Serpentinicella alkaliphila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Serpentinicella

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2U8S7|A0A4R2U8S7_9CLOT ATP-dependent zinc metalloprotease FtsH OS=Serpentinicella alkaliphila OX=1734049 GN=ftsH PE=3 SV=1
MM1 pKa = 7.91DD2 pKa = 4.66SKK4 pKa = 11.39GFTLISVIISLVIVSMLFTGSFQIFDD30 pKa = 3.74ASLKK34 pKa = 10.27IMKK37 pKa = 9.85NNNIQYY43 pKa = 9.69EE44 pKa = 4.43VARR47 pKa = 11.84ANQSVLEE54 pKa = 4.04EE55 pKa = 4.06FKK57 pKa = 10.87AYY59 pKa = 9.74VVAEE63 pKa = 3.98INFEE67 pKa = 4.21DD68 pKa = 5.38FSLNPYY74 pKa = 9.57SVDD77 pKa = 3.67YY78 pKa = 10.27ISEE81 pKa = 4.29IIIEE85 pKa = 4.64PIEE88 pKa = 4.72DD89 pKa = 3.68EE90 pKa = 4.11SSLYY94 pKa = 9.95MGLSSVRR101 pKa = 11.84GFVGSMYY108 pKa = 9.72MYY110 pKa = 10.57RR111 pKa = 11.84IFFINQILDD120 pKa = 3.13NSFYY124 pKa = 11.13YY125 pKa = 10.57EE126 pKa = 4.27EE127 pKa = 4.79LPITAEE133 pKa = 4.03DD134 pKa = 3.94LLINTNLL141 pKa = 3.49

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2TT21|A0A4R2TT21_9CLOT Putative ABC transport system permease protein OS=Serpentinicella alkaliphila OX=1734049 GN=EDD79_100234 PE=4 SV=1
MM1 pKa = 7.89PKK3 pKa = 9.5MKK5 pKa = 9.88SHH7 pKa = 6.63RR8 pKa = 11.84GARR11 pKa = 11.84KK12 pKa = 9.21RR13 pKa = 11.84FKK15 pKa = 10.03KK16 pKa = 9.32TGTGKK21 pKa = 9.97VKK23 pKa = 10.49RR24 pKa = 11.84MQAFTSHH31 pKa = 7.03ILTKK35 pKa = 9.75KK36 pKa = 6.33TAKK39 pKa = 10.02RR40 pKa = 11.84KK41 pKa = 9.25RR42 pKa = 11.84NLRR45 pKa = 11.84KK46 pKa = 8.8ATLVFKK52 pKa = 11.49GEE54 pKa = 3.92AQKK57 pKa = 10.39IARR60 pKa = 11.84LLQFF64 pKa = 4.33

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2966

0

2966

857452

25

2039

289.1

32.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.791 ± 0.04

0.954 ± 0.018

5.183 ± 0.032

7.346 ± 0.048

4.324 ± 0.034

6.586 ± 0.047

1.601 ± 0.02

10.054 ± 0.049

7.805 ± 0.05

9.526 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.02

5.836 ± 0.039

3.101 ± 0.029

2.789 ± 0.024

3.832 ± 0.025

6.146 ± 0.035

5.133 ± 0.032

6.847 ± 0.036

0.674 ± 0.014

3.839 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski