Rhizobium sp. CF080

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6W9F4|W6W9F4_9RHIZ Outer membrane protein assembly factor BamA OS=Rhizobium sp. CF080 OX=1144310 GN=bamA PE=3 SV=1
MM1 pKa = 7.38ATNSDD6 pKa = 3.85NFDD9 pKa = 3.43LTSWKK14 pKa = 10.38LCLPIDD20 pKa = 4.21EE21 pKa = 5.54DD22 pKa = 3.95GGTIGTALEE31 pKa = 4.08ILDD34 pKa = 4.03LSGFEE39 pKa = 4.12HH40 pKa = 7.22SSYY43 pKa = 10.8FYY45 pKa = 10.37TADD48 pKa = 3.55DD49 pKa = 3.69GAMVFRR55 pKa = 11.84AITEE59 pKa = 4.08GALTKK64 pKa = 10.05GTTCARR70 pKa = 11.84SEE72 pKa = 3.82LRR74 pKa = 11.84EE75 pKa = 4.11MNGHH79 pKa = 6.33SLASWTLDD87 pKa = 2.92EE88 pKa = 5.27GGTMTATLKK97 pKa = 10.2IDD99 pKa = 4.02EE100 pKa = 5.25APHH103 pKa = 4.97TTNGAEE109 pKa = 3.88GRR111 pKa = 11.84IVVGQIHH118 pKa = 6.48GSEE121 pKa = 4.34DD122 pKa = 3.51EE123 pKa = 4.16LVRR126 pKa = 11.84LYY128 pKa = 10.4WEE130 pKa = 4.65DD131 pKa = 3.85GEE133 pKa = 5.87VYY135 pKa = 10.49FKK137 pKa = 10.99SDD139 pKa = 3.71KK140 pKa = 11.53GGDD143 pKa = 3.8GNDD146 pKa = 3.38SLKK149 pKa = 10.75FALTNAGGDD158 pKa = 3.66TPQISLGDD166 pKa = 3.59QFSYY170 pKa = 10.66KK171 pKa = 9.55IDD173 pKa = 3.44VHH175 pKa = 7.81GDD177 pKa = 3.12TLTVIVYY184 pKa = 10.71ADD186 pKa = 3.54GDD188 pKa = 4.3TYY190 pKa = 11.49TSVTTVSSAWDD201 pKa = 3.55GEE203 pKa = 4.22QFYY206 pKa = 10.43FKK208 pKa = 10.86AGAYY212 pKa = 9.82LGNNEE217 pKa = 4.44TNSTGAGQVSFYY229 pKa = 11.18GFDD232 pKa = 3.78FSHH235 pKa = 6.07EE236 pKa = 4.28TGEE239 pKa = 4.29GLDD242 pKa = 4.02GLVSTATANDD252 pKa = 3.69DD253 pKa = 3.51SSTYY257 pKa = 10.95SVDD260 pKa = 3.26STGTIGNDD268 pKa = 2.84VLTGGTLADD277 pKa = 3.66VIYY280 pKa = 10.44SYY282 pKa = 11.4GGNDD286 pKa = 3.31VVRR289 pKa = 11.84AGAGDD294 pKa = 3.61DD295 pKa = 3.45RR296 pKa = 11.84VVGGSGADD304 pKa = 3.22KK305 pKa = 11.18LLGQDD310 pKa = 3.45GADD313 pKa = 3.75TIFAGADD320 pKa = 3.32DD321 pKa = 4.26DD322 pKa = 4.61VVYY325 pKa = 10.84GGDD328 pKa = 3.85GDD330 pKa = 4.0DD331 pKa = 4.79TIDD334 pKa = 3.96GGAGNDD340 pKa = 3.82TLKK343 pKa = 11.41GEE345 pKa = 4.42AGANRR350 pKa = 11.84ITGGEE355 pKa = 4.14GDD357 pKa = 3.64DD358 pKa = 4.04TIYY361 pKa = 11.24GGVGADD367 pKa = 4.63DD368 pKa = 4.51LSGDD372 pKa = 3.74AGEE375 pKa = 4.38DD376 pKa = 3.52TIVAGEE382 pKa = 4.3GDD384 pKa = 4.83DD385 pKa = 3.91ILSGGADD392 pKa = 3.37NDD394 pKa = 3.61KK395 pKa = 11.22LYY397 pKa = 11.07GGEE400 pKa = 4.2GADD403 pKa = 3.33RR404 pKa = 11.84LYY406 pKa = 11.28GQGGDD411 pKa = 4.36DD412 pKa = 3.61VLIGQGGADD421 pKa = 3.75KK422 pKa = 11.16LVGADD427 pKa = 4.87GNDD430 pKa = 3.35TLYY433 pKa = 11.03GYY435 pKa = 10.91SDD437 pKa = 3.51NDD439 pKa = 3.55KK440 pKa = 10.99LYY442 pKa = 11.38GDD444 pKa = 4.5AGSDD448 pKa = 3.4KK449 pKa = 11.01LVGGDD454 pKa = 4.92GDD456 pKa = 3.77DD457 pKa = 3.57TLYY460 pKa = 11.36GGAGDD465 pKa = 4.13DD466 pKa = 4.17RR467 pKa = 11.84LYY469 pKa = 11.32ADD471 pKa = 4.43EE472 pKa = 5.4GADD475 pKa = 3.42NLYY478 pKa = 10.99GGTGADD484 pKa = 2.91TFVFTWLDD492 pKa = 3.56PSTLTSGGRR501 pKa = 11.84DD502 pKa = 3.57DD503 pKa = 5.18IYY505 pKa = 11.24QFSEE509 pKa = 4.02ADD511 pKa = 3.1GDD513 pKa = 4.2IINLSGIDD521 pKa = 3.91ANPTVNGGQAFDD533 pKa = 4.92FIGTNAFSEE542 pKa = 4.58QVSEE546 pKa = 3.92LRR548 pKa = 11.84YY549 pKa = 10.24VNTGSEE555 pKa = 4.26TYY557 pKa = 10.01IYY559 pKa = 11.06GDD561 pKa = 3.39INGDD565 pKa = 3.31GAADD569 pKa = 4.06FSIHH573 pKa = 6.01VDD575 pKa = 3.61GVVEE579 pKa = 4.06LQSIDD584 pKa = 4.05FILL587 pKa = 4.79

Molecular weight: 60.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6W082|W6W082_9RHIZ Methyl-accepting chemotaxis sensory transducer OS=Rhizobium sp. CF080 OX=1144310 GN=PMI07_000986 PE=4 SV=1
MM1 pKa = 7.54TNRR4 pKa = 11.84LTRR7 pKa = 11.84RR8 pKa = 11.84LKK10 pKa = 10.56RR11 pKa = 11.84LAPQIIALAVILALITAVAPGFLNVSFQNGRR42 pKa = 11.84LYY44 pKa = 11.19GSLIDD49 pKa = 3.59ILVRR53 pKa = 11.84AAPVALLTIGMTLVIATRR71 pKa = 11.84GIDD74 pKa = 3.57LSIGAVIAICGAVAATLITHH94 pKa = 7.16GYY96 pKa = 7.69PLPVVILISLGVGLLCGLWNGVLVALLDD124 pKa = 3.7IQPIIATLILMVAGRR139 pKa = 11.84GIAQLITEE147 pKa = 4.47GVILTFNNDD156 pKa = 1.97SFAAVGSGSLAGIPIPILIWVCAALIIGLLVRR188 pKa = 11.84RR189 pKa = 11.84SALGFLIEE197 pKa = 3.95ATGINRR203 pKa = 11.84RR204 pKa = 11.84AATLAGVRR212 pKa = 11.84ARR214 pKa = 11.84FLLFFVYY221 pKa = 10.05AISGLCAAIAGLIVTADD238 pKa = 3.08IRR240 pKa = 11.84GADD243 pKa = 3.72ANNAGLWLEE252 pKa = 4.35LDD254 pKa = 4.62AILAVVIGGTSLNGGRR270 pKa = 11.84FSITASLIGALIIQSINTGILVSGFPPEE298 pKa = 3.76FNLIIKK304 pKa = 10.12AGIIMIVLTLQSPAIMALLGFVKK327 pKa = 10.5APRR330 pKa = 11.84RR331 pKa = 11.84KK332 pKa = 10.17AEE334 pKa = 3.96TTSRR338 pKa = 11.84EE339 pKa = 4.05TSHH342 pKa = 6.84KK343 pKa = 11.16AEE345 pKa = 4.03GTVRR349 pKa = 3.53

Molecular weight: 36.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6670

0

6670

2077303

30

5677

311.4

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.616 ± 0.042

0.771 ± 0.009

5.608 ± 0.027

5.753 ± 0.026

3.965 ± 0.022

8.381 ± 0.027

1.977 ± 0.017

5.784 ± 0.027

3.791 ± 0.023

9.898 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.657 ± 0.015

2.892 ± 0.017

5.028 ± 0.022

3.068 ± 0.017

6.582 ± 0.03

5.825 ± 0.018

5.393 ± 0.026

7.379 ± 0.024

1.29 ± 0.012

2.343 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski