± 0.738
1.14GlyHis: 1.14 ± 0.334
4.845GlyIle: 4.845 ± 0.537
5.343GlyLys: 5.343 ± 0.658
4.631GlyLeu: 4.631 ± 0.542
1.425GlyMet: 1.425 ± 0.28
2.707GlyAsn: 2.707 ± 0.441
1.211GlyPro: 1.211 ± 0.266
2.066GlyGln: 2.066 ± 0.56
2.494GlyArg: 2.494 ± 0.403
5.272GlySer: 5.272 ± 0.843
4.346GlyThr: 4.346 ± 0.714
7.338GlyVal: 7.338 ± 0.804
0.641GlyTrp: 0.641 ± 0.19
2.494GlyTyr: 2.494 ± 0.386
0.0GlyXaa: 0.0 ± 0.0
His
1.069HisAla: 1.069 ± 0.249
0.285HisCys: 0.285 ± 0.154
0.997HisAsp: 0.997 ± 0.309
1.14HisGlu: 1.14 ± 0.324
0.499HisPhe: 0.499 ± 0.187
1.211HisGly: 1.211 ± 0.289
0.285HisHis: 0.285 ± 0.123
0.855HisIle: 0.855 ± 0.269
1.496HisLys: 1.496 ± 0.363
1.069HisLeu: 1.069 ± 0.23
0.214HisMet: 0.214 ± 0.11
0.57HisAsn: 0.57 ± 0.211
0.356HisPro: 0.356 ± 0.17
0.356HisGln: 0.356 ± 0.163
0.712HisArg: 0.712 ± 0.208
0.855HisSer: 0.855 ± 0.214
0.712HisThr: 0.712 ± 0.227
0.855HisVal: 0.855 ± 0.284
0.071HisTrp: 0.071 ± 0.071
0.57HisTyr: 0.57 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
5.771IleAla: 5.771 ± 0.732
0.641IleCys: 0.641 ± 0.214
4.987IleAsp: 4.987 ± 0.599
6.27IleGlu: 6.27 ± 0.667
1.71IlePhe: 1.71 ± 0.342
4.346IleGly: 4.346 ± 0.615
0.499IleHis: 0.499 ± 0.198
3.847IleIle: 3.847 ± 0.587
5.058IleLys: 5.058 ± 0.623
3.562IleLeu: 3.562 ± 0.536
1.567IleMet: 1.567 ± 0.375
4.203IleAsn: 4.203 ± 0.571
2.422IlePro: 2.422 ± 0.403
2.351IleGln: 2.351 ± 0.39
2.494IleArg: 2.494 ± 0.423
6.056IleSer: 6.056 ± 0.731
5.272IleThr: 5.272 ± 0.518
3.705IleVal: 3.705 ± 0.44
0.427IleTrp: 0.427 ± 0.159
1.924IleTyr: 1.924 ± 0.357
0.0IleXaa: 0.0 ± 0.0
Lys
6.483LysAla: 6.483 ± 0.818
0.784LysCys: 0.784 ± 0.301
4.132LysAsp: 4.132 ± 0.765
5.415LysGlu: 5.415 ± 0.888
2.066LysPhe: 2.066 ± 0.426
2.779LysGly: 2.779 ± 0.405
1.496LysHis: 1.496 ± 0.422
4.488LysIle: 4.488 ± 0.649
5.272LysLys: 5.272 ± 0.846
5.058LysLeu: 5.058 ± 0.811
2.066LysMet: 2.066 ± 0.393
3.135LysAsn: 3.135 ± 0.552
2.779LysPro: 2.779 ± 0.407
3.206LysGln: 3.206 ± 0.554
3.99LysArg: 3.99 ± 0.528
5.058LysSer: 5.058 ± 0.569
4.702LysThr: 4.702 ± 0.612
3.847LysVal: 3.847 ± 0.56
1.282LysTrp: 1.282 ± 0.295
2.707LysTyr: 2.707 ± 0.359
0.0LysXaa: 0.0 ± 0.0
Leu
6.768LeuAla: 6.768 ± 0.591
0.641LeuCys: 0.641 ± 0.227
5.058LeuAsp: 5.058 ± 0.702
4.702LeuGlu: 4.702 ± 0.549
3.064LeuPhe: 3.064 ± 0.559
5.985LeuGly: 5.985 ± 0.778
0.855LeuHis: 0.855 ± 0.255
3.634LeuIle: 3.634 ± 0.551
5.842LeuLys: 5.842 ± 0.608
4.488LeuLeu: 4.488 ± 0.82
1.71LeuMet: 1.71 ± 0.35
4.132LeuAsn: 4.132 ± 0.55
2.636LeuPro: 2.636 ± 0.387
2.779LeuGln: 2.779 ± 0.42
2.565LeuArg: 2.565 ± 0.446
5.343LeuSer: 5.343 ± 0.609
4.773LeuThr: 4.773 ± 0.669
3.847LeuVal: 3.847 ± 0.455
0.712LeuTrp: 0.712 ± 0.276
1.852LeuTyr: 1.852 ± 0.411
0.0LeuXaa: 0.0 ± 0.0
Met
2.351MetAla: 2.351 ± 0.416
0.142MetCys: 0.142 ± 0.101
0.997MetAsp: 0.997 ± 0.444
1.639MetGlu: 1.639 ± 0.366
0.427MetPhe: 0.427 ± 0.154
1.781MetGly: 1.781 ± 0.343
0.142MetHis: 0.142 ± 0.109
2.209MetIle: 2.209 ± 0.294
1.71MetLys: 1.71 ± 0.377
2.28MetLeu: 2.28 ± 0.569
0.499MetMet: 0.499 ± 0.167
1.354MetAsn: 1.354 ± 0.279
0.641MetPro: 0.641 ± 0.212
1.14MetGln: 1.14 ± 0.282
1.781MetArg: 1.781 ± 0.356
2.565MetSer: 2.565 ± 0.481
1.639MetThr: 1.639 ± 0.348
0.499MetVal: 0.499 ± 0.181
0.285MetTrp: 0.285 ± 0.14
0.57MetTyr: 0.57 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
3.99AsnAla: 3.99 ± 0.536
0.712AsnCys: 0.712 ± 0.237
3.776AsnAsp: 3.776 ± 0.47
3.277AsnGlu: 3.277 ± 0.597
2.565AsnPhe: 2.565 ± 0.434
5.628AsnGly: 5.628 ± 0.736
0.784AsnHis: 0.784 ± 0.215
3.705AsnIle: 3.705 ± 0.485
3.491AsnLys: 3.491 ± 0.512
3.135AsnLeu: 3.135 ± 0.457
0.997AsnMet: 0.997 ± 0.298
2.636AsnAsn: 2.636 ± 0.576
1.496AsnPro: 1.496 ± 0.333
1.924AsnGln: 1.924 ± 0.415
2.636AsnArg: 2.636 ± 0.457
2.85AsnSer: 2.85 ± 0.429
3.064AsnThr: 3.064 ± 0.415
3.491AsnVal: 3.491 ± 0.597
0.427AsnTrp: 0.427 ± 0.211
2.636AsnTyr: 2.636 ± 0.525
0.0AsnXaa: 0.0 ± 0.0
Pro
2.565ProAla: 2.565 ± 0.4
0.499ProCys: 0.499 ± 0.158
2.636ProAsp: 2.636 ± 0.466
2.921ProGlu: 2.921 ± 0.489
1.781ProPhe: 1.781 ± 0.317
0.855ProGly: 0.855 ± 0.236
0.427ProHis: 0.427 ± 0.202
1.852ProIle: 1.852 ± 0.379
2.28ProLys: 2.28 ± 0.439
1.852ProLeu: 1.852 ± 0.24
0.712ProMet: 0.712 ± 0.216
1.282ProAsn: 1.282 ± 0.306
1.14ProPro: 1.14 ± 0.327
1.567ProGln: 1.567 ± 0.405
0.997ProArg: 0.997 ± 0.289
2.636ProSer: 2.636 ± 0.397
2.209ProThr: 2.209 ± 0.358
2.779ProVal: 2.779 ± 0.459
0.427ProTrp: 0.427 ± 0.198
0.855ProTyr: 0.855 ± 0.265
0.0ProXaa: 0.0 ± 0.0
Gln
3.847GlnAla: 3.847 ± 0.597
0.57GlnCys: 0.57 ± 0.243
1.496GlnAsp: 1.496 ± 0.376
2.779GlnGlu: 2.779 ± 0.403
1.14GlnPhe: 1.14 ± 0.283
2.28GlnGly: 2.28 ± 0.433
0.499GlnHis: 0.499 ± 0.147
1.995GlnIle: 1.995 ± 0.335
2.066GlnLys: 2.066 ± 0.417
2.992GlnLeu: 2.992 ± 0.363
0.57GlnMet: 0.57 ± 0.202
2.066GlnAsn: 2.066 ± 0.409
0.926GlnPro: 0.926 ± 0.303
1.781GlnGln: 1.781 ± 0.45
1.567GlnArg: 1.567 ± 0.345
2.636GlnSer: 2.636 ± 0.376
1.781GlnThr: 1.781 ± 0.314
2.636GlnVal: 2.636 ± 0.502
0.641GlnTrp: 0.641 ± 0.198
1.069GlnTyr: 1.069 ± 0.257
0.0GlnXaa: 0.0 ± 0.0
Arg
3.135ArgAla: 3.135 ± 0.488
0.285ArgCys: 0.285 ± 0.129
3.277ArgAsp: 3.277 ± 0.546
2.992ArgGlu: 2.992 ± 0.686
1.781ArgPhe: 1.781 ± 0.368
2.565ArgGly: 2.565 ± 0.384
0.712ArgHis: 0.712 ± 0.202
3.206ArgIle: 3.206 ± 0.524
2.351ArgLys: 2.351 ± 0.397
2.707ArgLeu: 2.707 ± 0.569
1.567ArgMet: 1.567 ± 0.323
1.995ArgAsn: 1.995 ± 0.324
1.567ArgPro: 1.567 ± 0.304
1.71ArgGln: 1.71 ± 0.29
1.71ArgArg: 1.71 ± 0.347
2.707ArgSer: 2.707 ± 0.458
1.924ArgThr: 1.924 ± 0.38
3.064ArgVal: 3.064 ± 0.377
0.356ArgTrp: 0.356 ± 0.152
1.852ArgTyr: 1.852 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
7.196SerAla: 7.196 ± 1.059
0.356SerCys: 0.356 ± 0.151
4.061SerAsp: 4.061 ± 0.588
3.135SerGlu: 3.135 ± 0.438
3.776SerPhe: 3.776 ± 0.628
6.412SerGly: 6.412 ± 0.591
0.641SerHis: 0.641 ± 0.221
5.13SerIle: 5.13 ± 0.721
4.631SerLys: 4.631 ± 0.67
6.27SerLeu: 6.27 ± 0.789
1.995SerMet: 1.995 ± 0.388
3.99SerAsn: 3.99 ± 0.8
2.28SerPro: 2.28 ± 0.435
2.565SerGln: 2.565 ± 0.475
2.992SerArg: 2.992 ± 0.467
5.272SerSer: 5.272 ± 0.642
3.918SerThr: 3.918 ± 0.616
5.7SerVal: 5.7 ± 0.635
0.57SerTrp: 0.57 ± 0.218
2.85SerTyr: 2.85 ± 0.659
0.0SerXaa: 0.0 ± 0.0
Thr
5.272ThrAla: 5.272 ± 0.704
0.356ThrCys: 0.356 ± 0.183
4.631ThrAsp: 4.631 ± 0.692
2.992ThrGlu: 2.992 ± 0.523
1.852ThrPhe: 1.852 ± 0.316
5.7ThrGly: 5.7 ± 0.606
0.712ThrHis: 0.712 ± 0.272
3.634ThrIle: 3.634 ± 0.598
4.773ThrLys: 4.773 ± 0.595
5.201ThrLeu: 5.201 ± 0.702
0.712ThrMet: 0.712 ± 0.246
3.277ThrAsn: 3.277 ± 0.573
2.779ThrPro: 2.779 ± 0.435
1.71ThrGln: 1.71 ± 0.421
2.066ThrArg: 2.066 ± 0.316
4.56ThrSer: 4.56 ± 0.663
3.42ThrThr: 3.42 ± 0.601
3.99ThrVal: 3.99 ± 0.58
0.784ThrTrp: 0.784 ± 0.202
1.425ThrTyr: 1.425 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
6.127ValAla: 6.127 ± 0.644
0.641ValCys: 0.641 ± 0.192
4.916ValAsp: 4.916 ± 0.462
5.272ValGlu: 5.272 ± 0.585
3.349ValPhe: 3.349 ± 0.53
4.061ValGly: 4.061 ± 0.594
0.712ValHis: 0.712 ± 0.234
4.773ValIle: 4.773 ± 0.625
4.346ValLys: 4.346 ± 0.653
2.921ValLeu: 2.921 ± 0.48
1.852ValMet: 1.852 ± 0.364
4.773ValAsn: 4.773 ± 0.719
1.496ValPro: 1.496 ± 0.386
2.422ValGln: 2.422 ± 0.523
2.565ValArg: 2.565 ± 0.416
4.56ValSer: 4.56 ± 0.638
4.631ValThr: 4.631 ± 0.536
3.918ValVal: 3.918 ± 0.687
0.712ValTrp: 0.712 ± 0.196
1.781ValTyr: 1.781 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
1.069TrpAla: 1.069 ± 0.28
0.071TrpCys: 0.071 ± 0.084
0.641TrpAsp: 0.641 ± 0.212
0.427TrpGlu: 0.427 ± 0.142
0.499TrpPhe: 0.499 ± 0.176
1.211TrpGly: 1.211 ± 0.316
0.499TrpHis: 0.499 ± 0.193
0.57TrpIle: 0.57 ± 0.185
0.641TrpLys: 0.641 ± 0.207
1.069TrpLeu: 1.069 ± 0.24
0.641TrpMet: 0.641 ± 0.227
0.356TrpAsn: 0.356 ± 0.157
0.427TrpPro: 0.427 ± 0.145
0.499TrpGln: 0.499 ± 0.215
0.499TrpArg: 0.499 ± 0.213
0.499TrpSer: 0.499 ± 0.193
0.214TrpThr: 0.214 ± 0.129
0.784TrpVal: 0.784 ± 0.243
0.0TrpTrp: 0.0 ± 0.0
0.427TrpTyr: 0.427 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.351TyrAla: 2.351 ± 0.494
0.57TyrCys: 0.57 ± 0.154
2.422TyrAsp: 2.422 ± 0.441
2.137TyrGlu: 2.137 ± 0.356
1.496TyrPhe: 1.496 ± 0.456
2.28TyrGly: 2.28 ± 0.37
0.499TyrHis: 0.499 ± 0.226
2.565TyrIle: 2.565 ± 0.417
1.852TyrLys: 1.852 ± 0.332
2.28TyrLeu: 2.28 ± 0.356
0.499TyrMet: 0.499 ± 0.214
2.422TyrAsn: 2.422 ± 0.351
1.211TyrPro: 1.211 ± 0.337
1.14TyrGln: 1.14 ± 0.277
1.14TyrArg: 1.14 ± 0.277
2.494TyrSer: 2.494 ± 0.459
2.137TyrThr: 2.137 ± 0.427
2.209TyrVal: 2.209 ± 0.417
0.427TyrTrp: 0.427 ± 0.198
1.567TyrTyr: 1.567 ± 0.42
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (14037 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski