Amino acid dipepetide frequency for Rhizobium sp. CF080

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.166AlaAla: 15.166 ± 0.118
0.929AlaCys: 0.929 ± 0.02
6.442AlaAsp: 6.442 ± 0.059
7.184AlaGlu: 7.184 ± 0.076
4.517AlaPhe: 4.517 ± 0.05
9.896AlaGly: 9.896 ± 0.078
2.031AlaHis: 2.031 ± 0.034
6.785AlaIle: 6.785 ± 0.065
4.43AlaLys: 4.43 ± 0.059
11.957AlaLeu: 11.957 ± 0.09
3.574AlaMet: 3.574 ± 0.041
3.05AlaAsn: 3.05 ± 0.043
4.813AlaPro: 4.813 ± 0.062
3.402AlaGln: 3.402 ± 0.043
7.381AlaArg: 7.381 ± 0.069
6.572AlaSer: 6.572 ± 0.066
5.632AlaThr: 5.632 ± 0.053
8.434AlaVal: 8.434 ± 0.064
1.366AlaTrp: 1.366 ± 0.027
2.596AlaTyr: 2.596 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
0.803CysAla: 0.803 ± 0.024
0.102CysCys: 0.102 ± 0.007
0.466CysAsp: 0.466 ± 0.014
0.412CysGlu: 0.412 ± 0.012
0.344CysPhe: 0.344 ± 0.014
0.857CysGly: 0.857 ± 0.022
0.219CysHis: 0.219 ± 0.012
0.416CysIle: 0.416 ± 0.015
0.181CysLys: 0.181 ± 0.01
0.756CysLeu: 0.756 ± 0.019
0.177CysMet: 0.177 ± 0.009
0.213CysAsn: 0.213 ± 0.011
0.352CysPro: 0.352 ± 0.012
0.223CysGln: 0.223 ± 0.012
0.551CysArg: 0.551 ± 0.017
0.434CysSer: 0.434 ± 0.015
0.357CysThr: 0.357 ± 0.015
0.572CysVal: 0.572 ± 0.017
0.091CysTrp: 0.091 ± 0.007
0.189CysTyr: 0.189 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
6.143AspAla: 6.143 ± 0.065
0.435AspCys: 0.435 ± 0.014
2.989AspAsp: 2.989 ± 0.05
3.481AspGlu: 3.481 ± 0.047
2.33AspPhe: 2.33 ± 0.031
4.954AspGly: 4.954 ± 0.07
1.177AspHis: 1.177 ± 0.025
3.535AspIle: 3.535 ± 0.046
1.94AspLys: 1.94 ± 0.03
5.79AspLeu: 5.79 ± 0.05
1.46AspMet: 1.46 ± 0.025
1.494AspAsn: 1.494 ± 0.028
3.345AspPro: 3.345 ± 0.041
1.687AspGln: 1.687 ± 0.029
4.092AspArg: 4.092 ± 0.051
2.065AspSer: 2.065 ± 0.034
2.536AspThr: 2.536 ± 0.042
4.162AspVal: 4.162 ± 0.044
0.907AspTrp: 0.907 ± 0.021
1.555AspTyr: 1.555 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
7.196GluAla: 7.196 ± 0.068
0.335GluCys: 0.335 ± 0.014
2.854GluAsp: 2.854 ± 0.044
3.469GluGlu: 3.469 ± 0.052
1.956GluPhe: 1.956 ± 0.034
4.179GluGly: 4.179 ± 0.052
1.156GluHis: 1.156 ± 0.023
3.87GluIle: 3.87 ± 0.047
2.822GluLys: 2.822 ± 0.043
5.443GluLeu: 5.443 ± 0.055
1.639GluMet: 1.639 ± 0.03
1.899GluAsn: 1.899 ± 0.028
2.706GluPro: 2.706 ± 0.043
1.989GluGln: 1.989 ± 0.032
4.474GluArg: 4.474 ± 0.052
2.319GluSer: 2.319 ± 0.034
3.673GluThr: 3.673 ± 0.049
3.752GluVal: 3.752 ± 0.05
0.71GluTrp: 0.71 ± 0.018
1.089GluTyr: 1.089 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.486PheAla: 4.486 ± 0.051
0.4PheCys: 0.4 ± 0.014
2.753PheAsp: 2.753 ± 0.04
2.32PheGlu: 2.32 ± 0.039
1.6PhePhe: 1.6 ± 0.032
3.883PheGly: 3.883 ± 0.056
0.816PheHis: 0.816 ± 0.021
1.981PheIle: 1.981 ± 0.028
1.238PheLys: 1.238 ± 0.023
3.617PheLeu: 3.617 ± 0.045
0.882PheMet: 0.882 ± 0.023
1.203PheAsn: 1.203 ± 0.028
1.718PhePro: 1.718 ± 0.031
1.151PheGln: 1.151 ± 0.027
2.345PheArg: 2.345 ± 0.036
2.678PheSer: 2.678 ± 0.036
2.072PheThr: 2.072 ± 0.033
2.946PheVal: 2.946 ± 0.038
0.599PheTrp: 0.599 ± 0.02
0.956PheTyr: 0.956 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
8.306GlyAla: 8.306 ± 0.084
0.798GlyCys: 0.798 ± 0.019
4.245GlyAsp: 4.245 ± 0.06
4.79GlyGlu: 4.79 ± 0.058
3.788GlyPhe: 3.788 ± 0.045
6.942GlyGly: 6.942 ± 0.087
1.809GlyHis: 1.809 ± 0.033
5.203GlyIle: 5.203 ± 0.056
3.674GlyLys: 3.674 ± 0.043
8.665GlyLeu: 8.665 ± 0.08
2.4GlyMet: 2.4 ± 0.038
2.408GlyAsn: 2.408 ± 0.049
3.367GlyPro: 3.367 ± 0.044
2.711GlyGln: 2.711 ± 0.041
5.664GlyArg: 5.664 ± 0.057
4.903GlySer: 4.903 ± 0.057
4.631GlyThr: 4.631 ± 0.06
5.856GlyVal: 5.856 ± 0.056
1.247GlyTrp: 1.247 ± 0.026
2.409GlyTyr: 2.409 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.097HisAla: 2.097 ± 0.036
0.197HisCys: 0.197 ± 0.009
1.198HisAsp: 1.198 ± 0.029
1.065HisGlu: 1.065 ± 0.023
0.886HisPhe: 0.886 ± 0.021
1.851HisGly: 1.851 ± 0.027
0.555HisHis: 0.555 ± 0.02
1.061HisIle: 1.061 ± 0.023
0.522HisLys: 0.522 ± 0.015
1.983HisLeu: 1.983 ± 0.034
0.543HisMet: 0.543 ± 0.016
0.498HisAsn: 0.498 ± 0.017
1.263HisPro: 1.263 ± 0.027
0.604HisGln: 0.604 ± 0.017
1.304HisArg: 1.304 ± 0.024
1.012HisSer: 1.012 ± 0.023
0.809HisThr: 0.809 ± 0.02
1.494HisVal: 1.494 ± 0.027
0.304HisTrp: 0.304 ± 0.012
0.522HisTyr: 0.522 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
7.776IleAla: 7.776 ± 0.065
0.586IleCys: 0.586 ± 0.016
3.839IleAsp: 3.839 ± 0.042
3.745IleGlu: 3.745 ± 0.047
2.187IlePhe: 2.187 ± 0.031
5.459IleGly: 5.459 ± 0.063
1.01IleHis: 1.01 ± 0.022
2.785IleIle: 2.785 ± 0.048
1.707IleLys: 1.707 ± 0.031
5.087IleLeu: 5.087 ± 0.057
1.189IleMet: 1.189 ± 0.027
1.66IleAsn: 1.66 ± 0.032
2.586IlePro: 2.586 ± 0.039
1.368IleGln: 1.368 ± 0.026
3.622IleArg: 3.622 ± 0.042
3.595IleSer: 3.595 ± 0.04
3.003IleThr: 3.003 ± 0.039
4.664IleVal: 4.664 ± 0.058
0.665IleTrp: 0.665 ± 0.02
1.307IleTyr: 1.307 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
4.769LysAla: 4.769 ± 0.059
0.16LysCys: 0.16 ± 0.008
2.06LysAsp: 2.06 ± 0.035
1.884LysGlu: 1.884 ± 0.033
1.11LysPhe: 1.11 ± 0.024
2.965LysGly: 2.965 ± 0.038
0.693LysHis: 0.693 ± 0.017
2.192LysIle: 2.192 ± 0.027
1.567LysLys: 1.567 ± 0.03
3.846LysLeu: 3.846 ± 0.045
0.948LysMet: 0.948 ± 0.023
1.126LysAsn: 1.126 ± 0.023
2.43LysPro: 2.43 ± 0.04
1.156LysGln: 1.156 ± 0.026
2.508LysArg: 2.508 ± 0.035
2.262LysSer: 2.262 ± 0.031
2.368LysThr: 2.368 ± 0.032
2.732LysVal: 2.732 ± 0.039
0.412LysTrp: 0.412 ± 0.015
0.718LysTyr: 0.718 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
12.336LeuAla: 12.336 ± 0.092
0.809LeuCys: 0.809 ± 0.024
5.877LeuAsp: 5.877 ± 0.055
5.146LeuGlu: 5.146 ± 0.059
3.67LeuPhe: 3.67 ± 0.046
8.082LeuGly: 8.082 ± 0.079
1.731LeuHis: 1.731 ± 0.03
5.454LeuIle: 5.454 ± 0.067
4.239LeuLys: 4.239 ± 0.052
9.215LeuLeu: 9.215 ± 0.092
2.477LeuMet: 2.477 ± 0.038
2.794LeuAsn: 2.794 ± 0.031
5.351LeuPro: 5.351 ± 0.059
2.717LeuGln: 2.717 ± 0.036
6.002LeuArg: 6.002 ± 0.06
6.952LeuSer: 6.952 ± 0.061
5.619LeuThr: 5.619 ± 0.066
7.334LeuVal: 7.334 ± 0.071
1.094LeuTrp: 1.094 ± 0.028
2.085LeuTyr: 2.085 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
3.122MetAla: 3.122 ± 0.041
0.13MetCys: 0.13 ± 0.008
1.254MetAsp: 1.254 ± 0.026
1.246MetGlu: 1.246 ± 0.027
0.787MetPhe: 0.787 ± 0.02
1.794MetGly: 1.794 ± 0.03
0.433MetHis: 0.433 ± 0.014
1.632MetIle: 1.632 ± 0.033
1.197MetLys: 1.197 ± 0.029
2.679MetLeu: 2.679 ± 0.037
0.738MetMet: 0.738 ± 0.02
0.919MetAsn: 0.919 ± 0.026
1.586MetPro: 1.586 ± 0.028
0.888MetGln: 0.888 ± 0.024
1.897MetArg: 1.897 ± 0.031
1.826MetSer: 1.826 ± 0.031
2.027MetThr: 2.027 ± 0.03
1.86MetVal: 1.86 ± 0.034
0.221MetTrp: 0.221 ± 0.009
0.338MetTyr: 0.338 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.266AsnAla: 3.266 ± 0.034
0.243AsnCys: 0.243 ± 0.011
1.536AsnAsp: 1.536 ± 0.037
1.415AsnGlu: 1.415 ± 0.029
1.128AsnPhe: 1.128 ± 0.025
2.697AsnGly: 2.697 ± 0.051
0.564AsnHis: 0.564 ± 0.017
1.645AsnIle: 1.645 ± 0.031
0.845AsnLys: 0.845 ± 0.025
2.905AsnLeu: 2.905 ± 0.039
0.732AsnMet: 0.732 ± 0.022
0.87AsnAsn: 0.87 ± 0.024
2.007AsnPro: 2.007 ± 0.031
0.88AsnGln: 0.88 ± 0.022
1.965AsnArg: 1.965 ± 0.032
1.541AsnSer: 1.541 ± 0.031
1.408AsnThr: 1.408 ± 0.026
2.077AsnVal: 2.077 ± 0.037
0.444AsnTrp: 0.444 ± 0.013
0.747AsnTyr: 0.747 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
5.754ProAla: 5.754 ± 0.068
0.267ProCys: 0.267 ± 0.011
3.409ProAsp: 3.409 ± 0.047
3.512ProGlu: 3.512 ± 0.042
2.068ProPhe: 2.068 ± 0.029
4.24ProGly: 4.24 ± 0.046
1.101ProHis: 1.101 ± 0.023
2.521ProIle: 2.521 ± 0.041
1.958ProLys: 1.958 ± 0.032
4.622ProLeu: 4.622 ± 0.048
1.26ProMet: 1.26 ± 0.024
1.513ProAsn: 1.513 ± 0.028
2.281ProPro: 2.281 ± 0.043
1.63ProGln: 1.63 ± 0.031
2.645ProArg: 2.645 ± 0.046
2.91ProSer: 2.91 ± 0.041
2.459ProThr: 2.459 ± 0.03
4.238ProVal: 4.238 ± 0.052
0.614ProTrp: 0.614 ± 0.02
1.274ProTyr: 1.274 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.773GlnAla: 3.773 ± 0.041
0.173GlnCys: 0.173 ± 0.009
1.383GlnAsp: 1.383 ± 0.027
1.546GlnGlu: 1.546 ± 0.029
1.122GlnPhe: 1.122 ± 0.023
2.251GlnGly: 2.251 ± 0.031
0.593GlnHis: 0.593 ± 0.018
1.923GlnIle: 1.923 ± 0.031
1.297GlnLys: 1.297 ± 0.029
2.777GlnLeu: 2.777 ± 0.036
0.916GlnMet: 0.916 ± 0.022
0.985GlnAsn: 0.985 ± 0.025
1.746GlnPro: 1.746 ± 0.03
1.242GlnGln: 1.242 ± 0.027
2.163GlnArg: 2.163 ± 0.038
1.861GlnSer: 1.861 ± 0.029
1.725GlnThr: 1.725 ± 0.034
2.155GlnVal: 2.155 ± 0.035
0.383GlnTrp: 0.383 ± 0.013
0.665GlnTyr: 0.665 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
6.597ArgAla: 6.597 ± 0.069
0.424ArgCys: 0.424 ± 0.014
3.758ArgAsp: 3.758 ± 0.042
3.97ArgGlu: 3.97 ± 0.051
2.793ArgPhe: 2.793 ± 0.04
4.374ArgGly: 4.374 ± 0.047
1.582ArgHis: 1.582 ± 0.028
4.141ArgIle: 4.141 ± 0.042
2.616ArgLys: 2.616 ± 0.037
7.014ArgLeu: 7.014 ± 0.061
1.937ArgMet: 1.937 ± 0.032
2.016ArgAsn: 2.016 ± 0.03
3.195ArgPro: 3.195 ± 0.046
2.597ArgGln: 2.597 ± 0.04
4.958ArgArg: 4.958 ± 0.06
3.747ArgSer: 3.747 ± 0.045
3.159ArgThr: 3.159 ± 0.04
4.277ArgVal: 4.277 ± 0.044
0.906ArgTrp: 0.906 ± 0.023
1.761ArgTyr: 1.761 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
6.329SerAla: 6.329 ± 0.058
0.384SerCys: 0.384 ± 0.015
3.089SerAsp: 3.089 ± 0.041
3.118SerGlu: 3.118 ± 0.039
2.618SerPhe: 2.618 ± 0.041
5.847SerGly: 5.847 ± 0.064
1.148SerHis: 1.148 ± 0.027
3.313SerIle: 3.313 ± 0.043
1.979SerLys: 1.979 ± 0.031
5.846SerLeu: 5.846 ± 0.058
1.521SerMet: 1.521 ± 0.022
1.607SerAsn: 1.607 ± 0.035
2.882SerPro: 2.882 ± 0.042
1.741SerGln: 1.741 ± 0.031
3.795SerArg: 3.795 ± 0.044
3.464SerSer: 3.464 ± 0.043
3.005SerThr: 3.005 ± 0.04
4.36SerVal: 4.36 ± 0.052
0.785SerTrp: 0.785 ± 0.018
1.425SerTyr: 1.425 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
6.117ThrAla: 6.117 ± 0.058
0.399ThrCys: 0.399 ± 0.015
2.798ThrAsp: 2.798 ± 0.042
2.83ThrGlu: 2.83 ± 0.038
2.193ThrPhe: 2.193 ± 0.031
5.043ThrGly: 5.043 ± 0.057
1.028ThrHis: 1.028 ± 0.021
3.29ThrIle: 3.29 ± 0.047
1.692ThrLys: 1.692 ± 0.03
5.622ThrLeu: 5.622 ± 0.055
1.311ThrMet: 1.311 ± 0.027
1.424ThrAsn: 1.424 ± 0.023
3.049ThrPro: 3.049 ± 0.044
1.399ThrGln: 1.399 ± 0.026
3.204ThrArg: 3.204 ± 0.04
3.101ThrSer: 3.101 ± 0.04
2.898ThrThr: 2.898 ± 0.04
4.517ThrVal: 4.517 ± 0.046
0.676ThrTrp: 0.676 ± 0.021
1.336ThrTyr: 1.336 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
8.61ValAla: 8.61 ± 0.07
0.61ValCys: 0.61 ± 0.017
3.985ValAsp: 3.985 ± 0.046
4.534ValGlu: 4.534 ± 0.049
2.985ValPhe: 2.985 ± 0.037
5.433ValGly: 5.433 ± 0.056
1.345ValHis: 1.345 ± 0.025
4.356ValIle: 4.356 ± 0.051
2.694ValLys: 2.694 ± 0.04
7.233ValLeu: 7.233 ± 0.075
1.991ValMet: 1.991 ± 0.032
2.084ValAsn: 2.084 ± 0.035
3.717ValPro: 3.717 ± 0.041
2.0ValGln: 2.0 ± 0.034
4.509ValArg: 4.509 ± 0.048
4.885ValSer: 4.885 ± 0.052
4.592ValThr: 4.592 ± 0.043
5.879ValVal: 5.879 ± 0.058
0.855ValTrp: 0.855 ± 0.02
1.493ValTyr: 1.493 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.089TrpAla: 1.089 ± 0.023
0.122TrpCys: 0.122 ± 0.009
0.627TrpAsp: 0.627 ± 0.018
0.581TrpGlu: 0.581 ± 0.017
0.536TrpPhe: 0.536 ± 0.018
0.887TrpGly: 0.887 ± 0.024
0.307TrpHis: 0.307 ± 0.013
0.693TrpIle: 0.693 ± 0.022
0.545TrpLys: 0.545 ± 0.019
1.552TrpLeu: 1.552 ± 0.031
0.375TrpMet: 0.375 ± 0.014
0.484TrpAsn: 0.484 ± 0.015
0.671TrpPro: 0.671 ± 0.021
0.541TrpGln: 0.541 ± 0.017
1.0TrpArg: 1.0 ± 0.023
0.808TrpSer: 0.808 ± 0.021
0.771TrpThr: 0.771 ± 0.019
0.789TrpVal: 0.789 ± 0.019
0.227TrpTrp: 0.227 ± 0.012
0.29TrpTyr: 0.29 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.42TyrAla: 2.42 ± 0.037
0.212TyrCys: 0.212 ± 0.011
1.515TyrAsp: 1.515 ± 0.031
1.284TyrGlu: 1.284 ± 0.029
1.027TyrPhe: 1.027 ± 0.023
2.18TyrGly: 2.18 ± 0.031
0.475TyrHis: 0.475 ± 0.016
1.043TyrIle: 1.043 ± 0.022
0.762TyrLys: 0.762 ± 0.021
2.377TyrLeu: 2.377 ± 0.035
0.503TyrMet: 0.503 ± 0.014
0.667TyrAsn: 0.667 ± 0.019
1.211TyrPro: 1.211 ± 0.026
0.752TyrGln: 0.752 ± 0.02
1.745TyrArg: 1.745 ± 0.034
1.32TyrSer: 1.32 ± 0.029
1.182TyrThr: 1.182 ± 0.027
1.695TyrVal: 1.695 ± 0.026
0.389TyrTrp: 0.389 ± 0.012
0.671TyrTyr: 0.671 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6670 proteins (2077303 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski