Aspergillus nomiae NRRL 13137
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11914 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L1JGA0|A0A0L1JGA0_ASPNO Peroxisomal hydratase-dehydrogenase-epimerase OS=Aspergillus nomiae NRRL 13137 OX=1509407 GN=ANOM_001084 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 PYY4 pKa = 11.25 SLLSSLAILGPAALTVAQTLNIVAHH29 pKa = 6.27 QDD31 pKa = 3.27 DD32 pKa = 5.27 DD33 pKa = 4.76 LLFMSPDD40 pKa = 3.31 LLSEE44 pKa = 4.24 VRR46 pKa = 11.84 SGRR49 pKa = 11.84 AVRR52 pKa = 11.84 TVFLTAGDD60 pKa = 3.83 AGNGEE65 pKa = 5.03 DD66 pKa = 3.87 YY67 pKa = 7.67 WTSRR71 pKa = 11.84 QAGSLATYY79 pKa = 9.88 AQIAGVANEE88 pKa = 4.23 WNEE91 pKa = 3.36 GDD93 pKa = 3.81 AGIEE97 pKa = 4.1 GFDD100 pKa = 3.28 IPVYY104 pKa = 10.26 EE105 pKa = 5.35 LAAQPQIEE113 pKa = 4.62 LAFLHH118 pKa = 6.87 IPDD121 pKa = 4.77 GNLDD125 pKa = 3.51 GSGFASTGSVSLQKK139 pKa = 10.6 LWEE142 pKa = 4.16 GTIDD146 pKa = 4.27 QIGTVDD152 pKa = 3.93 ASGTTYY158 pKa = 10.16 TRR160 pKa = 11.84 QQLLDD165 pKa = 3.66 VLSDD169 pKa = 3.65 IIEE172 pKa = 4.39 NFSPDD177 pKa = 4.18 RR178 pKa = 11.84 INTLDD183 pKa = 3.59 FVNDD187 pKa = 3.65 IGDD190 pKa = 4.05 GDD192 pKa = 4.26 HH193 pKa = 7.15 SDD195 pKa = 3.52 HH196 pKa = 6.12 YY197 pKa = 9.18 TTGFFADD204 pKa = 4.37 HH205 pKa = 7.14 ASQAADD211 pKa = 3.22 NNADD215 pKa = 3.27 FFGYY219 pKa = 8.88 MGYY222 pKa = 9.61 PVASLPANLSPDD234 pKa = 3.44 QIADD238 pKa = 3.44 KK239 pKa = 10.91 KK240 pKa = 11.12 AIFYY244 pKa = 9.38 FYY246 pKa = 10.9 AGYY249 pKa = 10.77 DD250 pKa = 3.43 SGTCNSDD257 pKa = 3.21 AACAGRR263 pKa = 11.84 PEE265 pKa = 4.35 LAWLEE270 pKa = 4.0 RR271 pKa = 11.84 QYY273 pKa = 11.48 QVV275 pKa = 3.32
Molecular weight: 29.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.694
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.745
Grimsley 3.605
Solomon 3.923
Lehninger 3.872
Nozaki 4.037
DTASelect 4.304
Thurlkill 3.757
EMBOSS 3.884
Sillero 4.05
Patrickios 1.24
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A0L1ILW8|A0A0L1ILW8_ASPNO Arginine permease OS=Aspergillus nomiae NRRL 13137 OX=1509407 GN=ANOM_011281 PE=4 SV=1
LL1 pKa = 7.45 LLPSLLPPLLLPSLLPPLLRR21 pKa = 11.84 LGLLLLLLRR30 pKa = 11.84 LGLLLQRR37 pKa = 11.84 RR38 pKa = 11.84 LLSLLPLLRR47 pKa = 11.84 PMFPPFSLPLVVPVFLASLLSPAPLLSRR75 pKa = 11.84 LPPLRR80 pKa = 11.84 PSPVSPLSSQLFQVLTLPLLPP101 pKa = 5.06
Molecular weight: 11.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11914
0
11914
5973996
11
7584
501.4
55.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.334 ± 0.018
1.311 ± 0.008
5.598 ± 0.015
6.053 ± 0.022
3.818 ± 0.014
6.794 ± 0.019
2.446 ± 0.008
5.113 ± 0.016
4.553 ± 0.018
9.242 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.164 ± 0.008
3.679 ± 0.011
5.9 ± 0.024
4.033 ± 0.013
5.996 ± 0.018
8.268 ± 0.022
5.955 ± 0.013
6.294 ± 0.015
1.519 ± 0.009
2.93 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here