Waddlia chondrophila (strain ATCC VR-1470 / WSU 86-1044)

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Waddliaceae; Waddlia; Waddlia chondrophila

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1919 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6YSB9|D6YSB9_WADCW Putative type III secretion translocase SctQ OS=Waddlia chondrophila (strain ATCC VR-1470 / WSU 86-1044) OX=716544 GN=sctQ1 PE=4 SV=1
MM1 pKa = 7.75LDD3 pKa = 3.73SYY5 pKa = 8.28MQKK8 pKa = 10.16LVKK11 pKa = 10.27EE12 pKa = 4.17MEE14 pKa = 4.28IEE16 pKa = 3.86EE17 pKa = 4.44SLASDD22 pKa = 3.66VPGVYY27 pKa = 9.57QFPIDD32 pKa = 3.96EE33 pKa = 4.5EE34 pKa = 4.61TTILISEE41 pKa = 4.59IPRR44 pKa = 11.84GFEE47 pKa = 4.04LTCSLSACPEE57 pKa = 3.98KK58 pKa = 10.74KK59 pKa = 9.84QEE61 pKa = 4.07EE62 pKa = 4.67FLTSALFANLFSQGTEE78 pKa = 3.91GCILGLDD85 pKa = 3.69AEE87 pKa = 4.85GEE89 pKa = 4.35IVTLSRR95 pKa = 11.84VIDD98 pKa = 3.75YY99 pKa = 10.6EE100 pKa = 3.54IDD102 pKa = 3.57YY103 pKa = 11.45QEE105 pKa = 4.88FRR107 pKa = 11.84DD108 pKa = 3.94LLEE111 pKa = 5.26DD112 pKa = 4.29FVNTAAQWKK121 pKa = 9.68EE122 pKa = 3.44EE123 pKa = 3.68AAYY126 pKa = 9.83YY127 pKa = 11.13ANAA130 pKa = 4.17

Molecular weight: 14.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6YUW6|D6YUW6_WADCW Putative transcriptional regulator OS=Waddlia chondrophila (strain ATCC VR-1470 / WSU 86-1044) OX=716544 GN=wcw_0557 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84KK3 pKa = 9.52PYY5 pKa = 10.29PDD7 pKa = 5.0DD8 pKa = 3.74LTDD11 pKa = 5.27KK12 pKa = 10.02EE13 pKa = 4.4WEE15 pKa = 4.17QIKK18 pKa = 10.19PLLTSSTYY26 pKa = 10.76RR27 pKa = 11.84NAGRR31 pKa = 11.84KK32 pKa = 7.78PKK34 pKa = 10.05HH35 pKa = 5.79SRR37 pKa = 11.84RR38 pKa = 11.84EE39 pKa = 3.69MFNAIFYY46 pKa = 10.25LLRR49 pKa = 11.84TGCQWRR55 pKa = 11.84HH56 pKa = 5.76LPHH59 pKa = 7.52DD60 pKa = 4.61FPPWTAVQGQYY71 pKa = 11.03SRR73 pKa = 11.84WKK75 pKa = 9.16RR76 pKa = 11.84KK77 pKa = 8.79GVFHH81 pKa = 6.53MVHH84 pKa = 6.99DD85 pKa = 3.92YY86 pKa = 10.7LRR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84LRR92 pKa = 11.84ILLGKK97 pKa = 10.37RR98 pKa = 11.84YY99 pKa = 9.01DD100 pKa = 3.46QKK102 pKa = 11.45LCLRR106 pKa = 4.58

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1919

0

1919

658510

30

4637

343.2

38.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.02 ± 0.053

1.285 ± 0.022

4.997 ± 0.038

7.449 ± 0.056

4.71 ± 0.041

6.461 ± 0.055

2.37 ± 0.028

7.011 ± 0.046

6.947 ± 0.053

10.363 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.028

3.954 ± 0.035

4.187 ± 0.032

4.023 ± 0.043

4.897 ± 0.041

6.742 ± 0.039

4.772 ± 0.041

5.95 ± 0.052

1.216 ± 0.021

3.256 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski